Microarray analysis of Fusarium graminearum‐induced wheat genes: identification of organ‐specific and differentially expressed genes
Identifieur interne : 001C55 ( Istex/Curation ); précédent : 001C54; suivant : 001C56Microarray analysis of Fusarium graminearum‐induced wheat genes: identification of organ‐specific and differentially expressed genes
Auteurs : Saber Golkari [Canada] ; Jeannie Gilbert [Canada] ; Suvira Prashar [Canada] ; J. Douglas Procunier [Canada]Source :
- Plant Biotechnology Journal [ 1467-7644 ] ; 2007-01.
English descriptors
- Teeft :
- Abiotic stress conditions, Accession, Accession number, Anther, Arabidopsis, Arabidopsis genome, Arabidopsis thaliana, Ascorbate peroxidase, Axon instruments, Biological replications, Biotechnology, Blackwell publishing, Blight, Bract tissues, Cap3 assembly program, Cdna, Cell rescue, Cellular transport, Clone, Control plants, Database, Default parameters, Different tissues, Differentially, Disease resistance protein class, East lansing, Elongation factor, Est, Expression data, Expression levels, Expression patterns, Expression profiles, Expression ratio, Expression ratio signal intensity ratio, False discovery rate, Functional catalogue, Functional class, Functional classes, Fungal, Fungal attack, Fungal inoculation, Fungus, Fusarium, Fusarium culmorum, Fusarium graminearum, Fusarium graminearum accession number, Fusarium graminearum infection, Fusarium head blight, Fusarium head blight resistance, Fusarium wheat genes, Genbank, Genbank accession number, Genbank accession numbers, Gene, Gene expression, Gene expression patterns, Genome, Glume, Graminearum, Graminearum infection, Graminearum interaction, Heat shock protein, Homology, Homology searches, Hybridization, Hybridization efficiency, Hypothetical protein, Inner surfaces, Inoculation, Large number, Linear correlations, Michigan state university, Microarray, Microarray analysis, Microarray data, Microarray techniques, Microarrays, Minimum score, Molecular chaperones, Monodehydroascorbate reductase, Munich information centre, National fusarium head blight forum, Northern blot analysis, Nucleic acids, Other organs, Ovary, Oxidative burst, Oxidative stress, Palea, Pathogen, Pathway, Phenylpropanoid, Phenylpropanoid metabolism, Phenylpropanoid pathway, Phosphatidylinositol synthase, Plant biotechnology journal, Plant cell, Plant cell walls, Plant pathol, Plant physiol, Polyphenol oxidase, Principal components analysis, Protein, Protein sequences, Protein synthesis, Rachis, Redundant ests, Responsive organ, Ribosomal, Ribosomal protein, Rrna band intensities, Saber, Saber golkari, Salmon sperm, Salt stress, Sequence tags, Signal intensity ratios, Signal transduction pathways, Significance analysis, Significant increase, Specific organs, Specific tissues, Spike, Stringent conditions, Supplementary material, Tissue type, Tissue types, Total number, Transcript, Transcript level, Transcription factors, Transcriptome, Transport facilitation, Transport routes, Trends plant, Trichothecene mycotoxins, Triticum aestivum, Unclassified proteins, Unigenes, Unknown function, Unknown protein, Water bath, Water control, Wheat, Wheat spike, Wheat spikes, Wheat tissues, Wrky superfamily, Yellow spots.
Abstract
A wheat cDNA microarray consisting of 5739 expressed sequence tags (ESTs) was used to investigate the transcriptome patterns of the glume, lemma, palea, anther, ovary and rachis dissected from infected wheat spikes after inoculation with the fungus Fusarium graminearum, the causal agent of fusarium head blight (FHB) disease. Stringent conditions were employed to reduce the false discovery rate. The significance analysis of microarrays (SAM) was used to identify transcripts that showed a differential response between fungal‐challenged vs. control plants. To verify the microarray data, Northern blot analysis was carried out on randomly selected up‐regulated clones. We observed 185 (3.2%) up‐regulated and 16 (0.28%) down‐regulated ESTs in the six organs constituting the wheat spike. Many up‐regulated ESTs (46.67%) showed no homology with sequences of known functions, whereas others showed homology with genes involved in defence and stress responses, the oxidative burst of H2O2, regulatory functions, protein synthesis and the phenylpropanoid pathway. The monitoring of genes in specific organs avoided the averaging of expression values over multiple organs that occurs when using data from the whole spike. Our data allowed us to uncover new up‐regulated genes expressed in specific organs. The study revealed that each organ had a defined and distinctive transcriptome pattern in response to F. graminearum infection.
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DOI: 10.1111/j.1467-7652.2006.00213.x
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<term>Accession</term>
<term>Accession number</term>
<term>Anther</term>
<term>Arabidopsis</term>
<term>Arabidopsis genome</term>
<term>Arabidopsis thaliana</term>
<term>Ascorbate peroxidase</term>
<term>Axon instruments</term>
<term>Biological replications</term>
<term>Biotechnology</term>
<term>Blackwell publishing</term>
<term>Blight</term>
<term>Bract tissues</term>
<term>Cap3 assembly program</term>
<term>Cdna</term>
<term>Cell rescue</term>
<term>Cellular transport</term>
<term>Clone</term>
<term>Control plants</term>
<term>Database</term>
<term>Default parameters</term>
<term>Different tissues</term>
<term>Differentially</term>
<term>Disease resistance protein class</term>
<term>East lansing</term>
<term>Elongation factor</term>
<term>Est</term>
<term>Expression data</term>
<term>Expression levels</term>
<term>Expression patterns</term>
<term>Expression profiles</term>
<term>Expression ratio</term>
<term>Expression ratio signal intensity ratio</term>
<term>False discovery rate</term>
<term>Functional catalogue</term>
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<term>Functional classes</term>
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<term>Fusarium graminearum</term>
<term>Fusarium graminearum accession number</term>
<term>Fusarium graminearum infection</term>
<term>Fusarium head blight</term>
<term>Fusarium head blight resistance</term>
<term>Fusarium wheat genes</term>
<term>Genbank</term>
<term>Genbank accession number</term>
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<term>Microarray data</term>
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<term>National fusarium head blight forum</term>
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<term>Pathway</term>
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<term>Phenylpropanoid pathway</term>
<term>Phosphatidylinositol synthase</term>
<term>Plant biotechnology journal</term>
<term>Plant cell</term>
<term>Plant cell walls</term>
<term>Plant pathol</term>
<term>Plant physiol</term>
<term>Polyphenol oxidase</term>
<term>Principal components analysis</term>
<term>Protein</term>
<term>Protein sequences</term>
<term>Protein synthesis</term>
<term>Rachis</term>
<term>Redundant ests</term>
<term>Responsive organ</term>
<term>Ribosomal</term>
<term>Ribosomal protein</term>
<term>Rrna band intensities</term>
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<term>Saber golkari</term>
<term>Salmon sperm</term>
<term>Salt stress</term>
<term>Sequence tags</term>
<term>Signal intensity ratios</term>
<term>Signal transduction pathways</term>
<term>Significance analysis</term>
<term>Significant increase</term>
<term>Specific organs</term>
<term>Specific tissues</term>
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<term>Stringent conditions</term>
<term>Supplementary material</term>
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<term>Tissue types</term>
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<front><div type="abstract" xml:lang="en">A wheat cDNA microarray consisting of 5739 expressed sequence tags (ESTs) was used to investigate the transcriptome patterns of the glume, lemma, palea, anther, ovary and rachis dissected from infected wheat spikes after inoculation with the fungus Fusarium graminearum, the causal agent of fusarium head blight (FHB) disease. Stringent conditions were employed to reduce the false discovery rate. The significance analysis of microarrays (SAM) was used to identify transcripts that showed a differential response between fungal‐challenged vs. control plants. To verify the microarray data, Northern blot analysis was carried out on randomly selected up‐regulated clones. We observed 185 (3.2%) up‐regulated and 16 (0.28%) down‐regulated ESTs in the six organs constituting the wheat spike. Many up‐regulated ESTs (46.67%) showed no homology with sequences of known functions, whereas others showed homology with genes involved in defence and stress responses, the oxidative burst of H2O2, regulatory functions, protein synthesis and the phenylpropanoid pathway. The monitoring of genes in specific organs avoided the averaging of expression values over multiple organs that occurs when using data from the whole spike. Our data allowed us to uncover new up‐regulated genes expressed in specific organs. The study revealed that each organ had a defined and distinctive transcriptome pattern in response to F. graminearum infection.</div>
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