The deposition of Notch1 in hepatitis B virus‐associated nephropathy and its role in hepatitis B virus X protein‐induced epithelial–mesenchymal transdifferentiation and immunity disorder in renal tubular epithelial cells
Identifieur interne : 001480 ( Istex/Curation ); précédent : 001479; suivant : 001481The deposition of Notch1 in hepatitis B virus‐associated nephropathy and its role in hepatitis B virus X protein‐induced epithelial–mesenchymal transdifferentiation and immunity disorder in renal tubular epithelial cells
Auteurs : X. Wang [République populaire de Chine] ; Y. Zhou [République populaire de Chine] ; N. Zhu [République populaire de Chine] ; L. Wang [République populaire de Chine] ; L. Gu [République populaire de Chine] ; W. Yuan [République populaire de Chine]Source :
- Journal of Viral Hepatitis [ 1352-0504 ] ; 2014-10.
Abstract
Notch1 plays an important role in the regulation of immune responses and epithelial–mesenchymal transdifferentiation (EMT). Previous studies have observed inflammatory cell infiltration and tubulointerstitial fibrosis in the renal biopsies from patients with HBV‐associated glomerulonephritis (HBV‐GN). We hypothesized that Notch1 may be involved in the progression of HBV‐GN. In this study, we evaluated the distribution of Notch1 in patients with HBV‐GN. Our results showed that Notch1 was mainly distributed in renal tubules and the interstitial area, and the expression levels of Notch1 had a positive correlation with the renal tubular pathology. In this respect, we used human proximal tubular epithelial cells (HK‐2) as target cells, which were transiently transfected with the hepatitis B virus X (HBx) gene using a eukaryotic vector. HBx expression resulted in significantly increased detection of Notch1, alpha‐smooth muscle actin (α‐SMA), major histocompatibility complex‐II (MHC‐II), CD40 and interleukin‐4 (IL‐4). At the same time, E‐cadherin and interferon‐γ (IFN‐γ) expression levels were significantly inhibited. These HBx‐induced phenotypes were exacerbated by upregulation of Notch1. Knock‐down of Notch1 by specific shRNA caused decreases of α‐SMA, MHC‐II, CD40 and IL‐4, and increases of E‐cadherin and IFN‐γ. These findings suggest that Notch1 is significantly associated with renal tubular and interstitial lesions. Notch1 can mediate HBx‐induced EMT of HK‐2 cells, promote HBx‐induced increases in immune molecule expression and exacerbation of cytokine disorders, which may contribute to the progression of HBV‐GN. Inhibitors of Notch1 signalling may be useful as new therapeutics for the treatment of HBV‐GN.
Url:
DOI: 10.1111/jvh.12244
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001480
Links to Exploration step
ISTEX:E2BAE190AB60309CE58DEB20FAB638C2FD9D23D5Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The deposition of Notch1 in hepatitis B virus‐associated nephropathy and its role in hepatitis B virus X protein‐induced epithelial–mesenchymal transdifferentiation and immunity disorder in renal tubular epithelial cells</title><author><name sortKey="Wang, X" sort="Wang, X" uniqKey="Wang X" first="X." last="Wang">X. Wang</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Y" sort="Zhou, Y" uniqKey="Zhou Y" first="Y." last="Zhou">Y. Zhou</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhu, N" sort="Zhu, N" uniqKey="Zhu N" first="N." last="Zhu">N. Zhu</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Wang, L" sort="Wang, L" uniqKey="Wang L" first="L." last="Wang">L. Wang</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Gu, L" sort="Gu, L" uniqKey="Gu L" first="L." last="Gu">L. Gu</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Yuan, W" sort="Yuan, W" uniqKey="Yuan W" first="W." last="Yuan">W. Yuan</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation><affiliation><mods:affiliation>Correspondence: Wei‐Jie Yuan, Division of Nephrology, Shanghai Jiaotong University Affiliated First People's Hospital, 100 HaiNing Road, Shanghai 200080, China.E‐mail:</mods:affiliation><wicri:noCountry code="subField">China.E‐mail:</wicri:noCountry></affiliation><affiliation><mods:affiliation>E-mail: ywj4169@163.com</mods:affiliation><wicri:noCountry code="no comma">E-mail: ywj4169@163.com</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:E2BAE190AB60309CE58DEB20FAB638C2FD9D23D5</idno><date when="2014" year="2014">2014</date><idno type="doi">10.1111/jvh.12244</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-P0NBSNPP-L/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001480</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001480</idno><idno