Biological Microchips with Hydrogel-Immobilized Nucleic Acids, Proteins, and Other Compounds: Properties and Applications in Genomics
Identifieur interne : 002281 ( Istex/Corpus ); précédent : 002280; suivant : 002282Biological Microchips with Hydrogel-Immobilized Nucleic Acids, Proteins, and Other Compounds: Properties and Applications in Genomics
Auteurs : V. E. Barsky ; A. M. Kolchinsky ; Yu. P. Lysov ; A. D. MirzabekovSource :
- Molecular Biology [ 0026-8933 ] ; 2002-07-01.
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Abstract
Abstract: The MAGIChip (MicroArrays of Gel-Immobilized Compounds on a chip) consists of an array of hydrophilic gel pads fixed on a hydrophobic glass surface. These pads of several picoliters to several nanoliters in volume contain gel-immobilized nucleic acids, proteins, and other compounds, as well as live cells. They are used to conduct chemical and enzymatic reactions with the immobilized compounds or samples bound to them. In the latter case, nucleic acid fragments can be hybridized, modified, and fractionated within the gel pads. The main procedures required to analyze nucleic acid sequences (PCR, detachment of primers and PCR-amplified products from a substrate, hybridization, ligation, and others) can be also performed within the microchip pads. A flexible, multipurpose, and inexpensive system has been developed to register the processes on a microchip. The system provides unique possibilities for research and biomedical applications, allowing one to register both equilibrium states and the course of reaction in real time. The system is applied to analyze both kinetic and thermodynamic characteristics of molecular interaction in the duplexes formed between nucleic acids and the probes immobilized within the microchip gel pads. Owing to the effect of stacking interaction of nucleic acids, the use of short oligonucleotides extends the possibilities of microchips for analysis of nucleic acid sequences, allowing one to employ the MALDI-TOF mass spectrometry to analyze the hybridization data. The specialized MAGIChips has been successfully applied to reveal single-nucleotide polymorphism of many biologically significant genes, to identify bacteria and viruses, to detect toxins and characterize the genes of pathogenic bacteria responsible for drug resistance, and to study translocations in the human genome. On the basis of the MAGIChip, protein microchips have been created, containing immobilized antibodies, antigens, enzymes, and many other substances, as well as microchips with gel-immobilized live cells.
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DOI: 10.1023/A:1019804005711
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Mirzabekov</name><affiliation><mods:affiliation>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: amir@eimb.ru</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Biology</title><title level="j" type="abbrev">Molecular Biology</title><idno type="ISSN">0026-8933</idno><idno type="eISSN">1608-3245</idno><imprint><publisher>Kluwer Academic Publishers-Plenum Publishers</publisher><pubPlace>New York</pubPlace><date type="published" when="2002-07-01">2002-07-01</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="437">437</biblScope><biblScope unit="page" to="455">455</biblScope></imprint><idno type="ISSN">0026-8933</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0026-8933</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>biological microchips</term><term>genomics</term><term>nucleic acids</term><term>proteins</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The MAGIChip (MicroArrays of Gel-Immobilized Compounds on a chip) consists of an array of hydrophilic gel pads fixed on a hydrophobic glass surface. These pads of several picoliters to several nanoliters in volume contain gel-immobilized nucleic acids, proteins, and other compounds, as well as live cells. They are used to conduct chemical and enzymatic reactions with the immobilized compounds or samples bound to them. In the latter case, nucleic acid fragments can be hybridized, modified, and fractionated within the gel pads. The main procedures required to analyze nucleic acid sequences (PCR, detachment of primers and PCR-amplified products from a substrate, hybridization, ligation, and others) can be also performed within the microchip pads. A flexible, multipurpose, and inexpensive system has been developed to register the processes on a microchip. The system provides unique possibilities for research and biomedical applications, allowing one to register both equilibrium states and the course of reaction in real time. The system is applied to analyze both kinetic and thermodynamic characteristics of molecular interaction in the duplexes formed between nucleic acids and the probes immobilized within the microchip gel pads. Owing to the effect of stacking interaction of nucleic acids, the use of short oligonucleotides extends the possibilities of microchips for analysis of nucleic acid sequences, allowing one to employ the MALDI-TOF mass spectrometry to analyze the hybridization data. The specialized MAGIChips has been successfully applied to reveal single-nucleotide polymorphism of many biologically significant genes, to identify bacteria and viruses, to detect toxins and characterize the genes of pathogenic bacteria responsible for drug resistance, and to study translocations in the human genome. On the basis of the MAGIChip, protein microchips have been created, containing immobilized antibodies, antigens, enzymes, and many other substances, as well as microchips with gel-immobilized live cells.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>V. E. Barsky</name><affiliations><json:string>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia</json:string><json:string>E-mail: amir@eimb.ru</json:string></affiliations></json:item><json:item><name>A. M. Kolchinsky</name><affiliations><json:string>Front Health Line, Champagne, IL, USA</json:string></affiliations></json:item><json:item><name>Yu. P. Lysov</name><affiliations><json:string>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia</json:string><json:string>E-mail: amir@eimb.ru</json:string></affiliations></json:item><json:item><name>A. D. 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These pads of several picoliters to several nanoliters in volume contain gel-immobilized nucleic acids, proteins, and other compounds, as well as live cells. They are used to conduct chemical and enzymatic reactions with the immobilized compounds or samples bound to them. In the latter case, nucleic acid fragments can be hybridized, modified, and fractionated within the gel pads. The main procedures required to analyze nucleic acid sequences (PCR, detachment of primers and PCR-amplified products from a substrate, hybridization, ligation, and others) can be also performed within the microchip pads. A flexible, multipurpose, and inexpensive system has been developed to register the processes on a microchip. The system provides unique possibilities for research and biomedical applications, allowing one to register both equilibrium states and the course of reaction in real time. The system is applied to analyze both kinetic and thermodynamic characteristics of molecular interaction in the duplexes formed between nucleic acids and the probes immobilized within the microchip gel pads. Owing to the effect of stacking interaction of nucleic acids, the use of short oligonucleotides extends the possibilities of microchips for analysis of nucleic acid sequences, allowing one to employ the MALDI-TOF mass spectrometry to analyze the hybridization data. The specialized MAGIChips has been successfully applied to reveal single-nucleotide polymorphism of many biologically significant genes, to identify bacteria and viruses, to detect toxins and characterize the genes of pathogenic bacteria responsible for drug resistance, and to study translocations in the human genome. 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ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The MAGIChip (MicroArrays of Gel-Immobilized Compounds on a chip) consists of an array of hydrophilic gel pads fixed on a hydrophobic glass surface. These pads of several picoliters to several nanoliters in volume contain gel-immobilized nucleic acids, proteins, and other compounds, as well as live cells. They are used to conduct chemical and enzymatic reactions with the immobilized compounds or samples bound to them. In the latter case, nucleic acid fragments can be hybridized, modified, and fractionated within the gel pads. The main procedures required to analyze nucleic acid sequences (PCR, detachment of primers and PCR-amplified products from a substrate, hybridization, ligation, and others) can be also performed within the microchip pads. A flexible, multipurpose, and inexpensive system has been developed to register the processes on a microchip. The system provides unique possibilities for research and biomedical applications, allowing one to register both equilibrium states and the course of reaction in real time. The system is applied to analyze both kinetic and thermodynamic characteristics of molecular interaction in the duplexes formed between nucleic acids and the probes immobilized within the microchip gel pads. Owing to the effect of stacking interaction of nucleic acids, the use of short oligonucleotides extends the possibilities of microchips for analysis of nucleic acid sequences, allowing one to employ the MALDI-TOF mass spectrometry to analyze the hybridization data. The specialized MAGIChips has been successfully applied to reveal single-nucleotide polymorphism of many biologically significant genes, to identify bacteria and viruses, to detect toxins and characterize the genes of pathogenic bacteria responsible for drug resistance, and to study translocations in the human genome. On the basis of the MAGIChip, protein microchips have been created, containing immobilized antibodies, antigens, enzymes, and many other substances, as well as microchips with gel-immobilized live cells.