Development and validation of a flax (Linum usitatissimum L.) gene expression oligo microarray
Identifieur interne : 002645 ( Main/Curation ); précédent : 002644; suivant : 002646Development and validation of a flax (Linum usitatissimum L.) gene expression oligo microarray
Auteurs : Stéphane Fenart [France] ; Yves-Placide Assoumou Ndong [France] ; Jorge Duarte [France] ; Nathalie Rivière [France] ; Jeroen Wilmer [France] ; Olivier Van Wuytswinkel [France] ; Anca Lucau [France] ; Emmanuelle Cariou [France] ; Godfrey Neutelings [France] ; Laurent Gutierrez [France] ; Brigitte Chabbert [France] ; Xavier Guillot [France] ; Reynald Tavernier [France] ; Simon Hawkins [France] ; Brigitte Thomasset [France]Source :
- BMC Genomics [ 1471-2164 ] ; 2010.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes, Analyse de séquence d'ADN, Analyse en composantes principales, Annotation de séquence moléculaire, Cartographie de contigs, Gènes de plante (génétique), Génotype, Lin (croissance et développement), Lin (génétique), RT-PCR, Reproductibilité des résultats, Régulation de l'expression des gènes au cours du développement, Régulation de l'expression des gènes végétaux, Spécificité d'organe (génétique), Séquençage par oligonucléotides en batterie (), Tiges de plante (génétique).
- MESH :
- croissance et développement : Lin.
- génétique : Gènes de plante, Lin, Spécificité d'organe, Tiges de plante.
- Analyse de profil d'expression de gènes, Analyse de séquence d'ADN, Analyse en composantes principales, Annotation de séquence moléculaire, Cartographie de contigs, Génotype, RT-PCR, Reproductibilité des résultats, Régulation de l'expression des gènes au cours du développement, Régulation de l'expression des gènes végétaux, Séquençage par oligonucléotides en batterie.
English descriptors
- KwdEn :
- Contig Mapping, Flax (genetics), Flax (growth & development), Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Genes, Plant (genetics), Genotype, Molecular Sequence Annotation, Oligonucleotide Array Sequence Analysis (methods), Organ Specificity (genetics), Plant Stems (genetics), Principal Component Analysis, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
- MESH :
- genetics : Flax, Genes, Plant, Organ Specificity, Plant Stems.
- growth & development : Flax.
- methods : Oligonucleotide Array Sequence Analysis.
- Contig Mapping, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Genotype, Molecular Sequence Annotation, Principal Component Analysis, Reproducibility of Results, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
Flax (
Nine different RNA samples obtained from flax inner- and outer-stems, seeds, leaves and roots were used to generate a collection of 1,066,481 ESTs by massive parallel pyrosequencing. Sequences were assembled into 59,626 unigenes and 48,021 sequences were selected for oligo design and high-density microarray (Nimblegen 385K) fabrication with eight, non-overlapping 25-mers oligos per unigene. 18 independent experiments were used to evaluate the hybridization quality, precision, specificity and accuracy and all results confirmed the high technical quality of our microarray platform. Cross-validation of microarray data was carried out using quantitative qRT-PCR. Nine target genes were selected on the basis of microarray results and reflected the whole range of fold change (both up-regulated and down-regulated genes in different samples). A statistically significant positive correlation was obtained comparing expression levels for each target gene across all biological replicates both in qRT-PCR and microarray results. Further experiments illustrated the capacity of our arrays to detect differential gene expression in a variety of flax tissues as well as between two contrasted flax varieties.
All results suggest that our high-density flax oligo-microarray platform can be used as a very sensitive tool for analyzing gene expression in a large variety of tissues as well as in different cultivars. Moreover, this highly reliable platform can also be used for the quantification of mRNA transcriptional profiling in different flax tissues.
Url:
DOI: 10.1186/1471-2164-11-592
PubMed: 20964859
PubMed Central: 3091737
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000A94
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000A94
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001360
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001F29
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001F29
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001E55
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000782
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000782
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000782
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :002670
Links to Exploration step
PMC:3091737Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development and validation of a flax (<italic>Linum usitatissimum </italic>
L.) gene expression oligo microarray</title>
<author><name sortKey="Fenart, Stephane" sort="Fenart, Stephane" uniqKey="Fenart S" first="Stéphane" last="Fenart">Stéphane Fenart</name>
<affiliation wicri:level="3"><nlm:aff id="I1">Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ndong, Yves Placide Assoumou" sort="Ndong, Yves Placide Assoumou" uniqKey="Ndong Y" first="Yves-Placide Assoumou" last="Ndong">Yves-Placide Assoumou Ndong</name>
<affiliation wicri:level="3"><nlm:aff id="I2">EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Picardie</region>
<settlement type="city">Amiens</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Duarte, Jorge" sort="Duarte, Jorge" uniqKey="Duarte J" first="Jorge" last="Duarte">Jorge Duarte</name>
<affiliation wicri:level="3"><nlm:aff id="I3">BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Clermont-Ferrand</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Riviere, Nathalie" sort="Riviere, Nathalie" uniqKey="Riviere N" first="Nathalie" last="Rivière">Nathalie Rivière</name>
<affiliation wicri:level="3"><nlm:aff id="I3">BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Clermont-Ferrand</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wilmer, Jeroen" sort="Wilmer, Jeroen" uniqKey="Wilmer J" first="Jeroen" last="Wilmer">Jeroen Wilmer</name>
<affiliation wicri:level="3"><nlm:aff id="I4">BIOGEMMA, domaine de Sandreau, Chemin de Panedautes, 31700 Mondonville, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>BIOGEMMA, domaine de Sandreau, Chemin de Panedautes, 31700 Mondonville</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Midi-Pyrénées</region>
<settlement type="city">Mondonville</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Van Wuytswinkel, Olivier" sort="Van Wuytswinkel, Olivier" uniqKey="Van Wuytswinkel O" first="Olivier" last="Van Wuytswinkel">Olivier Van Wuytswinkel</name>
<affiliation wicri:level="3"><nlm:aff id="I2">EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Picardie</region>
<settlement type="city">Amiens</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lucau, Anca" sort="Lucau, Anca" uniqKey="Lucau A" first="Anca" last="Lucau">Anca Lucau</name>
<affiliation wicri:level="3"><nlm:aff id="I1">Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cariou, Emmanuelle" sort="Cariou, Emmanuelle" uniqKey="Cariou E" first="Emmanuelle" last="Cariou">Emmanuelle Cariou</name>
<affiliation wicri:level="3"><nlm:aff id="I5">Institut Technique de Lin, 27170 Ecardenville La Campagne, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut Technique de Lin, 27170 Ecardenville La Campagne</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Ecardenville La Campagne</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Neutelings, Godfrey" sort="Neutelings, Godfrey" uniqKey="Neutelings G" first="Godfrey" last="Neutelings">Godfrey Neutelings</name>
<affiliation wicri:level="3"><nlm:aff id="I1">Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gutierrez, Laurent" sort="Gutierrez, Laurent" uniqKey="Gutierrez L" first="Laurent" last="Gutierrez">Laurent Gutierrez</name>
<affiliation wicri:level="3"><nlm:aff id="I6">CRRBM, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>CRRBM, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Picardie</region>
<settlement type="city">Amiens</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chabbert, Brigitte" sort="Chabbert, Brigitte" uniqKey="Chabbert B" first="Brigitte" last="Chabbert">Brigitte Chabbert</name>
<affiliation wicri:level="3"><nlm:aff id="I7">UMR- INRA, UMR614, URCA, FARE, 2 Esplanade R. Garros, CREA, BP 224, 51686 Reims, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR- INRA, UMR614, URCA, FARE, 2 Esplanade R. Garros, CREA, BP 224, 51686 Reims</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Champagne-Ardenne</region>
<settlement type="city">Reims</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Guillot, Xavier" sort="Guillot, Xavier" uniqKey="Guillot X" first="Xavier" last="Guillot">Xavier Guillot</name>
<affiliation wicri:level="1"><nlm:aff id="I8">Laboulet Semences, 80 270 Airaines, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboulet Semences, 80 270 Airaines</wicri:regionArea>
<wicri:noRegion>80 270 Airaines</wicri:noRegion>
<wicri:noRegion>80 270 Airaines</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tavernier, Reynald" sort="Tavernier, Reynald" uniqKey="Tavernier R" first="Reynald" last="Tavernier">Reynald Tavernier</name>
<affiliation wicri:level="1"><nlm:aff id="I9">LINEA, 20 Avenue Saget, 60 210 Grandvilliers, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>LINEA, 20 Avenue Saget, 60 210 Grandvilliers</wicri:regionArea>
<wicri:noRegion>60 210 Grandvilliers</wicri:noRegion>
<wicri:noRegion>60 210 Grandvilliers</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hawkins, Simon" sort="Hawkins, Simon" uniqKey="Hawkins S" first="Simon" last="Hawkins">Simon Hawkins</name>
<affiliation wicri:level="3"><nlm:aff id="I1">Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Thomasset, Brigitte" sort="Thomasset, Brigitte" uniqKey="Thomasset B" first="Brigitte" last="Thomasset">Brigitte Thomasset</name>
<affiliation wicri:level="3"><nlm:aff id="I10">UMR CNRS 6022, GEC, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR CNRS 6022, GEC, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Picardie</region>
<settlement type="city">Compiègne</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">20964859</idno>
<idno type="pmc">3091737</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091737</idno>
<idno type="RBID">PMC:3091737</idno>
<idno type="doi">10.1186/1471-2164-11-592</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">000A94</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A94</idno>
<idno type="wicri:Area/Pmc/Curation">000A94</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000A94</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001360</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001360</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:20964859</idno>
<idno type="wicri:Area/PubMed/Corpus">001F29</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001F29</idno>
<idno type="wicri:Area/PubMed/Curation">001F29</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001F29</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001E55</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001E55</idno>
<idno type="wicri:Area/Ncbi/Merge">000782</idno>
<idno type="wicri:Area/Ncbi/Curation">000782</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000782</idno>
<idno type="wicri:Area/Main/Merge">002670</idno>
<idno type="wicri:Area/Main/Curation">002645</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Development and validation of a flax (<italic>Linum usitatissimum </italic>
L.) gene expression oligo microarray</title>
<author><name sortKey="Fenart, Stephane" sort="Fenart, Stephane" uniqKey="Fenart S" first="Stéphane" last="Fenart">Stéphane Fenart</name>
<affiliation wicri:level="3"><nlm:aff id="I1">Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ndong, Yves Placide Assoumou" sort="Ndong, Yves Placide Assoumou" uniqKey="Ndong Y" first="Yves-Placide Assoumou" last="Ndong">Yves-Placide Assoumou Ndong</name>
<affiliation wicri:level="3"><nlm:aff id="I2">EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Picardie</region>
<settlement type="city">Amiens</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Duarte, Jorge" sort="Duarte, Jorge" uniqKey="Duarte J" first="Jorge" last="Duarte">Jorge Duarte</name>
<affiliation wicri:level="3"><nlm:aff id="I3">BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Clermont-Ferrand</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Riviere, Nathalie" sort="Riviere, Nathalie" uniqKey="Riviere N" first="Nathalie" last="Rivière">Nathalie Rivière</name>
<affiliation wicri:level="3"><nlm:aff id="I3">BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>BIOGEMMA, Z.I. du Brezet, 8 rue des Frères Lumières, 63028 Clermont-Ferrand cedex 2</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Clermont-Ferrand</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wilmer, Jeroen" sort="Wilmer, Jeroen" uniqKey="Wilmer J" first="Jeroen" last="Wilmer">Jeroen Wilmer</name>
<affiliation wicri:level="3"><nlm:aff id="I4">BIOGEMMA, domaine de Sandreau, Chemin de Panedautes, 31700 Mondonville, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>BIOGEMMA, domaine de Sandreau, Chemin de Panedautes, 31700 Mondonville</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Midi-Pyrénées</region>
<settlement type="city">Mondonville</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Van Wuytswinkel, Olivier" sort="Van Wuytswinkel, Olivier" uniqKey="Van Wuytswinkel O" first="Olivier" last="Van Wuytswinkel">Olivier Van Wuytswinkel</name>
<affiliation wicri:level="3"><nlm:aff id="I2">EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>EA 3900-BioPI, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Picardie</region>
<settlement type="city">Amiens</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lucau, Anca" sort="Lucau, Anca" uniqKey="Lucau A" first="Anca" last="Lucau">Anca Lucau</name>
<affiliation wicri:level="3"><nlm:aff id="I1">Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Cariou, Emmanuelle" sort="Cariou, Emmanuelle" uniqKey="Cariou E" first="Emmanuelle" last="Cariou">Emmanuelle Cariou</name>
<affiliation wicri:level="3"><nlm:aff id="I5">Institut Technique de Lin, 27170 Ecardenville La Campagne, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Institut Technique de Lin, 27170 Ecardenville La Campagne</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Normandie</region>
<region type="old region" nuts="2">Haute-Normandie</region>
<settlement type="city">Ecardenville La Campagne</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Neutelings, Godfrey" sort="Neutelings, Godfrey" uniqKey="Neutelings G" first="Godfrey" last="Neutelings">Godfrey Neutelings</name>
<affiliation wicri:level="3"><nlm:aff id="I1">Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gutierrez, Laurent" sort="Gutierrez, Laurent" uniqKey="Gutierrez L" first="Laurent" last="Gutierrez">Laurent Gutierrez</name>
<affiliation wicri:level="3"><nlm:aff id="I6">CRRBM, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>CRRBM, UFR des Sciences, UPJV, 33 rue Saint Leu, 80039 Amiens cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Picardie</region>
<settlement type="city">Amiens</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chabbert, Brigitte" sort="Chabbert, Brigitte" uniqKey="Chabbert B" first="Brigitte" last="Chabbert">Brigitte Chabbert</name>
<affiliation wicri:level="3"><nlm:aff id="I7">UMR- INRA, UMR614, URCA, FARE, 2 Esplanade R. Garros, CREA, BP 224, 51686 Reims, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR- INRA, UMR614, URCA, FARE, 2 Esplanade R. Garros, CREA, BP 224, 51686 Reims</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Champagne-Ardenne</region>
<settlement type="city">Reims</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Guillot, Xavier" sort="Guillot, Xavier" uniqKey="Guillot X" first="Xavier" last="Guillot">Xavier Guillot</name>
<affiliation wicri:level="1"><nlm:aff id="I8">Laboulet Semences, 80 270 Airaines, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Laboulet Semences, 80 270 Airaines</wicri:regionArea>
<wicri:noRegion>80 270 Airaines</wicri:noRegion>
<wicri:noRegion>80 270 Airaines</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tavernier, Reynald" sort="Tavernier, Reynald" uniqKey="Tavernier R" first="Reynald" last="Tavernier">Reynald Tavernier</name>
<affiliation wicri:level="1"><nlm:aff id="I9">LINEA, 20 Avenue Saget, 60 210 Grandvilliers, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>LINEA, 20 Avenue Saget, 60 210 Grandvilliers</wicri:regionArea>
<wicri:noRegion>60 210 Grandvilliers</wicri:noRegion>
<wicri:noRegion>60 210 Grandvilliers</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hawkins, Simon" sort="Hawkins, Simon" uniqKey="Hawkins S" first="Simon" last="Hawkins">Simon Hawkins</name>
