EST analysis of gene expression in testis of the ascidian Ciona intestinalis
Identifieur interne : 002362 ( Istex/Curation ); précédent : 002361; suivant : 002363EST analysis of gene expression in testis of the ascidian Ciona intestinalis
Auteurs : Kazuo Inaba [Japon] ; Potturi Padma [Japon] ; Yuhkoh Satouh [Japon] ; Tadasu Shin-I [Japon] ; Yuji Kohara [Japon] ; Nori Satoh [Japon] ; Yutaka Satou [Japon]Source :
- Molecular Reproduction and Development [ 1040-452X ] ; 2002-08.
English descriptors
- Teeft :
- Acrosome, Acrosome reaction, Agellar, Ascidian, Ascidian endostyle, Ascidian sperm, Ascidian testis, Binding proteins, Biol, Cdna, Chlamydomonas reinhardtii, Ciona, Ciona intestinalis, Ciona testis, Clone, Cytochrome, Database, Dehydrogenase, Drosophila, Drosophila melanogaster, Dynein, Dynein light chain, Embryo, Endostyle, Fertilization, Gallus, Gamete, Gene, Gene expression, Graduate school, Halocynthia, Halocynthia roretzi, High prevalence, Histone, Homo, Homolog, Hormonal regulation, Inaba, Intestinalis, Isoform, Kinase, Laevis, Male germ cells, Many genes, Marino, Mature sperm, Meiosis, Melanogaster, Mitochondrial, Mitochondrion, Morphological changes, Motility, Mrna, Musculus, Musculus protein, National institute, Nishikata, Norvegicus, Nuclear condensation, Other clusters, Other hand, Pathway, Polyadenylation factor, Polymerase, Precursor, Proc natl acad, Proteasome, Proteasome subunit, Protein, Protein kinase, Purpuratus, Rattus, Receptor, Reprod, Ribosomal, Ribosomal protein, Ribosomal proteins, Roretzi, Santis, Satoh, Satou, Sequence prevalence distribution, Sequence similarities, Sequence similarity, Sequence tags, Sertoli cells, Several genes, Sperm, Sperm motility, Spermatogenesis, Spermatogenic, Spermatogenic cells, Spermiogenesis, Strongylocentrotus, Subclass, Subunit, Synthase, Synthetase, Tailbud, Tailbud embryos, Testis, Tohoku university, Total clusters, Transcription factor, Transcriptional, Ubiquitin, Urchin, Urchin sperm, Vertebrate, Vertebrate lymphocytes, Xenopus.
Abstract
To explore the gene expression underlying spermatogenesis, a large‐scale analysis has been done on the cDNAs from testis of the ascidian, Ciona intestinalis. A set of 5,461 expressed sequence tags was analyzed and grouped into 2,806 independent clusters. Approximately 30% of the clusters showed significant sequence matches to the proteins reported in DDBJ/GenBank/EMBL database including a set of proteins closely related to the gene regulation during spermatogenesis, functional and morphological changes of spermatogenic cells during spermiogenesis, and physiological functions of sperm, as well as those with housekeeping functions commonly expressed in other cells. Some clones show similarities to the proteins present in vertebrate lymphocytes, suggesting a primitive immune system in ascidians. We have also found some genes that are known to participate in hormonal regulation of spermatogenesis in vertebrates. The large majority of the genes expressed in Ciona testis show no significant matches to known proteins and the further analysis of these genes may shed new light on the molecular mechanism of spermatogenesis and sperm functions. Mol. Reprod. Dev. 62:431–445, 2002. © 2002 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/mrd.10131
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wicri:level="1"><mods:affiliation>Correspondence address: Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Asamushi, Aomori 039‐3501, Japan.</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Correspondence address: Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Asamushi, Aomori 039‐3501</wicri:regionArea></affiliation></author><author><name sortKey="Padma, Potturi" sort="Padma, Potturi" uniqKey="Padma P" first="Potturi" last="Padma">Potturi Padma</name><affiliation wicri:level="1"><mods:affiliation>Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Asamushi, Aomori, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Asamushi, Aomori</wicri:regionArea></affiliation></author><author><name sortKey="Satouh, Yuhkoh" sort="Satouh, Yuhkoh" uniqKey="Satouh Y" 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Kyoto</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Molecular Reproduction and Development</title><title level="j" type="alt">MOLECULAR REPRODUCTION AND DEVELOPMENT</title><idno type="ISSN">1040-452X</idno><idno type="eISSN">1098-2795</idno><imprint><biblScope unit="vol">62</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="431">431</biblScope><biblScope unit="page" to="445">445</biblScope><biblScope unit="page-count">15</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="2002-08">2002-08</date></imprint><idno type="ISSN">1040-452X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1040-452X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acrosome</term><term>Acrosome reaction</term><term>Agellar</term><term>Ascidian</term><term>Ascidian endostyle</term><term>Ascidian sperm</term><term>Ascidian testis</term><term>Binding proteins</term><term>Biol</term><term>Cdna</term><term>Chlamydomonas reinhardtii</term><term>Ciona</term><term>Ciona intestinalis</term><term>Ciona testis</term><term>Clone</term><term>Cytochrome</term><term>Database</term><term>Dehydrogenase</term><term>Drosophila</term><term>Drosophila melanogaster</term><term>Dynein</term><term>Dynein light chain</term><term>Embryo</term><term>Endostyle</term><term>Fertilization</term><term>Gallus</term><term>Gamete</term><term>Gene</term><term>Gene expression</term><term>Graduate school</term><term>Halocynthia</term><term>Halocynthia roretzi</term><term>High prevalence</term><term>Histone</term><term>Homo</term><term>Homolog</term><term>Hormonal regulation</term><term>Inaba</term><term>Intestinalis</term><term>Isoform</term><term>Kinase</term><term>Laevis</term><term>Male germ cells</term><term>Many genes</term><term>Marino</term><term>Mature sperm</term><term>Meiosis</term><term>Melanogaster</term><term>Mitochondrial</term><term>Mitochondrion</term><term>Morphological changes</term><term>Motility</term><term>Mrna</term><term>Musculus</term><term>Musculus protein</term><term>National institute</term><term>Nishikata</term><term>Norvegicus</term><term>Nuclear condensation</term><term>Other clusters</term><term>Other hand</term><term>Pathway</term><term>Polyadenylation factor</term><term>Polymerase</term><term>Precursor</term><term>Proc natl acad</term><term>Proteasome</term><term>Proteasome subunit</term><term>Protein</term><term>Protein kinase</term><term>Purpuratus</term><term>Rattus</term><term>Receptor</term><term>Reprod</term><term>Ribosomal</term><term>Ribosomal protein</term><term>Ribosomal proteins</term><term>Roretzi</term><term>Santis</term><term>Satoh</term><term>Satou</term><term>Sequence prevalence distribution</term><term>Sequence similarities</term><term>Sequence similarity</term><term>Sequence tags</term><term>Sertoli cells</term><term>Several genes</term><term>Sperm</term><term>Sperm motility</term><term>Spermatogenesis</term><term>Spermatogenic</term><term>Spermatogenic cells</term><term>Spermiogenesis</term><term>Strongylocentrotus</term><term>Subclass</term><term>Subunit</term><term>Synthase</term><term>Synthetase</term><term>Tailbud</term><term>Tailbud embryos</term><term>Testis</term><term>Tohoku university</term><term>Total clusters</term><term>Transcription factor</term><term>Transcriptional</term><term>Ubiquitin</term><term>Urchin</term><term>Urchin sperm</term><term>Vertebrate</term><term>Vertebrate lymphocytes</term><term>Xenopus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To explore the gene expression underlying spermatogenesis, a large‐scale analysis has been done on the cDNAs from testis of the ascidian, Ciona intestinalis. A set of 5,461 expressed sequence tags was analyzed and grouped into 2,806 independent clusters. Approximately 30% of the clusters showed significant sequence matches to the proteins reported in DDBJ/GenBank/EMBL database including a set of proteins closely related to the gene regulation during spermatogenesis, functional and morphological changes of spermatogenic cells during spermiogenesis, and physiological functions of sperm, as well as those with housekeeping functions commonly expressed in other cells. Some clones show similarities to the proteins present in vertebrate lymphocytes, suggesting a primitive immune system in ascidians. We have also found some genes that are known to participate in hormonal regulation of spermatogenesis in vertebrates. The large majority of the genes expressed in Ciona testis show no significant matches to known proteins and the further analysis of these genes may shed new light on the molecular mechanism of spermatogenesis and sperm functions. Mol. Reprod. Dev. 62:431–445, 2002. © 2002 Wiley‐Liss, Inc.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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