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><item><term>biological microchips</term></item><item><term>genomics</term></item><item><term>nucleic acids</term></item><item><term>proteins</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Human Genetics</term></item><item><term>Life Sciences, general</term></item><item><term>Biochemistry, general</term></item></list></keywords></textClass></profileDesc><revisionDesc><change 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DisplayOrder="Western"><GivenName>V.</GivenName><GivenName>E.</GivenName><FamilyName>Barsky</FamilyName></AuthorName><Contact><Email>amir@eimb.ru</Email></Contact></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>A.</GivenName><GivenName>M.</GivenName><FamilyName>Kolchinsky</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Yu.</GivenName><GivenName>P.</GivenName><FamilyName>Lysov</FamilyName></AuthorName><Contact><Email>amir@eimb.ru</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>A.</GivenName><GivenName>D.</GivenName><FamilyName>Mirzabekov</FamilyName></AuthorName><Contact><Email>amir@eimb.ru</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Engelhardt Institute of Molecular Biology</OrgDivision><OrgName>Russian Academy of Sciences</OrgName><OrgAddress><City>Moscow</City><Postcode>119991</Postcode><Country>Russia</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgName>Front Health Line</OrgName><OrgAddress><City>Champagne</City><State>IL</State><Country>USA</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>The MAGIChip (MicroArrays of Gel-Immobilized Compounds on a chip) consists of an array of hydrophilic gel pads fixed on a hydrophobic glass surface. These pads of several picoliters to several nanoliters in volume contain gel-immobilized nucleic acids, proteins, and other compounds, as well as live cells. They are used to conduct chemical and enzymatic reactions with the immobilized compounds or samples bound to them. In the latter case, nucleic acid fragments can be hybridized, modified, and fractionated within the gel pads. The main procedures required to analyze nucleic acid sequences (PCR, detachment of primers and PCR-amplified products from a substrate, hybridization, ligation, and others) can be also performed within the microchip pads. A flexible, multipurpose, and inexpensive system has been developed to register the processes on a microchip. The system provides unique possibilities for research and biomedical applications, allowing one to register both equilibrium states and the course of reaction in real time. The system is applied to analyze both kinetic and thermodynamic characteristics of molecular interaction in the duplexes formed between nucleic acids and the probes immobilized within the microchip gel pads. Owing to the effect of stacking interaction of nucleic acids, the use of short oligonucleotides extends the possibilities of microchips for analysis of nucleic acid sequences, allowing one to employ the MALDI-TOF mass spectrometry to analyze the hybridization data. The specialized MAGIChips has been successfully applied to reveal single-nucleotide polymorphism of many biologically significant genes, to identify bacteria and viruses, to detect toxins and characterize the genes of pathogenic bacteria responsible for drug resistance, and to study translocations in the human genome. On the basis of the MAGIChip, protein microchips have been created, containing immobilized antibodies, antigens, enzymes, and many other substances, as well as microchips with gel-immobilized live cells.</Para></Abstract><KeywordGroup Language="En"><Keyword>biological microchips</Keyword><Keyword>genomics</Keyword><Keyword>nucleic acids</Keyword><Keyword>proteins</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Biological Microchips with Hydrogel-Immobilized Nucleic Acids, Proteins, and Other Compounds: Properties and Applications in Genomics</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Biological Microchips with Hydrogel-Immobilized Nucleic Acids, Proteins, and Other Compounds: Properties and Applications in Genomics</title></titleInfo><name type="personal"><namePart type="given">V.</namePart><namePart type="given">E.</namePart><namePart type="family">Barsky</namePart><affiliation>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia</affiliation><affiliation>E-mail: amir@eimb.ru</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="given">M.</namePart><namePart type="family">Kolchinsky</namePart><affiliation>Front Health Line, Champagne, IL, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yu.</namePart><namePart type="given">P.</namePart><namePart type="family">Lysov</namePart><affiliation>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia</affiliation><affiliation>E-mail: amir@eimb.ru</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="given">D.</namePart><namePart type="family">Mirzabekov</namePart><affiliation>Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia</affiliation><affiliation>E-mail: amir@eimb.ru</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Kluwer Academic Publishers-Plenum Publishers</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">2002-07-01</dateIssued><copyrightDate encoding="w3cdtf">2002</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: The MAGIChip (MicroArrays of Gel-Immobilized Compounds on a chip) consists of an array of hydrophilic gel pads fixed on a hydrophobic glass surface. These pads of several picoliters to several nanoliters in volume contain gel-immobilized nucleic acids, proteins, and other compounds, as well as live cells. They are used to conduct chemical and enzymatic reactions with the immobilized compounds or samples bound to them. In the latter case, nucleic acid fragments can be hybridized, modified, and fractionated within the gel pads. The main procedures required to analyze nucleic acid sequences (PCR, detachment of primers and PCR-amplified products from a substrate, hybridization, ligation, and others) can be also performed within the microchip pads. A flexible, multipurpose, and inexpensive system has been developed to register the processes on a microchip. The system provides unique possibilities for research and biomedical applications, allowing one to register both equilibrium states and the course of reaction in real time. The system is applied to analyze both kinetic and thermodynamic characteristics of molecular interaction in the duplexes formed between nucleic acids and the probes immobilized within the microchip gel pads. Owing to the effect of stacking interaction of nucleic acids, the use of short oligonucleotides extends the possibilities of microchips for analysis of nucleic acid sequences, allowing one to employ the MALDI-TOF mass spectrometry to analyze the hybridization data. The specialized MAGIChips has been successfully applied to reveal single-nucleotide polymorphism of many biologically significant genes, to identify bacteria and viruses, to detect toxins and characterize the genes of pathogenic bacteria responsible for drug resistance, and to study translocations in the human genome. On the basis of the MAGIChip, protein microchips have been created, containing immobilized antibodies, antigens, enzymes, and many other substances, as well as microchips with gel-immobilized live cells.</abstract><subject lang="en"><topic>biological microchips</topic><topic>genomics</topic><topic>nucleic acids</topic><topic>proteins</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Biology</title></titleInfo><titleInfo type="abbreviated"><title>Molecular Biology</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2002-07-01</dateIssued><copyrightDate encoding="w3cdtf">2002</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Human Genetics</topic><topic>Life Sciences, general</topic><topic>Biochemistry, general</topic></subject><identifier type="ISSN">0026-8933</identifier><identifier type="eISSN">1608-3245</identifier><identifier type="JournalID">11008</identifier><identifier type="IssueArticleCount">27</identifier><identifier type="VolumeIssueCount">8</identifier><part><date>2002</date><detail type="volume"><number>36</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="pages"><start>437</start><end>455</end></extent></part><recordInfo><recordOrigin>MAIK “Nauka/Interperiodica”, 2002</recordOrigin></recordInfo></relatedItem><identifier type="istex">CC83F15EC877E16B5F3F0E58C00C60C585FDD438</identifier><identifier type="ark">ark:/67375/VQC-XKCR6F4H-Z</identifier><identifier type="DOI">10.1023/A:1019804005711</identifier><identifier type="ArticleID">452108</identifier><identifier type="ArticleID">Art1</identifier><accessCondition type="use and reproduction" contentType="copyright">MAIK “Nauka/Interperiodica”, 2002</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>MAIK “Nauka/Interperiodica”, 2002</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-XKCR6F4H-Z/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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