Three epitopes located on the coat protein amino terminus of viruses in the bean common mosaic potyvirus subgroup
Identifieur interne : 001131 ( Istex/Curation ); précédent : 001130; suivant : 001132Three epitopes located on the coat protein amino terminus of viruses in the bean common mosaic potyvirus subgroup
Auteurs : G. I. Mink [États-Unis] ; H. J. Vetten [Allemagne] ; S. D. Wyatt [États-Unis] ; P. H. Berger [États-Unis] ; M. J. Silbernagel [États-Unis]Source :
- Archives of Virology [ 0304-8608 ] ; 1999-06-01.
Abstract
Summary: Twenty-seven of 29 strains of viruses in the bean common mosaic virus (BCMV) subgroup of legume-infecting potyviruses reacted strongly with one or more of the monoclonal antibodies (MAbs) which are known to be specific for epitopes located along the 50 amino acids which constitute the N-terminal end of the viral coat protein. Approximately one half of the virus strains reacted with the N-terminal epitope specific (NTES) MAb 4G12 which is specific for epitope E/B4, while the other half reacted with NTES MAbs 4 Aff1 or 4F9 which are specific for epitope E/B3. All but two strains contained at least one of these epitopes while no strain contained both. Competitive assays using five sequential, non-overlapping, synthetic, 10mer peptides indicated that the amino acids critical for epitope E/B3 reaction were located at positions 5, 7, and 10 from the N-terminal end of the coat protein. By deduction we postulate that the amino acids critical for epitope E/B4 are located at positions 10, 16, and 17. Because epitope E/B3 requires isoleucine at position 10 for expression whereas epitope E/B4 requires valine to be expressed, no one strain can express both epitopes. Two viruses in our tests (azuki mosaic and Dendrobium mosaic viruses) had deletions in this portion of their sequence explaining their failure to react MAbs specific for either epitope. The critical amino acids for a third epitope, E/B3A, were located at positions 16 and 17. We found no correlation between any of the three N-terminal epitopes defined in this study and the presence or absence of any biological property that we could accurately measure: i.e., symptomatology, host range, or pathotype. However, when coat protein sequences were aligned according to epitope type E/B3 or E/B4, we found that sequences within groups had high levels of identity while between group identities were low. We also found that sequences in the 3′-end non-coding region exhibited similar relationships within and between epitope groups. Two strains of BCMV (NL-4 and RU-1) were found to possess coat protein sequences typical of epitope E/B4 but 3′-NCR sequences typical of epitope E/B3. These data suggest that both strains may be the result of natural recombinants between the two epitope groups.
Url:
DOI: 10.1007/s007050050577
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001131
Links to Exploration step
ISTEX:CD4BF80D344688348D4256D265FCA230F601C04ELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Three epitopes located on the coat protein amino terminus of viruses in the bean common mosaic potyvirus subgroup</title>
<author><name sortKey="Mink, G I" sort="Mink, G I" uniqKey="Mink G" first="G. I." last="Mink">G. I. Mink</name>
<affiliation wicri:level="1"><mods:affiliation>Washington State University, WSU-Prosser, Washington, U.S.A., USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Washington State University, WSU-Prosser, Washington, U.S.A.</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Vetten, H J" sort="Vetten, H J" uniqKey="Vetten H" first="H. J." last="Vetten">H. J. Vetten</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Biochemistry and Plant Virology, Braunschweig, Germany, Germany</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Plant Virology, Braunschweig, Germany</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wyatt, S D" sort="Wyatt, S D" uniqKey="Wyatt S" first="S. D." last="Wyatt">S. D. Wyatt</name>
<affiliation wicri:level="1"><mods:affiliation>Washington State University, Department of Plant Pathology, Pullman, Washington, U.S.A., USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Washington State University, Department of Plant Pathology, Pullman, Washington, U.S.A.</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Berger, P H" sort="Berger, P H" uniqKey="Berger P" first="P. H." last="Berger">P. H. Berger</name>
<affiliation wicri:level="1"><mods:affiliation>University of Idaho, Department of Plant, Soil and Entomological Sciences, Moscow, Idaho, U.S.A., USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>University of Idaho, Department of Plant, Soil and Entomological Sciences, Moscow, Idaho, U.S.A.</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Silbernagel, M J" sort="Silbernagel, M J" uniqKey="Silbernagel M" first="M. J." last="Silbernagel">M. J. Silbernagel</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Agriculture, Agriculture Research Service, Prosser, Washington, U.S.A., USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Agriculture, Agriculture Research Service, Prosser, Washington, U.S.A.</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:CD4BF80D344688348D4256D265FCA230F601C04E</idno>
<date when="1999" year="1999">1999</date>
<idno type="doi">10.1007/s007050050577</idno>
<idno type="url">https://api.istex.fr/ark:/67375/VQC-CN4CXZD2-K/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001131</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001131</idno>
<idno type="wicri:Area/Istex/Curation">001131</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Three epitopes located on the coat protein amino terminus of viruses in the bean common mosaic potyvirus subgroup</title>
<author><name sortKey="Mink, G I" sort="Mink, G I" uniqKey="Mink G" first="G. I." last="Mink">G. I. Mink</name>
<affiliation wicri:level="1"><mods:affiliation>Washington State University, WSU-Prosser, Washington, U.S.A., USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Washington State University, WSU-Prosser, Washington, U.S.A.</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Vetten, H J" sort="Vetten, H J" uniqKey="Vetten H" first="H. J." last="Vetten">H. J. Vetten</name>
<affiliation wicri:level="1"><mods:affiliation>Institute of Biochemistry and Plant Virology, Braunschweig, Germany, Germany</mods:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institute of Biochemistry and Plant Virology, Braunschweig, Germany</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Wyatt, S D" sort="Wyatt, S D" uniqKey="Wyatt S" first="S. D." last="Wyatt">S. D. Wyatt</name>
<affiliation wicri:level="1"><mods:affiliation>Washington State University, Department of Plant Pathology, Pullman, Washington, U.S.A., USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Washington State University, Department of Plant Pathology, Pullman, Washington, U.S.A.</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Berger, P H" sort="Berger, P H" uniqKey="Berger P" first="P. H." last="Berger">P. H. Berger</name>
<affiliation wicri:level="1"><mods:affiliation>University of Idaho, Department of Plant, Soil and Entomological Sciences, Moscow, Idaho, U.S.A., USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>University of Idaho, Department of Plant, Soil and Entomological Sciences, Moscow, Idaho, U.S.A.</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Silbernagel, M J" sort="Silbernagel, M J" uniqKey="Silbernagel M" first="M. J." last="Silbernagel">M. J. Silbernagel</name>
<affiliation wicri:level="1"><mods:affiliation>Department of Agriculture, Agriculture Research Service, Prosser, Washington, U.S.A., USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Agriculture, Agriculture Research Service, Prosser, Washington, U.S.A.</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Archives of Virology</title>
<title level="j" type="abbrev">Arch. Virol.</title>
<idno type="ISSN">0304-8608</idno>
<idno type="eISSN">1432-8798</idno>
<imprint><publisher>Springer-Verlag</publisher>
<pubPlace>Vienna</pubPlace>
<date type="published" when="1999-06-01">1999-06-01</date>
<biblScope unit="volume">144</biblScope>
<biblScope unit="issue">6</biblScope>
<biblScope unit="page" from="1173">1173</biblScope>
<biblScope unit="page" to="1189">1189</biblScope>
</imprint>
<idno type="ISSN">0304-8608</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0304-8608</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Summary: Twenty-seven of 29 strains of viruses in the bean common mosaic virus (BCMV) subgroup of legume-infecting potyviruses reacted strongly with one or more of the monoclonal antibodies (MAbs) which are known to be specific for epitopes located along the 50 amino acids which constitute the N-terminal end of the viral coat protein. Approximately one half of the virus strains reacted with the N-terminal epitope specific (NTES) MAb 4G12 which is specific for epitope E/B4, while the other half reacted with NTES MAbs 4 Aff1 or 4F9 which are specific for epitope E/B3. All but two strains contained at least one of these epitopes while no strain contained both. Competitive assays using five sequential, non-overlapping, synthetic, 10mer peptides indicated that the amino acids critical for epitope E/B3 reaction were located at positions 5, 7, and 10 from the N-terminal end of the coat protein. By deduction we postulate that the amino acids critical for epitope E/B4 are located at positions 10, 16, and 17. Because epitope E/B3 requires isoleucine at position 10 for expression whereas epitope E/B4 requires valine to be expressed, no one strain can express both epitopes. Two viruses in our tests (azuki mosaic and Dendrobium mosaic viruses) had deletions in this portion of their sequence explaining their failure to react MAbs specific for either epitope. The critical amino acids for a third epitope, E/B3A, were located at positions 16 and 17. We found no correlation between any of the three N-terminal epitopes defined in this study and the presence or absence of any biological property that we could accurately measure: i.e., symptomatology, host range, or pathotype. However, when coat protein sequences were aligned according to epitope type E/B3 or E/B4, we found that sequences within groups had high levels of identity while between group identities were low. We also found that sequences in the 3′-end non-coding region exhibited similar relationships within and between epitope groups. Two strains of BCMV (NL-4 and RU-1) were found to possess coat protein sequences typical of epitope E/B4 but 3′-NCR sequences typical of epitope E/B3. These data suggest that both strains may be the result of natural recombinants between the two epitope groups.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001131 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001131 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:CD4BF80D344688348D4256D265FCA230F601C04E |texte= Three epitopes located on the coat protein amino terminus of viruses in the bean common mosaic potyvirus subgroup }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |