In vitro formation of flagella-like filaments and other structures from flagellin
Identifieur interne : 002585 ( Istex/Corpus ); précédent : 002584; suivant : 002586In vitro formation of flagella-like filaments and other structures from flagellin
Auteurs : Dinah Abram ; Henry KofflerSource :
- Journal of Molecular Biology [ 0022-2836 ] ; 1964.
English descriptors
- Teeft :
- Abram, Acta, Acta chem, Ambler rees, Aqueous solution, Aqueous suspensions, Bact, Biol, Biophys, Borotungstate, Cell weight, Centrifugation, Certain conditions, Chem, Concentration offlagellin, Deionized water, Electron microscopy, Filament, Final concentration, Flagellalike filaments, Flagellar, Flagellar suspensions, Flagellin, Flagellin aggregates, Flagellin concentration, Flagellin molecules, Flagellin monomers, Flagellin reaggregated, Flagellin reaggregates, Flagellin solutions, Flagellins, Flagellum, High concentrations, Higher concentrations, Higher temperatures, Ionic strength, Kerridge, Koffler, Material reaggregates, Mgjml, Microbiol, Monomer, Native flagella, Other hand, Pellet, Phosphate buffer, Phosphotungstate, Plate viii, Polymer, Potassium phosphotungstate, Proc, Purdue university, Reaction mixture, Reaction mixtures, Reaggregated, Reaggregated material, Reaggregates, Reaggregation, Reaggregation reaction, Reconstituted, Short filaments, Similar experiments, Spinco model, Stenesh, Straight aggregates, Straight filaments, Straight reaggregates, Straight structures, Subunit, Uranyl, Ures, Viscous, Weibull, Weibull tiselius.
Abstract
The in vitro reaggregation of flagellin monomers, prepared by acid disintegration of isolated native flagella of Bacillus pumilus, was studied. Flagellin reassembles in at least three forms, two of which are highly organized in a linear fashion, and appear as straight structures and flagella-like filaments. The latter closely resemble native flagella in their morphology and in other properties. The rate and the extent of the reaggregation reaction, and the structure of the aggregates produced, are affected by the concentration of flagellin, pH, salt and temperature. Reaggregation at 2°C and in frozen samples makes it unlikely that the reaction is enzymic. Straight aggregates can be transformed to flagella-like filaments if they are suspended at an appropriate pH. On the other hand, neither flagella-like filaments nor native flagella can be changed to the straight forms by incubation at a pH at which the latter assemble from monomers. The results indicate that the primary structure of the flagellin molecules determines their configuration assumed under various conditions and the manner in which they reassemble to larger polymers in a specific environment. Flagella-like filaments and probably native flagella as well constitute the most stable organized state in which flagellin can aggregate.
Url:
DOI: 10.1016/S0022-2836(64)80098-X
Links to Exploration step
ISTEX:E72AC8EB0D4DBF9ADBAEA32B2885F80F0BF00390Le document en format XML
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Flagellin reassembles in at least three forms, two of which are highly organized in a linear fashion, and appear as straight structures and flagella-like filaments. The latter closely resemble native flagella in their morphology and in other properties. The rate and the extent of the reaggregation reaction, and the structure of the aggregates produced, are affected by the concentration of flagellin, pH, salt and temperature. Reaggregation at 2°C and in frozen samples makes it unlikely that the reaction is enzymic. Straight aggregates can be transformed to flagella-like filaments if they are suspended at an appropriate pH. On the other hand, neither flagella-like filaments nor native flagella can be changed to the straight forms by incubation at a pH at which the latter assemble from monomers. The results indicate that the primary structure of the flagellin molecules determines their configuration assumed under various conditions and the manner in which they reassemble to larger polymers in a specific environment. 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Flagellin reassembles in at least three forms, two of which are highly organized in a linear fashion, and appear as straight structures and flagella-like filaments. The latter closely resemble native flagella in their morphology and in other properties. The rate and the extent of the reaggregation reaction, and the structure of the aggregates produced, are affected by the concentration of flagellin, pH, salt and temperature. Reaggregation at 2°C and in frozen samples makes it unlikely that the reaction is enzymic. Straight aggregates can be transformed to flagella-like filaments if they are suspended at an appropriate pH. On the other hand, neither flagella-like filaments nor native flagella can be changed to the straight forms by incubation at a pH at which the latter assemble from monomers. The results indicate that the primary structure of the flagellin molecules determines their configuration assumed under various conditions and the manner in which they reassemble to larger polymers in a specific environment. Flagella-like filaments and probably native flagella as well constitute the most stable organized state in which flagellin can aggregate.</abstract><qualityIndicators><score>9.352</score><pdfWordCount>9523</pdfWordCount><pdfCharCount>53330</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>33</pdfPageCount><pdfPageSize>414 x 656.64 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>196</abstractWordCount><abstractCharCount>1310</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>In vitro formation of flagella-like filaments and other structures from flagellin</title><pmid><json:string>14200380</json:string></pmid><pii><json:string>S0022-2836(64)80098-X</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Journal of Molecular Biology</title><language><json:string>unknown</json:string></language><publicationDate>1964</publicationDate><issn><json:string>0022-2836</json:string></issn><pii><json:string>S0022-2836(64)X8085-6</json:string></pii><volume>9</volume><issue>1</issue><pages><first>168</first><last>185</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>80S</json:string><json:string>3300 to 6000</json:string><json:string>1964</json:string></date><geogName></geogName><orgName><json:string>Biochem</json:string><json:string>Brandeis University</json:string><json:string>NSF grant G-24237</json:string><json:string>NIH grant AI-00685</json:string></orgName><orgName_funder><json:string>NSF grant G-24237</json:string><json:string>NIH grant AI-00685</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Florence Shen</json:string><json:string>I. 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(1955)</json:string><json:string>Koffier, Mallett & Adye, 1957</json:string><json:string>Read, 1957</json:string><json:string>Koffler et al., 1957</json:string><json:string>Vennes & Gerhardt, 1959</json:string><json:string>Takahashi & Ishii, 1952</json:string><json:string>Hodge, 1959</json:string><json:string>Straub & Feuer, 1950</json:string><json:string>Nasser, 1964</json:string><json:string>Stocker et al., 1953</json:string><json:string>Weinstein, Koffler & Moskowitz, 1960</json:string><json:string>Koffler, 1957</json:string><json:string>Kerridge et al., 1962</json:string><json:string>Kobayashi, Rinker & Komer, 1959</json:string><json:string>Lowry, Rosebrough, Farr & Randall, 1951</json:string><json:string>Stenesh & Koffier, 1962</json:string><json:string>Ambler & Rees, 1959</json:string><json:string>Martinez, 1963</json:string><json:string>Laki, Bowen & Clark, 1950</json:string><json:string>Quadling & Stocker, 1956</json:string><json:string>Kobayashi et al., 1959</json:string><json:string>Beighton, Porter & Stocker, 1958</json:string><json:string>Friewer & Leifson, 1952</json:string><json:string>Komer, 1957</json:string><json:string>Hirsch, 1947</json:string><json:string>Ada et al., 1963</json:string><json:string>Kerridge, 1960</json:string><json:string>Deal, Rutter & Van Holde, 1963</json:string><json:string>Koffier, 1957</json:string><json:string>Burge, 1961</json:string><json:string>Yaguchi et al., 1964</json:string><json:string>Weibull & 'I'iselius, 1945</json:string><json:string>Levinthal, Singer & Fetherolf, 1962</json:string><json:string>Markert, 1963</json:string><json:string>Abram et al.</json:string><json:string>Weinstein, 1959</json:string><json:string>Preston & Maitland, 1952</json:string><json:string>Astbury, Beighton & Weibull, 1955</json:string><json:string>Kobayashi et al. (1959)</json:string><json:string>Stellwagen & Schachman, 1962</json:string><json:string>Fischer, 1963</json:string><json:string>Brenner & Horne, 1959</json:string><json:string>Nasser & Koffler, 1963</json:string><json:string>Ada et al. (1963)</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-FTTFTR49-0</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - biochemistry & molecular biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Molecular Biology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1964</publicationDate><copyrightDate>1964</copyrightDate><doi><json:string>10.1016/S0022-2836(64)80098-X</json:string></doi><id>E72AC8EB0D4DBF9ADBAEA32B2885F80F0BF00390</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FTTFTR49-0/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FTTFTR49-0/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-FTTFTR49-0/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">In vitro formation of flagella-like filaments and other structures from flagellin</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1964 Academic Press Inc. 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Flagellin reassembles in at least three forms, two of which are highly organized in a linear fashion, and appear as straight structures and flagella-like filaments. The latter closely resemble native flagella in their morphology and in other properties. The rate and the extent of the reaggregation reaction, and the structure of the aggregates produced, are affected by the concentration of flagellin, pH, salt and temperature. Reaggregation at 2°C and in frozen samples makes it unlikely that the reaction is enzymic. Straight aggregates can be transformed to flagella-like filaments if they are suspended at an appropriate pH. On the other hand, neither flagella-like filaments nor native flagella can be changed to the straight forms by incubation at a pH at which the latter assemble from monomers. The results indicate that the primary structure of the flagellin molecules determines their configuration assumed under various conditions and the manner in which they reassemble to larger polymers in a specific environment. Flagella-like filaments and probably native flagella as well constitute the most stable organized state in which flagellin can aggregate.</p></abstract></profileDesc><revisionDesc><change when="1964-03-23">Modified</change><change when="1964">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FTTFTR49-0/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="fla"><item-info><jid>YJMBI</jid><aid>80098</aid><ce:pii>S0022-2836(64)80098-X</ce:pii><ce:doi>10.1016/S0022-2836(64)80098-X</ce:doi><ce:copyright type="unknown" year="1964">Academic Press Inc. (London) Ltd.</ce:copyright></item-info><head><ce:article-footnote><ce:label>*</ce:label><ce:note-para>This work was supported in part by NIH grant AI-00685 and NSF grant G-24237.</ce:note-para></ce:article-footnote><ce:title><ce:italic>In vitro</ce:italic> formation of flagella-like filaments and other structures from flagellin</ce:title><ce:author-group><ce:author><ce:given-name>Dinah</ce:given-name><ce:surname>Abram</ce:surname></ce:author><ce:author><ce:given-name>Henry</ce:given-name><ce:surname>Koffler</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Biological Sciences, Purdue University, Lafayette, Indiana, U.S.A.</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="11" month="2" year="1964"></ce:date-received><ce:date-revised day="23" month="3" year="1964"></ce:date-revised><ce:abstract id="ab1"><ce:abstract-sec><ce:simple-para>The <ce:italic>in vitro</ce:italic> reaggregation of flagellin monomers, prepared by acid disintegration of isolated native flagella of <ce:italic>Bacillus pumilus</ce:italic>, was studied. Flagellin reassembles in at least three forms, two of which are highly organized in a linear fashion, and appear as straight structures and flagella-like filaments. The latter closely resemble native flagella in their morphology and in other properties. The rate and the extent of the reaggregation reaction, and the structure of the aggregates produced, are affected by the concentration of flagellin, pH, salt and temperature. Reaggregation at 2°C and in frozen samples makes it unlikely that the reaction is enzymic. Straight aggregates can be transformed to flagella-like filaments if they are suspended at an appropriate pH. On the other hand, neither flagella-like filaments nor native flagella can be changed to the straight forms by incubation at a pH at which the latter assemble from monomers. The results indicate that the primary structure of the flagellin molecules determines their configuration assumed under various conditions and the manner in which they reassemble to larger polymers in a specific environment. Flagella-like filaments and probably native flagella as well constitute the most stable organized state in which flagellin can aggregate.</ce:simple-para></ce:abstract-sec></ce:abstract></head><tail><ce:bibliography><ce:section-title>References</ce:section-title><ce:bibliography-sec><ce:bib-reference id="bib1"><ce:label>Abram and Koffler, 1963</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:given-name>D.</ce:given-name><ce:surname>Abram</ce:surname></sb:author><sb:author><ce:given-name>H.</ce:given-name><ce:surname>Koffler</ce:surname></sb:author></sb:authors></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Bact. 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Bact.</sb:maintitle></sb:title><sb:volume-nr>81</sb:volume-nr></sb:series><sb:date>1961</sb:date></sb:issue><sb:pages><sb:first-page>195</sb:first-page></sb:pages></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>In vitro formation of flagella-like filaments and other structures from flagellin</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>formation of flagella-like filaments and other structures from flagellin</title></titleInfo><name type="personal"><namePart type="given">Dinah</namePart><namePart type="family">Abram</namePart><affiliation>Department of Biological Sciences, Purdue University, Lafayette, Indiana, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Henry</namePart><namePart type="family">Koffler</namePart><affiliation>Department of Biological Sciences, Purdue University, Lafayette, Indiana, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1964</dateIssued><dateModified encoding="w3cdtf">1964-03-23</dateModified><copyrightDate encoding="w3cdtf">1964</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract>The in vitro reaggregation of flagellin monomers, prepared by acid disintegration of isolated native flagella of Bacillus pumilus, was studied. Flagellin reassembles in at least three forms, two of which are highly organized in a linear fashion, and appear as straight structures and flagella-like filaments. The latter closely resemble native flagella in their morphology and in other properties. The rate and the extent of the reaggregation reaction, and the structure of the aggregates produced, are affected by the concentration of flagellin, pH, salt and temperature. Reaggregation at 2°C and in frozen samples makes it unlikely that the reaction is enzymic. Straight aggregates can be transformed to flagella-like filaments if they are suspended at an appropriate pH. On the other hand, neither flagella-like filaments nor native flagella can be changed to the straight forms by incubation at a pH at which the latter assemble from monomers. The results indicate that the primary structure of the flagellin molecules determines their configuration assumed under various conditions and the manner in which they reassemble to larger polymers in a specific environment. Flagella-like filaments and probably native flagella as well constitute the most stable organized state in which flagellin can aggregate.</abstract><note>This work was supported in part by NIH grant AI-00685 and NSF grant G-24237.</note><relatedItem type="host"><titleInfo><title>Journal of Molecular Biology</title></titleInfo><titleInfo type="abbreviated"><title>YJMBI</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1964</dateIssued></originInfo><identifier type="ISSN">0022-2836</identifier><identifier type="PII">S0022-2836(64)X8085-6</identifier><part><date>1964</date><detail type="volume"><number>9</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>268</end></extent><extent unit="pages"><start>168</start><end>185</end></extent></part></relatedItem><identifier type="istex">E72AC8EB0D4DBF9ADBAEA32B2885F80F0BF00390</identifier><identifier type="ark">ark:/67375/6H6-FTTFTR49-0</identifier><identifier type="DOI">10.1016/S0022-2836(64)80098-X</identifier><identifier type="PII">S0022-2836(64)80098-X</identifier><identifier type="ArticleID">80098</identifier><accessCondition type="use and reproduction" contentType="copyright">©1964 Academic Press Inc. (London) Ltd.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press Inc. (London) Ltd., ©1964</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-FTTFTR49-0/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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