Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing
Identifieur interne : 001853 ( Istex/Curation ); précédent : 001852; suivant : 001854Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing
Auteurs : Stephen J. Small [États-Unis] ; Susan L. Haines [États-Unis] ; Richard A. Akeson [États-Unis]Source :
- Neuron [ 0896-6273 ] ; 1988.
English descriptors
- Teeft :
- Acad, Acid, Acid sequences, Adhesion, Adult brain, Adult forms, Akeson, Amino, Amino acid, Amino acid cells, Amino acid insertion, Amino acid sequence, Amino acids, Base alternative exon, Base exon, Base region, Binding affinity, Binding sites, Biol, Brackenbury, Cdna, Cdna clone, Cdna clones, Cdna sequence, Cell adhesion molecule, Cell adhesion molecules, Cell biol, Cell lines, Cellular basis, Chick embryo, Clone, Coding region, Constant domains, Coverslips, Differential expression, Diffuse autofluorescence, Ecorl site, Edelman, Embryonic, Embryonic brain, Exon, Experimental procedures, Exposure times, Extracellular, Extracellular domain, Extracellular domains, Genomic, Genomic clones, Genomic fragments, Heparin, Heparin binding domains, Hoffman, Hybridization, Hybridization signals, Hypervariable regions, Immunofluorescence, Immunofluorescence experiments, Immunoglobulin, Immunoglobulin superfamily, Individual cells, Insertion, Major differences, Major forms, Major polypeptide forms, Major polypeptide size classes, Major size classes, Membrane attachment, Molecule, Monoclonal antibody, Monoclonal antibody epitopes, Mrna, Mrna size class, Mrna size classes, Mrna species, Mrnas code, Muscle cells, Natl, Ncam, Nervous system, Neural, Neural cell adhesion molecule, Neural cell adhesion molecule ncam, Neural cells, Neuron, Northern blots, Nucleotide sequence, Nylon membranes, Oligo, Oligonucleotide, Other cells, Other species, Parallel coverslips, Partial cdnas, Plasma membrane, Pleated sheets, Polyclonal antibody, Polypeptide, Polypeptide diversity, Polysialic acid, Positive control, Preimmune sera, Proc, Rabbit antibody, Restriction sites, Rna, Rutishauser, Secondary structure, Separate exons, Sequence analysis, Sequencing strategy, Several species, Similar results, Size class, Smooth muscle, Splice site, Synthetic oligonucleotide, Unpublished data, Variable domains, Whole brain.
Abstract
Abstract: The alternative splicing of a previously undiscovered 30 base exon confers a new level of polypeptide diversity on the N-CAM family of cell-surface glycoproteins. It results in the insertion of 10 amino acids into the fourth of five extracellular immunoglobulin-like folds. Each major size class of rat brain N-CAM mRNAs consists of members that contain or lack the exon. Furthermore, this splicing event is developmentally controlled: RNAs containing the inserted exon are expressed at extremely low levels (<3%) in embryonic brain but increase postnatally to 40%–45% of all N-CAM mRNAs in adult brain. Antibodies that recognize the alternative 10 amino acid segment react with a subset of N-CAM-expressing neurons in cultures of embryonic rat cells.
Url:
DOI: 10.1016/0896-6273(88)90158-4
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ISTEX:1B388B5907451A711992CF54611E0ECA778B073FLe document en format XML
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<term>Acid sequences</term>
<term>Adhesion</term>
<term>Adult brain</term>
<term>Adult forms</term>
<term>Akeson</term>
<term>Amino</term>
<term>Amino acid</term>
<term>Amino acid cells</term>
<term>Amino acid insertion</term>
<term>Amino acid sequence</term>
<term>Amino acids</term>
<term>Base alternative exon</term>
<term>Base exon</term>
<term>Base region</term>
<term>Binding affinity</term>
<term>Binding sites</term>
<term>Biol</term>
<term>Brackenbury</term>
<term>Cdna</term>
<term>Cdna clone</term>
<term>Cdna clones</term>
<term>Cdna sequence</term>
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<term>Cell adhesion molecules</term>
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<term>Cellular basis</term>
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<term>Coding region</term>
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<term>Differential expression</term>
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<term>Ecorl site</term>
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<term>Embryonic brain</term>
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<term>Insertion</term>
<term>Major differences</term>
<term>Major forms</term>
<term>Major polypeptide forms</term>
<term>Major polypeptide size classes</term>
<term>Major size classes</term>
<term>Membrane attachment</term>
<term>Molecule</term>
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<term>Monoclonal antibody epitopes</term>
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<term>Mrna size class</term>
<term>Mrna size classes</term>
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<term>Nucleotide sequence</term>
<term>Nylon membranes</term>
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<term>Oligonucleotide</term>
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<term>Other species</term>
<term>Parallel coverslips</term>
<term>Partial cdnas</term>
<term>Plasma membrane</term>
<term>Pleated sheets</term>
<term>Polyclonal antibody</term>
<term>Polypeptide</term>
<term>Polypeptide diversity</term>
<term>Polysialic acid</term>
<term>Positive control</term>
<term>Preimmune sera</term>
<term>Proc</term>
<term>Rabbit antibody</term>
<term>Restriction sites</term>
<term>Rna</term>
<term>Rutishauser</term>
<term>Secondary structure</term>
<term>Separate exons</term>
<term>Sequence analysis</term>
<term>Sequencing strategy</term>
<term>Several species</term>
<term>Similar results</term>
<term>Size class</term>
<term>Smooth muscle</term>
<term>Splice site</term>
<term>Synthetic oligonucleotide</term>
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<front><div type="abstract" xml:lang="en">Abstract: The alternative splicing of a previously undiscovered 30 base exon confers a new level of polypeptide diversity on the N-CAM family of cell-surface glycoproteins. It results in the insertion of 10 amino acids into the fourth of five extracellular immunoglobulin-like folds. Each major size class of rat brain N-CAM mRNAs consists of members that contain or lack the exon. Furthermore, this splicing event is developmentally controlled: RNAs containing the inserted exon are expressed at extremely low levels (<3%) in embryonic brain but increase postnatally to 40%–45% of all N-CAM mRNAs in adult brain. Antibodies that recognize the alternative 10 amino acid segment react with a subset of N-CAM-expressing neurons in cultures of embryonic rat cells.</div>
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