A multiplicity of CCAAT box-binding proteins
Identifieur interne : 002511 ( Istex/Corpus ); précédent : 002510; suivant : 002512A multiplicity of CCAAT box-binding proteins
Auteurs : Arnulf Dorn ; Jacques Bollekens ; Adrian Staub ; Christophe Benoist ; Diane MathisSource :
- Cell [ 0092-8674 ] ; 1987.
English descriptors
- Teeft :
- Antisense, Antisense strand, Antisense strands, Arrows point, Assay, Band intensity, Binding, Binding core, Binding efficiency, Binding properties, Binding proteins, Binding reaction, Binding requirements, Binding site, Biol, Ccaat, Ccaat boxes, Ccaat protein, Ccaat proteins, Ccaat sequence, Ccaat sequences, Chicken liver, Cleavage, Cleavage products, Competition experiments, Complex formation, Contact sites, Different entities, Distinct proteins, Dna, Dnaase, Dorn, Element harbors, Ethanol precipitation, Experimental evidence, Fetal haemoglobin, Fragment, Functional relevance, Gene, Gene expression, Gene promoters, Good correlation, Heat shock transcription factor, Hela, Hela cell, Hela cells, Kelly, Major histocompatibility, Mcknight, Methylated, Methylation, Methylation interference, Methylation interference mapping, Mouse promoter, Murine, Murine class, Mutagenesis, Mutation, Neutral polyacrylamide, Nuclear protein, Nuclear proteins, Nucleotide, Oligo, Oligonucleotide, Oligonucleotides, Oligos, Other hand, Other oligonucleotides, Point mutation, Polyacrylamide, Promoter, Promoter fragment, Promoter function, Promoter region, Purine contacts, Random sequence, Relative binding efficiencies, Retardation, Retardation assay, Retardation assays, Retarded band, Room temperature, Same protein, Saturation mutagenesis, Saturation mutagenesis experiment, Sense strand, Sequence elements, Sequencing, Specific binding, Thymidine kinase promoter, Tjian, Transcription, Transcription factor, Transcriptional, Transcriptional control elements, Typical binding reaction, Unlabeled, Unlabeled competitor oligonucleotide.
Abstract
Abstract: NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.
Url:
DOI: 10.1016/0092-8674(87)90513-7
Links to Exploration step
ISTEX:523F85199F114B4B17AEEAF87EFEBEFCCE27C897Le document en format XML
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Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Benoist, Christophe" sort="Benoist, Christophe" uniqKey="Benoist C" first="Christophe" last="Benoist">Christophe Benoist</name><affiliation><mods:affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Mathis, Diane" sort="Mathis, Diane" uniqKey="Mathis D" first="Diane" last="Mathis">Diane Mathis</name><affiliation><mods:affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987">1987</date><biblScope unit="volume">50</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="863">863</biblScope><biblScope unit="page" to="872">872</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antisense</term><term>Antisense strand</term><term>Antisense strands</term><term>Arrows point</term><term>Assay</term><term>Band intensity</term><term>Binding</term><term>Binding core</term><term>Binding efficiency</term><term>Binding properties</term><term>Binding proteins</term><term>Binding reaction</term><term>Binding requirements</term><term>Binding site</term><term>Biol</term><term>Ccaat</term><term>Ccaat boxes</term><term>Ccaat protein</term><term>Ccaat proteins</term><term>Ccaat sequence</term><term>Ccaat sequences</term><term>Chicken liver</term><term>Cleavage</term><term>Cleavage products</term><term>Competition experiments</term><term>Complex formation</term><term>Contact sites</term><term>Different entities</term><term>Distinct proteins</term><term>Dna</term><term>Dnaase</term><term>Dorn</term><term>Element harbors</term><term>Ethanol precipitation</term><term>Experimental evidence</term><term>Fetal haemoglobin</term><term>Fragment</term><term>Functional relevance</term><term>Gene</term><term>Gene expression</term><term>Gene promoters</term><term>Good correlation</term><term>Heat shock transcription factor</term><term>Hela</term><term>Hela cell</term><term>Hela cells</term><term>Kelly</term><term>Major histocompatibility</term><term>Mcknight</term><term>Methylated</term><term>Methylation</term><term>Methylation interference</term><term>Methylation interference mapping</term><term>Mouse promoter</term><term>Murine</term><term>Murine class</term><term>Mutagenesis</term><term>Mutation</term><term>Neutral polyacrylamide</term><term>Nuclear protein</term><term>Nuclear proteins</term><term>Nucleotide</term><term>Oligo</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Oligos</term><term>Other hand</term><term>Other oligonucleotides</term><term>Point mutation</term><term>Polyacrylamide</term><term>Promoter</term><term>Promoter fragment</term><term>Promoter function</term><term>Promoter region</term><term>Purine contacts</term><term>Random sequence</term><term>Relative binding efficiencies</term><term>Retardation</term><term>Retardation assay</term><term>Retardation assays</term><term>Retarded band</term><term>Room temperature</term><term>Same protein</term><term>Saturation mutagenesis</term><term>Saturation mutagenesis experiment</term><term>Sense strand</term><term>Sequence elements</term><term>Sequencing</term><term>Specific binding</term><term>Thymidine kinase promoter</term><term>Tjian</term><term>Transcription</term><term>Transcription factor</term><term>Transcriptional</term><term>Transcriptional control elements</term><term>Typical binding reaction</term><term>Unlabeled</term><term>Unlabeled competitor oligonucleotide</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>ccaat</json:string><json:string>oligonucleotide</json:string><json:string>oligonucleotides</json:string><json:string>promoter</json:string><json:string>oligo</json:string><json:string>mutation</json:string><json:string>mcknight</json:string><json:string>retardation assay</json:string><json:string>methylation</json:string><json:string>assay</json:string><json:string>dna</json:string><json:string>dnaase</json:string><json:string>ccaat sequence</json:string><json:string>polyacrylamide</json:string><json:string>murine</json:string><json:string>tjian</json:string><json:string>mutagenesis</json:string><json:string>biol</json:string><json:string>dorn</json:string><json:string>transcriptional</json:string><json:string>sequencing</json:string><json:string>ccaat boxes</json:string><json:string>ccaat protein</json:string><json:string>methylated</json:string><json:string>binding site</json:string><json:string>hela</json:string><json:string>antisense</json:string><json:string>unlabeled</json:string><json:string>oligos</json:string><json:string>transcription</json:string><json:string>ccaat sequences</json:string><json:string>retarded band</json:string><json:string>nucleotide</json:string><json:string>retardation assays</json:string><json:string>promoter fragment</json:string><json:string>distinct proteins</json:string><json:string>retardation</json:string><json:string>kelly</json:string><json:string>room temperature</json:string><json:string>chicken liver</json:string><json:string>saturation mutagenesis experiment</json:string><json:string>binding core</json:string><json:string>ccaat proteins</json:string><json:string>hela cell</json:string><json:string>random sequence</json:string><json:string>major histocompatibility</json:string><json:string>antisense strand</json:string><json:string>competition experiments</json:string><json:string>murine class</json:string><json:string>transcription factor</json:string><json:string>sequence elements</json:string><json:string>binding efficiency</json:string><json:string>methylation interference mapping</json:string><json:string>gene</json:string><json:string>unlabeled competitor oligonucleotide</json:string><json:string>gene expression</json:string><json:string>fetal haemoglobin</json:string><json:string>cleavage products</json:string><json:string>element harbors</json:string><json:string>antisense strands</json:string><json:string>binding reaction</json:string><json:string>arrows point</json:string><json:string>typical binding reaction</json:string><json:string>saturation mutagenesis</json:string><json:string>sense strand</json:string><json:string>experimental evidence</json:string><json:string>promoter function</json:string><json:string>promoter region</json:string><json:string>other oligonucleotides</json:string><json:string>transcriptional control elements</json:string><json:string>specific binding</json:string><json:string>complex formation</json:string><json:string>same protein</json:string><json:string>good correlation</json:string><json:string>relative binding efficiencies</json:string><json:string>contact sites</json:string><json:string>binding properties</json:string><json:string>nuclear protein</json:string><json:string>binding proteins</json:string><json:string>neutral polyacrylamide</json:string><json:string>other hand</json:string><json:string>gene promoters</json:string><json:string>methylation interference</json:string><json:string>thymidine kinase promoter</json:string><json:string>heat shock transcription factor</json:string><json:string>different entities</json:string><json:string>purine contacts</json:string><json:string>binding requirements</json:string><json:string>nuclear proteins</json:string><json:string>functional relevance</json:string><json:string>point mutation</json:string><json:string>mouse promoter</json:string><json:string>ethanol precipitation</json:string><json:string>hela cells</json:string><json:string>band intensity</json:string><json:string>binding</json:string><json:string>cleavage</json:string><json:string>fragment</json:string></teeft></keywords><author><json:item><name>Arnulf Dorn</name><affiliations><json:string>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</json:string></affiliations></json:item><json:item><name>Jacques Bollekens</name><affiliations><json:string>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</json:string></affiliations></json:item><json:item><name>Adrian Staub</name><affiliations><json:string>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</json:string></affiliations></json:item><json:item><name>Christophe Benoist</name><affiliations><json:string>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</json:string></affiliations></json:item><json:item><name>Diane Mathis</name><affiliations><json:string>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-J69FD1W8-R</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.</abstract><qualityIndicators><score>8.5</score><pdfWordCount>6954</pdfWordCount><pdfCharCount>46118</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>612.28 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>125</abstractWordCount><abstractCharCount>900</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>A multiplicity of CCAAT box-binding proteins</title><pmid><json:string>3476205</json:string></pmid><pii><json:string>0092-8674(87)90513-7</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. USA</title><language><json:string>unknown</json:string></language><volume>82</volume><pages><first>5676</first><last>5680</last></pages></serie><host><title>Cell</title><language><json:string>unknown</json:string></language><publicationDate>1987</publicationDate><issn><json:string>0092-8674</json:string></issn><pii><json:string>S0092-8674(00)X0268-1</json:string></pii><volume>50</volume><issue>6</issue><pages><first>863</first><last>872</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1987</json:string></date><geogName></geogName><orgName><json:string>Johns Hopkins University</json:string><json:string>Carnegie Institute, Baltimore, MD, USA</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Diane Mathis</json:string><json:string>C. Werle</json:string><json:string>C.N.R.S. Unit</json:string><json:string>B. Eoulay</json:string><json:string>S. McKnight</json:string><json:string>R. Rupp</json:string><json:string>Christophe Benoist</json:string><json:string>W.T. Mutant</json:string><json:string>A. Promoter</json:string><json:string>B. Figure</json:string><json:string>A. Dorn</json:string><json:string>U.S.C. Section</json:string><json:string>E. O’Neill</json:string><json:string>D. Mathis</json:string><json:string>Adrian Staub</json:string><json:string>A. Ney</json:string><json:string>A. Sippel</json:string><json:string>C. Benoist</json:string><json:string>I. Data</json:string></persName><placeName><json:string>Heidelberg</json:string><json:string>USA</json:string><json:string>Strasbourg</json:string><json:string>Baltimore</json:string><json:string>MD</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Bienz and Pelham, 1988</json:string><json:string>Graves et al., 1985a</json:string><json:string>Busslinger and Barberis, 1966</json:string><json:string>Coen et al., 1986</json:string><json:string>Dorn et al., 1987</json:string><json:string>Leegwater et al., 1986</json:string><json:string>Mathis et al., 1983</json:string><json:string>McKnight and Tjian, 1986</json:string><json:string>Morgan et al., 1987</json:string><json:string>Dierks et al., 1983</json:string><json:string>Sullivan et al., 1986</json:string><json:string>Efstratiadis et al., 1960</json:string><json:string>Gronostajski, 1986</json:string><json:string>Collins et al., 1985</json:string><json:string>McKnight and Tjian, 1968</json:string><json:string>Calladine, 1982</json:string><json:string>Saito et al., 1983</json:string><json:string>Gelinas et al., 1985</json:string><json:string>Cohen et al., 1966</json:string><json:string>Auffray and Strominger, 1986</json:string><json:string>Waksman, 1985</json:string><json:string>Kimura et al., 1966</json:string><json:string>Pabo and Lewis, 1982</json:string><json:string>Bienz and Pelham, 1986</json:string><json:string>Cohen et al., 1988</json:string><json:string>Darn et al., 3987</json:string><json:string>Dynan and Tjian, 1985</json:string><json:string>Charnay et al., 1985</json:string><json:string>Jones et al., 1987</json:string><json:string>Flavell et al., 1985</json:string><json:string>Dignam et al. (1983)</json:string><json:string>McKnight and Kingsbury, 1982</json:string><json:string>Matthes et al., 1986</json:string><json:string>Anderson et al., 1985</json:string><json:string>Kreidberg and Kelly, 1966</json:string><json:string>de Vries et al., 1987</json:string><json:string>Johnson et al., 1967</json:string><json:string>Miyamoto et al., 1985</json:string><json:string>Bottazro et al., 1983</json:string><json:string>Mellon et al., 1981</json:string><json:string>Cohen et al., 1986</json:string><json:string>Jones et al., 1985, 1987</json:string><json:string>Diffley and Stillman (1986)</json:string><json:string>Jones et al., 1985</json:string><json:string>Saito et al., 1963</json:string><json:string>Carthew et al., 1985</json:string><json:string>Falkner and Zachau, 1984</json:string><json:string>Graves et al., 1986</json:string><json:string>Bienz and Pelham, 1966</json:string><json:string>Myers et al., 1986</json:string><json:string>Graves et al., 1985a, 1985b</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-J69FD1W8-R</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - cell biology</json:string><json:string>2 - biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - developmental biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - General Biochemistry, Genetics and Molecular Biology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1987</publicationDate><copyrightDate>1987</copyrightDate><doi><json:string>10.1016/0092-8674(87)90513-7</json:string></doi><id>523F85199F114B4B17AEEAF87EFEBEFCCE27C897</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-J69FD1W8-R/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-J69FD1W8-R/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-J69FD1W8-R/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">A multiplicity of CCAAT box-binding proteins</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1987</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">A multiplicity of CCAAT box-binding proteins</title><author xml:id="author-0000"><persName><forename type="first">Arnulf</forename><surname>Dorn</surname></persName><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Jacques</forename><surname>Bollekens</surname></persName><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Adrian</forename><surname>Staub</surname></persName><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Christophe</forename><surname>Benoist</surname></persName><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Diane</forename><surname>Mathis</surname></persName><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation></author><idno type="istex">523F85199F114B4B17AEEAF87EFEBEFCCE27C897</idno><idno type="ark">ark:/67375/6H6-J69FD1W8-R</idno><idno type="DOI">10.1016/0092-8674(87)90513-7</idno><idno type="PII">0092-8674(87)90513-7</idno></analytic><monogr><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="pISSN">0092-8674</idno><idno type="PII">S0092-8674(00)X0268-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1987"></date><biblScope unit="volume">50</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="863">863</biblScope><biblScope unit="page" to="872">872</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1987</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.</p></abstract></profileDesc><revisionDesc><change when="1987-07-10">Modified</change><change when="1987">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-J69FD1W8-R/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CELL</jid><aid>87905137</aid><ce:pii>0092-8674(87)90513-7</ce:pii><ce:doi>10.1016/0092-8674(87)90513-7</ce:doi><ce:copyright type="unknown" year="1987"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Article</ce:textfn></ce:dochead><ce:title>A multiplicity of CCAAT box-binding proteins</ce:title><ce:author-group><ce:author><ce:given-name>Arnulf</ce:given-name><ce:surname>Dorn</ce:surname></ce:author><ce:author><ce:given-name>Jacques</ce:given-name><ce:surname>Bollekens</ce:surname></ce:author><ce:author><ce:given-name>Adrian</ce:given-name><ce:surname>Staub</ce:surname></ce:author><ce:author><ce:given-name>Christophe</ce:given-name><ce:surname>Benoist</ce:surname></ce:author><ce:author><ce:given-name>Diane</ce:given-name><ce:surname>Mathis</ce:surname></ce:author><ce:affiliation><ce:textfn>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="29" month="4" year="1987"></ce:date-received><ce:date-revised day="10" month="7" year="1987"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>A multiplicity of CCAAT box-binding proteins</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>A multiplicity of CCAAT box-binding proteins</title></titleInfo><name type="personal"><namePart type="given">Arnulf</namePart><namePart type="family">Dorn</namePart><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jacques</namePart><namePart type="family">Bollekens</namePart><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Adrian</namePart><namePart type="family">Staub</namePart><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christophe</namePart><namePart type="family">Benoist</namePart><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Diane</namePart><namePart type="family">Mathis</namePart><affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes du C.N.R.S. Unité 184 de Biologie Moléculaire et de Génie Génétique de I'I.N.S.E.R.M. Institut de Chimie Biologique, Faculté de Médecine 67085 Strasbourg Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1987</dateIssued><dateModified encoding="w3cdtf">1987-07-10</dateModified><copyrightDate encoding="w3cdtf">1987</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.</abstract><note type="content">Section title: Article</note><relatedItem type="host"><titleInfo><title>Cell</title></titleInfo><titleInfo type="abbreviated"><title>CELL</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1987</dateIssued></originInfo><identifier type="ISSN">0092-8674</identifier><identifier type="PII">S0092-8674(00)X0268-1</identifier><part><date>1987</date><detail type="volume"><number>50</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="issue-pages"><start>819</start><end>994</end></extent><extent unit="pages"><start>863</start><end>872</end></extent></part></relatedItem><identifier type="istex">523F85199F114B4B17AEEAF87EFEBEFCCE27C897</identifier><identifier type="ark">ark:/67375/6H6-J69FD1W8-R</identifier><identifier type="DOI">10.1016/0092-8674(87)90513-7</identifier><identifier type="PII">0092-8674(87)90513-7</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-J69FD1W8-R/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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