Urea can inhibit efficient reduction and alkylation of protein dimers in solution demonstrated by the beta subunit of alpha glucosidase II.
Identifieur interne : 000334 ( Main/Curation ); précédent : 000333; suivant : 000335Urea can inhibit efficient reduction and alkylation of protein dimers in solution demonstrated by the beta subunit of alpha glucosidase II.
Auteurs : Meena Kumari [États-Unis] ; Antje Anji [États-Unis]Source :
- Analytical biochemistry [ 1096-0309 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Urea, alpha-Glucosidases.
- chemical , metabolism : alpha-Glucosidases.
- methods : Electrophoresis, Gel, Two-Dimensional, Mass Spectrometry.
- Alkylation, Animals, Brain Chemistry, Mice, Protein Multimerization.
Abstract
Protein reduction and alkylation is routinely used for analysis of protein dimers and protein complexes in cell fractions using two dimensional gel electrophoresis and mass spectrometry. To resolve the heterogeneity of a high molecular weight protein band that is highlighted by an antibody to the beta subunit of alpha glucosidase II (GIIβ), we performed reduction and alkylation of cytosolic proteins extracted from mouse brain. The presence of urea in the reduction/alkylation buffer inhibited the chemical processes. It is thus recommended that protein reduction/alkylation be performed both in the presence and absence of urea for the separation of mono-/hetero-mers.
DOI: 10.1016/j.ab.2018.10.008
PubMed: 30312619
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000757
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000757
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000343
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :001F88
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :001F88
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001F88
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :000337
Links to Exploration step
pubmed:30312619Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Urea can inhibit efficient reduction and alkylation of protein dimers in solution demonstrated by the beta subunit of alpha glucosidase II.</title>
<author><name sortKey="Kumari, Meena" sort="Kumari, Meena" uniqKey="Kumari M" first="Meena" last="Kumari">Meena Kumari</name>
<affiliation wicri:level="1"><nlm:affiliation>Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 231Coles Hall, Manhattan, KS, 66506, USA. Electronic address: mkumari@vet.ksu.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 231Coles Hall, Manhattan, KS, 66506</wicri:regionArea>
<wicri:noRegion>66506</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Anji, Antje" sort="Anji, Antje" uniqKey="Anji A" first="Antje" last="Anji">Antje Anji</name>
<affiliation wicri:level="1"><nlm:affiliation>Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 231Coles Hall, Manhattan, KS, 66506, USA. Electronic address: aanji@vet.ksu.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 231Coles Hall, Manhattan, KS, 66506</wicri:regionArea>
<wicri:noRegion>66506</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2019">2019</date>
<idno type="RBID">pubmed:30312619</idno>
<idno type="pmid">30312619</idno>
<idno type="doi">10.1016/j.ab.2018.10.008</idno>
<idno type="wicri:Area/PubMed/Corpus">000757</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000757</idno>
<idno type="wicri:Area/PubMed/Curation">000757</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000757</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000343</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000343</idno>
<idno type="wicri:Area/Ncbi/Merge">001F88</idno>
<idno type="wicri:Area/Ncbi/Curation">001F88</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001F88</idno>
<idno type="wicri:Area/Main/Merge">000337</idno>
<idno type="wicri:Area/Main/Curation">000334</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Urea can inhibit efficient reduction and alkylation of protein dimers in solution demonstrated by the beta subunit of alpha glucosidase II.</title>
<author><name sortKey="Kumari, Meena" sort="Kumari, Meena" uniqKey="Kumari M" first="Meena" last="Kumari">Meena Kumari</name>
<affiliation wicri:level="1"><nlm:affiliation>Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 231Coles Hall, Manhattan, KS, 66506, USA. Electronic address: mkumari@vet.ksu.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 231Coles Hall, Manhattan, KS, 66506</wicri:regionArea>
<wicri:noRegion>66506</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Anji, Antje" sort="Anji, Antje" uniqKey="Anji A" first="Antje" last="Anji">Antje Anji</name>
<affiliation wicri:level="1"><nlm:affiliation>Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 231Coles Hall, Manhattan, KS, 66506, USA. Electronic address: aanji@vet.ksu.edu.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 231Coles Hall, Manhattan, KS, 66506</wicri:regionArea>
<wicri:noRegion>66506</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Analytical biochemistry</title>
<idno type="eISSN">1096-0309</idno>
<imprint><date when="2019" type="published">2019</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkylation</term>
<term>Animals</term>
<term>Brain Chemistry</term>
<term>Electrophoresis, Gel, Two-Dimensional (methods)</term>
<term>Mass Spectrometry (methods)</term>
<term>Mice</term>
<term>Protein Multimerization</term>
<term>Urea (chemistry)</term>
<term>alpha-Glucosidases (chemistry)</term>
<term>alpha-Glucosidases (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alkylation</term>
<term>Animaux</term>
<term>Biochimie de l'encéphale</term>
<term>Multimérisation de protéines</term>
<term>Souris</term>
<term>Spectrométrie de masse ()</term>
<term>Urée ()</term>
<term>alpha-Glucosidase ()</term>
<term>alpha-Glucosidase (métabolisme)</term>
<term>Électrophorèse bidimensionnelle sur gel ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Urea</term>
<term>alpha-Glucosidases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>alpha-Glucosidases</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Mass Spectrometry</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>alpha-Glucosidase</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Alkylation</term>
<term>Animals</term>
<term>Brain Chemistry</term>
<term>Mice</term>
<term>Protein Multimerization</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alkylation</term>
<term>Animaux</term>
<term>Biochimie de l'encéphale</term>
<term>Multimérisation de protéines</term>
<term>Souris</term>
<term>Spectrométrie de masse</term>
<term>Urée</term>
<term>alpha-Glucosidase</term>
<term>Électrophorèse bidimensionnelle sur gel</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Protein reduction and alkylation is routinely used for analysis of protein dimers and protein complexes in cell fractions using two dimensional gel electrophoresis and mass spectrometry. To resolve the heterogeneity of a high molecular weight protein band that is highlighted by an antibody to the beta subunit of alpha glucosidase II (GIIβ), we performed reduction and alkylation of cytosolic proteins extracted from mouse brain. The presence of urea in the reduction/alkylation buffer inhibited the chemical processes. It is thus recommended that protein reduction/alkylation be performed both in the presence and absence of urea for the separation of mono-/hetero-mers.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000334 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000334 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:30312619 |texte= Urea can inhibit efficient reduction and alkylation of protein dimers in solution demonstrated by the beta subunit of alpha glucosidase II. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:30312619" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |