Interferometric silicon biochips for label and label‐free DNA and protein microarrays
Identifieur interne : 001436 ( Istex/Curation ); précédent : 001435; suivant : 001437Interferometric silicon biochips for label and label‐free DNA and protein microarrays
Auteurs : Marina Cretich [Italie] ; Margo R. Monroe [États-Unis] ; Alex Reddington [États-Unis] ; Xirui Zhang [États-Unis] ; George G. Daaboul [États-Unis] ; Francesco Damin [Italie] ; Laura Sola [Italie] ; M. Selim Unlu [États-Unis] ; Marcella Chiari [Italie]Source :
- PROTEOMICS [ 1615-9853 ] ; 2012-10.
English descriptors
- Teeft :
- Allergen, Allergy, Allergy testing analysis, Alternative approach, Anal, Article reviews, Assay, Assay development process, Atom transfer, Atomic force microscopy, Axial locations, Binding interactions, Binding kinetics, Biochip, Bioelectron, Biomolecular interactions, Biosens, Biosensor applications, Biosensors, Boston university, Cafe method, Chem, Chemical surface reactions, Chemical vapor deposition, Chip exhibit, Collection optics, Combination chip, Conformational, Conformational change, Conformational changes, Consiglio nazionale delle ricerche, Constructive interference, Copolymer, Covalent, Covalent immobilization, Cretich, Detection, Detection schemes, Detection sensitivity, Different approaches, Dipole emission model, Direct detection, Dual detection, Dual detection scheme, Early diagnosis, Emission spectrum, Enhancement, Ensemble measurements, Fluorescence enhancement, Genetic diseases, Glass slides, Glass substrate, Glass substrates, Glass surface chemistries, Glass surfaces, Gmbh, Graft polymerization, Heterobifunctional crosslinkers, High ratio, High throughput, Homozygous subject, Hydroxyl groups, Ieee jstqe, Imaging, Immobilization, Integration host factor, Interference signature, Interferometric, Interferometric imaging sensor, Interferometric technique, Iris, Iris measurement, Kgaa, Kinetics monitoring, Large variety, Laser, Linear responses, Mass transport limitations, Maternal plasma, Microarray, Microarray applications, Microarray technology, Microarrays, Mirrorepoxy substrates, Monodimensional coatings, Monolayers, Multiplexed detection, Nanoparticle, National academy, Optical biosensors, Optical interferences, Optical interferometry, Optical response, Optical thickness, Optimized, Organic monolayers, Organosilanization reactions, Oxide, Oxide layer, Oxide thickness, Oxidized silicon substrates, Peanut allergen, Photoelectron spectroscopy, Phys, Polymer, Polymer brushes, Polymer layer, Polymeric, Polymeric coating, Polymeric coatings, Polymerization techniques, Probe, Probe molecules, Protein adsorption, Protein binding, Protein microarrays, Proteomics, Quality control, Radical polymerization, Reactive, Reactive group, Reactive groups, Recent work, Region displays, Riconoscimento molecolare, Right panel, Same protein array, Schematic representation, Second platform, Sensitive detection, Silanization, Silanization conditions, Silanization process, Silicon, Silicon biochips, Silicon chip, Silicon chips, Silicon oxide, Silicon oxide layer, Silicon slides, Silicon substrate, Silicon substrates, Single chip, Sio2, Sio2 layer, Sio2 layers, Solid substrates, Spectral oscillations, Spectroscopic ellipsometry, Square millimeter sensitivity, Square silicon slide, Ssfm, Ssfm platform, Steric hindrance, Substrate surface, Superior performance, Surface plasmon resonance, Thin silicon oxides, Timothy grass allergen, Traditional analysis, Uorescence, Uorescence assay, Uorescence detection, Uorescence enhancement, Uorescence intensity, Uorescence measurement, Uorescence microscopy, Uorescence signal, Uorescence signals, Uorescent, Uorescent microarrays, Uorescent microscopy, Uorophore, Uorophore height change, Uorophores, Verlag, Verlag gmbh, Weinheim, Weinheim figure, Weinheim proteomics, White light, Wide range.
Abstract
Protein and DNA microarrays hold the promise to revolutionize the field of molecular diagnostics. Traditional microarray applications employ labeled detection strategies based on the use of fluorescent and chemiluminescent secondary antibodies. However, the development of high throughput, sensitive, label‐free detection techniques is attracting attention as they do not require labeled reactants and provide quantitative information on binding kinetics. In this article, we will provide an overview of the recent author's work in label and label‐free sensing platforms employing silicon/silicon oxide (Si/SiO2) substrates for interferometric and/or fluorescence detection of microarrays. The review will focus on applications of Si/SiO2 with controlled oxide layers to (i) enhance the fluorescence intensity by optical interferences, (ii) quantify with sub‐nanometer accuracy the axial locations of fluorophore‐labeled probes tethered to the surface, and (iii) detect protein–protein interactions label free. Different methods of biofunctionalization of the sensing surface will be discussed. In particular, organosilanization reactions for monodimensional coatings and polymeric coatings will be extensively reviewed. Finally, the importance of calibration of protein microarrays through the dual use of labeled and label‐free detection schemes on the same chip will be illustrated.
Url:
DOI: 10.1002/pmic.201200202
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<term>Allergy</term>
<term>Allergy testing analysis</term>
<term>Alternative approach</term>
<term>Anal</term>
<term>Article reviews</term>
<term>Assay</term>
<term>Assay development process</term>
<term>Atom transfer</term>
<term>Atomic force microscopy</term>
<term>Axial locations</term>
<term>Binding interactions</term>
<term>Binding kinetics</term>
<term>Biochip</term>
<term>Bioelectron</term>
<term>Biomolecular interactions</term>
<term>Biosens</term>
<term>Biosensor applications</term>
<term>Biosensors</term>
<term>Boston university</term>
<term>Cafe method</term>
<term>Chem</term>
<term>Chemical surface reactions</term>
<term>Chemical vapor deposition</term>
<term>Chip exhibit</term>
<term>Collection optics</term>
<term>Combination chip</term>
<term>Conformational</term>
<term>Conformational change</term>
<term>Conformational changes</term>
<term>Consiglio nazionale delle ricerche</term>
<term>Constructive interference</term>
<term>Copolymer</term>
<term>Covalent</term>
<term>Covalent immobilization</term>
<term>Cretich</term>
<term>Detection</term>
<term>Detection schemes</term>
<term>Detection sensitivity</term>
<term>Different approaches</term>
<term>Dipole emission model</term>
<term>Direct detection</term>
<term>Dual detection</term>
<term>Dual detection scheme</term>
<term>Early diagnosis</term>
<term>Emission spectrum</term>
<term>Enhancement</term>
<term>Ensemble measurements</term>
<term>Fluorescence enhancement</term>
<term>Genetic diseases</term>
<term>Glass slides</term>
<term>Glass substrate</term>
<term>Glass substrates</term>
<term>Glass surface chemistries</term>
<term>Glass surfaces</term>
<term>Gmbh</term>
<term>Graft polymerization</term>
<term>Heterobifunctional crosslinkers</term>
<term>High ratio</term>
<term>High throughput</term>
<term>Homozygous subject</term>
<term>Hydroxyl groups</term>
<term>Ieee jstqe</term>
<term>Imaging</term>
<term>Immobilization</term>
<term>Integration host factor</term>
<term>Interference signature</term>
<term>Interferometric</term>
<term>Interferometric imaging sensor</term>
<term>Interferometric technique</term>
<term>Iris</term>
<term>Iris measurement</term>
<term>Kgaa</term>
<term>Kinetics monitoring</term>
<term>Large variety</term>
<term>Laser</term>
<term>Linear responses</term>
<term>Mass transport limitations</term>
<term>Maternal plasma</term>
<term>Microarray</term>
<term>Microarray applications</term>
<term>Microarray technology</term>
<term>Microarrays</term>
<term>Mirrorepoxy substrates</term>
<term>Monodimensional coatings</term>
<term>Monolayers</term>
<term>Multiplexed detection</term>
<term>Nanoparticle</term>
<term>National academy</term>
<term>Optical biosensors</term>
<term>Optical interferences</term>
<term>Optical interferometry</term>
<term>Optical response</term>
<term>Optical thickness</term>
<term>Optimized</term>
<term>Organic monolayers</term>
<term>Organosilanization reactions</term>
<term>Oxide</term>
<term>Oxide layer</term>
<term>Oxide thickness</term>
<term>Oxidized silicon substrates</term>
<term>Peanut allergen</term>
<term>Photoelectron spectroscopy</term>
<term>Phys</term>
<term>Polymer</term>
<term>Polymer brushes</term>
<term>Polymer layer</term>
<term>Polymeric</term>
<term>Polymeric coating</term>
<term>Polymeric coatings</term>
<term>Polymerization techniques</term>
<term>Probe</term>
<term>Probe molecules</term>
<term>Protein adsorption</term>
<term>Protein binding</term>
<term>Protein microarrays</term>
<term>Proteomics</term>
<term>Quality control</term>
<term>Radical polymerization</term>
<term>Reactive</term>
<term>Reactive group</term>
<term>Reactive groups</term>
<term>Recent work</term>
<term>Region displays</term>
<term>Riconoscimento molecolare</term>
<term>Right panel</term>
<term>Same protein array</term>
<term>Schematic representation</term>
<term>Second platform</term>
<term>Sensitive detection</term>
<term>Silanization</term>
<term>Silanization conditions</term>
<term>Silanization process</term>
<term>Silicon</term>
<term>Silicon biochips</term>
<term>Silicon chip</term>
<term>Silicon chips</term>
<term>Silicon oxide</term>
<term>Silicon oxide layer</term>
<term>Silicon slides</term>
<term>Silicon substrate</term>
<term>Silicon substrates</term>
<term>Single chip</term>
<term>Sio2</term>
<term>Sio2 layer</term>
<term>Sio2 layers</term>
<term>Solid substrates</term>
<term>Spectral oscillations</term>
<term>Spectroscopic ellipsometry</term>
<term>Square millimeter sensitivity</term>
<term>Square silicon slide</term>
<term>Ssfm</term>
<term>Ssfm platform</term>
<term>Steric hindrance</term>
<term>Substrate surface</term>
<term>Superior performance</term>
<term>Surface plasmon resonance</term>
<term>Thin silicon oxides</term>
<term>Timothy grass allergen</term>
<term>Traditional analysis</term>
<term>Uorescence</term>
<term>Uorescence assay</term>
<term>Uorescence detection</term>
<term>Uorescence enhancement</term>
<term>Uorescence intensity</term>
<term>Uorescence measurement</term>
<term>Uorescence microscopy</term>
<term>Uorescence signal</term>
<term>Uorescence signals</term>
<term>Uorescent</term>
<term>Uorescent microarrays</term>
<term>Uorescent microscopy</term>
<term>Uorophore</term>
<term>Uorophore height change</term>
<term>Uorophores</term>
<term>Verlag</term>
<term>Verlag gmbh</term>
<term>Weinheim</term>
<term>Weinheim figure</term>
<term>Weinheim proteomics</term>
<term>White light</term>
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<front><div type="abstract">Protein and DNA microarrays hold the promise to revolutionize the field of molecular diagnostics. Traditional microarray applications employ labeled detection strategies based on the use of fluorescent and chemiluminescent secondary antibodies. However, the development of high throughput, sensitive, label‐free detection techniques is attracting attention as they do not require labeled reactants and provide quantitative information on binding kinetics. In this article, we will provide an overview of the recent author's work in label and label‐free sensing platforms employing silicon/silicon oxide (Si/SiO2) substrates for interferometric and/or fluorescence detection of microarrays. The review will focus on applications of Si/SiO2 with controlled oxide layers to (i) enhance the fluorescence intensity by optical interferences, (ii) quantify with sub‐nanometer accuracy the axial locations of fluorophore‐labeled probes tethered to the surface, and (iii) detect protein–protein interactions label free. Different methods of biofunctionalization of the sensing surface will be discussed. In particular, organosilanization reactions for monodimensional coatings and polymeric coatings will be extensively reviewed. Finally, the importance of calibration of protein microarrays through the dual use of labeled and label‐free detection schemes on the same chip will be illustrated.</div>
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