Biochemical Studies on Poliovirus Polypeptide 2C: Evidence for ATPase Activity
Identifieur interne : 001F60 ( Istex/Curation ); précédent : 001F59; suivant : 001F61Biochemical Studies on Poliovirus Polypeptide 2C: Evidence for ATPase Activity
Auteurs : Caroline Mirzayan [États-Unis] ; Eckard Wimmer [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: Poliovirus 2C is a nonstructural polypeptide proposed to function in viral RNA replication. Poliovirus 2C is a member of a rapidly expanding family of proteins containing a consensus for nucleotide binding (NTP-B). Site-directed mutagenesis of conserved residues in the consensus A and S sites have suggested a functional role for the NTP-B motif in viral RNA replication and proliferation of poliovirus. We have expressed wildtype 2C and a 2C mutant, carrying a single amino acid exchange in the NTP-B motif A (Lys135Gln) using the baculovirus system. Both wildtype and mutant proteins are membrane associated. Following membrane solubilization, we have purified wildtype and mutant proteins to near homogeneity using conventional chromatography. We present biochemical evidence that wildtype 20 copurifies with an ATPase activity that is absent in the mutant preparation.
Url:
DOI: 10.1006/viro.1994.1110
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001F60
Links to Exploration step
ISTEX:B5B2C8BCC2D9D8BE88F1D6F58D6BB0714EC48E6DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Biochemical Studies on Poliovirus Polypeptide 2C: Evidence for ATPase Activity</title><author><name sortKey="Mirzayan, Caroline" sort="Mirzayan, Caroline" uniqKey="Mirzayan C" first="Caroline" last="Mirzayan">Caroline Mirzayan</name><affiliation wicri:level="2"><mods:affiliation>Department of Molecular Microbiology, State University of New York at Stony Brook, Stony Brook, New York 11794</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Molecular Microbiology, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author><author><name sortKey="Wimmer, Eckard" sort="Wimmer, Eckard" uniqKey="Wimmer E" first="Eckard" last="Wimmer">Eckard Wimmer</name><affiliation wicri:level="2"><mods:affiliation>Department of Molecular Microbiology, State University of New York at Stony Brook, Stony Brook, New York 11794</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Molecular Microbiology, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:B5B2C8BCC2D9D8BE88F1D6F58D6BB0714EC48E6D</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1006/viro.1994.1110</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-91D2WMN4-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001F60</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001F60</idno><idno type="wicri:Area/Istex/Curation">001F60</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Biochemical Studies on Poliovirus Polypeptide 2C: Evidence for ATPase Activity</title><author><name sortKey="Mirzayan, Caroline" sort="Mirzayan, Caroline" uniqKey="Mirzayan C" first="Caroline" last="Mirzayan">Caroline Mirzayan</name><affiliation wicri:level="2"><mods:affiliation>Department of Molecular Microbiology, State University of New York at Stony Brook, Stony Brook, New York 11794</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Molecular Microbiology, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author><author><name sortKey="Wimmer, Eckard" sort="Wimmer, Eckard" uniqKey="Wimmer E" first="Eckard" last="Wimmer">Eckard Wimmer</name><affiliation wicri:level="2"><mods:affiliation>Department of Molecular Microbiology, State University of New York at Stony Brook, Stony Brook, New York 11794</mods:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Molecular Microbiology, State University of New York at Stony Brook, Stony Brook</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">199</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="176">176</biblScope><biblScope unit="page" to="187">187</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Poliovirus 2C is a nonstructural polypeptide proposed to function in viral RNA replication. Poliovirus 2C is a member of a rapidly expanding family of proteins containing a consensus for nucleotide binding (NTP-B). Site-directed mutagenesis of conserved residues in the consensus A and S sites have suggested a functional role for the NTP-B motif in viral RNA replication and proliferation of poliovirus. We have expressed wildtype 2C and a 2C mutant, carrying a single amino acid exchange in the NTP-B motif A (Lys135Gln) using the baculovirus system. Both wildtype and mutant proteins are membrane associated. Following membrane solubilization, we have purified wildtype and mutant proteins to near homogeneity using conventional chromatography. We present biochemical evidence that wildtype 20 copurifies with an ATPase activity that is absent in the mutant preparation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F60 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001F60 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:B5B2C8BCC2D9D8BE88F1D6F58D6BB0714EC48E6D
|texte= Biochemical Studies on Poliovirus Polypeptide 2C: Evidence for ATPase Activity
}}
| This area was generated with Dilib version V0.6.33. |  |