Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation.
Identifieur interne : 002199 ( Main/Curation ); précédent : 002198; suivant : 002200Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation.
Auteurs : Antonio Fernández-Pevida [Espagne] ; Olga Rodríguez-Galán ; Antonio Díaz-Quintana ; Dieter Kressler ; Jesús De La CruzSource :
- The Journal of biological chemistry [ 1083-351X ] ; 2012.
Descripteurs français
- KwdFr :
- ARN ribosomique (génétique), ARN ribosomique (métabolisme), Conformation des protéines, Cytoplasme (métabolisme), Division cellulaire (), Division cellulaire (génétique), Espaceur de l'ADN ribosomique (génétique), Indènes (pharmacologie), Inhibiteurs de la synthèse protéique (pharmacologie), Maturation post-transcriptionnelle des ARN, Microscopie de fluorescence, Modèles moléculaires, Mutation, Noyau de la cellule (métabolisme), Protéines de Saccharomyces cerevisiae (), Protéines de Saccharomyces cerevisiae (génétique), Protéines de Saccharomyces cerevisiae (métabolisme), Protéines de liaison à l'ARN (génétique), Protéines de liaison à l'ARN (métabolisme), Protéines ribosomiques (), Protéines ribosomiques (génétique), Protéines ribosomiques (métabolisme), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Précurseurs des ARN (génétique), Précurseurs des ARN (métabolisme), Ribosomes (génétique), Ribosomes (métabolisme), Saccharomyces cerevisiae (croissance et développement), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Technique de Western, Transport de protéines.
- MESH :
- croissance et développement : Saccharomyces cerevisiae.
- génétique : ARN ribosomique, Division cellulaire, Espaceur de l'ADN ribosomique, Protéines de Saccharomyces cerevisiae, Protéines de liaison à l'ARN, Protéines ribosomiques, Protéines à fluorescence verte, Précurseurs des ARN, Ribosomes, Saccharomyces cerevisiae.
- métabolisme : ARN ribosomique, Cytoplasme, Noyau de la cellule, Protéines de Saccharomyces cerevisiae, Protéines de liaison à l'ARN, Protéines ribosomiques, Protéines à fluorescence verte, Précurseurs des ARN, Ribosomes, Saccharomyces cerevisiae.
- pharmacologie : Indènes, Inhibiteurs de la synthèse protéique.
- Conformation des protéines, Division cellulaire, Maturation post-transcriptionnelle des ARN, Microscopie de fluorescence, Modèles moléculaires, Mutation, Protéines de Saccharomyces cerevisiae, Protéines ribosomiques, Technique de Western, Transport de protéines.
English descriptors
- KwdEn :
- Blotting, Western, Cell Division (drug effects), Cell Division (genetics), Cell Nucleus (metabolism), Cytoplasm (metabolism), DNA, Ribosomal Spacer (genetics), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Indenes (pharmacology), Microscopy, Fluorescence, Models, Molecular, Mutation, Protein Conformation, Protein Synthesis Inhibitors (pharmacology), Protein Transport, RNA Precursors (genetics), RNA Precursors (metabolism), RNA Processing, Post-Transcriptional, RNA, Ribosomal (genetics), RNA, Ribosomal (metabolism), RNA-Binding Proteins (genetics), RNA-Binding Proteins (metabolism), Ribosomal Proteins (chemistry), Ribosomal Proteins (genetics), Ribosomal Proteins (metabolism), Ribosomes (genetics), Ribosomes (metabolism), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (growth & development), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (chemistry), Saccharomyces cerevisiae Proteins (genetics), Saccharomyces cerevisiae Proteins (metabolism).
- MESH :
- chemical , chemistry : Ribosomal Proteins, Saccharomyces cerevisiae Proteins.
- chemical , genetics : DNA, Ribosomal Spacer, Green Fluorescent Proteins, RNA Precursors, RNA, Ribosomal, RNA-Binding Proteins, Ribosomal Proteins, Saccharomyces cerevisiae Proteins.
- drug effects : Cell Division.
- genetics : Cell Division, Ribosomes, Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Cell Nucleus, Cytoplasm, Green Fluorescent Proteins, RNA Precursors, RNA, Ribosomal, RNA-Binding Proteins, Ribosomal Proteins, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins.
- chemical , pharmacology : Indenes, Protein Synthesis Inhibitors.
- Blotting, Western, Microscopy, Fluorescence, Models, Molecular, Mutation, Protein Conformation, Protein Transport, RNA Processing, Post-Transcriptional.
Abstract
Most ribosomal proteins play important roles in ribosome biogenesis and function. Here, we have examined the contribution of the essential ribosomal protein L40 in these processes in the yeast Saccharomyces cerevisiae. Deletion of either the RPL40A or RPL40B gene and in vivo depletion of L40 impair 60 S ribosomal subunit biogenesis. Polysome profile analyses reveal the accumulation of half-mers and a moderate reduction in free 60 S ribosomal subunits. Pulse-chase, Northern blotting, and primer extension analyses in the L40-depleted strain clearly indicate that L40 is not strictly required for the precursor rRNA (pre-rRNA) processing reactions but contributes to optimal 27 SB pre-rRNA maturation. Moreover, depletion of L40 hinders the nucleo-cytoplasmic export of pre-60 S ribosomal particles. Importantly, all these defects most likely appear as the direct consequence of impaired Nmd3 and Rlp24 release from cytoplasmic pre-60 S ribosomal subunits and their inefficient recycling back into the nucle(ol)us. In agreement, we show that hemagglutinin epitope-tagged L40A assembles in the cytoplasm into almost mature pre-60 S ribosomal particles. Finally, we have identified that the hemagglutinin epitope-tagged L40A confers resistance to sordarin, a translation inhibitor that impairs the function of eukaryotic elongation factor 2, whereas the rpl40a and rpl40b null mutants are hypersensitive to this antibiotic. We conclude that L40 is assembled at a very late stage into pre-60 S ribosomal subunits and that its incorporation into 60 S ribosomal subunits is a prerequisite for subunit joining and may ensure proper functioning of the translocation process.
DOI: 10.1074/jbc.M112.400564
PubMed: 22995916
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001D47
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001D47
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001C24
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000996
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000996
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000996
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :002224
Links to Exploration step
pubmed:22995916Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation.</title>
<author><name sortKey="Fernandez Pevida, Antonio" sort="Fernandez Pevida, Antonio" uniqKey="Fernandez Pevida A" first="Antonio" last="Fernández-Pevida">Antonio Fernández-Pevida</name>
<affiliation wicri:level="2"><nlm:affiliation>Departamento de Genética, Universidad de Sevilla, E-41012 Sevilla, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Departamento de Genética, Universidad de Sevilla, E-41012 Sevilla</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Andalousie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rodriguez Galan, Olga" sort="Rodriguez Galan, Olga" uniqKey="Rodriguez Galan O" first="Olga" last="Rodríguez-Galán">Olga Rodríguez-Galán</name>
</author>
<author><name sortKey="Diaz Quintana, Antonio" sort="Diaz Quintana, Antonio" uniqKey="Diaz Quintana A" first="Antonio" last="Díaz-Quintana">Antonio Díaz-Quintana</name>
</author>
<author><name sortKey="Kressler, Dieter" sort="Kressler, Dieter" uniqKey="Kressler D" first="Dieter" last="Kressler">Dieter Kressler</name>
</author>
<author><name sortKey="De La Cruz, Jesus" sort="De La Cruz, Jesus" uniqKey="De La Cruz J" first="Jesús" last="De La Cruz">Jesús De La Cruz</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2012">2012</date>
<idno type="RBID">pubmed:22995916</idno>
<idno type="pmid">22995916</idno>
<idno type="doi">10.1074/jbc.M112.400564</idno>
<idno type="wicri:Area/PubMed/Corpus">001D47</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001D47</idno>
<idno type="wicri:Area/PubMed/Curation">001D47</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001D47</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001C24</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001C24</idno>
<idno type="wicri:Area/Ncbi/Merge">000996</idno>
<idno type="wicri:Area/Ncbi/Curation">000996</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000996</idno>
<idno type="wicri:Area/Main/Merge">002224</idno>
<idno type="wicri:Area/Main/Curation">002199</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation.</title>
<author><name sortKey="Fernandez Pevida, Antonio" sort="Fernandez Pevida, Antonio" uniqKey="Fernandez Pevida A" first="Antonio" last="Fernández-Pevida">Antonio Fernández-Pevida</name>
<affiliation wicri:level="2"><nlm:affiliation>Departamento de Genética, Universidad de Sevilla, E-41012 Sevilla, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Departamento de Genética, Universidad de Sevilla, E-41012 Sevilla</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Andalousie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Rodriguez Galan, Olga" sort="Rodriguez Galan, Olga" uniqKey="Rodriguez Galan O" first="Olga" last="Rodríguez-Galán">Olga Rodríguez-Galán</name>
</author>
<author><name sortKey="Diaz Quintana, Antonio" sort="Diaz Quintana, Antonio" uniqKey="Diaz Quintana A" first="Antonio" last="Díaz-Quintana">Antonio Díaz-Quintana</name>
</author>
<author><name sortKey="Kressler, Dieter" sort="Kressler, Dieter" uniqKey="Kressler D" first="Dieter" last="Kressler">Dieter Kressler</name>
</author>
<author><name sortKey="De La Cruz, Jesus" sort="De La Cruz, Jesus" uniqKey="De La Cruz J" first="Jesús" last="De La Cruz">Jesús De La Cruz</name>
</author>
</analytic>
<series><title level="j">The Journal of biological chemistry</title>
<idno type="eISSN">1083-351X</idno>
<imprint><date when="2012" type="published">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Western</term>
<term>Cell Division (drug effects)</term>
<term>Cell Division (genetics)</term>
<term>Cell Nucleus (metabolism)</term>
<term>Cytoplasm (metabolism)</term>
<term>DNA, Ribosomal Spacer (genetics)</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Indenes (pharmacology)</term>
<term>Microscopy, Fluorescence</term>
<term>Models, Molecular</term>
<term>Mutation</term>
<term>Protein Conformation</term>
<term>Protein Synthesis Inhibitors (pharmacology)</term>
<term>Protein Transport</term>
<term>RNA Precursors (genetics)</term>
<term>RNA Precursors (metabolism)</term>
<term>RNA Processing, Post-Transcriptional</term>
<term>RNA, Ribosomal (genetics)</term>
<term>RNA, Ribosomal (metabolism)</term>
<term>RNA-Binding Proteins (genetics)</term>
<term>RNA-Binding Proteins (metabolism)</term>
<term>Ribosomal Proteins (chemistry)</term>
<term>Ribosomal Proteins (genetics)</term>
<term>Ribosomal Proteins (metabolism)</term>
<term>Ribosomes (genetics)</term>
<term>Ribosomes (metabolism)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Saccharomyces cerevisiae (growth & development)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Saccharomyces cerevisiae Proteins (chemistry)</term>
<term>Saccharomyces cerevisiae Proteins (genetics)</term>
<term>Saccharomyces cerevisiae Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique (génétique)</term>
<term>ARN ribosomique (métabolisme)</term>
<term>Conformation des protéines</term>
<term>Cytoplasme (métabolisme)</term>
<term>Division cellulaire ()</term>
<term>Division cellulaire (génétique)</term>
<term>Espaceur de l'ADN ribosomique (génétique)</term>
<term>Indènes (pharmacologie)</term>
<term>Inhibiteurs de la synthèse protéique (pharmacologie)</term>
<term>Maturation post-transcriptionnelle des ARN</term>
<term>Microscopie de fluorescence</term>
<term>Modèles moléculaires</term>
<term>Mutation</term>
<term>Noyau de la cellule (métabolisme)</term>
<term>Protéines de Saccharomyces cerevisiae ()</term>
<term>Protéines de Saccharomyces cerevisiae (génétique)</term>
<term>Protéines de Saccharomyces cerevisiae (métabolisme)</term>
<term>Protéines de liaison à l'ARN (génétique)</term>
<term>Protéines de liaison à l'ARN (métabolisme)</term>
<term>Protéines ribosomiques ()</term>
<term>Protéines ribosomiques (génétique)</term>
<term>Protéines ribosomiques (métabolisme)</term>
<term>Protéines à fluorescence verte (génétique)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Précurseurs des ARN (génétique)</term>
<term>Précurseurs des ARN (métabolisme)</term>
<term>Ribosomes (génétique)</term>
<term>Ribosomes (métabolisme)</term>
<term>Saccharomyces cerevisiae (croissance et développement)</term>
<term>Saccharomyces cerevisiae (génétique)</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Technique de Western</term>
<term>Transport de protéines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ribosomal Proteins</term>
<term>Saccharomyces cerevisiae Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal Spacer</term>
<term>Green Fluorescent Proteins</term>
<term>RNA Precursors</term>
<term>RNA, Ribosomal</term>
<term>RNA-Binding Proteins</term>
<term>Ribosomal Proteins</term>
<term>Saccharomyces cerevisiae Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Division</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Division</term>
<term>Ribosomes</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique</term>
<term>Division cellulaire</term>
<term>Espaceur de l'ADN ribosomique</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de liaison à l'ARN</term>
<term>Protéines ribosomiques</term>
<term>Protéines à fluorescence verte</term>
<term>Précurseurs des ARN</term>
<term>Ribosomes</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term>
<term>Cytoplasm</term>
<term>Green Fluorescent Proteins</term>
<term>RNA Precursors</term>
<term>RNA, Ribosomal</term>
<term>RNA-Binding Proteins</term>
<term>Ribosomal Proteins</term>
<term>Ribosomes</term>
<term>Saccharomyces cerevisiae</term>
<term>Saccharomyces cerevisiae Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN ribosomique</term>
<term>Cytoplasme</term>
<term>Noyau de la cellule</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines de liaison à l'ARN</term>
<term>Protéines ribosomiques</term>
<term>Protéines à fluorescence verte</term>
<term>Précurseurs des ARN</term>
<term>Ribosomes</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Indènes</term>
<term>Inhibiteurs de la synthèse protéique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Indenes</term>
<term>Protein Synthesis Inhibitors</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term>
<term>Microscopy, Fluorescence</term>
<term>Models, Molecular</term>
<term>Mutation</term>
<term>Protein Conformation</term>
<term>Protein Transport</term>
<term>RNA Processing, Post-Transcriptional</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Conformation des protéines</term>
<term>Division cellulaire</term>
<term>Maturation post-transcriptionnelle des ARN</term>
<term>Microscopie de fluorescence</term>
<term>Modèles moléculaires</term>
<term>Mutation</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Protéines ribosomiques</term>
<term>Technique de Western</term>
<term>Transport de protéines</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Most ribosomal proteins play important roles in ribosome biogenesis and function. Here, we have examined the contribution of the essential ribosomal protein L40 in these processes in the yeast Saccharomyces cerevisiae. Deletion of either the RPL40A or RPL40B gene and in vivo depletion of L40 impair 60 S ribosomal subunit biogenesis. Polysome profile analyses reveal the accumulation of half-mers and a moderate reduction in free 60 S ribosomal subunits. Pulse-chase, Northern blotting, and primer extension analyses in the L40-depleted strain clearly indicate that L40 is not strictly required for the precursor rRNA (pre-rRNA) processing reactions but contributes to optimal 27 SB pre-rRNA maturation. Moreover, depletion of L40 hinders the nucleo-cytoplasmic export of pre-60 S ribosomal particles. Importantly, all these defects most likely appear as the direct consequence of impaired Nmd3 and Rlp24 release from cytoplasmic pre-60 S ribosomal subunits and their inefficient recycling back into the nucle(ol)us. In agreement, we show that hemagglutinin epitope-tagged L40A assembles in the cytoplasm into almost mature pre-60 S ribosomal particles. Finally, we have identified that the hemagglutinin epitope-tagged L40A confers resistance to sordarin, a translation inhibitor that impairs the function of eukaryotic elongation factor 2, whereas the rpl40a and rpl40b null mutants are hypersensitive to this antibiotic. We conclude that L40 is assembled at a very late stage into pre-60 S ribosomal subunits and that its incorporation into 60 S ribosomal subunits is a prerequisite for subunit joining and may ensure proper functioning of the translocation process.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002199 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 002199 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:22995916 |texte= Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:22995916" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |