Messenger rna for the ad2 dna binding protein: dna sequences encoding the first leader and heterogeneity at the mRNA 5′ end
Identifieur interne : 002536 ( Istex/Corpus ); précédent : 002535; suivant : 002537Messenger rna for the ad2 dna binding protein: dna sequences encoding the first leader and heterogeneity at the mRNA 5′ end
Auteurs : C. C. Baker ; J. Herisse ; G. Courtois ; F. Galibert ; E. ZiffSource :
- Cell [ 0092-8674 ] ; 1979.
English descriptors
- Teeft :
- Acad, Adenovirus, Adenovirus type, Berk, Binding protein, Biol, Darnell, Deae paper, Digestion, Early region, Early regions, Eucaryotic, First leader, Fraser, Galibert, Hela, Hela cells, Heterogeneity, Homology, Hybrid, Hybridized, Initiation site, Late mrna, Mrna, Nuclease, Nucleotide, Nucleotide sequence, Nucleotide sequences, Oligonucleotide, Oligonucleotides, Personal communication, Polymerase, Polyoma, Proc, Promoter, Protein mrna, Residue, Rnaase, Rnaase components, Second leader, Simian virus, Splice, Splice point, Tataaa, Terminus, Transcription, Transcription unit, Transcription units, Unpublished observations, Viral, Ziff.
Abstract
Abstract: During the early stage of Ad2 infection of human cells, RNA is transcribed from five separate transcription units. Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the flanking sequences in the DNA. The leader, labeled in vivo with 32P, was isolated by DNA filter hybridization to the viral restriction fragment Eco RI F, and its RNAase T1 and RNAase A oligonucleotides were analyzed by RNA fingerprinting techniques. Comparison of this RNA sequence information with the DNA sequence of Eco RI F has located a 68 nucleotide region of the Has III C subfragment at coordinate 75.1 that encodes the leader. This position is near the coordinate to which nascent chain analysis and ultraviolet transcription mapping have mapped an RNA initiation site, or promoter, for the DBP mRNA. The DNA sequence that overlaps the leader on the 3′ side contains a donor sequence for splicing this leader to a second downstream leader. The splicing sequence shows a seven base homology with the comparable structure of the Ad2 major late leader, and a mouse globin mRNA splicing sequence. The DNA sequence upstream from the cap, the region of the potential promoter site does not, however, contain a “TA-TAAA”-type homology of the sort noted by D. Hogness, M. Goldberg and R. Lifton (personal communication) for many cellular transcription units, and by other investigators for the Ad2 major late transcription unit. Also, the leader is found with two distinct capped 5′ termini, 7meGpppA and 7meGpppG, which are encoded at adjacent positions in the DNA and thus are from mRNAs which are staggered by one nucleotide in length at the 5′ end. The staggering at the 5′ terminus and the lack of the upstream homology distinguish the DBP mRNA from many viral and cellular messengers. In both these respects, however, the DBP mRNA resembles the late messengers of SV40 and polyoma viruses. In this paper, we discuss the implications of these findings for the mechanism of specifying mRNA 5′ ends.
Url:
DOI: 10.1016/0092-8674(79)90073-4
Links to Exploration step
ISTEX:321F7FF59AA569B6FA460CAEF9CE83F21725B491Le document en format XML
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Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the flanking sequences in the DNA. The leader, labeled in vivo with 32P, was isolated by DNA filter hybridization to the viral restriction fragment Eco RI F, and its RNAase T1 and RNAase A oligonucleotides were analyzed by RNA fingerprinting techniques. Comparison of this RNA sequence information with the DNA sequence of Eco RI F has located a 68 nucleotide region of the Has III C subfragment at coordinate 75.1 that encodes the leader. This position is near the coordinate to which nascent chain analysis and ultraviolet transcription mapping have mapped an RNA initiation site, or promoter, for the DBP mRNA. The DNA sequence that overlaps the leader on the 3′ side contains a donor sequence for splicing this leader to a second downstream leader. The splicing sequence shows a seven base homology with the comparable structure of the Ad2 major late leader, and a mouse globin mRNA splicing sequence. The DNA sequence upstream from the cap, the region of the potential promoter site does not, however, contain a “TA-TAAA”-type homology of the sort noted by D. Hogness, M. Goldberg and R. Lifton (personal communication) for many cellular transcription units, and by other investigators for the Ad2 major late transcription unit. Also, the leader is found with two distinct capped 5′ termini, 7meGpppA and 7meGpppG, which are encoded at adjacent positions in the DNA and thus are from mRNAs which are staggered by one nucleotide in length at the 5′ end. The staggering at the 5′ terminus and the lack of the upstream homology distinguish the DBP mRNA from many viral and cellular messengers. In both these respects, however, the DBP mRNA resembles the late messengers of SV40 and polyoma viruses. In this paper, we discuss the implications of these findings for the mechanism of specifying mRNA 5′ ends.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>rnaase</json:string><json:string>mrna</json:string><json:string>oligonucleotides</json:string><json:string>adenovirus</json:string><json:string>homology</json:string><json:string>ziff</json:string><json:string>nuclease</json:string><json:string>biol</json:string><json:string>heterogeneity</json:string><json:string>polymerase</json:string><json:string>oligonucleotide</json:string><json:string>terminus</json:string><json:string>berk</json:string><json:string>first leader</json:string><json:string>viral</json:string><json:string>polyoma</json:string><json:string>tataaa</json:string><json:string>transcription</json:string><json:string>darnell</json:string><json:string>fraser</json:string><json:string>acad</json:string><json:string>hybridized</json:string><json:string>proc</json:string><json:string>promoter</json:string><json:string>initiation site</json:string><json:string>hela</json:string><json:string>eucaryotic</json:string><json:string>binding protein</json:string><json:string>digestion</json:string><json:string>transcription unit</json:string><json:string>galibert</json:string><json:string>nucleotide</json:string><json:string>splice point</json:string><json:string>hybrid</json:string><json:string>hela cells</json:string><json:string>late mrna</json:string><json:string>personal communication</json:string><json:string>splice</json:string><json:string>early region</json:string><json:string>early regions</json:string><json:string>transcription units</json:string><json:string>adenovirus type</json:string><json:string>unpublished observations</json:string><json:string>nucleotide sequences</json:string><json:string>rnaase components</json:string><json:string>second leader</json:string><json:string>deae paper</json:string><json:string>nucleotide sequence</json:string><json:string>protein mrna</json:string><json:string>simian virus</json:string><json:string>residue</json:string></teeft></keywords><author><json:item><name>C.C. 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Ziff</name><affiliations><json:string>The Rockefeller University New York, New York 10021 USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-SMS2W22T-4</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: During the early stage of Ad2 infection of human cells, RNA is transcribed from five separate transcription units. Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the flanking sequences in the DNA. The leader, labeled in vivo with 32P, was isolated by DNA filter hybridization to the viral restriction fragment Eco RI F, and its RNAase T1 and RNAase A oligonucleotides were analyzed by RNA fingerprinting techniques. Comparison of this RNA sequence information with the DNA sequence of Eco RI F has located a 68 nucleotide region of the Has III C subfragment at coordinate 75.1 that encodes the leader. This position is near the coordinate to which nascent chain analysis and ultraviolet transcription mapping have mapped an RNA initiation site, or promoter, for the DBP mRNA. The DNA sequence that overlaps the leader on the 3′ side contains a donor sequence for splicing this leader to a second downstream leader. The splicing sequence shows a seven base homology with the comparable structure of the Ad2 major late leader, and a mouse globin mRNA splicing sequence. The DNA sequence upstream from the cap, the region of the potential promoter site does not, however, contain a “TA-TAAA”-type homology of the sort noted by D. Hogness, M. Goldberg and R. Lifton (personal communication) for many cellular transcription units, and by other investigators for the Ad2 major late transcription unit. Also, the leader is found with two distinct capped 5′ termini, 7meGpppA and 7meGpppG, which are encoded at adjacent positions in the DNA and thus are from mRNAs which are staggered by one nucleotide in length at the 5′ end. The staggering at the 5′ terminus and the lack of the upstream homology distinguish the DBP mRNA from many viral and cellular messengers. In both these respects, however, the DBP mRNA resembles the late messengers of SV40 and polyoma viruses. In this paper, we discuss the implications of these findings for the mechanism of specifying mRNA 5′ ends.</abstract><qualityIndicators><score>10</score><pdfWordCount>6855</pdfWordCount><pdfCharCount>44897</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>625.4 x 827.84 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>351</abstractWordCount><abstractCharCount>2129</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Messenger rna for the ad2 dna binding protein: dna sequences encoding the first leader and heterogeneity at the mRNA 5′ end</title><pmid><json:string>227610</json:string></pmid><pii><json:string>0092-8674(79)90073-4</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Nat. Acad. Sci. 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Maniatis</json:string><json:string>A. Efstratiadis</json:string><json:string>Ad</json:string><json:string>T. Arrand</json:string><json:string>J. Arrand</json:string><json:string>Paris Cedex</json:string><json:string>R. Lifton</json:string><json:string>J. Nevins</json:string><json:string>Heterogeneity</json:string><json:string>E. Ziff</json:string><json:string>F. Galibertt</json:string><json:string>H. Ginsberg</json:string><json:string>In</json:string><json:string>U.S.C. Section</json:string><json:string>D. Hogness</json:string><json:string>J. Blanchard</json:string><json:string>C. C. Baker</json:string><json:string>A. Cowie</json:string><json:string>M. Wilson</json:string><json:string>A. Flavell</json:string><json:string>O.Ol</json:string><json:string>J. Herisse</json:string><json:string>G. Courtois</json:string><json:string>J. Darnell</json:string><json:string>E. Lacey</json:string></persName><placeName><json:string>Kamen</json:string><json:string>York</json:string><json:string>Goldberg</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Ziff and Evans (1978)</json:string><json:string>Berk and Sharp, 1976</json:string><json:string>Price and Penman, 1972</json:string><json:string>Lockard and RajBhandary, 1976</json:string><json:string>Haegeman and Fiers, 1978</json:string><json:string>Carter and Blanton, 1978a, 1978b</json:string><json:string>Maxam and Gilbert (1977)</json:string><json:string>Chow et al., 1977a</json:string><json:string>Weber, Jelinek and Darnell, 1977</json:string><json:string>Ginsberg et al., 1974</json:string><json:string>Lewis et al., 1976</json:string><json:string>Buttner, Veres-Molnar and Green, 1976</json:string><json:string>Galibert et al.</json:string><json:string>Kitchingman, Lai and Westfall, 1977</json:string><json:string>Breathnach et al., 1978</json:string><json:string>Pribnow, 1979</json:string><json:string>Fraser and Ziff (1978)</json:string><json:string>Hagenbuchle et al., 1978</json:string><json:string>Reddy et al., 1978</json:string><json:string>Fiers et al., 1978</json:string><json:string>Chow, Broker and Lewis, 1979</json:string><json:string>Blanton and Carter, 1979</json:string><json:string>Shatkin, 1976</json:string><json:string>van der Vliet and Sussenbach, 1975</json:string><json:string>Sedat and Ziff (1974)</json:string><json:string>Frenkel and Ensinger, 1974</json:string><json:string>Nevins and Darnell, 1978</json:string><json:string>estimated to be approximately 50 residues long; Berk and Sharp, 1978</json:string><json:string>van der Vliet and Levine, 1973</json:string><json:string>Kitchingman et al., 1977</json:string><json:string>Flavell et al., 1979</json:string><json:string>for review see Flint, 1977</json:string><json:string>Maxim and Gilbert (1977)</json:string><json:string>Berk and Sharp, 1978</json:string><json:string>see Gilbert, 1976</json:string><json:string>van der Vliet et al., 1975</json:string><json:string>Akusjarvi and Pettersson, 1979</json:string><json:string>Cheng and Kazazian, 1977</json:string><json:string>Sehgal, Fraser and Darnell, 1979</json:string><json:string>Galibert, Herisse and Courtois, 1979</json:string><json:string>Evans et al., 1977</json:string><json:string>Wallace and Kates, 1972</json:string><json:string>Tonegawa et al., 1978</json:string><json:string>Zain et al., 1979</json:string><json:string>Adams and Gory, 1975</json:string><json:string>Berk and Sharp, 1977</json:string><json:string>Cory and Adams, 1975</json:string><json:string>Edmonds et al., 1976</json:string><json:string>Goldberg, Weber and Darnell, 1977</json:string><json:string>Konkel, Tilghman and Leder, 1978</json:string><json:string>Tsujimoto and Suzuki, 1979</json:string><json:string>Furuichi et al., 1975</json:string><json:string>Wilson, Fraser and Darnell, 1979</json:string><json:string>Konkel et al., 1978</json:string><json:string>Chow et al., 1977b</json:string><json:string>Ghosh et al., 1978, 1979</json:string><json:string>for review see Darnell, 1979</json:string><json:string>Ghosh et al., 1979</json:string><json:string>Ziff and Evans, 1978</json:string><json:string>Kitchingman et al.</json:string><json:string>Caterall et al., 1978</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-SMS2W22T-4</json:string></ark><categories><wos><json:string>1 - 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Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the flanking sequences in the DNA. The leader, labeled in vivo with 32P, was isolated by DNA filter hybridization to the viral restriction fragment Eco RI F, and its RNAase T1 and RNAase A oligonucleotides were analyzed by RNA fingerprinting techniques. Comparison of this RNA sequence information with the DNA sequence of Eco RI F has located a 68 nucleotide region of the Has III C subfragment at coordinate 75.1 that encodes the leader. This position is near the coordinate to which nascent chain analysis and ultraviolet transcription mapping have mapped an RNA initiation site, or promoter, for the DBP mRNA. The DNA sequence that overlaps the leader on the 3′ side contains a donor sequence for splicing this leader to a second downstream leader. The splicing sequence shows a seven base homology with the comparable structure of the Ad2 major late leader, and a mouse globin mRNA splicing sequence. The DNA sequence upstream from the cap, the region of the potential promoter site does not, however, contain a “TA-TAAA”-type homology of the sort noted by D. Hogness, M. Goldberg and R. Lifton (personal communication) for many cellular transcription units, and by other investigators for the Ad2 major late transcription unit. Also, the leader is found with two distinct capped 5′ termini, 7meGpppA and 7meGpppG, which are encoded at adjacent positions in the DNA and thus are from mRNAs which are staggered by one nucleotide in length at the 5′ end. The staggering at the 5′ terminus and the lack of the upstream homology distinguish the DBP mRNA from many viral and cellular messengers. In both these respects, however, the DBP mRNA resembles the late messengers of SV40 and polyoma viruses. In this paper, we discuss the implications of these findings for the mechanism of specifying mRNA 5′ ends.</p></abstract></profileDesc><revisionDesc><change when="1979">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-SMS2W22T-4/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CELL</jid><aid>79900734</aid><ce:pii>0092-8674(79)90073-4</ce:pii><ce:doi>10.1016/0092-8674(79)90073-4</ce:doi><ce:copyright type="unknown" year="1979"></ce:copyright></item-info><head><ce:title>Messenger rna for the ad2 dna binding protein: dna sequences encoding the first leader and heterogeneity at the mRNA 5′ end</ce:title><ce:author-group><ce:author><ce:given-name>C.C.</ce:given-name><ce:surname>Baker</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Herisse</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>G.</ce:given-name><ce:surname>Courtois</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>F.</ce:given-name><ce:surname>Galibert</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Ziff</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>The Rockefeller University New York, New York 10021 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Laboratoire d'Hematologie Experimentale Centre Hayem Hopital Saint-Louis 75475 Paris Cedex 10, France</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="13" month="7" year="1979"></ce:date-received><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>During the early stage of Ad2 infection of human cells, RNA is transcribed from five separate transcription units. Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the flanking sequences in the DNA. The leader, labeled in vivo with <ce:sup>32</ce:sup>P, was isolated by DNA filter hybridization to the viral restriction fragment Eco RI F, and its RNAase T1 and RNAase A oligonucleotides were analyzed by RNA fingerprinting techniques. Comparison of this RNA sequence information with the DNA sequence of Eco RI F has located a 68 nucleotide region of the Has III C subfragment at coordinate 75.1 that encodes the leader. This position is near the coordinate to which nascent chain analysis and ultraviolet transcription mapping have mapped an RNA initiation site, or promoter, for the DBP mRNA. The DNA sequence that overlaps the leader on the 3′ side contains a donor sequence for splicing this leader to a second downstream leader. The splicing sequence shows a seven base homology with the comparable structure of the Ad2 major late leader, and a mouse globin mRNA splicing sequence. The DNA sequence upstream from the cap, the region of the potential promoter site does not, however, contain a “TA-TAAA”-type homology of the sort noted by D. Hogness, M. Goldberg and R. Lifton (personal communication) for many cellular transcription units, and by other investigators for the Ad2 major late transcription unit. Also, the leader is found with two distinct capped 5′ termini, <ce:sup loc="pre">7me</ce:sup>GpppA and <ce:sup loc="pre">7me</ce:sup>GpppG, which are encoded at adjacent positions in the DNA and thus are from mRNAs which are staggered by one nucleotide in length at the 5′ end. The staggering at the 5′ terminus and the lack of the upstream homology distinguish the DBP mRNA from many viral and cellular messengers. In both these respects, however, the DBP mRNA resembles the late messengers of SV40 and polyoma viruses. In this paper, we discuss the implications of these findings for the mechanism of specifying mRNA 5′ ends.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Messenger rna for the ad2 dna binding protein: dna sequences encoding the first leader and heterogeneity at the mRNA 5′ end</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Messenger rna for the ad2 dna binding protein: dna sequences encoding the first leader and heterogeneity at the mRNA 5′ end</title></titleInfo><name type="personal"><namePart type="given">C.C.</namePart><namePart type="family">Baker</namePart><affiliation>The Rockefeller University New York, New York 10021 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Herisse</namePart><affiliation>Laboratoire d'Hematologie Experimentale Centre Hayem Hopital Saint-Louis 75475 Paris Cedex 10, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Courtois</namePart><affiliation>Laboratoire d'Hematologie Experimentale Centre Hayem Hopital Saint-Louis 75475 Paris Cedex 10, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Galibert</namePart><affiliation>Laboratoire d'Hematologie Experimentale Centre Hayem Hopital Saint-Louis 75475 Paris Cedex 10, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Ziff</namePart><affiliation>The Rockefeller University New York, New York 10021 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1979</dateIssued><copyrightDate encoding="w3cdtf">1979</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: During the early stage of Ad2 infection of human cells, RNA is transcribed from five separate transcription units. Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the flanking sequences in the DNA. The leader, labeled in vivo with 32P, was isolated by DNA filter hybridization to the viral restriction fragment Eco RI F, and its RNAase T1 and RNAase A oligonucleotides were analyzed by RNA fingerprinting techniques. Comparison of this RNA sequence information with the DNA sequence of Eco RI F has located a 68 nucleotide region of the Has III C subfragment at coordinate 75.1 that encodes the leader. This position is near the coordinate to which nascent chain analysis and ultraviolet transcription mapping have mapped an RNA initiation site, or promoter, for the DBP mRNA. The DNA sequence that overlaps the leader on the 3′ side contains a donor sequence for splicing this leader to a second downstream leader. The splicing sequence shows a seven base homology with the comparable structure of the Ad2 major late leader, and a mouse globin mRNA splicing sequence. The DNA sequence upstream from the cap, the region of the potential promoter site does not, however, contain a “TA-TAAA”-type homology of the sort noted by D. Hogness, M. Goldberg and R. Lifton (personal communication) for many cellular transcription units, and by other investigators for the Ad2 major late transcription unit. Also, the leader is found with two distinct capped 5′ termini, 7meGpppA and 7meGpppG, which are encoded at adjacent positions in the DNA and thus are from mRNAs which are staggered by one nucleotide in length at the 5′ end. The staggering at the 5′ terminus and the lack of the upstream homology distinguish the DBP mRNA from many viral and cellular messengers. In both these respects, however, the DBP mRNA resembles the late messengers of SV40 and polyoma viruses. In this paper, we discuss the implications of these findings for the mechanism of specifying mRNA 5′ ends.</abstract><relatedItem type="host"><titleInfo><title>Cell</title></titleInfo><titleInfo type="abbreviated"><title>CELL</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1979</dateIssued></originInfo><identifier type="ISSN">0092-8674</identifier><identifier type="PII">S0092-8674(00)X0495-3</identifier><part><date>1979</date><detail type="volume"><number>18</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>235</start><end>611</end></extent><extent unit="pages"><start>569</start><end>580</end></extent></part></relatedItem><identifier type="istex">321F7FF59AA569B6FA460CAEF9CE83F21725B491</identifier><identifier type="ark">ark:/67375/6H6-SMS2W22T-4</identifier><identifier type="DOI">10.1016/0092-8674(79)90073-4</identifier><identifier type="PII">0092-8674(79)90073-4</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-SMS2W22T-4/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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