Oligonucleotide inhibition of the interaction of HIV-1 Tat protein with the trans -activation responsive region (TAR) of HIV RNA
Identifieur interne : 000979 ( Istex/Curation ); précédent : 000978; suivant : 000980Oligonucleotide inhibition of the interaction of HIV-1 Tat protein with the trans -activation responsive region (TAR) of HIV RNA
Auteurs : Béatrice Mestre [Royaume-Uni] ; Andrey Arzumanov [Royaume-Uni] ; Mohinder Singh [Royaume-Uni] ; Florence Boulmé [France] ; Simon Litvak [France] ; Michael J. Gait [Royaume-Uni]Source :
- BBA - Gene Structure and Expression [ 0167-4781 ] ; 1999.
English descriptors
- Teeft :
- Acta, Analogue, Antisense, Antisense oligonucleotides, Apical, Apical loop, Assay, Bbaexp, Biochimica, Biophysica, Biophysica acta, Bulge region, Chem, Control oligonucleotides, Hoechst, Human virus, Human virus type, Inhibition, Inhibitor, Karn, Lter, Lter binding, Lter binding assay, Lter binding interference assays, Lter retention, Mestre, Mobility shift analysis, Mobility shift assay, Native polyacrylamide, Neomycin, Nucleic acids, Oligodeoxyribonucleotides, Oligonucleotide, Oligonucleotide inhibition, Oligonucleotides, Oligoribonucleotide, Oligoribonucleotides, Oxford university press, Peptide, Peptide binding, Practical approach, Pyrimidine, Recognition sequence, Responsive region, Retarded band, Room temperature, Synthetic oligonucleotides, Transcription, Tris acetate.
Abstract
Abstract: The interaction of HIV-1 Tat protein with its recognition sequence, the trans-activation responsive region TAR is a potential target for drug discovery against HIV infection. We show by use of an in vitro competition filter binding interference assay that synthetic oligodeoxyribonucleotides complementary to the HIV-1 TAR RNA apical stem-loop and bulge region inhibit the binding of Tat protein or a Tat peptide (residues 37–72) better than two small molecules that have been shown to bind TAR RNA, Hoechst 33258 and neomycin B. The inhibition is not sensitive to length between 13 and 16 residues or precise positioning but shorter oligonucleotides are less effective. Enhanced inhibition was obtained for a 16-mer 2′-O-methyl oligoribonucleotide but not for C5-propyne pyrimidine-substituted oligonucleotides. Control non-antisense oligonucleotides were occasionally also effective in filter binding interference but only the complementary antisense 2′-O-methyl oligoribonucleotide was effective in gel mobility shift assays in direct TAR binding or in interference with Tat peptide binding to the TAR stem-loop. This is the first demonstration of effective inhibition of the Tat-TAR interaction by nuclease-stabilized oligonucleotide analogues.
Url:
DOI: 10.1016/S0167-4781(99)00019-6
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000979
Links to Exploration step
ISTEX:282A80C882C9652F58B797865503362587A61995Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Oligonucleotide inhibition of the interaction of HIV-1 Tat protein with the trans -activation responsive region (TAR) of HIV RNA</title>
<author><name sortKey="Mestre, Beatrice" sort="Mestre, Beatrice" uniqKey="Mestre B" first="Béatrice" last="Mestre">Béatrice Mestre</name>
<affiliation wicri:level="1"><mods:affiliation>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Arzumanov, Andrey" sort="Arzumanov, Andrey" uniqKey="Arzumanov A" first="Andrey" last="Arzumanov">Andrey Arzumanov</name>
<affiliation wicri:level="1"><mods:affiliation>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Singh, Mohinder" sort="Singh, Mohinder" uniqKey="Singh M" first="Mohinder" last="Singh">Mohinder Singh</name>
<affiliation wicri:level="1"><mods:affiliation>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Boulme, Florence" sort="Boulme, Florence" uniqKey="Boulme F" first="Florence" last="Boulmé">Florence Boulmé</name>
<affiliation wicri:level="1"><mods:affiliation>EP-630, CNRS-Université Victor Ségalen Bordeaux 2, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>EP-630, CNRS-Université Victor Ségalen Bordeaux 2, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Litvak, Simon" sort="Litvak, Simon" uniqKey="Litvak S" first="Simon" last="Litvak">Simon Litvak</name>
<affiliation wicri:level="1"><mods:affiliation>EP-630, CNRS-Université Victor Ségalen Bordeaux 2, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>EP-630, CNRS-Université Victor Ségalen Bordeaux 2, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gait, Michael J" sort="Gait, Michael J" uniqKey="Gait M" first="Michael J" last="Gait">Michael J. Gait</name>
<affiliation wicri:level="1"><mods:affiliation>E-mail: mgait@mrc-lmb.cam.ac.uk</mods:affiliation>
<country wicri:rule="url">Royaume-Uni</country>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:282A80C882C9652F58B797865503362587A61995</idno>
<date when="1999" year="1999">1999</date>
<idno type="doi">10.1016/S0167-4781(99)00019-6</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-7261QRW9-Z/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000979</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000979</idno>
<idno type="wicri:Area/Istex/Curation">000979</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Oligonucleotide inhibition of the interaction of HIV-1 Tat protein with the trans -activation responsive region (TAR) of HIV RNA</title>
<author><name sortKey="Mestre, Beatrice" sort="Mestre, Beatrice" uniqKey="Mestre B" first="Béatrice" last="Mestre">Béatrice Mestre</name>
<affiliation wicri:level="1"><mods:affiliation>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Arzumanov, Andrey" sort="Arzumanov, Andrey" uniqKey="Arzumanov A" first="Andrey" last="Arzumanov">Andrey Arzumanov</name>
<affiliation wicri:level="1"><mods:affiliation>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Singh, Mohinder" sort="Singh, Mohinder" uniqKey="Singh M" first="Mohinder" last="Singh">Mohinder Singh</name>
<affiliation wicri:level="1"><mods:affiliation>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Boulme, Florence" sort="Boulme, Florence" uniqKey="Boulme F" first="Florence" last="Boulmé">Florence Boulmé</name>
<affiliation wicri:level="1"><mods:affiliation>EP-630, CNRS-Université Victor Ségalen Bordeaux 2, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>EP-630, CNRS-Université Victor Ségalen Bordeaux 2, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Litvak, Simon" sort="Litvak, Simon" uniqKey="Litvak S" first="Simon" last="Litvak">Simon Litvak</name>
<affiliation wicri:level="1"><mods:affiliation>EP-630, CNRS-Université Victor Ségalen Bordeaux 2, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex, France</mods:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>EP-630, CNRS-Université Victor Ségalen Bordeaux 2, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gait, Michael J" sort="Gait, Michael J" uniqKey="Gait M" first="Michael J" last="Gait">Michael J. Gait</name>
<affiliation wicri:level="1"><mods:affiliation>E-mail: mgait@mrc-lmb.cam.ac.uk</mods:affiliation>
<country wicri:rule="url">Royaume-Uni</country>
</affiliation>
<affiliation wicri:level="1"><mods:affiliation>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK</mods:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH</wicri:regionArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">BBA - Gene Structure and Expression</title>
<title level="j" type="abbrev">BBAEXP</title>
<idno type="ISSN">0167-4781</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1999">1999</date>
<biblScope unit="volume">1445</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="86">86</biblScope>
<biblScope unit="page" to="98">98</biblScope>
</imprint>
<idno type="ISSN">0167-4781</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0167-4781</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acta</term>
<term>Analogue</term>
<term>Antisense</term>
<term>Antisense oligonucleotides</term>
<term>Apical</term>
<term>Apical loop</term>
<term>Assay</term>
<term>Bbaexp</term>
<term>Biochimica</term>
<term>Biophysica</term>
<term>Biophysica acta</term>
<term>Bulge region</term>
<term>Chem</term>
<term>Control oligonucleotides</term>
<term>Hoechst</term>
<term>Human virus</term>
<term>Human virus type</term>
<term>Inhibition</term>
<term>Inhibitor</term>
<term>Karn</term>
<term>Lter</term>
<term>Lter binding</term>
<term>Lter binding assay</term>
<term>Lter binding interference assays</term>
<term>Lter retention</term>
<term>Mestre</term>
<term>Mobility shift analysis</term>
<term>Mobility shift assay</term>
<term>Native polyacrylamide</term>
<term>Neomycin</term>
<term>Nucleic acids</term>
<term>Oligodeoxyribonucleotides</term>
<term>Oligonucleotide</term>
<term>Oligonucleotide inhibition</term>
<term>Oligonucleotides</term>
<term>Oligoribonucleotide</term>
<term>Oligoribonucleotides</term>
<term>Oxford university press</term>
<term>Peptide</term>
<term>Peptide binding</term>
<term>Practical approach</term>
<term>Pyrimidine</term>
<term>Recognition sequence</term>
<term>Responsive region</term>
<term>Retarded band</term>
<term>Room temperature</term>
<term>Synthetic oligonucleotides</term>
<term>Transcription</term>
<term>Tris acetate</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The interaction of HIV-1 Tat protein with its recognition sequence, the trans-activation responsive region TAR is a potential target for drug discovery against HIV infection. We show by use of an in vitro competition filter binding interference assay that synthetic oligodeoxyribonucleotides complementary to the HIV-1 TAR RNA apical stem-loop and bulge region inhibit the binding of Tat protein or a Tat peptide (residues 37–72) better than two small molecules that have been shown to bind TAR RNA, Hoechst 33258 and neomycin B. The inhibition is not sensitive to length between 13 and 16 residues or precise positioning but shorter oligonucleotides are less effective. Enhanced inhibition was obtained for a 16-mer 2′-O-methyl oligoribonucleotide but not for C5-propyne pyrimidine-substituted oligonucleotides. Control non-antisense oligonucleotides were occasionally also effective in filter binding interference but only the complementary antisense 2′-O-methyl oligoribonucleotide was effective in gel mobility shift assays in direct TAR binding or in interference with Tat peptide binding to the TAR stem-loop. This is the first demonstration of effective inhibition of the Tat-TAR interaction by nuclease-stabilized oligonucleotide analogues.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000979 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000979 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:282A80C882C9652F58B797865503362587A61995 |texte= Oligonucleotide inhibition of the interaction of HIV-1 Tat protein with the trans -activation responsive region (TAR) of HIV RNA }}
This area was generated with Dilib version V0.6.33. |