Serum CA 125 expression as a tumor marker for diagnosis and monitoring the clinical course of epithelioid sarcoma
Identifieur interne : 001132 ( Istex/Curation ); précédent : 001131; suivant : 001133Serum CA 125 expression as a tumor marker for diagnosis and monitoring the clinical course of epithelioid sarcoma
Auteurs : Makiko Hoshino [Japon] ; Hiroyuki Kawashima [Japon] ; Akira Ogose [Japon] ; Naoko Kudo [Japon] ; Takashi Ariizumi [Japon] ; Tetsuo Hotta [Japon] ; Hajime Umezu [Japon] ; Hiroshi Hatano [Japon] ; Tetsuro Morita [Japon] ; Jyun Nishio [Japon] ; Hiroshi Iwasaki [Japon] ; Naoto Endo [Japon]Source :
- Journal of Cancer Research and Clinical Oncology [ 0171-5216 ] ; 2010-03-01.
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Abstract
Abstract: Purpose: We report here, our experience of seven patients with epithelioid sarcomas and their serum CA 125 levels, as well as the results of an in vitro and in vivo study of CA 125 expression in epithelioid sarcoma cells and xenografts using three epithelioid sarcoma cell lines. Methods: In the clinical study, the serum CA 125 levels of seven epithelioid sarcoma patients were examined at multiple time points. Expression of the MUC16 gene that encodes the CA 125 sequence was examined using RT-PCR methods in three epithelioid sarcoma cell lines, FU-EPS-1, SFT-8606 and NEPS, and the CA 125 protein in each cell lysate was examined by Western blot using anti-CA 125 clone OC125 antibody. The concentration of CA 125 in the conditioned medium of each cell line was also measured. Results: In five of the seven epithelioid sarcoma patients, CA 125 levels reflected regression and progression of their disease. The CA 125 concentrations in the conditioned medium of FU-EPS-1, SFT-8606 and NEPS cells were 259, 252, and 6 U/ml, respectively. Strong expression of MUC16 mRNA was shown in FU-EPS-1 and SFT-8606 cells: correspondingly, a thick band was observed by Western blot analysis in only FU-EPS-1 and SFT-8606 cells. Conclusion: We concluded that epithelioid sarcoma cells produce and secrete CA 125 into the blood serum and that the elevation of serum CA 125 correlates with disease progression. Therefore, measuring the serum CA 125 level should provide an useful index for diagnosing and monitoring the course of epithelioid sarcoma.
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DOI: 10.1007/s00432-009-0678-1
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Tetsuro" sort="Morita, Tetsuro" uniqKey="Morita T" first="Tetsuro" last="Morita">Tetsuro Morita</name><affiliation wicri:level="1"><mods:affiliation>Department of Orthopedic Surgery, Niigata Cancer Center Hospital, 2-15-3 Kawagishi-cho, Chuo-ku, 951-8566, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Orthopedic Surgery, Niigata Cancer Center Hospital, 2-15-3 Kawagishi-cho, Chuo-ku, 951-8566, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Nishio, Jyun" sort="Nishio, Jyun" uniqKey="Nishio J" first="Jyun" last="Nishio">Jyun Nishio</name><affiliation wicri:level="1"><mods:affiliation>Department of Orthopedic Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-0180, Fukuoka, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Orthopedic Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-0180, Fukuoka</wicri:regionArea></affiliation></author><author><name sortKey="Iwasaki, Hiroshi" sort="Iwasaki, Hiroshi" uniqKey="Iwasaki H" first="Hiroshi" last="Iwasaki">Hiroshi Iwasaki</name><affiliation wicri:level="1"><mods:affiliation>Department of Pathology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-0180, Fukuoka, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-0180, Fukuoka</wicri:regionArea></affiliation></author><author><name sortKey="Endo, Naoto" sort="Endo, Naoto" uniqKey="Endo N" first="Naoto" last="Endo">Naoto Endo</name><affiliation wicri:level="1"><mods:affiliation>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Orthopedic Surgery, 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last="Hoshino">Makiko Hoshino</name><affiliation wicri:level="1"><mods:affiliation>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Kawashima, Hiroyuki" sort="Kawashima, Hiroyuki" uniqKey="Kawashima H" first="Hiroyuki" last="Kawashima">Hiroyuki Kawashima</name><affiliation wicri:level="1"><mods:affiliation>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>E-mail: inskawa@med.niigata-u.ac.jp</mods:affiliation><country wicri:rule="url">Japon</country></affiliation></author><author><name sortKey="Ogose, Akira" sort="Ogose, Akira" uniqKey="Ogose A" first="Akira" last="Ogose">Akira Ogose</name><affiliation wicri:level="1"><mods:affiliation>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Kudo, Naoko" sort="Kudo, Naoko" uniqKey="Kudo N" first="Naoko" last="Kudo">Naoko Kudo</name><affiliation wicri:level="1"><mods:affiliation>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Ariizumi, Takashi" sort="Ariizumi, Takashi" uniqKey="Ariizumi T" first="Takashi" last="Ariizumi">Takashi Ariizumi</name><affiliation wicri:level="1"><mods:affiliation>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Hotta, Tetsuo" sort="Hotta, Tetsuo" uniqKey="Hotta T" first="Tetsuo" last="Hotta">Tetsuo Hotta</name><affiliation wicri:level="1"><mods:affiliation>Department of Surgery, Niigata University Hospital, 1 Asahimachi-dori, Chuo-ku, 951-8520, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Surgery, Niigata University Hospital, 1 Asahimachi-dori, Chuo-ku, 951-8520, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Umezu, Hajime" sort="Umezu, Hajime" uniqKey="Umezu H" first="Hajime" last="Umezu">Hajime Umezu</name><affiliation wicri:level="1"><mods:affiliation>Department of Pathology, Niigata University Hospital, 1 Asahimachi-dori, Chuo-ku, 951-8520, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, Niigata University Hospital, 1 Asahimachi-dori, Chuo-ku, 951-8520, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Hatano, Hiroshi" sort="Hatano, Hiroshi" uniqKey="Hatano H" first="Hiroshi" last="Hatano">Hiroshi Hatano</name><affiliation wicri:level="1"><mods:affiliation>Department of Orthopedic Surgery, Niigata Cancer Center Hospital, 2-15-3 Kawagishi-cho, Chuo-ku, 951-8566, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Orthopedic Surgery, Niigata Cancer Center Hospital, 2-15-3 Kawagishi-cho, Chuo-ku, 951-8566, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Morita, Tetsuro" sort="Morita, Tetsuro" uniqKey="Morita T" first="Tetsuro" last="Morita">Tetsuro Morita</name><affiliation wicri:level="1"><mods:affiliation>Department of Orthopedic Surgery, Niigata Cancer Center Hospital, 2-15-3 Kawagishi-cho, Chuo-ku, 951-8566, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Orthopedic Surgery, Niigata Cancer Center Hospital, 2-15-3 Kawagishi-cho, Chuo-ku, 951-8566, Niigata</wicri:regionArea></affiliation></author><author><name sortKey="Nishio, Jyun" sort="Nishio, Jyun" uniqKey="Nishio J" first="Jyun" last="Nishio">Jyun Nishio</name><affiliation wicri:level="1"><mods:affiliation>Department of Orthopedic Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-0180, Fukuoka, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Orthopedic Surgery, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-0180, Fukuoka</wicri:regionArea></affiliation></author><author><name sortKey="Iwasaki, Hiroshi" sort="Iwasaki, Hiroshi" uniqKey="Iwasaki H" first="Hiroshi" last="Iwasaki">Hiroshi Iwasaki</name><affiliation wicri:level="1"><mods:affiliation>Department of Pathology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-0180, Fukuoka, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, 814-0180, Fukuoka</wicri:regionArea></affiliation></author><author><name sortKey="Endo, Naoto" sort="Endo, Naoto" uniqKey="Endo N" first="Naoto" last="Endo">Naoto Endo</name><affiliation wicri:level="1"><mods:affiliation>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata, Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 1 Asahimachi-dori, Chuo-ku, 951-8510, Niigata</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Cancer Research and Clinical Oncology</title><title level="j" type="abbrev">J Cancer Res Clin Oncol</title><idno type="ISSN">0171-5216</idno><idno type="eISSN">1432-1335</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="2010-03-01">2010-03-01</date><biblScope unit="volume">136</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="457">457</biblScope><biblScope unit="page" to="464">464</biblScope></imprint><idno type="ISSN">0171-5216</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0171-5216</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>CA 125</term><term>Epithelioid sarcoma</term><term>Production</term><term>Secretion</term><term>Tumor marker</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Production</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Purpose: We report here, our experience of seven patients with epithelioid sarcomas and their serum CA 125 levels, as well as the results of an in vitro and in vivo study of CA 125 expression in epithelioid sarcoma cells and xenografts using three epithelioid sarcoma cell lines. Methods: In the clinical study, the serum CA 125 levels of seven epithelioid sarcoma patients were examined at multiple time points. Expression of the MUC16 gene that encodes the CA 125 sequence was examined using RT-PCR methods in three epithelioid sarcoma cell lines, FU-EPS-1, SFT-8606 and NEPS, and the CA 125 protein in each cell lysate was examined by Western blot using anti-CA 125 clone OC125 antibody. The concentration of CA 125 in the conditioned medium of each cell line was also measured. Results: In five of the seven epithelioid sarcoma patients, CA 125 levels reflected regression and progression of their disease. The CA 125 concentrations in the conditioned medium of FU-EPS-1, SFT-8606 and NEPS cells were 259, 252, and 6 U/ml, respectively. Strong expression of MUC16 mRNA was shown in FU-EPS-1 and SFT-8606 cells: correspondingly, a thick band was observed by Western blot analysis in only FU-EPS-1 and SFT-8606 cells. Conclusion: We concluded that epithelioid sarcoma cells produce and secrete CA 125 into the blood serum and that the elevation of serum CA 125 correlates with disease progression. Therefore, measuring the serum CA 125 level should provide an useful index for diagnosing and monitoring the course of epithelioid sarcoma.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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