RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in Petunia
Identifieur interne : 002681 ( Istex/Curation ); précédent : 002680; suivant : 002682RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in Petunia
Auteurs : M. Metzlaff [Royaume-Uni] ; M. O'Dell [Royaume-Uni] ; P. D Cluster [Royaume-Uni] ; R. B Flavell [Royaume-Uni]Source :
- Cell [ 0092-8674 ] ; 1997.
English descriptors
- Teeft :
- Aliquot, Amplification, Annealing, Boehringer mannheim, Cdna, Chalcone, Chalcone synthase, Chsa, Chsa coding region, Chsa endogene, Chsa rnas, Cleavage, Cleavage site, Coding region, Complementarity, Complementary sequences, Cosuppression, Degradation, Endogene, Endogene chsa, Endonucleolytic, Endonucleolytic cleavage, Endonucleolytic cleavages, Floral cosuppression, Flower tissue, Flower tissues, Fragment, High levels, Hybrida, Jorgensen, Leaf tissue, Mrna, Napoli, Nontransgenic, Oligonucleotide, Pairing, Petunia, Petunia hybrida, Plant cell, Posttranscriptional, Posttranscriptional chsa, Primer, Purple flower tissue, Purple flowers, Rna, Rnase, Sequencing, Synthase, Transgene, Transgene chsa, Transgenic, Transgenic lines, Transgenic plants, White flower tissue, White sectors.
Abstract
Abstract: Transgenic Petunia plants with a chsA coding sequence under the control of a 35S promoter sometimes lose endogene and transgene chalcone synthase activity and purple flower pigment through posttranscriptional chsA RNA degradation. In these plants, shorter poly(A)+ and poly(A)− chsA RNAs are found, and a 3′ end–specific RNA fragment from the endogene is more resistant to degradation. The termini of this RNA fragment are located in a region of complementarity between the chsA 3′ coding region and its 3′ untranslated region. Equivalent chsA RNA fragments remain in the white flower tissue of a nontransgenic Petunia variety. We present a model involving cycles of RNA–RNA pairing between complementary sequences followed by endonucleolytic RNA cleavages to describe how RNA degradation is likely to be promoted.
Url:
DOI: 10.1016/S0092-8674(00)81930-3
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002681
Links to Exploration step
ISTEX:6BA5CD05DB0BDF2B407D6291E69B9E41CB70D256Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in Petunia</title>
<author><name sortKey="Metzlaff, M" sort="Metzlaff, M" uniqKey="Metzlaff M" first="M" last="Metzlaff">M. Metzlaff</name>
<affiliation wicri:level="1"><mods:affiliation>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="O Dell, M" sort="O Dell, M" uniqKey="O Dell M" first="M" last="O'Dell">M. O'Dell</name>
<affiliation wicri:level="1"><mods:affiliation>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cluster, P D" sort="Cluster, P D" uniqKey="Cluster P" first="P. D" last="Cluster">P. D Cluster</name>
<affiliation wicri:level="1"><mods:affiliation>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Flavell, R B" sort="Flavell, R B" uniqKey="Flavell R" first="R. B" last="Flavell">R. B Flavell</name>
<affiliation wicri:level="1"><mods:affiliation>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:6BA5CD05DB0BDF2B407D6291E69B9E41CB70D256</idno>
<date when="1997" year="1997">1997</date>
<idno type="doi">10.1016/S0092-8674(00)81930-3</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-9K7V8X48-5/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002681</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002681</idno>
<idno type="wicri:Area/Istex/Curation">002681</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in Petunia</title>
<author><name sortKey="Metzlaff, M" sort="Metzlaff, M" uniqKey="Metzlaff M" first="M" last="Metzlaff">M. Metzlaff</name>
<affiliation wicri:level="1"><mods:affiliation>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="O Dell, M" sort="O Dell, M" uniqKey="O Dell M" first="M" last="O'Dell">M. O'Dell</name>
<affiliation wicri:level="1"><mods:affiliation>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cluster, P D" sort="Cluster, P D" uniqKey="Cluster P" first="P. D" last="Cluster">P. D Cluster</name>
<affiliation wicri:level="1"><mods:affiliation>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Flavell, R B" sort="Flavell, R B" uniqKey="Flavell R" first="R. B" last="Flavell">R. B Flavell</name>
<affiliation wicri:level="1"><mods:affiliation>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cell</title>
<title level="j" type="abbrev">CELL</title>
<idno type="ISSN">0092-8674</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1997">1997</date>
<biblScope unit="volume">88</biblScope>
<biblScope unit="issue">6</biblScope>
<biblScope unit="page" from="845">845</biblScope>
<biblScope unit="page" to="854">854</biblScope>
</imprint>
<idno type="ISSN">0092-8674</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0092-8674</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aliquot</term>
<term>Amplification</term>
<term>Annealing</term>
<term>Boehringer mannheim</term>
<term>Cdna</term>
<term>Chalcone</term>
<term>Chalcone synthase</term>
<term>Chsa</term>
<term>Chsa coding region</term>
<term>Chsa endogene</term>
<term>Chsa rnas</term>
<term>Cleavage</term>
<term>Cleavage site</term>
<term>Coding region</term>
<term>Complementarity</term>
<term>Complementary sequences</term>
<term>Cosuppression</term>
<term>Degradation</term>
<term>Endogene</term>
<term>Endogene chsa</term>
<term>Endonucleolytic</term>
<term>Endonucleolytic cleavage</term>
<term>Endonucleolytic cleavages</term>
<term>Floral cosuppression</term>
<term>Flower tissue</term>
<term>Flower tissues</term>
<term>Fragment</term>
<term>High levels</term>
<term>Hybrida</term>
<term>Jorgensen</term>
<term>Leaf tissue</term>
<term>Mrna</term>
<term>Napoli</term>
<term>Nontransgenic</term>
<term>Oligonucleotide</term>
<term>Pairing</term>
<term>Petunia</term>
<term>Petunia hybrida</term>
<term>Plant cell</term>
<term>Posttranscriptional</term>
<term>Posttranscriptional chsa</term>
<term>Primer</term>
<term>Purple flower tissue</term>
<term>Purple flowers</term>
<term>Rna</term>
<term>Rnase</term>
<term>Sequencing</term>
<term>Synthase</term>
<term>Transgene</term>
<term>Transgene chsa</term>
<term>Transgenic</term>
<term>Transgenic lines</term>
<term>Transgenic plants</term>
<term>White flower tissue</term>
<term>White sectors</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Transgenic Petunia plants with a chsA coding sequence under the control of a 35S promoter sometimes lose endogene and transgene chalcone synthase activity and purple flower pigment through posttranscriptional chsA RNA degradation. In these plants, shorter poly(A)+ and poly(A)− chsA RNAs are found, and a 3′ end–specific RNA fragment from the endogene is more resistant to degradation. The termini of this RNA fragment are located in a region of complementarity between the chsA 3′ coding region and its 3′ untranslated region. Equivalent chsA RNA fragments remain in the white flower tissue of a nontransgenic Petunia variety. We present a model involving cycles of RNA–RNA pairing between complementary sequences followed by endonucleolytic RNA cleavages to describe how RNA degradation is likely to be promoted.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002681 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 002681 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:6BA5CD05DB0BDF2B407D6291E69B9E41CB70D256 |texte= RNA-Mediated RNA Degradation and Chalcone Synthase A Silencing in Petunia }}
This area was generated with Dilib version V0.6.33. |