Identification of a murine CD4+ T-lymphocyte response site in hepatitis C virus core protein
Identifieur interne : 000764 ( Istex/Curation ); précédent : 000763; suivant : 000765Identification of a murine CD4+ T-lymphocyte response site in hepatitis C virus core protein
Auteurs : Ziping Chen [États-Unis] ; Ira Berkower [États-Unis] ; Wei-Mei Ching [États-Unis] ; R. Yuan-Hu Wang [États-Unis] ; Harvey J. Alter [États-Unis] ; J. Wai-Kuo Shih [États-Unis]Source :
- Molecular Immunology [ 0161-5890 ] ; 1996.
English descriptors
- Teeft :
- Acad, Amino, Amino acid, Amino acids, Antibody response, Antibody titers, Antigen processing, Cell epitope, Cell helper epitope, Cell response, Cell responses, Cell site, Chen, Choo, Chronic hepatitis, Chronic infection, Core antigen, Core peptides, Core protein, Cytotoxic, Disease prevention, Elsevier science, Epitope, Epitope size, Helper, Helper effect, Helper epitope, Hepatitis, Immune, Immune response, Inclusion bodies, Lymph node cells, Lymphocyte, Major epitope, Murine humoral, Natn, Nucleotide sequence, Other peptides, Peptide, Peptides declines, Popliteal lymph node cells, Possible roles, Prime helper, Proc, Proliferation assay, Prospective study, Recombinant, Same buffer, Specific proliferation, Stimulation index, Synthetic peptide, Synthetic peptides, Vaccine, Viral, Viral infection, Virus antigens, Virus core protein, Virus genome, Virus infection, Wang, Whole protein.
Abstract
Abstract: The T cell response to a recombinant HCV truncated core protein (cp1-10) was measured in a proliferation assay. Based on a 10-fold greater response to this truncated core protein than to its shorter form (cp1-8), a predominant epitope was mapped to the carboxyl quarter of this sequence. This epitope was further mapped to a synthetic peptide corresponding to amino acids 121–140 of the core protein. The peptide was antigenic for T cells of all three H-2 types tested, H-2 r, b and d, and the proliferating T cells were CD4+. Besides inducing specific proliferation in vitro, peptide aa121–140 can prime helper T cells in vivo. When boosted with core protein, mice primed with peptide produced 64-fold higher antibody titer than without priming in 1 week. The identification of a broadly immunogenic T cell helper epitope on core protein may be important for vaccine design against HCV.
Url:
DOI: 10.1016/0161-5890(96)00010-7
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Based on a 10-fold greater response to this truncated core protein than to its shorter form (cp1-8), a predominant epitope was mapped to the carboxyl quarter of this sequence. This epitope was further mapped to a synthetic peptide corresponding to amino acids 121–140 of the core protein. The peptide was antigenic for T cells of all three H-2 types tested, H-2 r, b and d, and the proliferating T cells were CD4+. Besides inducing specific proliferation in vitro, peptide aa121–140 can prime helper T cells in vivo. When boosted with core protein, mice primed with peptide produced 64-fold higher antibody titer than without priming in 1 week. The identification of a broadly immunogenic T cell helper epitope on core protein may be important for vaccine design against HCV.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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