<affiliation wicri:level="3"><nlm:aff id="I1">Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université Lille Nord de France, Lille 1 UMR INRA 1281, SADV, F- 59650 Villeneuve d'Ascq cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Nord-Pas-de-Calais</region>
<settlement type="city">Villeneuve d'Ascq</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Thomasset, Brigitte" sort="Thomasset, Brigitte" uniqKey="Thomasset B" first="Brigitte" last="Thomasset">Brigitte Thomasset</name>
<affiliation wicri:level="3"><nlm:aff id="I10">UMR CNRS 6022, GEC, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne cedex, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>UMR CNRS 6022, GEC, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Hauts-de-France</region>
<region type="old region" nuts="2">Picardie</region>
<settlement type="city">Compiègne</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC Genomics</title>
<idno type="eISSN">1471-2164</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Contig Mapping</term>
<term>Flax (genetics)</term>
<term>Flax (growth & development)</term>
<term>Gene Expression Profiling</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genes, Plant (genetics)</term>
<term>Genotype</term>
<term>Molecular Sequence Annotation</term>
<term>Oligonucleotide Array Sequence Analysis (methods)</term>
<term>Organ Specificity (genetics)</term>
<term>Plant Stems (genetics)</term>
<term>Principal Component Analysis</term>
<term>Reproducibility of Results</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Analyse de séquence d'ADN</term>
<term>Analyse en composantes principales</term>
<term>Annotation de séquence moléculaire</term>
<term>Cartographie de contigs</term>
<term>Gènes de plante (génétique)</term>
<term>Génotype</term>
<term>Lin (croissance et développement)</term>
<term>Lin (génétique)</term>
<term>RT-PCR</term>
<term>Reproductibilité des résultats</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Spécificité d'organe (génétique)</term>
<term>Séquençage par oligonucléotides en batterie ()</term>
<term>Tiges de plante (génétique)</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Lin</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Flax</term>
<term>Genes, Plant</term>
<term>Organ Specificity</term>
<term>Plant Stems</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Flax</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Gènes de plante</term>
<term>Lin</term>
<term>Spécificité d'organe</term>
<term>Tiges de plante</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Oligonucleotide Array Sequence Analysis</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Contig Mapping</term>
<term>Gene Expression Profiling</term>
<term>Gene Expression Regulation, Developmental</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genotype</term>
<term>Molecular Sequence Annotation</term>
<term>Principal Component Analysis</term>
<term>Reproducibility of Results</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Analyse de séquence d'ADN</term>
<term>Analyse en composantes principales</term>
<term>Annotation de séquence moléculaire</term>
<term>Cartographie de contigs</term>
<term>Génotype</term>
<term>RT-PCR</term>
<term>Reproductibilité des résultats</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Séquençage par oligonucléotides en batterie</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Flax (<italic>Linum usitatissimum </italic>
L.) has been cultivated for around 9,000 years and is therefore one of the oldest cultivated species. Today, flax is still grown for its oil (oil-flax or linseed cultivars) and its cellulose-rich fibres (fibre-flax cultivars) used for high-value linen garments and composite materials. Despite the wide industrial use of flax-derived products, and our actual understanding of the regulation of both wood fibre production and oil biosynthesis more information must be acquired in both domains. Recent advances in genomics are now providing opportunities to improve our fundamental knowledge of these complex processes. In this paper we report the development and validation of a high-density oligo microarray platform dedicated to gene expression analyses in flax.</p>
</sec>
<sec><title>Results</title>
<p>Nine different RNA samples obtained from flax inner- and outer-stems, seeds, leaves and roots were used to generate a collection of 1,066,481 ESTs by massive parallel pyrosequencing. Sequences were assembled into 59,626 unigenes and 48,021 sequences were selected for oligo design and high-density microarray (Nimblegen 385K) fabrication with eight, non-overlapping 25-mers oligos per unigene. 18 independent experiments were used to evaluate the hybridization quality, precision, specificity and accuracy and all results confirmed the high technical quality of our microarray platform. Cross-validation of microarray data was carried out using quantitative qRT-PCR. Nine target genes were selected on the basis of microarray results and reflected the whole range of fold change (both up-regulated and down-regulated genes in different samples). A statistically significant positive correlation was obtained comparing expression levels for each target gene across all biological replicates both in qRT-PCR and microarray results. Further experiments illustrated the capacity of our arrays to detect differential gene expression in a variety of flax tissues as well as between two contrasted flax varieties.</p>
</sec>
<sec><title>Conclusion</title>
