Rapid one-step construction of a Middle East Respiratory Syndrome (MERS-CoV) infectious clone system by homologous recombination
Identifieur interne : 001047 ( Main/Curation ); précédent : 001046; suivant : 001048Rapid one-step construction of a Middle East Respiratory Syndrome (MERS-CoV) infectious clone system by homologous recombination
Auteurs : Aidan M. Nikiforuk [Canada] ; Anders Leung [Canada] ; Bradley W. M. Cook [Canada] ; Deborah A. Court [Canada] ; Darwyn Kobasa [Canada] ; Steven S. Theriault [Canada]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- genetics : Middle East Respiratory Syndrome Coronavirus.
- methods : Genetic Engineering, Reverse Genetics.
- physiology : Middle East Respiratory Syndrome Coronavirus.
- Animals, Cell Line, Homologous Recombination.
Abstract
This work describes a minimalist approach to the construction and validation of a coronavirus reverse genetics system utilizing homologous recombination for vector construction and a variety of molecular techniques for virus detection. Homologous recombination in Detection of rescued virus by molecular assay (i.e.: RT-PCR) is a strong alternative to the more traditional immunological detection assays. Rescued MERS-CoV was genomically and phenotypically similar to the original isolate MERS-CoV/EMC-2012.
Url:
DOI: 10.1016/j.jviromet.2016.07.022
PubMed: 27459876
PubMed Central: 7113859
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PMC:7113859Le document en format XML
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<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (physiology)</term>
<term>Reverse Genetics (methods)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie)</term>
<term>Génie génétique ()</term>
<term>Génétique inverse ()</term>
<term>Lignée cellulaire</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
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<front><div type="abstract" xml:lang="en"><title>Highlights</title>
<p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label>
<p id="par0005">This work describes a minimalist approach to the construction and validation of a coronavirus reverse genetics system utilizing homologous recombination for vector construction and a variety of molecular techniques for virus detection.</p>
</list-item>
<list-item id="lsti0010"><label>•</label>
<p id="par0010">Homologous recombination in <italic>S. cerevisiae</italic>
proves an efficient and fast method of constructing the large and complex genome of MERS-CoV, this strategy will be helpful for future construction of other virus genomes.</p>
</list-item>
<list-item id="lsti0015"><label>•</label>
<p id="par0015">Detection of rescued virus by molecular assay (i.e.: RT-PCR) is a strong alternative to the more traditional immunological detection assays.</p>
</list-item>
<list-item id="lsti0020"><label>•</label>
<p id="par0020">Rescued MERS-CoV was genomically and phenotypically similar to the original isolate MERS-CoV/EMC-2012.</p>
</list-item>
</list>
</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F. Almazán</name>
</author>
<author><name sortKey="Dediego, M L" uniqKey="Dediego M">M.L. Dediego</name>
</author>
<author><name sortKey="Sola, I" uniqKey="Sola I">I. Sola</name>
</author>
<author><name sortKey="Zu Iga, S" uniqKey="Zu Iga S">S. Zuñiga</name>
</author>
<author><name sortKey="Nieto Torres, J L" uniqKey="Nieto Torres J">J.L. Nieto-torres</name>
</author>
<author><name sortKey="Marquez Jurado, S" uniqKey="Marquez Jurado S">S. Marquez-jurado</name>
</author>
<author><name sortKey="Andres, G" uniqKey="Andres G">G. Andrés</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F. Almazán</name>
</author>
<author><name sortKey="Sola, I" uniqKey="Sola I">I. Sola</name>
</author>
<author><name sortKey="Zu Iga, S" uniqKey="Zu Iga S">S. Zuñiga</name>
</author>
<author><name sortKey="Marquez Jurado, S" uniqKey="Marquez Jurado S">S. Marquez-Jurado</name>
</author>
<author><name sortKey="Morales, L" uniqKey="Morales L">L. Morales</name>
</author>
<author><name sortKey="Becares, M" uniqKey="Becares M">M. Becares</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cook, B W M" uniqKey="Cook B">B.W.M. Cook</name>
</author>
<author><name sortKey="Cutts, T A" uniqKey="Cutts T">T.A. Cutts</name>
</author>
<author><name sortKey="Court, D A" uniqKey="Court D">D.A. Court</name>
</author>
<author><name sortKey="Theriault, S" uniqKey="Theriault S">S. Theriault</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ebihara, H" uniqKey="Ebihara H">H. Ebihara</name>
</author>
<author><name sortKey="Groseth, A" uniqKey="Groseth A">A. Groseth</name>
</author>
<author><name sortKey="Neumann, G" uniqKey="Neumann G">G. Neumann</name>
</author>
<author><name sortKey="Kawaoka, Y" uniqKey="Kawaoka Y">Y. Kawaoka</name>
</author>
<author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H. Feldmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ecdc" uniqKey="Ecdc">ECDC</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Eckert, K A" uniqKey="Eckert K">K.A. Eckert</name>
</author>
<author><name sortKey="Kunkel, T A" uniqKey="Kunkel T">T.A. Kunkel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gibson, D G" uniqKey="Gibson D">D.G. Gibson</name>
</author>
<author><name sortKey="Glass, J I" uniqKey="Glass J">J.I. Glass</name>
</author>
<author><name sortKey="Lartigue, C" uniqKey="Lartigue C">C. Lartigue</name>
</author>
<author><name sortKey="Noskov, V N" uniqKey="Noskov V">V.N. Noskov</name>
</author>
<author><name sortKey="Chuang, R" uniqKey="Chuang R">R. Chuang</name>
</author>
<author><name sortKey="Algire, M A" uniqKey="Algire M">M.A. Algire</name>
</author>
<author><name sortKey="Benders, G A" uniqKey="Benders G">G.A. Benders</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez, J M" uniqKey="Gonzalez J">J.M. González</name>
</author>
<author><name sortKey="Penzes, Z" uniqKey="Penzes Z">Z. Pénzes</name>
</author>
<author><name sortKey="Almazan, F" uniqKey="Almazan F">F. Almazán</name>
</author>
<author><name sortKey="Calvo, E" uniqKey="Calvo E">E. Calvo</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gorbalenya, A E" uniqKey="Gorbalenya A">A.E. Gorbalenya</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L. Enjuanes</name>
</author>
<author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J. Ziebuhr</name>
</author>
<author><name sortKey="Snijder, E J" uniqKey="Snijder E">E.J. Snijder</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hilgenfeld, R" uniqKey="Hilgenfeld R">R. Hilgenfeld</name>
</author>
<author><name sortKey="Peiris, M" uniqKey="Peiris M">M. Peiris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ma, H" uniqKey="Ma H">H. Ma</name>
</author>
<author><name sortKey="Kunes, S" uniqKey="Kunes S">S. Kunes</name>
</author>
<author><name sortKey="Schatz, P J" uniqKey="Schatz P">P.J. Schatz</name>
</author>
<author><name sortKey="Botstein, D" uniqKey="Botstein D">D. Botstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name>
</author>
<author><name sortKey="Cotten, M" uniqKey="Cotten M">M. Cotten</name>
</author>
<author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name>
</author>
<author><name sortKey="Watson, S J" uniqKey="Watson S">S.J. Watson</name>
</author>
<author><name sortKey="Alsahafi, A J" uniqKey="Alsahafi A">A.J. Alsahafi</name>
</author>
<author><name sortKey="Al Rabeeah, A A" uniqKey="Al Rabeeah A">A.A. Al Rabeeah</name>
</author>
<author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Promed Mail" uniqKey="Promed Mail">ProMED-mail</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Raj, V S" uniqKey="Raj V">V.S. Raj</name>
</author>
<author><name sortKey="Osterhaus, A D M E" uniqKey="Osterhaus A">A.D.M.E. Osterhaus</name>
</author>
<author><name sortKey="Fouchier, R A M" uniqKey="Fouchier R">R.A.M. Fouchier</name>
</author>
<author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Reusken, C B E M" uniqKey="Reusken C">C.B.E.M. Reusken</name>
</author>
<author><name sortKey="Haagmans, B L" uniqKey="Haagmans B">B.L. Haagmans</name>
</author>
<author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Müller</name>
</author>
<author><name sortKey="Gutierrez, C" uniqKey="Gutierrez C">C. Gutierrez</name>
</author>
<author><name sortKey="Godeke, G J" uniqKey="Godeke G">G.-J. Godeke</name>
</author>
<author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name>
</author>
<author><name sortKey="Muth, D" uniqKey="Muth D">D. Muth</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Scobey, T" uniqKey="Scobey T">T. Scobey</name>
</author>
<author><name sortKey="Yount, B L" uniqKey="Yount B">B.L. Yount</name>
</author>
<author><name sortKey="Sims, A C" uniqKey="Sims A">A.C. Sims</name>
</author>
<author><name sortKey="Donaldson, E F" uniqKey="Donaldson E">E.F. Donaldson</name>
</author>
<author><name sortKey="Agnihothram, S S" uniqKey="Agnihothram S">S.S. Agnihothram</name>
</author>
<author><name sortKey="Menachery, V D" uniqKey="Menachery V">V.D. Menachery</name>
</author>
<author><name sortKey="Graham, R L" uniqKey="Graham R">R.L. Graham</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stobart, C C" uniqKey="Stobart C">C.C. Stobart</name>
</author>
<author><name sortKey="Moore, M L" uniqKey="Moore M">M.L. Moore</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yount, B" uniqKey="Yount B">B. Yount</name>
</author>
<author><name sortKey="Curtis, K M" uniqKey="Curtis K">K.M. Curtis</name>
</author>
<author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yount, B" uniqKey="Yount B">B. Yount</name>
</author>
<author><name sortKey="Denison, M R" uniqKey="Denison M">M.R. Denison</name>
</author>
<author><name sortKey="Weiss, S R" uniqKey="Weiss S">S.R. Weiss</name>
</author>
<author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name>
</author>
<author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name>
</author>
<author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name>
</author>
<author><name sortKey="Osterhaus, A D M E" uniqKey="Osterhaus A">A.D.M.E. Osterhaus</name>
</author>
</analytic>
</biblStruct>
</listBibl>
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</back>
</TEI>
</record>
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