A family of kassinatuerin-2 related peptides from the skin secretion of the African hyperoliid frog, Kassina maculata.
Identifieur interne : 002818 ( Main/Curation ); précédent : 002817; suivant : 002819A family of kassinatuerin-2 related peptides from the skin secretion of the African hyperoliid frog, Kassina maculata.
Auteurs : Lei Wang [Royaume-Uni] ; Mei Zhou ; Stephanie Mcgrath ; Tianbao Chen ; Sean P. Gorman ; Brian Walker ; Chris ShawSource :
- Peptides [ 1873-5169 ] ; 2009.
Descripteurs français
- KwdFr :
- Alignement de séquences, Animaux, Anti-infectieux (pharmacologie), Anura (métabolisme), Candida albicans (), Chromatographie en phase liquide à haute performance, Données de séquences moléculaires, Escherichia coli (), Hémolyse (), Peau (métabolisme), Peptides (), Peptides (isolement et purification), Peptides (pharmacologie), Protéines d'amphibien (), Protéines d'amphibien (isolement et purification), Protéines d'amphibien (pharmacologie), Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Tests de sensibilité microbienne.
- MESH :
- isolement et purification : Peptides, Protéines d'amphibien.
- métabolisme : Anura, Peau.
- pharmacologie : Anti-infectieux, Peptides, Protéines d'amphibien.
- Alignement de séquences, Animaux, Candida albicans, Chromatographie en phase liquide à haute performance, Données de séquences moléculaires, Escherichia coli, Hémolyse, Peptides, Protéines d'amphibien, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Tests de sensibilité microbienne.
English descriptors
- KwdEn :
- Amino Acid Sequence, Amphibian Proteins (chemistry), Amphibian Proteins (isolation & purification), Amphibian Proteins (pharmacology), Animals, Anti-Infective Agents (pharmacology), Anura (metabolism), Base Sequence, Candida albicans (drug effects), Chromatography, High Pressure Liquid, Escherichia coli (drug effects), Hemolysis (drug effects), Microbial Sensitivity Tests, Molecular Sequence Data, Peptides (chemistry), Peptides (isolation & purification), Peptides (pharmacology), Sequence Alignment, Sequence Homology, Amino Acid, Skin (metabolism).
- MESH :
- chemical , chemistry : Amphibian Proteins, Peptides.
- chemical , isolation & purification : Amphibian Proteins, Peptides.
- chemical , pharmacology : Amphibian Proteins, Anti-Infective Agents, Peptides.
- drug effects : Candida albicans, Escherichia coli, Hemolysis.
- metabolism : Anura, Skin.
- Amino Acid Sequence, Animals, Base Sequence, Chromatography, High Pressure Liquid, Microbial Sensitivity Tests, Molecular Sequence Data, Sequence Alignment, Sequence Homology, Amino Acid.
Abstract
We describe the isolation and structural characterization of a family of antimicrobial peptides related to kassinatuerin-2, from the skin secretion of the African hyperoliid frog, Kassina maculata. All four peptides, designated kassinatuerin-2Ma through Md, are C-terminally-amidated 20-mers with the consensus sequence - FX(1)GAIAAALPHVIX(2)AIKNAL - where X(1)=L/F/V/I and X2=S/N. All four peptides are encoded by precursors of 69 amino acids. Synthetic replicates of all kassinatuerin-2 related peptides displayed a potent inhibitory activity against Staphylococcus aureus with a minimal inhibitory concentration of 16microM, at which concentration, however, they effected 18% haemolysis of horse erythrocytes after 2h. Despite obvious membranolytic properties, all peptides were ineffective at inhibiting the growth of Escherichia coli at concentrations up to 200microM and were relatively ineffective against Candida albicans (MIC 120microM). The kassinatuerin-2 related peptides of K. maculata skin secretion thus possess a discrete antimicrobial and weak haemolytic activity in contrast to the prototype kassinatuerin-2 from the skin secretion of Kassina senegalensis.
DOI: 10.1016/j.peptides.2009.04.021
PubMed: 19427345
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002016
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :002016
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001F35
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000686
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000686
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000686
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :002844
Links to Exploration step
pubmed:19427345Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A family of kassinatuerin-2 related peptides from the skin secretion of the African hyperoliid frog, Kassina maculata.</title>
<author><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Therapeutics Research, School of Pharmacy, Queen's University, Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Molecular Therapeutics Research, School of Pharmacy, Queen's University, Lisburn Road, Belfast BT9 7BL, Northern Ireland</wicri:regionArea>
<wicri:noRegion>Northern Ireland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Mei" sort="Zhou, Mei" uniqKey="Zhou M" first="Mei" last="Zhou">Mei Zhou</name>
</author>
<author><name sortKey="Mcgrath, Stephanie" sort="Mcgrath, Stephanie" uniqKey="Mcgrath S" first="Stephanie" last="Mcgrath">Stephanie Mcgrath</name>
</author>
<author><name sortKey="Chen, Tianbao" sort="Chen, Tianbao" uniqKey="Chen T" first="Tianbao" last="Chen">Tianbao Chen</name>
</author>
<author><name sortKey="Gorman, Sean P" sort="Gorman, Sean P" uniqKey="Gorman S" first="Sean P" last="Gorman">Sean P. Gorman</name>
</author>
<author><name sortKey="Walker, Brian" sort="Walker, Brian" uniqKey="Walker B" first="Brian" last="Walker">Brian Walker</name>
</author>
<author><name sortKey="Shaw, Chris" sort="Shaw, Chris" uniqKey="Shaw C" first="Chris" last="Shaw">Chris Shaw</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2009">2009</date>
<idno type="RBID">pubmed:19427345</idno>
<idno type="pmid">19427345</idno>
<idno type="doi">10.1016/j.peptides.2009.04.021</idno>
<idno type="wicri:Area/PubMed/Corpus">002016</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002016</idno>
<idno type="wicri:Area/PubMed/Curation">002016</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002016</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001F35</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001F35</idno>
<idno type="wicri:Area/Ncbi/Merge">000686</idno>
<idno type="wicri:Area/Ncbi/Curation">000686</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000686</idno>
<idno type="wicri:Area/Main/Merge">002844</idno>
<idno type="wicri:Area/Main/Curation">002818</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A family of kassinatuerin-2 related peptides from the skin secretion of the African hyperoliid frog, Kassina maculata.</title>
<author><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Molecular Therapeutics Research, School of Pharmacy, Queen's University, Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Molecular Therapeutics Research, School of Pharmacy, Queen's University, Lisburn Road, Belfast BT9 7BL, Northern Ireland</wicri:regionArea>
<wicri:noRegion>Northern Ireland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhou, Mei" sort="Zhou, Mei" uniqKey="Zhou M" first="Mei" last="Zhou">Mei Zhou</name>
</author>
<author><name sortKey="Mcgrath, Stephanie" sort="Mcgrath, Stephanie" uniqKey="Mcgrath S" first="Stephanie" last="Mcgrath">Stephanie Mcgrath</name>
</author>
<author><name sortKey="Chen, Tianbao" sort="Chen, Tianbao" uniqKey="Chen T" first="Tianbao" last="Chen">Tianbao Chen</name>
</author>
<author><name sortKey="Gorman, Sean P" sort="Gorman, Sean P" uniqKey="Gorman S" first="Sean P" last="Gorman">Sean P. Gorman</name>
</author>
<author><name sortKey="Walker, Brian" sort="Walker, Brian" uniqKey="Walker B" first="Brian" last="Walker">Brian Walker</name>
</author>
<author><name sortKey="Shaw, Chris" sort="Shaw, Chris" uniqKey="Shaw C" first="Chris" last="Shaw">Chris Shaw</name>
</author>
</analytic>
<series><title level="j">Peptides</title>
<idno type="eISSN">1873-5169</idno>
<imprint><date when="2009" type="published">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Amphibian Proteins (chemistry)</term>
<term>Amphibian Proteins (isolation & purification)</term>
<term>Amphibian Proteins (pharmacology)</term>
<term>Animals</term>
<term>Anti-Infective Agents (pharmacology)</term>
<term>Anura (metabolism)</term>
<term>Base Sequence</term>
<term>Candida albicans (drug effects)</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Escherichia coli (drug effects)</term>
<term>Hemolysis (drug effects)</term>
<term>Microbial Sensitivity Tests</term>
<term>Molecular Sequence Data</term>
<term>Peptides (chemistry)</term>
<term>Peptides (isolation & purification)</term>
<term>Peptides (pharmacology)</term>
<term>Sequence Alignment</term>
<term>Sequence Homology, Amino Acid</term>
<term>Skin (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term>
<term>Animaux</term>
<term>Anti-infectieux (pharmacologie)</term>
<term>Anura (métabolisme)</term>
<term>Candida albicans ()</term>
<term>Chromatographie en phase liquide à haute performance</term>
<term>Données de séquences moléculaires</term>
<term>Escherichia coli ()</term>
<term>Hémolyse ()</term>
<term>Peau (métabolisme)</term>
<term>Peptides ()</term>
<term>Peptides (isolement et purification)</term>
<term>Peptides (pharmacologie)</term>
<term>Protéines d'amphibien ()</term>
<term>Protéines d'amphibien (isolement et purification)</term>
<term>Protéines d'amphibien (pharmacologie)</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Tests de sensibilité microbienne</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Amphibian Proteins</term>
<term>Peptides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Amphibian Proteins</term>
<term>Peptides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Amphibian Proteins</term>
<term>Anti-Infective Agents</term>
<term>Peptides</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Candida albicans</term>
<term>Escherichia coli</term>
<term>Hemolysis</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Peptides</term>
<term>Protéines d'amphibien</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Anura</term>
<term>Skin</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anura</term>
<term>Peau</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Anti-infectieux</term>
<term>Peptides</term>
<term>Protéines d'amphibien</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Microbial Sensitivity Tests</term>
<term>Molecular Sequence Data</term>
<term>Sequence Alignment</term>
<term>Sequence Homology, Amino Acid</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Animaux</term>
<term>Candida albicans</term>
<term>Chromatographie en phase liquide à haute performance</term>
<term>Données de séquences moléculaires</term>
<term>Escherichia coli</term>
<term>Hémolyse</term>
<term>Peptides</term>
<term>Protéines d'amphibien</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Tests de sensibilité microbienne</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We describe the isolation and structural characterization of a family of antimicrobial peptides related to kassinatuerin-2, from the skin secretion of the African hyperoliid frog, Kassina maculata. All four peptides, designated kassinatuerin-2Ma through Md, are C-terminally-amidated 20-mers with the consensus sequence - FX(1)GAIAAALPHVIX(2)AIKNAL - where X(1)=L/F/V/I and X2=S/N. All four peptides are encoded by precursors of 69 amino acids. Synthetic replicates of all kassinatuerin-2 related peptides displayed a potent inhibitory activity against Staphylococcus aureus with a minimal inhibitory concentration of 16microM, at which concentration, however, they effected 18% haemolysis of horse erythrocytes after 2h. Despite obvious membranolytic properties, all peptides were ineffective at inhibiting the growth of Escherichia coli at concentrations up to 200microM and were relatively ineffective against Candida albicans (MIC 120microM). The kassinatuerin-2 related peptides of K. maculata skin secretion thus possess a discrete antimicrobial and weak haemolytic activity in contrast to the prototype kassinatuerin-2 from the skin secretion of Kassina senegalensis.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002818 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002818 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:19427345 |texte= A family of kassinatuerin-2 related peptides from the skin secretion of the African hyperoliid frog, Kassina maculata. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:19427345" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |