Respiratory Syncytial Virus Infection Induces Expression of the Anti-Apoptosis Gene IEX-1L in Human Respiratory Epithelial Cells
Identifieur interne : 000875 ( Istex/Curation ); précédent : 000874; suivant : 000876Respiratory Syncytial Virus Infection Induces Expression of the Anti-Apoptosis Gene IEX-1L in Human Respiratory Epithelial Cells
Auteurs : Joseph B. Domachowske [États-Unis] ; Cynthia A. Bonville [États-Unis] ; Anthony J. Mortelliti [États-Unis] ; Carol B. Colella [États-Unis] ; Urian Kim [États-Unis] ; Helene F. Rosenberg [États-Unis]Source :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2000.
Abstract
By means of differential display reverse-transcriptase polymerase chain reaction, increased expression of the mRNA encoding the anti-apoptosis gene IEX-1L was found in respiratory epithelial cells infected with respiratory syncytial virus (RSV). IEX-1L mRNA expression increased 5–7-fold in RSV-infected cells at 72 h after infection but remained unchanged in cells exposed to irradiated, replication-incompetent RSV. Because IEX-1L is reported to protect cells from apoptosis induced by tumor necrosis factor (TNF)-α, the effect of TNF-α on epithelial cell apoptosis in the context of RSV infection was determined. Epithelial cells were exposed to vehicle, RSV, or irradiated RSV for 72 h, and then TNF-α was added to appropriate cultures. Cytochemical staining of cellular DNA with 4,6-diamidino-2-phenylindole demonstrated TNF-α-induced apoptosis in 23.4% of control cells but only 5% of RSV-infected cells. These data show that RSV infection protects epithelial cells from TNF-α-induced apoptosis and that this effect is temporally associated with IEX-1L gene expression.
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DOI: 10.1086/315319
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<front><div type="abstract">By means of differential display reverse-transcriptase polymerase chain reaction, increased expression of the mRNA encoding the anti-apoptosis gene IEX-1L was found in respiratory epithelial cells infected with respiratory syncytial virus (RSV). IEX-1L mRNA expression increased 5–7-fold in RSV-infected cells at 72 h after infection but remained unchanged in cells exposed to irradiated, replication-incompetent RSV. Because IEX-1L is reported to protect cells from apoptosis induced by tumor necrosis factor (TNF)-α, the effect of TNF-α on epithelial cell apoptosis in the context of RSV infection was determined. Epithelial cells were exposed to vehicle, RSV, or irradiated RSV for 72 h, and then TNF-α was added to appropriate cultures. Cytochemical staining of cellular DNA with 4,6-diamidino-2-phenylindole demonstrated TNF-α-induced apoptosis in 23.4% of control cells but only 5% of RSV-infected cells. These data show that RSV infection protects epithelial cells from TNF-α-induced apoptosis and that this effect is temporally associated with IEX-1L gene expression.</div>
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