Cloning and molecular characterization of a telomeric sequence from a temperature-induced Balbiani ring
Identifieur interne : 000687 ( Istex/Curation ); précédent : 000686; suivant : 000688Cloning and molecular characterization of a telomeric sequence from a temperature-induced Balbiani ring
Auteurs : M. J. Carmona [Espagne] ; G. Morcillo [Espagne] ; R. Galler [Allemagne] ; E. Martínez-Salas [Espagne] ; A. G. De La Campa [Espagne] ; J. L. Díez [Espagne] ; J. E. Edström [Allemagne]Source :
- Chromosoma [ 0009-5915 ] ; 1985-06-01.
English descriptors
- Teeft :
- Chironomus, Chironomus thummi, Chromosoma, Chromosomal, Chromosome, Chromosome ends, Clone, Digestion, Drosophila, Enzo biochem, Genome, Genomic, Heat shock, Hybridization, Hybridized, Larva, Molecular characterization, Morcillo, Nitrocellulose, Nitrocellulose filter, Nitrocellulose filters, Northern blot analysis, Nucleic acids, Nucleotide, Other telomeres, Pcth5, Phage, Plasmid, Recombinant, Recombinant clones, Room temperature, Salivary glands, Telomere, Telomeric, Telomeric sequence, Telomeric sequences, Thummi, Thummi larvae.
Abstract
Abstract: In Chironomus thummi thummi a Balbiani ring-like structure is induced at the right end of chromosome III by heat shock. Telomere-specific clones were obtained by microcloning this chromosome region. One of the recombinant clones, λCthS, which had an insert of 176 base pairs (bp), hybridized in situ to all the chromosome ends of C. th. thummi except one on the acrocentric chromosome IV. Homologous sequences were also present at two interstitial sites on chromosome II. Southern analysis of genomic DNA indicated that the 176-bp insert represents a basic repeating unit clustered in tandem arrays. Around 1000 copies of this sequence were located in each telomere. The repeats were homogeneous in size but showed some sequence divergence, which seemed to increase when different telomeres were compared. At the nucleotide sequence level the most noticeable feature was the presence of tracts of adenine alternating with tracts of thymine. In situ hybridzation to chromosomal RNA showed that the 176-bp repeat was complementary to the RNA transcribed at the T-BRIII region, and occasionally, to the RNA transcribed at some of the other telomeres during heat shock. Northern blot analysis of total RNA showed that a transcript larger than 10 kilobase pairs (kb) and complementary to the teloeric insert was present in heat-shocked as well as in untreated cells. However, at most only a very weak RNA hybridization was seen at the telomeres of untreated animals.
Url:
DOI: 10.1007/BF00328462
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J." last="Carmona">M. J. Carmona</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Morcillo, G" sort="Morcillo, G" uniqKey="Morcillo G" first="G." last="Morcillo">G. Morcillo</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid</wicri:regionArea></affiliation><affiliation wicri:level="1"><mods:affiliation>Chemistry Department of the Universidad Nacional de Educación a Distancia, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Chemistry Department of the Universidad Nacional de Educación a Distancia</wicri:regionArea></affiliation></author><author><name sortKey="Galler, R" sort="Galler, R" uniqKey="Galler R" first="R." last="Galler">R. Galler</name><affiliation wicri:level="1"><mods:affiliation>European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg, Federal Republic of Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg</wicri:regionArea></affiliation></author><author><name sortKey="Martinez Salas, E" sort="Martinez Salas, E" uniqKey="Martinez Salas E" first="E." last="Martínez-Salas">E. Martínez-Salas</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid</wicri:regionArea></affiliation></author><author><name sortKey="De La Campa, A G" sort="De La Campa, A G" uniqKey="De La Campa A" first="A. G." last="De La Campa">A. G. De La Campa</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Diez, J L" sort="Diez, J L" uniqKey="Diez J" first="J. L." last="Díez">J. L. Díez</name><affiliation wicri:level="1"><mods:affiliation>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid, Spain</mods:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Cellular, Veláquez, 144, 28006, Madrid</wicri:regionArea></affiliation></author><author><name sortKey="Edstrom, J E" sort="Edstrom, J E" uniqKey="Edstrom J" first="J. E." last="Edström">J. E. Edström</name><affiliation wicri:level="1"><mods:affiliation>European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg, Federal Republic of Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Chromosoma</title><title level="j" type="abbrev">Chromosoma</title><idno type="ISSN">0009-5915</idno><idno type="eISSN">1432-0886</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1985-06-01">1985-06-01</date><biblScope unit="volume">92</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="108">108</biblScope><biblScope unit="page" to="115">115</biblScope></imprint><idno type="ISSN">0009-5915</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0009-5915</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Chironomus</term><term>Chironomus thummi</term><term>Chromosoma</term><term>Chromosomal</term><term>Chromosome</term><term>Chromosome ends</term><term>Clone</term><term>Digestion</term><term>Drosophila</term><term>Enzo biochem</term><term>Genome</term><term>Genomic</term><term>Heat shock</term><term>Hybridization</term><term>Hybridized</term><term>Larva</term><term>Molecular characterization</term><term>Morcillo</term><term>Nitrocellulose</term><term>Nitrocellulose filter</term><term>Nitrocellulose filters</term><term>Northern blot analysis</term><term>Nucleic acids</term><term>Nucleotide</term><term>Other telomeres</term><term>Pcth5</term><term>Phage</term><term>Plasmid</term><term>Recombinant</term><term>Recombinant clones</term><term>Room temperature</term><term>Salivary glands</term><term>Telomere</term><term>Telomeric</term><term>Telomeric sequence</term><term>Telomeric sequences</term><term>Thummi</term><term>Thummi larvae</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: In Chironomus thummi thummi a Balbiani ring-like structure is induced at the right end of chromosome III by heat shock. Telomere-specific clones were obtained by microcloning this chromosome region. One of the recombinant clones, λCthS, which had an insert of 176 base pairs (bp), hybridized in situ to all the chromosome ends of C. th. thummi except one on the acrocentric chromosome IV. Homologous sequences were also present at two interstitial sites on chromosome II. Southern analysis of genomic DNA indicated that the 176-bp insert represents a basic repeating unit clustered in tandem arrays. Around 1000 copies of this sequence were located in each telomere. The repeats were homogeneous in size but showed some sequence divergence, which seemed to increase when different telomeres were compared. At the nucleotide sequence level the most noticeable feature was the presence of tracts of adenine alternating with tracts of thymine. In situ hybridzation to chromosomal RNA showed that the 176-bp repeat was complementary to the RNA transcribed at the T-BRIII region, and occasionally, to the RNA transcribed at some of the other telomeres during heat shock. Northern blot analysis of total RNA showed that a transcript larger than 10 kilobase pairs (kb) and complementary to the teloeric insert was present in heat-shocked as well as in untreated cells. However, at most only a very weak RNA hybridization was seen at the telomeres of untreated animals.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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