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Optico‐hydrodynamic properties of high molecular weight DNA from steady‐state flow birefringence and viscosity at extremely low velocity gradients

Identifieur interne : 001485 ( Istex/Curation ); précédent : 001484; suivant : 001486

Optico‐hydrodynamic properties of high molecular weight DNA from steady‐state flow birefringence and viscosity at extremely low velocity gradients

Auteurs : Rodney K. Harrington [États-Unis]

Source :

RBID : ISTEX:ACCCD0884C5D4EA7ECC70D7F9AA1B307CC1CAF52

English descriptors

Abstract

The flow birefringence, extinction angles, and shear‐dependent viscosity over a velocity gradient range of approximately 0.1–3 sec−1 have been obtained for T2 bacteriophage DNA at low concentration in neutral aqueous buffer. The data are found to be interpretable and self‐consistent in terms of subchain dynamical theory, including hydrodynamic, interactions and excluded volume, and the parameters characterizing these phenomena are in good agreement with the results of other hydrodynamic experiments and theoretical calculations. No necessity for modification of the subchain model in terms of limited extensibility or internal viscosity is found for high molecular weight DNA at the velocity gradients studied, although the latter cannot be ruled out on the basis of the present data. The Kuhn statistical segment length is determined from the intrinsic optical anisotropy and is estimated as 930 Å. Implications of these findings and their relation to appropriate dynamical models for DNA are discussed.

Url:
DOI: 10.1002/bip.1970.360090204

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ISTEX:ACCCD0884C5D4EA7ECC70D7F9AA1B307CC1CAF52

Le document en format XML

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<term>Academic press</term>
<term>Additional information</term>
<term>Anisotropy</term>
<term>Arid</term>
<term>Bead</term>
<term>Bead chain model</term>
<term>Birefringence</term>
<term>Birefringence measurements</term>
<term>Calf thymus</term>
<term>Chain distortion</term>
<term>Chain elements</term>
<term>Chain orientation</term>
<term>Chain rigidity</term>
<term>Chain stiffness</term>
<term>Chem</term>
<term>Coil expansion</term>
<term>Concentration conditions</term>
<term>Concentration data</term>
<term>Concentration dependence</term>
<term>Concentration extrapolations</term>
<term>Crothers</term>
<term>Deviation parameter</term>
<term>Dynamical</term>
<term>Dynamical details</term>
<term>Effective rigidity</term>
<term>Elastic dumbbell</term>
<term>Entire chain</term>
<term>Equivalent ellipsoid</term>
<term>Experimental conditions</term>
<term>Experimental data</term>
<term>Experimental error</term>
<term>Experimental uncertainty</term>
<term>Extensibility</term>
<term>Extinction</term>
<term>Extinction angle</term>
<term>Extinction angle data</term>
<term>Extinction angle slope</term>
<term>Extinction angles</term>
<term>Extrapolation</term>
<term>Flow birefringence</term>
<term>Flow birefringence data</term>
<term>Flow parameter</term>
<term>Form anisotropy</term>
<term>Form birefringence</term>
<term>Gaussian</term>
<term>Gaussian statistics</term>
<term>Good agreement</term>
<term>Gradient dependence</term>
<term>Higher concentrations</term>
<term>Higher shear rates</term>
<term>Higher velocity gradients</term>
<term>Hydrodynamic</term>
<term>Hydrodynamic data</term>
<term>Hydrodynamic interactions</term>
<term>Hydrodynamic properties</term>
<term>Important macromolecules</term>
<term>Inner field</term>
<term>Internal viscosity</term>
<term>Intrinsic</term>
<term>Intrinsic birefringence</term>
<term>Intrinsic flow birefringence</term>
<term>Intrinsic viscosity</term>
<term>Intrinsic viscosity data</term>
<term>Kinetic chain rigidity</term>
<term>Kinetic rigidity</term>
<term>Kuhn</term>
<term>Kuhn segment length</term>
<term>Large number</term>
<term>Large values</term>
<term>Least squares</term>
<term>Limited extensibility</term>
<term>Macromolecule</term>
<term>Molecular weight</term>
<term>Molecular weight dependence</term>
<term>Molecular weights</term>
<term>Monomer anisotropy</term>
<term>Normal coordinates</term>
<term>Normal mode</term>
<term>Numerical value</term>
<term>Optical theory</term>
<term>Optical treatment</term>
<term>Orientation time</term>
<term>Other hand</term>
<term>Other hydrodynamic</term>
<term>Other investigations</term>
<term>Overall chain</term>
<term>Overall form birefringence</term>
<term>Overall form effect</term>
<term>Parameter</term>
<term>Peterlin</term>
<term>Phys</term>
<term>Physical grounds</term>
<term>Polymer</term>
<term>Present data</term>
<term>Present study</term>
<term>Present work</term>
<term>Previous work</term>
<term>Refractive</term>
<term>Refractive index</term>
<term>Refractive indices</term>
<term>Rigidity</term>
<term>Rotational component</term>
<term>Sample preparations</term>
<term>Satisfactory agreement</term>
<term>Segmental</term>
<term>Segmental anisotropy</term>
<term>Segmental form</term>
<term>Segmental form anisotropy</term>
<term>Segmental form contributions</term>
<term>Segmental form effect</term>
<term>Segmental length</term>
<term>Serious limitation</term>
<term>Shape anisotropy</term>
<term>Shear limit</term>
<term>Shear rate</term>
<term>Shear rates</term>
<term>Shearing forces</term>
<term>Short range</term>
<term>Slight curvature</term>
<term>Standard error</term>
<term>Statistical error</term>
<term>Statistical scatter</term>
<term>Statistical segment</term>
<term>Statistical segment length</term>
<term>Statistical segments</term>
<term>Steady state flow birefringence</term>
<term>Stiffness</term>
<term>Subchain</term>
<term>Subchain model</term>
<term>Subchain theory</term>
<term>Subchains</term>
<term>Subchairi</term>
<term>Subchairi model</term>
<term>Theoretical curve</term>
<term>Tsvetkov</term>
<term>Various values</term>
<term>Velocity gradient</term>
<term>Velocity gradients</term>
<term>Vertical lines</term>
<term>Viscosity</term>
<term>Wormlike</term>
<term>Wormlike coil</term>
<term>Wormlike coil model</term>
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<div type="abstract" xml:lang="en">The flow birefringence, extinction angles, and shear‐dependent viscosity over a velocity gradient range of approximately 0.1–3 sec−1 have been obtained for T2 bacteriophage DNA at low concentration in neutral aqueous buffer. The data are found to be interpretable and self‐consistent in terms of subchain dynamical theory, including hydrodynamic, interactions and excluded volume, and the parameters characterizing these phenomena are in good agreement with the results of other hydrodynamic experiments and theoretical calculations. No necessity for modification of the subchain model in terms of limited extensibility or internal viscosity is found for high molecular weight DNA at the velocity gradients studied, although the latter cannot be ruled out on the basis of the present data. The Kuhn statistical segment length is determined from the intrinsic optical anisotropy and is estimated as 930 Å. Implications of these findings and their relation to appropriate dynamical models for DNA are discussed.</div>
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