Replication timing of the human X-inactivation center (XIC) region: correlation with chromosome bands
Identifieur interne : 000E83 ( Istex/Curation ); précédent : 000E82; suivant : 000E84Replication timing of the human X-inactivation center (XIC) region: correlation with chromosome bands
Auteurs : Yoshihisa Watanabe [Japon] ; Toyoaki Tenzen [Japon] ; Yasuhiko Nagasaka [Japon] ; Hidetoshi Inoko [Japon] ; Toshimichi Ikemura [Japon]Source :
- Gene [ 0378-1119 ] ; 2000.
English descriptors
- Teeft :
- Band boundaries, Band boundary, Bernardi, Brdu, Chromosome, Chromosome bands, Class junction, Constant amount, Fukagawa, Genbank accession, Genome, Genome sequence level, Human chromosome, Human genome, Human genomic, Ikemura, Locus, Phka1, Plasmid, Plasmid pkf3, Primer, Primer sets, Repetitive, Repetitive sequences, Replication, Replication timing, Room temperature, Tenzen, Uorescence scanning system, Watanabe, Xist, Xist locus, Xist region.
Abstract
Abstract: The human genome is composed of long-range G+C% mosaic structures, which are thought to be related to chromosome bands. Replication timing during S phase is associated with chromosomal band zones; thus, band boundaries are thought to correspond to regions where replication timing switches. The proximal limit of the human X-inactivation center (XIC) has been localized cytologically to the junction zone between Xq13.1 and Xq13.2. Using PCR-based quantification of the newly replicated DNA from cell-cycle fractionated THP-1 cells, the replication timing in and around the XIC was determined at the genome sequence level. We found two regions where replication timing changes from the early to late period during S phase. One is located near a large inverted duplication proximal to the XIC, and the other is near the XIST locus. We propose that the 1Mb late-replicated zone (from the large inverted duplication to XIST) corresponds to a G-band Xq13.2. Several common characteristics were observed in the XIST region and the MHC class II–III junction which was previously defined as a band boundary. These characteristics included differential high-density clustering of Alu and LINE repeats, and the presence of polypurine/polypyrimidine tracts, MER41A, MER57 and MER58B.
Url:
DOI: 10.1016/S0378-1119(00)00208-0
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