<p>All results suggest that our high-density flax oligo-microarray platform can be used as a very sensitive tool for analyzing gene expression in a large variety of tissues as well as in different cultivars. Moreover, this highly reliable platform can also be used for the quantification of mRNA transcriptional profiling in different flax tissues.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Deyholos, Mk" uniqKey="Deyholos M">MK Deyholos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vaisey Genser, M" uniqKey="Vaisey Genser M">M Vaisey-Genser</name>
</author>
<author><name sortKey="Morris, Dh" uniqKey="Morris D">DH Morris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Baley, C" uniqKey="Baley C">C Baley</name>
</author>
<author><name sortKey="Perrot, Y" uniqKey="Perrot Y">Y Perrot</name>
</author>
<author><name sortKey="Busnel, F" uniqKey="Busnel F">F Busnel</name>
</author>
<author><name sortKey="Guezenoc, H" uniqKey="Guezenoc H">H Guezenoc</name>
</author>
<author><name sortKey="Davies, P" uniqKey="Davies P">P Davies</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kym L Inen, Hr" uniqKey="Kym L Inen H">HR Kymäläinen</name>
</author>
<author><name sortKey="Sjoberga, Am" uniqKey="Sjoberga A">AM Sjöberga</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Farmer, C" uniqKey="Farmer C">C Farmer</name>
</author>
<author><name sortKey="Petit, Hv" uniqKey="Petit H">HV Petit</name>
</author>
<author><name sortKey="Weiler, H" uniqKey="Weiler H">H Weiler</name>
</author>
<author><name sortKey="Capuco, Av" uniqKey="Capuco A">AV Capuco</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dodin, S" uniqKey="Dodin S">S Dodin</name>
</author>
<author><name sortKey="Cunnane, Sc" uniqKey="Cunnane S">SC Cunnane</name>
</author>
<author><name sortKey="Masse, B" uniqKey="Masse B">B Masse</name>
</author>
<author><name sortKey="Lemay, A" uniqKey="Lemay A">A Lemay</name>
</author>
<author><name sortKey="Jacques, H" uniqKey="Jacques H">H Jacques</name>
</author>
<author><name sortKey="Asselin, G" uniqKey="Asselin G">G Asselin</name>
</author>
<author><name sortKey="Tremblay Mercier, J" uniqKey="Tremblay Mercier J">J Tremblay-Mercier</name>
</author>
<author><name sortKey="Marc, I" uniqKey="Marc I">I Marc</name>
</author>
<author><name sortKey="Lamarche, B" uniqKey="Lamarche B">B Lamarche</name>
</author>
<author><name sortKey="Legare, F" uniqKey="Legare F">F Legare</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Felmlee, Ma" uniqKey="Felmlee M">MA Felmlee</name>
</author>
<author><name sortKey="Woo, G" uniqKey="Woo G">G Woo</name>
</author>
<author><name sortKey="Simko, E" uniqKey="Simko E">E Simko</name>
</author>
<author><name sortKey="Krol, Es" uniqKey="Krol E">ES Krol</name>
</author>
<author><name sortKey="Muir, Ad" uniqKey="Muir A">AD Muir</name>
</author>
<author><name sortKey="Alcorn, J" uniqKey="Alcorn J">J Alcorn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name>
</author>
<author><name sortKey="Soucek, Md" uniqKey="Soucek M">MD Soucek</name>
</author>
<author><name sortKey="Simonsick, Wj" uniqKey="Simonsick W">WJ Simonsick</name>
</author>
<author><name sortKey="Celikay, Rw" uniqKey="Celikay R">RW Celikay</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hall, C" uniqKey="Hall C">C Hall</name>
</author>
<author><name sortKey="Tulbek, Mc" uniqKey="Tulbek M">MC Tulbek</name>
</author>
<author><name sortKey="Xu, Y" uniqKey="Xu Y">Y Xu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Day, A" uniqKey="Day A">A Day</name>
</author>
<author><name sortKey="Neutelings, G" uniqKey="Neutelings G">G Neutelings</name>
</author>
<author><name sortKey="Nolin, F" uniqKey="Nolin F">F Nolin</name>
</author>
<author><name sortKey="Grec, S" uniqKey="Grec S">S Grec</name>
</author>
<author><name sortKey="Habrant, A" uniqKey="Habrant A">A Habrant</name>
</author>
<author><name sortKey="Cronier, D" uniqKey="Cronier D">D Cronier</name>
</author>
<author><name sortKey="Maher, B" uniqKey="Maher B">B Maher</name>
</author>
<author><name sortKey="Rolando, C" uniqKey="Rolando C">C Rolando</name>
</author>
<author><name sortKey="David, H" uniqKey="David H">H David</name>
</author>
<author><name sortKey="Chabbert, B" uniqKey="Chabbert B">B Chabbert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gutierrez, L" uniqKey="Gutierrez L">L Gutierrez</name>
</author>
<author><name sortKey="Conejero, G" uniqKey="Conejero G">G Conejero</name>
</author>
<author><name sortKey="Castelain, M" uniqKey="Castelain M">M Castelain</name>
</author>
<author><name sortKey="Guenin, S" uniqKey="Guenin S">S Guenin</name>
</author>
<author><name sortKey="Verdeil, Jl" uniqKey="Verdeil J">JL Verdeil</name>
</author>
<author><name sortKey="Thomasset, B" uniqKey="Thomasset B">B Thomasset</name>
</author>
<author><name sortKey="Van Wuytswinkel, O" uniqKey="Van Wuytswinkel O">O Van Wuytswinkel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rao, S" uniqKey="Rao S">S Rao</name>
</author>
<author><name sortKey="Abdel Reheem, M" uniqKey="Abdel Reheem M">M Abdel-Reheem</name>
</author>
<author><name sortKey="Bhella, R" uniqKey="Bhella R">R Bhella</name>
</author>
<author><name sortKey="Mccracken, C" uniqKey="Mccracken C">C McCracken</name>
</author>
<author><name sortKey="Hildebrand, D" uniqKey="Hildebrand D">D Hildebrand</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bennett, Md" uniqKey="Bennett M">MD Bennett</name>
</author>
<author><name sortKey="Leitch, Ij" uniqKey="Leitch I">IJ Leitch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Day, A" uniqKey="Day A">A Day</name>
</author>
<author><name sortKey="Addi, M" uniqKey="Addi M">M Addi</name>
</author>
<author><name sortKey="Kim, W" uniqKey="Kim W">W Kim</name>
</author>
<author><name sortKey="David, H" uniqKey="David H">H David</name>
</author>
<author><name sortKey="Bert, F" uniqKey="Bert F">F Bert</name>
</author>
<author><name sortKey="Mesnage, P" uniqKey="Mesnage P">P Mesnage</name>
</author>
<author><name sortKey="Rolando, C" uniqKey="Rolando C">C Rolando</name>
</author>
<author><name sortKey="Chabbert, B" uniqKey="Chabbert B">B Chabbert</name>
</author>
<author><name sortKey="Neutelings, G" uniqKey="Neutelings G">G Neutelings</name>
</author>
<author><name sortKey="Hawkins, S" uniqKey="Hawkins S">S Hawkins</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Pauw, Ma" uniqKey="De Pauw M">MA De Pauw</name>
</author>
<author><name sortKey="Vidmar, Jj" uniqKey="Vidmar J">JJ Vidmar</name>
</author>
<author><name sortKey="Collins, J" uniqKey="Collins J">J Collins</name>
</author>
<author><name sortKey="Bennett, Ra" uniqKey="Bennett R">RA Bennett</name>
</author>
<author><name sortKey="Deyholos, Mk" uniqKey="Deyholos M">MK Deyholos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hotte, Ns" uniqKey="Hotte N">NS Hotte</name>
</author>
<author><name sortKey="Deyholos, Mk" uniqKey="Deyholos M">MK Deyholos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roach, Mj" uniqKey="Roach M">MJ Roach</name>
</author>
<author><name sortKey="Deyholos, Mk" uniqKey="Deyholos M">MK Deyholos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roach, Mj" uniqKey="Roach M">MJ Roach</name>
</author>
<author><name sortKey="Deyholos, Mk" uniqKey="Deyholos M">MK Deyholos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moreau, Y" uniqKey="Moreau Y">Y Moreau</name>
</author>
<author><name sortKey="Aerts, S" uniqKey="Aerts S">S Aerts</name>
</author>
<author><name sortKey="De Moor, B" uniqKey="De Moor B">B De Moor</name>
</author>
<author><name sortKey="De Strooper, B" uniqKey="De Strooper B">B De Strooper</name>
</author>
<author><name sortKey="Dabrowski, M" uniqKey="Dabrowski M">M Dabrowski</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nimgaonkar, A" uniqKey="Nimgaonkar A">A Nimgaonkar</name>
</author>
<author><name sortKey="Sanoudou, D" uniqKey="Sanoudou D">D Sanoudou</name>
</author>
<author><name sortKey="Butte, Aj" uniqKey="Butte A">AJ Butte</name>
</author>
<author><name sortKey="Haslett, Jn" uniqKey="Haslett J">JN Haslett</name>
</author>
<author><name sortKey="Kunkel, Lm" uniqKey="Kunkel L">LM Kunkel</name>
</author>
<author><name sortKey="Beggs, Ah" uniqKey="Beggs A">AH Beggs</name>
</author>
<author><name sortKey="Kohane, Is" uniqKey="Kohane I">IS Kohane</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hardiman, G" uniqKey="Hardiman G">G Hardiman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y Wang</name>
</author>
<author><name sortKey="Barbacioru, C" uniqKey="Barbacioru C">C Barbacioru</name>
</author>
<author><name sortKey="Hyland, F" uniqKey="Hyland F">F Hyland</name>
</author>
<author><name sortKey="Xiao, W" uniqKey="Xiao W">W Xiao</name>
</author>
<author><name sortKey="Hunkapiller, Kl" uniqKey="Hunkapiller K">KL Hunkapiller</name>
</author>
<author><name sortKey="Blake, J" uniqKey="Blake J">J Blake</name>
</author>
<author><name sortKey="Chan, F" uniqKey="Chan F">F Chan</name>
</author>
<author><name sortKey="Gonzalez, C" uniqKey="Gonzalez C">C Gonzalez</name>
</author>
<author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name>
</author>
<author><name sortKey="Samaha, Rr" uniqKey="Samaha R">RR Samaha</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buick, R" uniqKey="Buick R">R Buick</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Campbell, Na" uniqKey="Campbell N">NA Campbell</name>
</author>
<author><name sortKey="Williamson, B" uniqKey="Williamson B">B Williamson</name>
</author>
<author><name sortKey="Heyden, Rj" uniqKey="Heyden R">RJ Heyden</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Douglas, Se" uniqKey="Douglas S">SE Douglas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gould, Sb" uniqKey="Gould S">SB Gould</name>
</author>
<author><name sortKey="Waller, Rf" uniqKey="Waller R">RF Waller</name>
</author>
<author><name sortKey="Mcfadden, Gi" uniqKey="Mcfadden G">GI McFadden</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Nealson, Kh" uniqKey="Nealson K">KH Nealson</name>
</author>
<author><name sortKey="Conrad, Pg" uniqKey="Conrad P">PG Conrad</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Raven, Ph" uniqKey="Raven P">PH Raven</name>
</author>
<author><name sortKey="Evert, Rf" uniqKey="Evert R">RF Evert</name>
</author>
<author><name sortKey="Eichhorn, Se" uniqKey="Eichhorn S">SE Eichhorn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reyes Prieto, A" uniqKey="Reyes Prieto A">A Reyes-Prieto</name>
</author>
<author><name sortKey="Weber, Ap" uniqKey="Weber A">AP Weber</name>
</author>
<author><name sortKey="Bhattacharya, D" uniqKey="Bhattacharya D">D Bhattacharya</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rodriguez Ezpeleta, N" uniqKey="Rodriguez Ezpeleta N">N Rodriguez-Ezpeleta</name>
</author>
<author><name sortKey="Brinkmann, H" uniqKey="Brinkmann H">H Brinkmann</name>
</author>
<author><name sortKey="Burey, Sc" uniqKey="Burey S">SC Burey</name>
</author>
<author><name sortKey="Roure, B" uniqKey="Roure B">B Roure</name>
</author>
<author><name sortKey="Burger, G" uniqKey="Burger G">G Burger</name>
</author>
<author><name sortKey="Loffelhardt, W" uniqKey="Loffelhardt W">W Loffelhardt</name>
</author>
<author><name sortKey="Bohnert, Hj" uniqKey="Bohnert H">HJ Bohnert</name>
</author>
<author><name sortKey="Philippe, H" uniqKey="Philippe H">H Philippe</name>
</author>
<author><name sortKey="Lang, Bf" uniqKey="Lang B">BF Lang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Smith, Al" uniqKey="Smith A">AL Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bengough, Ag" uniqKey="Bengough A">AG Bengough</name>
</author>
<author><name sortKey="Bransby, Mf" uniqKey="Bransby M">MF Bransby</name>
</author>
<author><name sortKey="Hans, J" uniqKey="Hans J">J Hans</name>
</author>
<author><name sortKey="Mckenna, Sj" uniqKey="Mckenna S">SJ McKenna</name>
</author>
<author><name sortKey="Roberts, Tj" uniqKey="Roberts T">TJ Roberts</name>
</author>
<author><name sortKey="Valentine, Ta" uniqKey="Valentine T">TA Valentine</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Okubara, Pa" uniqKey="Okubara P">PA Okubara</name>
</author>
<author><name sortKey="Paulitz, Tc" uniqKey="Paulitz T">TC Paulitz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Day, A" uniqKey="Day A">A Day</name>
</author>
<author><name sortKey="Ruel, K" uniqKey="Ruel K">K Ruel</name>
</author>
<author><name sortKey="Neutelings, G" uniqKey="Neutelings G">G Neutelings</name>
</author>
<author><name sortKey="Cronier, D" uniqKey="Cronier D">D Cronier</name>
</author>
<author><name sortKey="David, H" uniqKey="David H">H David</name>
</author>
<author><name sortKey="Hawkins, S" uniqKey="Hawkins S">S Hawkins</name>
</author>
<author><name sortKey="Chabbert, B" uniqKey="Chabbert B">B Chabbert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dupree, P" uniqKey="Dupree P">P Dupree</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Troufflard, S" uniqKey="Troufflard S">S Troufflard</name>
</author>
<author><name sortKey="Roscher, A" uniqKey="Roscher A">A Roscher</name>
</author>
<author><name sortKey="Thomasset, B" uniqKey="Thomasset B">B Thomasset</name>
</author>
<author><name sortKey="Barbotin, Jn" uniqKey="Barbotin J">JN Barbotin</name>
</author>
<author><name sortKey="Rawsthorne, S" uniqKey="Rawsthorne S">S Rawsthorne</name>
</author>
<author><name sortKey="Portais, Jc" uniqKey="Portais J">JC Portais</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mellerowicz, Ej" uniqKey="Mellerowicz E">EJ Mellerowicz</name>
</author>
<author><name sortKey="Sundberg, B" uniqKey="Sundberg B">B Sundberg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Larfarguette, F" uniqKey="Larfarguette F">F Larfarguette</name>
</author>
<author><name sortKey="Leple, Jc" uniqKey="Leple J">JC Leplé</name>
</author>
<author><name sortKey="Dejardin, A" uniqKey="Dejardin A">A Déjardin</name>
</author>
<author><name sortKey="Laurens, F" uniqKey="Laurens F">F Laurens</name>
</author>
<author><name sortKey="Costa, G" uniqKey="Costa G">G Costa</name>
</author>
<author><name sortKey="Lesage Descauses, Mc" uniqKey="Lesage Descauses M">MC Lesage-Descauses</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yeats, Th" uniqKey="Yeats T">TH Yeats</name>
</author>
<author><name sortKey="Rose, Jkc" uniqKey="Rose J">JKC Rose</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gorshkova, Ta" uniqKey="Gorshkova T">TA Gorshkova</name>
</author>
<author><name sortKey="Sal Nikov, Vv" uniqKey="Sal Nikov V">VV Sal'nikov</name>
</author>
<author><name sortKey="Chemikosova, Sb" uniqKey="Chemikosova S">SB Chemikosova</name>
</author>
<author><name sortKey="Ageeva, Mv" uniqKey="Ageeva M">MV Ageeva</name>
</author>
<author><name sortKey="Pavlencheva, Nv" uniqKey="Pavlencheva N">NV Pavlencheva</name>
</author>
<author><name sortKey="Van Dam, Jeg" uniqKey="Van Dam J">JEG van Dam</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="His, I" uniqKey="His I">I His</name>
</author>
<author><name sortKey="Andeme Onzighi, C" uniqKey="Andeme Onzighi C">C Andème-Onzighi</name>
</author>
<author><name sortKey="Morvan, C" uniqKey="Morvan C">C Morvan</name>
</author>
<author><name sortKey="Driouich, A" uniqKey="Driouich A">A Driouich</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vromans, J" uniqKey="Vromans J">J Vromans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Morvan, C" uniqKey="Morvan C">C Morvan</name>
</author>
<author><name sortKey="Andeme Onzighi, C" uniqKey="Andeme Onzighi C">C Andème-Onzighi</name>
</author>
<author><name sortKey="Girault, R" uniqKey="Girault R">R Girault</name>
</author>
<author><name sortKey="Himmelsbach, Ds" uniqKey="Himmelsbach D">DS Himmelsbach</name>
</author>
<author><name sortKey="Driouich, A" uniqKey="Driouich A">A Driouich</name>
</author>
<author><name sortKey="Akin, De" uniqKey="Akin D">DE Akin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tang, Gq" uniqKey="Tang G">GQ Tang</name>
</author>
<author><name sortKey="Luscher, M" uniqKey="Luscher M">M Luscher</name>
</author>
<author><name sortKey="Sturm, A" uniqKey="Sturm A">A Sturm</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gorshkova, T" uniqKey="Gorshkova T">T Gorshkova</name>
</author>
<author><name sortKey="Gurjanov, O" uniqKey="Gurjanov O">O Gurjanov</name>
</author>
<author><name sortKey="Mikshina, P" uniqKey="Mikshina P">P Mikshina</name>
</author>
<author><name sortKey="Ibragimova, N" uniqKey="Ibragimova N">N Ibragimova</name>
</author>
<author><name sortKey="Mokshina, N" uniqKey="Mokshina N">N Mokshina</name>
</author>
<author><name sortKey="Salnikov, V" uniqKey="Salnikov V">V Salnikov</name>
</author>
<author><name sortKey="Ageeva, M" uniqKey="Ageeva M">M Ageeva</name>
</author>
<author><name sortKey="Amenitskii, S" uniqKey="Amenitskii S">S Amenitskii</name>
</author>
<author><name sortKey="Chernova, T" uniqKey="Chernova T">T Chernova</name>
</author>
<author><name sortKey="Chemikosova, S" uniqKey="Chemikosova S">S Chemikosova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hobson, N" uniqKey="Hobson N">N Hobson</name>
</author>
<author><name sortKey="Roach, M" uniqKey="Roach M">M Roach</name>
</author>
<author><name sortKey="Deyholos, M" uniqKey="Deyholos M">M Deyholos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaku, T" uniqKey="Kaku T">T Kaku</name>
</author>
<author><name sortKey="Serada, S" uniqKey="Serada S">S Serada</name>
</author>
<author><name sortKey="Baba, Ki" uniqKey="Baba K">Ki Baba</name>
</author>
<author><name sortKey="Tanaka, F" uniqKey="Tanaka F">F Tanaka</name>
</author>
<author><name sortKey="Hayashi, T" uniqKey="Hayashi T">T Hayashi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Woltering, Ej" uniqKey="Woltering E">EJ Woltering</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name>
</author>
<author><name sortKey="Xu, Q" uniqKey="Xu Q">Q Xu</name>
</author>
<author><name sortKey="Xing, D" uniqKey="Xing D">D Xing</name>
</author>
<author><name sortKey="Gao, C" uniqKey="Gao C">C Gao</name>
</author>
<author><name sortKey="Xiong, H" uniqKey="Xiong H">H Xiong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Manosalva, Pm" uniqKey="Manosalva P">PM Manosalva</name>
</author>
<author><name sortKey="Davidson, Rm" uniqKey="Davidson R">RM Davidson</name>
</author>
<author><name sortKey="Liu, B" uniqKey="Liu B">B Liu</name>
</author>
<author><name sortKey="Zhu, X" uniqKey="Zhu X">X Zhu</name>
</author>
<author><name sortKey="Hulbert, Sh" uniqKey="Hulbert S">SH Hulbert</name>
</author>
<author><name sortKey="Leung, H" uniqKey="Leung H">H Leung</name>
</author>
<author><name sortKey="Leach, Je" uniqKey="Leach J">JE Leach</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Islam, R" uniqKey="Islam R">R Islam</name>
</author>
<author><name sortKey="Sheperd, Kw" uniqKey="Sheperd K">KW Sheperd</name>
</author>
<author><name sortKey="Mayo, Gme" uniqKey="Mayo G">GME Mayo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gehrig, Hh" uniqKey="Gehrig H">HH Gehrig</name>
</author>
<author><name sortKey="Winter, K" uniqKey="Winter K">K Winter</name>
</author>
<author><name sortKey="Cushman, Jc" uniqKey="Cushman J">JC Cushman</name>
</author>
<author><name sortKey="Borland, Am" uniqKey="Borland A">AM Borland</name>
</author>
<author><name sortKey="Taybi, T" uniqKey="Taybi T">T Taybi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Verwoerd, Tc" uniqKey="Verwoerd T">TC Verwoerd</name>
</author>
<author><name sortKey="Dekker, Bm" uniqKey="Dekker B">BM Dekker</name>
</author>
<author><name sortKey="Hoekema, A" uniqKey="Hoekema A">A Hoekema</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bolstad, Bm" uniqKey="Bolstad B">BM Bolstad</name>
</author>
<author><name sortKey="Irizarry, Ra" uniqKey="Irizarry R">RA Irizarry</name>
</author>
<author><name sortKey="Astrand, M" uniqKey="Astrand M">M Astrand</name>
</author>
<author><name sortKey="Speed, Tp" uniqKey="Speed T">TP Speed</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Irizarry, Ra" uniqKey="Irizarry R">RA Irizarry</name>
</author>
<author><name sortKey="Hobbs, B" uniqKey="Hobbs B">B Hobbs</name>
</author>
<author><name sortKey="Collin, F" uniqKey="Collin F">F Collin</name>
</author>
<author><name sortKey="Beazer Barclay, Yd" uniqKey="Beazer Barclay Y">YD Beazer-Barclay</name>
</author>
<author><name sortKey="Antonellis, Kj" uniqKey="Antonellis K">KJ Antonellis</name>
</author>
<author><name sortKey="Scherf, U" uniqKey="Scherf U">U Scherf</name>
</author>
<author><name sortKey="Speed, Tp" uniqKey="Speed T">TP Speed</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tusher, Vg" uniqKey="Tusher V">VG Tusher</name>
</author>
<author><name sortKey="Tibshirani, R" uniqKey="Tibshirani R">R Tibshirani</name>
</author>
<author><name sortKey="Chu, G" uniqKey="Chu G">G Chu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dysvik, B" uniqKey="Dysvik B">B Dysvik</name>
</author>
<author><name sortKey="Jonassen, I" uniqKey="Jonassen I">I Jonassen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rieu, I" uniqKey="Rieu I">I Rieu</name>
</author>
<author><name sortKey="Powers, Sj" uniqKey="Powers S">SJ Powers</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Larionov, A" uniqKey="Larionov A">A Larionov</name>
</author>
<author><name sortKey="Krause, A" uniqKey="Krause A">A Krause</name>
</author>
<author><name sortKey="Miller, W" uniqKey="Miller W">W Miller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gutierrez, L" uniqKey="Gutierrez L">L Gutierrez</name>
</author>
<author><name sortKey="Mauriat, M" uniqKey="Mauriat M">M Mauriat</name>
</author>
<author><name sortKey="Pelloux, J" uniqKey="Pelloux J">J Pelloux</name>
</author>
<author><name sortKey="Bellini, C" uniqKey="Bellini C">C Bellini</name>
</author>
<author><name sortKey="Van Wuytswinkel, O" uniqKey="Van Wuytswinkel O">O Van Wuytswinkel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vandesompele, J" uniqKey="Vandesompele J">J Vandesompele</name>
</author>
<author><name sortKey="De Preter, K" uniqKey="De Preter K">K De Preter</name>
</author>
<author><name sortKey="Pattyn, F" uniqKey="Pattyn F">F Pattyn</name>
</author>
<author><name sortKey="Poppe, B" uniqKey="Poppe B">B Poppe</name>
</author>
<author><name sortKey="Van Roy, N" uniqKey="Van Roy N">N Van Roy</name>
</author>
<author><name sortKey="De Paepe, A" uniqKey="De Paepe A">A De Paepe</name>
</author>
<author><name sortKey="Speleman, F" uniqKey="Speleman F">F Speleman</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002645 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002645 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Curation |type= RBID |clé= PMC:3091737 |texte= Development and validation of a flax (Linum usitatissimum L.) gene expression oligo microarray }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:20964859" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |