Determination of Local Refractive Index for Protein and Virus Crystals in Solution by Mach-Zehnder Interferometry
Identifieur interne : 001041 ( Istex/Curation ); précédent : 001040; suivant : 001042Determination of Local Refractive Index for Protein and Virus Crystals in Solution by Mach-Zehnder Interferometry
Auteurs : T. Cole [États-Unis] ; A. Kathman [États-Unis] ; S. Koszelak [États-Unis] ; A. Mcpherson [États-Unis]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 1995.
Abstract
Abstract: To establish the importance of, and quantitatively evaluate, the macromolecular concentration gradients in the neighborhood of growing protein, virus, and nucleic acid crystals, a convenient, accurate, and nonintrusive method has been devised. This approach should prove particularly relevant in the rigorous comparison of crystals grown in a conventional laboratory setting with those grown in a microgravity environment. The method is based on precise determination of the local refractive index using Mach-Zehnder interferometry. Presented here are data for five protein and three virus systems. From data for these and other systems, optical monitoring experiments to measure local growth conditions and growth kinetics in liquid-liquid diffusion, batch, and vapor diffusion crystal growth experiments can be designed.
Url:
DOI: 10.1006/abio.1995.1507
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001041
Links to Exploration step
ISTEX:88F138680F6E2CFE5F4FEB96E9F348CCDDB0D1C7Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Determination of Local Refractive Index for Protein and Virus Crystals in Solution by Mach-Zehnder Interferometry</title>
<author><name sortKey="Cole, T" sort="Cole, T" uniqKey="Cole T" first="T." last="Cole">T. Cole</name>
<affiliation wicri:level="1"><mods:affiliation>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521, USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kathman, A" sort="Kathman, A" uniqKey="Kathman A" first="A." last="Kathman">A. Kathman</name>
<affiliation wicri:level="1"><mods:affiliation>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521, USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Koszelak, S" sort="Koszelak, S" uniqKey="Koszelak S" first="S." last="Koszelak">S. Koszelak</name>
<affiliation wicri:level="1"><mods:affiliation>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521, USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mcpherson, A" sort="Mcpherson, A" uniqKey="Mcpherson A" first="A." last="Mcpherson">A. Mcpherson</name>
<affiliation wicri:level="1"><mods:affiliation>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521, USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:88F138680F6E2CFE5F4FEB96E9F348CCDDB0D1C7</idno>
<date when="1995" year="1995">1995</date>
<idno type="doi">10.1006/abio.1995.1507</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-3R2S0TST-X/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001041</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001041</idno>
<idno type="wicri:Area/Istex/Curation">001041</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Determination of Local Refractive Index for Protein and Virus Crystals in Solution by Mach-Zehnder Interferometry</title>
<author><name sortKey="Cole, T" sort="Cole, T" uniqKey="Cole T" first="T." last="Cole">T. Cole</name>
<affiliation wicri:level="1"><mods:affiliation>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521, USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kathman, A" sort="Kathman, A" uniqKey="Kathman A" first="A." last="Kathman">A. Kathman</name>
<affiliation wicri:level="1"><mods:affiliation>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521, USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Koszelak, S" sort="Koszelak, S" uniqKey="Koszelak S" first="S." last="Koszelak">S. Koszelak</name>
<affiliation wicri:level="1"><mods:affiliation>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521, USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mcpherson, A" sort="Mcpherson, A" uniqKey="Mcpherson A" first="A." last="Mcpherson">A. Mcpherson</name>
<affiliation wicri:level="1"><mods:affiliation>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521, USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Teledyne Brown Engn Inc, Huntsville, AL 35807, USA and Univ Calif Riverside, Dept Biochem, Riverside, CA 92521</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Analytical Biochemistry</title>
<title level="j" type="abbrev">YABIO</title>
<idno type="ISSN">0003-2697</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1995">1995</date>
<biblScope unit="volume">231</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="92">92</biblScope>
<biblScope unit="page" to="98">98</biblScope>
</imprint>
<idno type="ISSN">0003-2697</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0003-2697</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: To establish the importance of, and quantitatively evaluate, the macromolecular concentration gradients in the neighborhood of growing protein, virus, and nucleic acid crystals, a convenient, accurate, and nonintrusive method has been devised. This approach should prove particularly relevant in the rigorous comparison of crystals grown in a conventional laboratory setting with those grown in a microgravity environment. The method is based on precise determination of the local refractive index using Mach-Zehnder interferometry. Presented here are data for five protein and three virus systems. From data for these and other systems, optical monitoring experiments to measure local growth conditions and growth kinetics in liquid-liquid diffusion, batch, and vapor diffusion crystal growth experiments can be designed.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001041 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001041 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:88F138680F6E2CFE5F4FEB96E9F348CCDDB0D1C7 |texte= Determination of Local Refractive Index for Protein and Virus Crystals in Solution by Mach-Zehnder Interferometry }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |