Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses
Identifieur interne : 002541 ( Istex/Corpus ); précédent : 002540; suivant : 002542Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses
Auteurs : J. Blok ; S. M. Mcwilliam ; H. C. Butler ; A. J. Gibbs ; G. Weiller ; B. L. Herring ; A. C. Hemsley ; J. G. Aaskov ; S. Yoksan ; N. BhamarapravatiSource :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Academic press, Acid, Amino, Amino acid changes, Amino acid differences, Amino acid residues, Amino acid sequence, Amino acid sequences, Amino acid substitutions, Amino acids, Asibi, Attenuated, Avirulent, Biol, Black beetle nodavirus, Blok, Bruenn, Cdna, Cdna clones, Cdna synthesis, Clone, Comparative analysis, Complete nucleotide sequence, D2pdk, D2sl, Dendrogram, Dendrograms, Dengue, Dengue serotypes, Dengue type, Dengue virus, Dengue viruses, Distance measures, Dpjam, Dpngc, Encephalitis, Encephalitis virus, Feng, Flavivirus, Flavivirus proteins, Flavivirus tree, Flavivirus west nile virus, Full names, Genes coding, Genetic recombination, Genome, Glycoprotein, Immunization, Japanese encephalitis, Mandl, Monoclonal, Monoclonal antibodies, Mosaic, Murray valley encephalitis, Nonstructural, Nonstructural proteins, Nonvirion proteins, Ns2a, Ns2b, Ns4a, Ns4b, Nucleic, Nucleic acids, Nucleotide, Nucleotide changes, Nucleotide differences, Nucleotide sequence, Nucleotide sequences, Oligonucleotide, Other flaviviruses, Other viruses, Partial nucleotide sequence, Polymerase, Positive strand, Primer, Proc, Protein, Protein sequences, Room temperature, Scale divisions, Scale units, Schlesinger, Sequence analysis, Sequence data, Sequencing, Sister group, Structural proteins, Substitution, Swissprot database, Tree method, Trent, Vaccine, Viral, Virion, Virion proteins, Virology, Virulent, Virus, Wengler, Yellow fever, Yellow fever virus, Yoksan.
Abstract
Abstract: A comparison of the sequence of the dengue-2 16681 virus with that of the candidate vaccine strain (16681-PDK53) derived from it identified 53 of the 10,723 nucleotides which differed between the strains. Nucleotide changes occurred in genes coding for all virion and nonvirion proteins, and in the 5′ and 3′ untranslated regions. Twenty-seven of the nucleotide changes resulted in amino acid alterations. The greatest amino acid sequence differences in the virion proteins occurred in prM (2.20%; 291 amino acids) followed by the M protein (1.33%; 175 amino acids), the C protein (0.88%; 1114 amino acid), and the E protein (0.61%; 3495 amino acids). Differences in the amino acid sequence of nonvirion proteins ranged from 1.51% (6398 amino acids) in NS4 to 0.33% (3900 amino acids) in NS5. The encoded protein sequences of 16681-PDK53 were also compared with the published sequences of other flaviviruses to obtain a detailed classification of 17 flaviviruses using the neighbor-joining tree method. The analyses of the sequence data produced dendrograms which supported the traditional groupings based on serological evidence, and they suggested that the flaviviruses have evolved by divergent mutational change and there was no evidence of genetic recombination between members of the group. Comparisons of the sequences of the flavivirus polymerase and helicase-like proteins (NS5 and NS3, respectively) with those from other viruses yielded a classification of the flaviviruses indicating that the primary division of the flaviviruses was between those transmitted by mosquitoes and those transmitted by ticks.
Url:
DOI: 10.1016/0042-6822(92)90460-7
Links to Exploration step
ISTEX:4D6F87B101DF40A6F8C49821E40BEE30F0577DE3Le document en format XML
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Blok</name><affiliation><mods:affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</mods:affiliation></affiliation><affiliation><mods:affiliation>To whom correspondence and reprint requests should be addressed.</mods:affiliation></affiliation></author><author><name sortKey="Mcwilliam, S M" sort="Mcwilliam, S M" uniqKey="Mcwilliam S" first="S. M." last="Mcwilliam">S. M. Mcwilliam</name><affiliation><mods:affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</mods:affiliation></affiliation><affiliation><mods:affiliation>3Present address: CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Brisbane, Queensland, 4068, Australia.</mods:affiliation></affiliation></author><author><name sortKey="Butler, H C" sort="Butler, H C" uniqKey="Butler H" first="H. C." last="Butler">H. C. Butler</name><affiliation><mods:affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</mods:affiliation></affiliation></author><author><name sortKey="Gibbs, A J" sort="Gibbs, A J" uniqKey="Gibbs A" first="A. J." last="Gibbs">A. J. Gibbs</name><affiliation><mods:affiliation>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</mods:affiliation></affiliation></author><author><name sortKey="Weiller, G" sort="Weiller, G" uniqKey="Weiller G" first="G." last="Weiller">G. Weiller</name><affiliation><mods:affiliation>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</mods:affiliation></affiliation></author><author><name sortKey="Herring, B L" sort="Herring, B L" uniqKey="Herring B" first="B. L." last="Herring">B. L. 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Yoksan</name><affiliation><mods:affiliation>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</mods:affiliation></affiliation></author><author><name sortKey="Bhamarapravati, N" sort="Bhamarapravati, N" uniqKey="Bhamarapravati N" first="N." last="Bhamarapravati">N. Bhamarapravati</name><affiliation><mods:affiliation>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992">1992</date><biblScope unit="volume">187</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="573">573</biblScope><biblScope unit="page" to="590">590</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Acid</term><term>Amino</term><term>Amino acid changes</term><term>Amino acid differences</term><term>Amino acid residues</term><term>Amino acid sequence</term><term>Amino acid sequences</term><term>Amino acid substitutions</term><term>Amino acids</term><term>Asibi</term><term>Attenuated</term><term>Avirulent</term><term>Biol</term><term>Black beetle nodavirus</term><term>Blok</term><term>Bruenn</term><term>Cdna</term><term>Cdna clones</term><term>Cdna synthesis</term><term>Clone</term><term>Comparative analysis</term><term>Complete nucleotide sequence</term><term>D2pdk</term><term>D2sl</term><term>Dendrogram</term><term>Dendrograms</term><term>Dengue</term><term>Dengue serotypes</term><term>Dengue type</term><term>Dengue virus</term><term>Dengue viruses</term><term>Distance measures</term><term>Dpjam</term><term>Dpngc</term><term>Encephalitis</term><term>Encephalitis virus</term><term>Feng</term><term>Flavivirus</term><term>Flavivirus proteins</term><term>Flavivirus tree</term><term>Flavivirus west nile virus</term><term>Full names</term><term>Genes coding</term><term>Genetic recombination</term><term>Genome</term><term>Glycoprotein</term><term>Immunization</term><term>Japanese encephalitis</term><term>Mandl</term><term>Monoclonal</term><term>Monoclonal antibodies</term><term>Mosaic</term><term>Murray valley encephalitis</term><term>Nonstructural</term><term>Nonstructural proteins</term><term>Nonvirion proteins</term><term>Ns2a</term><term>Ns2b</term><term>Ns4a</term><term>Ns4b</term><term>Nucleic</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide changes</term><term>Nucleotide differences</term><term>Nucleotide sequence</term><term>Nucleotide sequences</term><term>Oligonucleotide</term><term>Other flaviviruses</term><term>Other viruses</term><term>Partial nucleotide sequence</term><term>Polymerase</term><term>Positive strand</term><term>Primer</term><term>Proc</term><term>Protein</term><term>Protein sequences</term><term>Room temperature</term><term>Scale divisions</term><term>Scale units</term><term>Schlesinger</term><term>Sequence analysis</term><term>Sequence data</term><term>Sequencing</term><term>Sister group</term><term>Structural proteins</term><term>Substitution</term><term>Swissprot database</term><term>Tree method</term><term>Trent</term><term>Vaccine</term><term>Viral</term><term>Virion</term><term>Virion proteins</term><term>Virology</term><term>Virulent</term><term>Virus</term><term>Wengler</term><term>Yellow fever</term><term>Yellow fever virus</term><term>Yoksan</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A comparison of the sequence of the dengue-2 16681 virus with that of the candidate vaccine strain (16681-PDK53) derived from it identified 53 of the 10,723 nucleotides which differed between the strains. Nucleotide changes occurred in genes coding for all virion and nonvirion proteins, and in the 5′ and 3′ untranslated regions. Twenty-seven of the nucleotide changes resulted in amino acid alterations. The greatest amino acid sequence differences in the virion proteins occurred in prM (2.20%; 291 amino acids) followed by the M protein (1.33%; 175 amino acids), the C protein (0.88%; 1114 amino acid), and the E protein (0.61%; 3495 amino acids). Differences in the amino acid sequence of nonvirion proteins ranged from 1.51% (6398 amino acids) in NS4 to 0.33% (3900 amino acids) in NS5. The encoded protein sequences of 16681-PDK53 were also compared with the published sequences of other flaviviruses to obtain a detailed classification of 17 flaviviruses using the neighbor-joining tree method. The analyses of the sequence data produced dendrograms which supported the traditional groupings based on serological evidence, and they suggested that the flaviviruses have evolved by divergent mutational change and there was no evidence of genetic recombination between members of the group. Comparisons of the sequences of the flavivirus polymerase and helicase-like proteins (NS5 and NS3, respectively) with those from other viruses yielded a classification of the flaviviruses indicating that the primary division of the flaviviruses was between those transmitted by mosquitoes and those transmitted by ticks.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>flavivirus</json:string><json:string>dengue</json:string><json:string>viral</json:string><json:string>genome</json:string><json:string>amino</json:string><json:string>cdna</json:string><json:string>virology</json:string><json:string>polymerase</json:string><json:string>dendrogram</json:string><json:string>sequencing</json:string><json:string>primer</json:string><json:string>asibi</json:string><json:string>wengler</json:string><json:string>dendrograms</json:string><json:string>clone</json:string><json:string>nucleotide</json:string><json:string>nucleic acids</json:string><json:string>encephalitis</json:string><json:string>blok</json:string><json:string>amino acid differences</json:string><json:string>nucleotide sequence</json:string><json:string>virion</json:string><json:string>other flaviviruses</json:string><json:string>ns4a</json:string><json:string>nonstructural</json:string><json:string>monoclonal</json:string><json:string>mandl</json:string><json:string>ns2a</json:string><json:string>d2sl</json:string><json:string>glycoprotein</json:string><json:string>ns4b</json:string><json:string>monoclonal antibodies</json:string><json:string>attenuated</json:string><json:string>feng</json:string><json:string>vaccine</json:string><json:string>trent</json:string><json:string>amino acids</json:string><json:string>virulent</json:string><json:string>proc</json:string><json:string>d2pdk</json:string><json:string>biol</json:string><json:string>amino acid substitutions</json:string><json:string>ns2b</json:string><json:string>bruenn</json:string><json:string>oligonucleotide</json:string><json:string>avirulent</json:string><json:string>dpjam</json:string><json:string>yoksan</json:string><json:string>dpngc</json:string><json:string>virus</json:string><json:string>nucleic</json:string><json:string>structural proteins</json:string><json:string>dengue type</json:string><json:string>dengue viruses</json:string><json:string>amino acid sequence</json:string><json:string>nucleotide changes</json:string><json:string>yellow fever virus</json:string><json:string>tree method</json:string><json:string>other viruses</json:string><json:string>amino acid sequences</json:string><json:string>cdna synthesis</json:string><json:string>cdna clones</json:string><json:string>yellow fever</json:string><json:string>amino acid changes</json:string><json:string>encephalitis virus</json:string><json:string>sequence data</json:string><json:string>virion proteins</json:string><json:string>nonstructural proteins</json:string><json:string>nucleotide differences</json:string><json:string>flavivirus proteins</json:string><json:string>distance measures</json:string><json:string>substitution</json:string><json:string>immunization</json:string><json:string>protein</json:string><json:string>amino acid residues</json:string><json:string>full names</json:string><json:string>scale units</json:string><json:string>room temperature</json:string><json:string>flavivirus tree</json:string><json:string>sister group</json:string><json:string>black beetle nodavirus</json:string><json:string>murray valley encephalitis</json:string><json:string>protein sequences</json:string><json:string>japanese encephalitis</json:string><json:string>nucleotide sequences</json:string><json:string>genes coding</json:string><json:string>complete nucleotide sequence</json:string><json:string>dengue virus</json:string><json:string>positive strand</json:string><json:string>sequence analysis</json:string><json:string>flavivirus west nile virus</json:string><json:string>swissprot database</json:string><json:string>dengue serotypes</json:string><json:string>scale divisions</json:string><json:string>academic press</json:string><json:string>partial nucleotide sequence</json:string><json:string>nonvirion proteins</json:string><json:string>genetic recombination</json:string><json:string>comparative analysis</json:string><json:string>acid</json:string><json:string>schlesinger</json:string><json:string>mosaic</json:string></teeft></keywords><author><json:item><name>J. Blok</name><affiliations><json:string>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</json:string><json:string>To whom correspondence and reprint requests should be addressed.</json:string></affiliations></json:item><json:item><name>S.M. McWilliam</name><affiliations><json:string>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</json:string><json:string>3Present address: CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Brisbane, Queensland, 4068, Australia.</json:string></affiliations></json:item><json:item><name>H.C. Butler</name><affiliations><json:string>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</json:string></affiliations></json:item><json:item><name>A.J. Gibbs</name><affiliations><json:string>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</json:string></affiliations></json:item><json:item><name>G. Weiller</name><affiliations><json:string>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</json:string></affiliations></json:item><json:item><name>B.L. Herring</name><affiliations><json:string>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</json:string></affiliations></json:item><json:item><name>A.C. Hemsley</name><affiliations><json:string>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</json:string></affiliations></json:item><json:item><name>J.G. Aaskov</name><affiliations><json:string>Queensland University of Technology, George St., Brisbane, Queensland 4000, Australia</json:string></affiliations></json:item><json:item><name>S. Yoksan</name><affiliations><json:string>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</json:string></affiliations></json:item><json:item><name>N. Bhamarapravati</name><affiliations><json:string>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-RJQPJ5N5-N</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A comparison of the sequence of the dengue-2 16681 virus with that of the candidate vaccine strain (16681-PDK53) derived from it identified 53 of the 10,723 nucleotides which differed between the strains. Nucleotide changes occurred in genes coding for all virion and nonvirion proteins, and in the 5′ and 3′ untranslated regions. Twenty-seven of the nucleotide changes resulted in amino acid alterations. The greatest amino acid sequence differences in the virion proteins occurred in prM (2.20%; 291 amino acids) followed by the M protein (1.33%; 175 amino acids), the C protein (0.88%; 1114 amino acid), and the E protein (0.61%; 3495 amino acids). Differences in the amino acid sequence of nonvirion proteins ranged from 1.51% (6398 amino acids) in NS4 to 0.33% (3900 amino acids) in NS5. The encoded protein sequences of 16681-PDK53 were also compared with the published sequences of other flaviviruses to obtain a detailed classification of 17 flaviviruses using the neighbor-joining tree method. The analyses of the sequence data produced dendrograms which supported the traditional groupings based on serological evidence, and they suggested that the flaviviruses have evolved by divergent mutational change and there was no evidence of genetic recombination between members of the group. Comparisons of the sequences of the flavivirus polymerase and helicase-like proteins (NS5 and NS3, respectively) with those from other viruses yielded a classification of the flaviviruses indicating that the primary division of the flaviviruses was between those transmitted by mosquitoes and those transmitted by ticks.</abstract><qualityIndicators><score>9.928</score><pdfWordCount>8942</pdfWordCount><pdfCharCount>64255</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>18</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>244</abstractWordCount><abstractCharCount>1627</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses</title><pmid><json:string>1312269</json:string></pmid><pii><json:string>0042-6822(92)90460-7</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Nad. Aced. Sci. USA</title><language><json:string>unknown</json:string></language><volume>87</volume><pages><first>8898</first><last>8902</last></pages></serie><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1992</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0441-2</json:string></pii><volume>187</volume><issue>2</issue><pages><first>573</first><last>590</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1992</json:string><json:string>1964</json:string><json:string>1668</json:string><json:string>19s</json:string></date><geogName></geogName><orgName><json:string>Academic Press, Inc</json:string><json:string>University of Technology</json:string><json:string>Mahidol University</json:string><json:string>Research School of Biological Sciences, Australian National University, Canberra City, ACT</json:string><json:string>University of Queensland</json:string><json:string>Long Pocket Laboratories, Brisbane</json:string><json:string>Thailand Received September</json:string><json:string>Division of Tropical Animal Production</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>George St</json:string><json:string>S. M. McWILLIAM</json:string><json:string>Saran Wrap</json:string></persName><placeName><json:string>Nile</json:string><json:string>Brisbane</json:string><json:string>Thailand</json:string><json:string>Australia</json:string><json:string>Jamaica</json:string><json:string>Bangkok</json:string><json:string>Mannheim</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Sanger et al., 1977</json:string><json:string>Yoksan et al., 1986</json:string><json:string>Halstead and Simasthien, 1970</json:string><json:string>Blok et al., 1984</json:string><json:string>Clarke and Cassals (1958)</json:string><json:string>Saitou and Nei, 1987</json:string><json:string>Feng and Doolittle, 1987, 1990</json:string><json:string>Halstead, 1981</json:string><json:string>Rosen, 1977</json:string><json:string>Schlesinger, 1977</json:string><json:string>Deubel et al., 1988</json:string><json:string>Speight et al., 1988</json:string><json:string>Bhamarapravati et al</json:string><json:string>Okayama and Berg (1982)</json:string><json:string>November 1990</json:string><json:string>Studier and Keppler, 1988</json:string><json:string>Maniatis et al., 1982</json:string><json:string>Air, 1979</json:string><json:string>Igarashi, 1978</json:string><json:string>Westaway et al., 1985</json:string><json:string>Devereux et al., 1984</json:string><json:string>Wengler and Wengler, 1981</json:string><json:string>Rice et al, (1986)</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-RJQPJ5N5-N</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - microbiologie</json:string></inist></categories><publicationDate>1992</publicationDate><copyrightDate>1992</copyrightDate><doi><json:string>10.1016/0042-6822(92)90460-7</json:string></doi><id>4D6F87B101DF40A6F8C49821E40BEE30F0577DE3</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RJQPJ5N5-N/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RJQPJ5N5-N/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-RJQPJ5N5-N/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1992</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. M85258 and M85259</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses</title><author xml:id="author-0000"><persName><forename type="first">J.</forename><surname>Blok</surname></persName><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation><affiliation>To whom correspondence and reprint requests should be addressed.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">S.M.</forename><surname>McWilliam</surname></persName><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation><affiliation>3Present address: CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Brisbane, Queensland, 4068, Australia.</affiliation></author><author xml:id="author-0002"><persName><forename type="first">H.C.</forename><surname>Butler</surname></persName><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation></author><author xml:id="author-0003"><persName><forename type="first">A.J.</forename><surname>Gibbs</surname></persName><affiliation>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</affiliation></author><author xml:id="author-0004"><persName><forename type="first">G.</forename><surname>Weiller</surname></persName><affiliation>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</affiliation></author><author xml:id="author-0005"><persName><forename type="first">B.L.</forename><surname>Herring</surname></persName><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation></author><author xml:id="author-0006"><persName><forename type="first">A.C.</forename><surname>Hemsley</surname></persName><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation></author><author xml:id="author-0007"><persName><forename type="first">J.G.</forename><surname>Aaskov</surname></persName><affiliation>Queensland University of Technology, George St., Brisbane, Queensland 4000, Australia</affiliation></author><author xml:id="author-0008"><persName><forename type="first">S.</forename><surname>Yoksan</surname></persName><affiliation>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</affiliation></author><author xml:id="author-0009"><persName><forename type="first">N.</forename><surname>Bhamarapravati</surname></persName><affiliation>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</affiliation></author><idno type="istex">4D6F87B101DF40A6F8C49821E40BEE30F0577DE3</idno><idno type="ark">ark:/67375/6H6-RJQPJ5N5-N</idno><idno type="DOI">10.1016/0042-6822(92)90460-7</idno><idno type="PII">0042-6822(92)90460-7</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0441-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992"></date><biblScope unit="volume">187</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="573">573</biblScope><biblScope unit="page" to="590">590</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1992</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A comparison of the sequence of the dengue-2 16681 virus with that of the candidate vaccine strain (16681-PDK53) derived from it identified 53 of the 10,723 nucleotides which differed between the strains. Nucleotide changes occurred in genes coding for all virion and nonvirion proteins, and in the 5′ and 3′ untranslated regions. Twenty-seven of the nucleotide changes resulted in amino acid alterations. The greatest amino acid sequence differences in the virion proteins occurred in prM (2.20%; 291 amino acids) followed by the M protein (1.33%; 175 amino acids), the C protein (0.88%; 1114 amino acid), and the E protein (0.61%; 3495 amino acids). Differences in the amino acid sequence of nonvirion proteins ranged from 1.51% (6398 amino acids) in NS4 to 0.33% (3900 amino acids) in NS5. The encoded protein sequences of 16681-PDK53 were also compared with the published sequences of other flaviviruses to obtain a detailed classification of 17 flaviviruses using the neighbor-joining tree method. The analyses of the sequence data produced dendrograms which supported the traditional groupings based on serological evidence, and they suggested that the flaviviruses have evolved by divergent mutational change and there was no evidence of genetic recombination between members of the group. Comparisons of the sequences of the flavivirus polymerase and helicase-like proteins (NS5 and NS3, respectively) with those from other viruses yielded a classification of the flaviviruses indicating that the primary division of the flaviviruses was between those transmitted by mosquitoes and those transmitted by ticks.</p></abstract></profileDesc><revisionDesc><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RJQPJ5N5-N/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>92904607</aid><ce:pii>0042-6822(92)90460-7</ce:pii><ce:doi>10.1016/0042-6822(92)90460-7</ce:doi><ce:copyright type="unknown" year="1992"></ce:copyright></item-info><head><ce:article-footnote><ce:label>☆</ce:label><ce:note-para>Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. M85258 and M85259</ce:note-para></ce:article-footnote><ce:title>Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses</ce:title><ce:author-group><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Blok</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="COR1"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>S.M.</ce:given-name><ce:surname>McWilliam</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>H.C.</ce:given-name><ce:surname>Butler</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.J.</ce:given-name><ce:surname>Gibbs</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>G.</ce:given-name><ce:surname>Weiller</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>B.L.</ce:given-name><ce:surname>Herring</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.C.</ce:given-name><ce:surname>Hemsley</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.G.</ce:given-name><ce:surname>Aaskov</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>S.</ce:given-name><ce:surname>Yoksan</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>‡</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>N.</ce:given-name><ce:surname>Bhamarapravati</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>‡</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>∗</ce:label><ce:textfn>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>†</ce:label><ce:textfn>Queensland University of Technology, George St., Brisbane, Queensland 4000, Australia</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>‡</ce:label><ce:textfn>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>2</ce:label><ce:text>To whom correspondence and reprint requests should be addressed.</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>3</ce:label><ce:note-para>Present address: CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Brisbane, Queensland, 4068, Australia.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="10" month="9" year="1991"></ce:date-received><ce:date-accepted day="2" month="12" year="1991"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A comparison of the sequence of the dengue-2 16681 virus with that of the candidate vaccine strain (16681-PDK53) derived from it identified 53 of the 10,723 nucleotides which differed between the strains. Nucleotide changes occurred in genes coding for all virion and nonvirion proteins, and in the 5′ and 3′ untranslated regions. Twenty-seven of the nucleotide changes resulted in amino acid alterations. The greatest amino acid sequence differences in the virion proteins occurred in prM (2.20%; <math altimg="si1.gif"><fr shape="sol"><nu>2</nu><de>91</de></fr></math> amino acids) followed by the M protein (1.33%; <math altimg="si2.gif"><fr shape="sol"><nu>1</nu><de>75</de></fr></math> amino acids), the C protein (0.88%; <math altimg="si3.gif"><fr shape="sol"><nu>1</nu><de>114</de></fr></math> amino acid), and the E protein (0.61%; <math altimg="si4.gif"><fr shape="sol"><nu>3</nu><de>495</de></fr></math> amino acids). Differences in the amino acid sequence of nonvirion proteins ranged from 1.51% (<math altimg="si5.gif"><fr shape="sol"><nu>6</nu><de>398</de></fr></math> amino acids) in NS4 to 0.33% (<math altimg="si6.gif"><fr shape="sol"><nu>3</nu><de>900</de></fr></math> amino acids) in NS5. The encoded protein sequences of 16681-PDK53 were also compared with the published sequences of other flaviviruses to obtain a detailed classification of 17 flaviviruses using the neighbor-joining tree method. The analyses of the sequence data produced dendrograms which supported the traditional groupings based on serological evidence, and they suggested that the flaviviruses have evolved by divergent mutational change and there was no evidence of genetic recombination between members of the group. Comparisons of the sequences of the flavivirus polymerase and helicase-like proteins (NS5 and NS3, respectively) with those from other viruses yielded a classification of the flaviviruses indicating that the primary division of the flaviviruses was between those transmitted by mosquitoes and those transmitted by ticks.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Comparison of a dengue-2 virus and its candidate vaccine derivative: Sequence relationships with the flaviviruses and other viruses</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Blok</namePart><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation><affiliation>To whom correspondence and reprint requests should be addressed.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.M.</namePart><namePart type="family">McWilliam</namePart><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation><affiliation>3Present address: CSIRO Division of Tropical Animal Production, Long Pocket Laboratories, Brisbane, Queensland, 4068, Australia.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.C.</namePart><namePart type="family">Butler</namePart><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.J.</namePart><namePart type="family">Gibbs</namePart><affiliation>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Weiller</namePart><affiliation>Molecular Evolution and Systematics Group, Research School of Biological Sciences, Australian National University, Canberra City, ACT 2601, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.L.</namePart><namePart type="family">Herring</namePart><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.C.</namePart><namePart type="family">Hemsley</namePart><affiliation>Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Herston Rd, Brisbane, Queensland 4029, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.G.</namePart><namePart type="family">Aaskov</namePart><affiliation>Queensland University of Technology, George St., Brisbane, Queensland 4000, Australia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Yoksan</namePart><affiliation>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Bhamarapravati</namePart><affiliation>Center for Vaccine Development, Mahidol University, Nakhon Pathom, Thailand</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A comparison of the sequence of the dengue-2 16681 virus with that of the candidate vaccine strain (16681-PDK53) derived from it identified 53 of the 10,723 nucleotides which differed between the strains. Nucleotide changes occurred in genes coding for all virion and nonvirion proteins, and in the 5′ and 3′ untranslated regions. Twenty-seven of the nucleotide changes resulted in amino acid alterations. The greatest amino acid sequence differences in the virion proteins occurred in prM (2.20%; 291 amino acids) followed by the M protein (1.33%; 175 amino acids), the C protein (0.88%; 1114 amino acid), and the E protein (0.61%; 3495 amino acids). Differences in the amino acid sequence of nonvirion proteins ranged from 1.51% (6398 amino acids) in NS4 to 0.33% (3900 amino acids) in NS5. The encoded protein sequences of 16681-PDK53 were also compared with the published sequences of other flaviviruses to obtain a detailed classification of 17 flaviviruses using the neighbor-joining tree method. The analyses of the sequence data produced dendrograms which supported the traditional groupings based on serological evidence, and they suggested that the flaviviruses have evolved by divergent mutational change and there was no evidence of genetic recombination between members of the group. Comparisons of the sequences of the flavivirus polymerase and helicase-like proteins (NS5 and NS3, respectively) with those from other viruses yielded a classification of the flaviviruses indicating that the primary division of the flaviviruses was between those transmitted by mosquitoes and those transmitted by ticks.</abstract><note>Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession Nos. M85258 and M85259</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0441-2</identifier><part><date>1992</date><detail type="volume"><number>187</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>391</start><end>858</end></extent><extent unit="pages"><start>573</start><end>590</end></extent></part></relatedItem><identifier type="istex">4D6F87B101DF40A6F8C49821E40BEE30F0577DE3</identifier><identifier type="ark">ark:/67375/6H6-RJQPJ5N5-N</identifier><identifier type="DOI">10.1016/0042-6822(92)90460-7</identifier><identifier type="PII">0042-6822(92)90460-7</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-RJQPJ5N5-N/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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