Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase
Identifieur interne : 000254 ( Istex/Curation ); précédent : 000253; suivant : 000255Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase
Auteurs : Georges Maury [France] ; Abdelaziz El Alaoui [France] ; François Morvan [France] ; Barbara Muller [Allemagne] ; Jean-Louis Imbach [France] ; Roger S. Goody [Allemagne]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 1992.
English descriptors
- Teeft :
- Academic press, Aids research, Analog, Annealed, Biophysical, Biophysical research communications, Competitive inhibition, Dissociation constants, Duplex, Duplex poly, Experimental conditions, First stage, First step, Fluorescence experiments, Fluorescence measurements, Free enzyme, High concentrations, Human retrovirus, Hybrid, Inhibitor, Inhibitor concentrations, Inhibitor properties, Inhibitory properties, Kinetic constants, Nucleotide, Oligomers, Oligonucleotides, Phosphorothioate, Phosphorothioate oligonucleotides, Poly, Primer, Primer analogs, Steady state, Substrate properties, Transcriptase, Uncompetitive inhibition.
Abstract
Summary: We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of the enzyme using Poly(rA).(dT)14 as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase. The two methods gave the same relative order of affinitiy. Phosphorothioate oligodeoxyribonucleotides had a much higher affinity than oligo(dC)14 and it was increased on hybridization. Quantitatively similar results were obtained for S(dC)14 or its analog with bases in the a-configuration. Of the analogs tested, only S(dC)14 showed priming activity.
Url:
DOI: 10.1016/S0006-291X(05)81540-2
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000254
Links to Exploration step
ISTEX:D98A4CFC304410C8B59AE56F267084FA8AC99F7ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase</title><author><name sortKey="Maury, Georges" sort="Maury, Georges" uniqKey="Maury G" first="Georges" last="Maury">Georges Maury</name><affiliation wicri:level="1"><mods:affiliation>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5</wicri:regionArea></affiliation></author><author><name sortKey="Alaoui, Abdelaziz El" sort="Alaoui, Abdelaziz El" uniqKey="Alaoui A" first="Abdelaziz El" last="Alaoui">Abdelaziz El Alaoui</name><affiliation wicri:level="1"><mods:affiliation>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5</wicri:regionArea></affiliation></author><author><name sortKey="Morvan, Francois" sort="Morvan, Francois" uniqKey="Morvan F" first="François" last="Morvan">François Morvan</name><affiliation wicri:level="1"><mods:affiliation>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5</wicri:regionArea></affiliation></author><author><name sortKey="Muller, Barbara" sort="Muller, Barbara" uniqKey="Muller B" first="Barbara" last="Muller">Barbara Muller</name><affiliation wicri:level="1"><mods:affiliation>Abteilung Biophysik, Max Planck Institut für medizinische Forschung Jahnstrasse 29, 6900 Heidelberg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Abteilung Biophysik, Max Planck Institut für medizinische Forschung Jahnstrasse 29, 6900 Heidelberg</wicri:regionArea></affiliation></author><author><name sortKey="Imbach, Jean Louis" sort="Imbach, Jean Louis" uniqKey="Imbach J" first="Jean-Louis" last="Imbach">Jean-Louis Imbach</name><affiliation wicri:level="1"><mods:affiliation>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5</wicri:regionArea></affiliation></author><author><name sortKey="Goody, Roger S" sort="Goody, Roger S" uniqKey="Goody R" first="Roger S." last="Goody">Roger S. Goody</name><affiliation wicri:level="1"><mods:affiliation>Abteilung Biophysik, Max Planck Institut für medizinische Forschung Jahnstrasse 29, 6900 Heidelberg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Abteilung Biophysik, Max Planck Institut für medizinische Forschung Jahnstrasse 29, 6900 Heidelberg</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D98A4CFC304410C8B59AE56F267084FA8AC99F7A</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1016/S0006-291X(05)81540-2</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-P63RKWPQ-T/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000254</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000254</idno><idno type="wicri:Area/Istex/Curation">000254</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase</title><author><name sortKey="Maury, Georges" sort="Maury, Georges" uniqKey="Maury G" first="Georges" last="Maury">Georges Maury</name><affiliation wicri:level="1"><mods:affiliation>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5</wicri:regionArea></affiliation></author><author><name sortKey="Alaoui, Abdelaziz El" sort="Alaoui, Abdelaziz El" uniqKey="Alaoui A" first="Abdelaziz El" last="Alaoui">Abdelaziz El Alaoui</name><affiliation wicri:level="1"><mods:affiliation>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5</wicri:regionArea></affiliation></author><author><name sortKey="Morvan, Francois" sort="Morvan, Francois" uniqKey="Morvan F" first="François" last="Morvan">François Morvan</name><affiliation wicri:level="1"><mods:affiliation>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5</wicri:regionArea></affiliation></author><author><name sortKey="Muller, Barbara" sort="Muller, Barbara" uniqKey="Muller B" first="Barbara" last="Muller">Barbara Muller</name><affiliation wicri:level="1"><mods:affiliation>Abteilung Biophysik, Max Planck Institut für medizinische Forschung Jahnstrasse 29, 6900 Heidelberg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Abteilung Biophysik, Max Planck Institut für medizinische Forschung Jahnstrasse 29, 6900 Heidelberg</wicri:regionArea></affiliation></author><author><name sortKey="Imbach, Jean Louis" sort="Imbach, Jean Louis" uniqKey="Imbach J" first="Jean-Louis" last="Imbach">Jean-Louis Imbach</name><affiliation wicri:level="1"><mods:affiliation>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Montpellier II, URA 488 du CNRS, Département de Chimie Organique Fine, Place E. Bataillon, 34095 Montpellier Cédex 5</wicri:regionArea></affiliation></author><author><name sortKey="Goody, Roger S" sort="Goody, Roger S" uniqKey="Goody R" first="Roger S." last="Goody">Roger S. Goody</name><affiliation wicri:level="1"><mods:affiliation>Abteilung Biophysik, Max Planck Institut für medizinische Forschung Jahnstrasse 29, 6900 Heidelberg, Germany</mods:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Abteilung Biophysik, Max Planck Institut für medizinische Forschung Jahnstrasse 29, 6900 Heidelberg</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Biochemical and Biophysical Research Communications</title><title level="j" type="abbrev">YBBRC</title><idno type="ISSN">0006-291X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">186</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="1249">1249</biblScope><biblScope unit="page" to="1256">1256</biblScope></imprint><idno type="ISSN">0006-291X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-291X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Aids research</term><term>Analog</term><term>Annealed</term><term>Biophysical</term><term>Biophysical research communications</term><term>Competitive inhibition</term><term>Dissociation constants</term><term>Duplex</term><term>Duplex poly</term><term>Experimental conditions</term><term>First stage</term><term>First step</term><term>Fluorescence experiments</term><term>Fluorescence measurements</term><term>Free enzyme</term><term>High concentrations</term><term>Human retrovirus</term><term>Hybrid</term><term>Inhibitor</term><term>Inhibitor concentrations</term><term>Inhibitor properties</term><term>Inhibitory properties</term><term>Kinetic constants</term><term>Nucleotide</term><term>Oligomers</term><term>Oligonucleotides</term><term>Phosphorothioate</term><term>Phosphorothioate oligonucleotides</term><term>Poly</term><term>Primer</term><term>Primer analogs</term><term>Steady state</term><term>Substrate properties</term><term>Transcriptase</term><term>Uncompetitive inhibition</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: We have investigated the interaction between a number of 14 mers phosphorothioate oligonucleotides and HIV-1 reverse transcriptase. Two methods were used to measure the affinity of the analogs for the enzyme. In the first, the oligonucleotide or its duplex with Poly(rl) were used as inhibitors of the enzyme using Poly(rA).(dT)14 as template primer. In the second, the oligonucleotides or their duplexes were used to displace a fluorescent template.primer complex of known affinity from its binding site on reverse transcriptase. The two methods gave the same relative order of affinitiy. Phosphorothioate oligodeoxyribonucleotides had a much higher affinity than oligo(dC)14 and it was increased on hybridization. Quantitatively similar results were obtained for S(dC)14 or its analog with bases in the a-configuration. Of the analogs tested, only S(dC)14 showed priming activity.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000254 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000254 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:D98A4CFC304410C8B59AE56F267084FA8AC99F7A
|texte= Template. Phosphorothioate oligonucleotides duplexes as inhibitors of HIV-1 reverse transcriptase
}}
| This area was generated with Dilib version V0.6.33. |  |