Why 3‐D? Gel‐based microarrays in proteomics
Identifieur interne : 002A47 ( Main/Curation ); précédent : 002A46; suivant : 002A48Why 3‐D? Gel‐based microarrays in proteomics
Auteurs : Alla Yu. Rubina [Russie] ; Alexander Kolchinsky [États-Unis] ; Alexander A. Makarov [Russie] ; Alexander S. Zasedatelev [Russie]Source :
- PROTEOMICS [ 1615-9853 ] ; 2008-02.
Abstract
Gel‐based microarrays (biochips) consisting of nanoliter and sub‐nanoliter gel drops on hydrophobic substrate are a versatile technology platform for immobilization of proteins and other biopolymers. Biochips provide a highly hydrophilic environment, which stabilizes immobilized molecules and facilitates their interactions with analytes. The probes are immobilized simultaneously with gel polymerization, evenly distributed throughout individual elements, and are easily accessible because of large pores. Each element is an isolated nanotube. Applications of biochips in the studies of protein interactions with other proteins, nucleic acids, and glycans are described. In particular, biochips are compatible with MALDI‐MS. Biochip‐based assay of prostate‐specific antigen became the first protein microarray approved for clinical use by a national regulatory agency. In this review, 3‐D immobilization is compared with mainstream technologies based on surface immobilization.
Url:
DOI: 10.1002/pmic.200700629
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002393
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :002393
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000761
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :002A73
Links to Exploration step
ISTEX:D0722B540E07B79B025C0DE644A9CCDB97991341Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Why 3‐D? Gel‐based microarrays in proteomics</title>
<author><name sortKey="Rubina, Alla Yu" sort="Rubina, Alla Yu" uniqKey="Rubina A" first="Alla Yu." last="Rubina">Alla Yu. Rubina</name>
</author>
<author><name sortKey="Kolchinsky, Alexander" sort="Kolchinsky, Alexander" uniqKey="Kolchinsky A" first="Alexander" last="Kolchinsky">Alexander Kolchinsky</name>
</author>
<author><name sortKey="Makarov, Alexander A" sort="Makarov, Alexander A" uniqKey="Makarov A" first="Alexander A." last="Makarov">Alexander A. Makarov</name>
</author>
<author><name sortKey="Zasedatelev, Alexander S" sort="Zasedatelev, Alexander S" uniqKey="Zasedatelev A" first="Alexander S." last="Zasedatelev">Alexander S. Zasedatelev</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:D0722B540E07B79B025C0DE644A9CCDB97991341</idno>
<date when="2008" year="2008">2008</date>
<idno type="doi">10.1002/pmic.200700629</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-BNRTS3TT-8/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002393</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002393</idno>
<idno type="wicri:Area/Istex/Curation">002393</idno>
<idno type="wicri:Area/Istex/Checkpoint">000761</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000761</idno>
<idno type="wicri:doubleKey">1615-9853:2008:Rubina A:why:d:gel</idno>
<idno type="wicri:Area/Main/Merge">002A73</idno>
<idno type="wicri:Area/Main/Curation">002A47</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Why 3‐D? Gel‐based microarrays in proteomics</title>
<author><name sortKey="Rubina, Alla Yu" sort="Rubina, Alla Yu" uniqKey="Rubina A" first="Alla Yu." last="Rubina">Alla Yu. Rubina</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Engelhardt Institute of Molecular Biology of Russian Academy of Sciences, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kolchinsky, Alexander" sort="Kolchinsky, Alexander" uniqKey="Kolchinsky A" first="Alexander" last="Kolchinsky">Alexander Kolchinsky</name>
<affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Health Front Line, Ltd., Champaign, IL</wicri:regionArea>
<wicri:noRegion>IL</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Makarov, Alexander A" sort="Makarov, Alexander A" uniqKey="Makarov A" first="Alexander A." last="Makarov">Alexander A. Makarov</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Engelhardt Institute of Molecular Biology of Russian Academy of Sciences, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Russie</country>
</affiliation>
</author>
<author><name sortKey="Zasedatelev, Alexander S" sort="Zasedatelev, Alexander S" uniqKey="Zasedatelev A" first="Alexander S." last="Zasedatelev">Alexander S. Zasedatelev</name>
<affiliation wicri:level="3"><country xml:lang="fr">Russie</country>
<wicri:regionArea>Engelhardt Institute of Molecular Biology of Russian Academy of Sciences, Moscow</wicri:regionArea>
<placeName><settlement type="city">Moscou</settlement>
<region>District fédéral central</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Russie</country>
</affiliation>
<affiliation></affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">PROTEOMICS</title>
<title level="j" type="sub">REVIEWS 2008</title>
<title level="j" type="alt">PROTEOMICS</title>
<idno type="ISSN">1615-9853</idno>
<idno type="eISSN">1615-9861</idno>
<imprint><biblScope unit="vol">8</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="817">817</biblScope>
<biblScope unit="page" to="831">831</biblScope>
<biblScope unit="page-count">15</biblScope>
<publisher>WILEY‐VCH Verlag</publisher>
<pubPlace>Weinheim</pubPlace>
<date type="published" when="2008-02">2008-02</date>
</imprint>
<idno type="ISSN">1615-9853</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1615-9853</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Gel‐based microarrays (biochips) consisting of nanoliter and sub‐nanoliter gel drops on hydrophobic substrate are a versatile technology platform for immobilization of proteins and other biopolymers. Biochips provide a highly hydrophilic environment, which stabilizes immobilized molecules and facilitates their interactions with analytes. The probes are immobilized simultaneously with gel polymerization, evenly distributed throughout individual elements, and are easily accessible because of large pores. Each element is an isolated nanotube. Applications of biochips in the studies of protein interactions with other proteins, nucleic acids, and glycans are described. In particular, biochips are compatible with MALDI‐MS. Biochip‐based assay of prostate‐specific antigen became the first protein microarray approved for clinical use by a national regulatory agency. In this review, 3‐D immobilization is compared with mainstream technologies based on surface immobilization.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A47 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002A47 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Curation |type= RBID |clé= ISTEX:D0722B540E07B79B025C0DE644A9CCDB97991341 |texte= Why 3‐D? Gel‐based microarrays in proteomics }}
This area was generated with Dilib version V0.6.33. |