type="wicri:Area/Istex/Curation">001480</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">The deposition of Notch1 in hepatitis B virus‐associated nephropathy and its role in hepatitis B virus X protein‐induced epithelial–mesenchymal transdifferentiation and immunity disorder in renal tubular epithelial cells</title><author><name sortKey="Wang, X" sort="Wang, X" uniqKey="Wang X" first="X." last="Wang">X. Wang</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhou, Y" sort="Zhou, Y" uniqKey="Zhou Y" first="Y." last="Zhou">Y. Zhou</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Zhu, N" sort="Zhu, N" uniqKey="Zhu N" first="N." last="Zhu">N. Zhu</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Wang, L" sort="Wang, L" uniqKey="Wang L" first="L." last="Wang">L. Wang</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Gu, L" sort="Gu, L" uniqKey="Gu L" first="L." last="Gu">L. Gu</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Yuan, W" sort="Yuan, W" uniqKey="Yuan W" first="W." last="Yuan">W. Yuan</name><affiliation wicri:level="1"><mods:affiliation>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai, China</mods:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Nephrology, Shanghai Jiaotong University Affiliated Shanghai First People's Hospital, Shanghai</wicri:regionArea></affiliation><affiliation><mods:affiliation>Correspondence: Wei‐Jie Yuan, Division of Nephrology, Shanghai Jiaotong University Affiliated First People's Hospital, 100 HaiNing Road, Shanghai 200080, China.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: ywj4169@163.com</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Viral Hepatitis</title><title level="j" type="alt">JOURNAL OF VIRAL HEPATITIS</title><idno type="ISSN">1352-0504</idno><idno type="eISSN">1365-2893</idno><imprint><biblScope unit="vol">21</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="734">734</biblScope><biblScope unit="page" to="743">743</biblScope><biblScope unit="page-count">10</biblScope><date type="published" when="2014-10">2014-10</date></imprint><idno type="ISSN">1352-0504</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1352-0504</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Notch1 plays an important role in the regulation of immune responses and epithelial–mesenchymal transdifferentiation (EMT). Previous studies have observed inflammatory cell infiltration and tubulointerstitial fibrosis in the renal biopsies from patients with HBV‐associated glomerulonephritis (HBV‐GN). We hypothesized that Notch1 may be involved in the progression of HBV‐GN. In this study, we evaluated the distribution of Notch1 in patients with HBV‐GN. Our results showed that Notch1 was mainly distributed in renal tubules and the interstitial area, and the expression levels of Notch1 had a positive correlation with the renal tubular pathology. In this respect, we used human proximal tubular epithelial cells (HK‐2) as target cells, which were transiently transfected with the hepatitis B virus X (HBx) gene using a eukaryotic vector. HBx expression resulted in significantly increased detection of Notch1, alpha‐smooth muscle actin (α‐SMA), major histocompatibility complex‐II (MHC‐II), CD40 and interleukin‐4 (IL‐4). At the same time, E‐cadherin and interferon‐γ (IFN‐γ) expression levels were significantly inhibited. These HBx‐induced phenotypes were exacerbated by upregulation of Notch1. Knock‐down of Notch1 by specific shRNA caused decreases of α‐SMA, MHC‐II, CD40 and IL‐4, and increases of E‐cadherin and IFN‐γ. These findings suggest that Notch1 is significantly associated with renal tubular and interstitial lesions. Notch1 can mediate HBx‐induced EMT of HK‐2 cells, promote HBx‐induced increases in immune molecule expression and exacerbation of cytokine disorders, which may contribute to the progression of HBV‐GN. Inhibitors of Notch1 signalling may be useful as new therapeutics for the treatment of HBV‐GN.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001480 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001480 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:E2BAE190AB60309CE58DEB20FAB638C2FD9D23D5
|texte= The deposition of Notch1 in hepatitis B virus‐associated nephropathy and its role in hepatitis B virus X protein‐induced epithelial–mesenchymal transdifferentiation and immunity disorder in renal tubular epithelial cells
}}
| This area was generated with Dilib version V0.6.33. |  |