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Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing

Identifieur interne : 001853 ( Istex/Curation ); précédent : 001852; suivant : 001854

Polypeptide variation in an N-CAM extracellular immunoglobulin-like fold is developmentally regulated through alternative splicing

Auteurs : Stephen J. Small [États-Unis] ; Susan L. Haines [États-Unis] ; Richard A. Akeson [États-Unis]

Source :

RBID : ISTEX:1B388B5907451A711992CF54611E0ECA778B073F

English descriptors

Abstract

Abstract: The alternative splicing of a previously undiscovered 30 base exon confers a new level of polypeptide diversity on the N-CAM family of cell-surface glycoproteins. It results in the insertion of 10 amino acids into the fourth of five extracellular immunoglobulin-like folds. Each major size class of rat brain N-CAM mRNAs consists of members that contain or lack the exon. Furthermore, this splicing event is developmentally controlled: RNAs containing the inserted exon are expressed at extremely low levels (<3%) in embryonic brain but increase postnatally to 40%–45% of all N-CAM mRNAs in adult brain. Antibodies that recognize the alternative 10 amino acid segment react with a subset of N-CAM-expressing neurons in cultures of embryonic rat cells.

Url:
DOI: 10.1016/0896-6273(88)90158-4

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ISTEX:1B388B5907451A711992CF54611E0ECA778B073F

Le document en format XML

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<term>Acid sequences</term>
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<term>Adult brain</term>
<term>Adult forms</term>
<term>Akeson</term>
<term>Amino</term>
<term>Amino acid</term>
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<term>Cell lines</term>
<term>Cellular basis</term>
<term>Chick embryo</term>
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<term>Diffuse autofluorescence</term>
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<term>Genomic clones</term>
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<term>Heparin binding domains</term>
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<term>Pleated sheets</term>
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<term>Polysialic acid</term>
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<term>Proc</term>
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<term>Sequencing strategy</term>
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<term>Similar results</term>
<term>Size class</term>
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<div type="abstract" xml:lang="en">Abstract: The alternative splicing of a previously undiscovered 30 base exon confers a new level of polypeptide diversity on the N-CAM family of cell-surface glycoproteins. It results in the insertion of 10 amino acids into the fourth of five extracellular immunoglobulin-like folds. Each major size class of rat brain N-CAM mRNAs consists of members that contain or lack the exon. Furthermore, this splicing event is developmentally controlled: RNAs containing the inserted exon are expressed at extremely low levels (<3%) in embryonic brain but increase postnatally to 40%–45% of all N-CAM mRNAs in adult brain. Antibodies that recognize the alternative 10 amino acid segment react with a subset of N-CAM-expressing neurons in cultures of embryonic rat cells.</div>
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