Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells
Identifieur interne : 002122 ( Istex/Corpus ); précédent : 002121; suivant : 002123Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells
Auteurs : Tyler J. Curiel ; Deborah R. Cook ; Christoph Bogedain ; Wolfgang Jilg ; Gail S. Harrison ; Matt Cotten ; David T. Curiel ; Ernst WagnerSource :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing efficient foreign gene expression in B-LCL using biotinylated adenovirus as an endosome-disrupting agent. Plasmid DNA is coupled to the exterior of viral particles by streptavidin-polylysine chimeric proteins. Up to 67% of B-LCL may be induced to express foreign genes in vitro in transient expression systems, and gene expression lasts for at least 17 days. We have expressed firefly luciferase, β-galactosidase (β-gal), chloramphenicol acetyltransferase, HIV gag, and env genes, as well as infectious HIV, and the EBV-specific BZLF gene in B-LCL with this system. In vivo delivery of a β-gal reporter gene to B-LCL was documented in a SCID mouse model. Potential applications include study of genetic regulation of EBV infection and transformation events, study of potential gene therapies for EBV-related B-cell tumors, and production of antigen-presenting cells for use in immunologic assays. Because of the high percentage of cells transformed and the length of foreign gene expression, the possibility of examining foreign gene expression in transient assays, without selection for clonal populations, exists.
Url:
DOI: 10.1006/viro.1994.1069
Links to Exploration step
ISTEX:FE3005B4FF86BD0822D65463D03CEB8F61B2D6A4Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells</title><author><name sortKey="Curiel, Tyler J" sort="Curiel, Tyler J" uniqKey="Curiel T" first="Tyler J." last="Curiel">Tyler J. Curiel</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Cook, Deborah R" sort="Cook, Deborah R" uniqKey="Cook D" first="Deborah R." last="Cook">Deborah R. Cook</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Bogedain, Christoph" sort="Bogedain, Christoph" uniqKey="Bogedain C" first="Christoph" last="Bogedain">Christoph Bogedain</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Jilg, Wolfgang" sort="Jilg, Wolfgang" uniqKey="Jilg W" first="Wolfgang" last="Jilg">Wolfgang Jilg</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Harrison, Gail S" sort="Harrison, Gail S" uniqKey="Harrison G" first="Gail S." last="Harrison">Gail S. Harrison</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Cotten, Matt" sort="Cotten, Matt" uniqKey="Cotten M" first="Matt" last="Cotten">Matt Cotten</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Curiel, David T" sort="Curiel, David T" uniqKey="Curiel D" first="David T." last="Curiel">David T. Curiel</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Wagner, Ernst" sort="Wagner, Ernst" uniqKey="Wagner E" first="Ernst" last="Wagner">Ernst Wagner</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FE3005B4FF86BD0822D65463D03CEB8F61B2D6A4</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1006/viro.1994.1069</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-076RR8RW-C/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">002122</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002122</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells</title><author><name sortKey="Curiel, Tyler J" sort="Curiel, Tyler J" uniqKey="Curiel T" first="Tyler J." last="Curiel">Tyler J. Curiel</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Cook, Deborah R" sort="Cook, Deborah R" uniqKey="Cook D" first="Deborah R." last="Cook">Deborah R. Cook</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Bogedain, Christoph" sort="Bogedain, Christoph" uniqKey="Bogedain C" first="Christoph" last="Bogedain">Christoph Bogedain</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Jilg, Wolfgang" sort="Jilg, Wolfgang" uniqKey="Jilg W" first="Wolfgang" last="Jilg">Wolfgang Jilg</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Harrison, Gail S" sort="Harrison, Gail S" uniqKey="Harrison G" first="Gail S." last="Harrison">Gail S. Harrison</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Cotten, Matt" sort="Cotten, Matt" uniqKey="Cotten M" first="Matt" last="Cotten">Matt Cotten</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Curiel, David T" sort="Curiel, David T" uniqKey="Curiel D" first="David T." last="Curiel">David T. Curiel</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author><author><name sortKey="Wagner, Ernst" sort="Wagner, Ernst" uniqKey="Wagner E" first="Ernst" last="Wagner">Ernst Wagner</name><affiliation><mods:affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">198</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="577">577</biblScope><biblScope unit="page" to="585">585</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing efficient foreign gene expression in B-LCL using biotinylated adenovirus as an endosome-disrupting agent. Plasmid DNA is coupled to the exterior of viral particles by streptavidin-polylysine chimeric proteins. Up to 67% of B-LCL may be induced to express foreign genes in vitro in transient expression systems, and gene expression lasts for at least 17 days. We have expressed firefly luciferase, β-galactosidase (β-gal), chloramphenicol acetyltransferase, HIV gag, and env genes, as well as infectious HIV, and the EBV-specific BZLF gene in B-LCL with this system. In vivo delivery of a β-gal reporter gene to B-LCL was documented in a SCID mouse model. Potential applications include study of genetic regulation of EBV infection and transformation events, study of potential gene therapies for EBV-related B-cell tumors, and production of antigen-presenting cells for use in immunologic assays. Because of the high percentage of cells transformed and the length of foreign gene expression, the possibility of examining foreign gene expression in transient assays, without selection for clonal populations, exists.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Tyler J. Curiel</name><affiliations><json:string>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</json:string></affiliations></json:item><json:item><name>Deborah R. Cook</name><affiliations><json:string>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</json:string></affiliations></json:item><json:item><name>Christoph Bogedain</name><affiliations><json:string>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</json:string></affiliations></json:item><json:item><name>Wolfgang Jilg</name><affiliations><json:string>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</json:string></affiliations></json:item><json:item><name>Gail S. Harrison</name><affiliations><json:string>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</json:string></affiliations></json:item><json:item><name>Matt Cotten</name><affiliations><json:string>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</json:string></affiliations></json:item><json:item><name>David T. Curiel</name><affiliations><json:string>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</json:string></affiliations></json:item><json:item><name>Ernst Wagner</name><affiliations><json:string>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-076RR8RW-C</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing efficient foreign gene expression in B-LCL using biotinylated adenovirus as an endosome-disrupting agent. Plasmid DNA is coupled to the exterior of viral particles by streptavidin-polylysine chimeric proteins. Up to 67% of B-LCL may be induced to express foreign genes in vitro in transient expression systems, and gene expression lasts for at least 17 days. We have expressed firefly luciferase, β-galactosidase (β-gal), chloramphenicol acetyltransferase, HIV gag, and env genes, as well as infectious HIV, and the EBV-specific BZLF gene in B-LCL with this system. In vivo delivery of a β-gal reporter gene to B-LCL was documented in a SCID mouse model. Potential applications include study of genetic regulation of EBV infection and transformation events, study of potential gene therapies for EBV-related B-cell tumors, and production of antigen-presenting cells for use in immunologic assays. Because of the high percentage of cells transformed and the length of foreign gene expression, the possibility of examining foreign gene expression in transient assays, without selection for clonal populations, exists.</abstract><qualityIndicators><score>4.558</score><pdfWordCount>0</pdfWordCount><pdfCharCount>0</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>611 x 802 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>209</abstractWordCount><abstractCharCount>1443</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells</title><pmid><json:string>8291240</json:string></pmid><pii><json:string>S0042-6822(84)71069-5</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1994</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0172-9</json:string></pii><volume>198</volume><issue>2</issue><pages><first>577</first><last>585</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName></persName><placeName></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-076RR8RW-C</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1994</publicationDate><copyrightDate>1994</copyrightDate><doi><json:string>10.1006/viro.1994.1069</json:string></doi><id>FE3005B4FF86BD0822D65463D03CEB8F61B2D6A4</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-076RR8RW-C/fulltext.pdf</uri></json:item><json:item><extension>ocr</extension><original>false</original><mimetype>text/ocr</mimetype><quality><totalToken>6830</totalToken><correct>5858</correct><rate>79.3107463248366</rate><misspelled>990</misspelled></quality><langDetect><reliable>true</reliable><languages><json:item><score>884</score><code>en</code><name>ENGLISH</name><percent>99</percent></json:item></languages></langDetect><uri>https://api.istex.fr/ark:/67375/6H6-076RR8RW-C/fulltext.ocr</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-076RR8RW-C/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-076RR8RW-C/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1994 Academic Press</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1994</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Regular Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells</title><author xml:id="author-0000"><persName><forename type="first">Tyler J.</forename><surname>Curiel</surname></persName><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Deborah R.</forename><surname>Cook</surname></persName><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Christoph</forename><surname>Bogedain</surname></persName><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Wolfgang</forename><surname>Jilg</surname></persName><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Gail S.</forename><surname>Harrison</surname></persName><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Matt</forename><surname>Cotten</surname></persName><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation></author><author xml:id="author-0006"><persName><forename type="first">David T.</forename><surname>Curiel</surname></persName><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation></author><author xml:id="author-0007"><persName><forename type="first">Ernst</forename><surname>Wagner</surname></persName><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation></author><idno type="istex">FE3005B4FF86BD0822D65463D03CEB8F61B2D6A4</idno><idno type="ark">ark:/67375/6H6-076RR8RW-C</idno><idno type="DOI">10.1006/viro.1994.1069</idno><idno type="PII">S0042-6822(84)71069-5</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0172-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994"></date><biblScope unit="volume">198</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="577">577</biblScope><biblScope unit="page" to="585">585</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing efficient foreign gene expression in B-LCL using biotinylated adenovirus as an endosome-disrupting agent. Plasmid DNA is coupled to the exterior of viral particles by streptavidin-polylysine chimeric proteins. Up to 67% of B-LCL may be induced to express foreign genes in vitro in transient expression systems, and gene expression lasts for at least 17 days. We have expressed firefly luciferase, β-galactosidase (β-gal), chloramphenicol acetyltransferase, HIV gag, and env genes, as well as infectious HIV, and the EBV-specific BZLF gene in B-LCL with this system. In vivo delivery of a β-gal reporter gene to B-LCL was documented in a SCID mouse model. Potential applications include study of genetic regulation of EBV infection and transformation events, study of potential gene therapies for EBV-related B-cell tumors, and production of antigen-presenting cells for use in immunologic assays. Because of the high percentage of cells transformed and the length of foreign gene expression, the possibility of examining foreign gene expression in transient assays, without selection for clonal populations, exists.</p></abstract></profileDesc><revisionDesc><change when="1994">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-076RR8RW-C/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier converted-article found"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YVIRO</jid><aid>71069</aid><ce:pii>S0042-6822(84)71069-5</ce:pii><ce:doi>10.1006/viro.1994.1069</ce:doi><ce:copyright type="full-transfer" year="1994">Academic Press</ce:copyright></item-info><head><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells</ce:title><ce:author-group><ce:author><ce:given-name>Tyler J.</ce:given-name><ce:surname>Curiel</ce:surname></ce:author><ce:author><ce:given-name>Deborah R.</ce:given-name><ce:surname>Cook</ce:surname></ce:author><ce:author><ce:given-name>Christoph</ce:given-name><ce:surname>Bogedain</ce:surname></ce:author><ce:author><ce:given-name>Wolfgang</ce:given-name><ce:surname>Jilg</ce:surname></ce:author><ce:author><ce:given-name>Gail S.</ce:given-name><ce:surname>Harrison</ce:surname></ce:author><ce:author><ce:given-name>Matt</ce:given-name><ce:surname>Cotten</ce:surname></ce:author><ce:author><ce:given-name>David T.</ce:given-name><ce:surname>Curiel</ce:surname></ce:author><ce:author><ce:given-name>Ernst</ce:given-name><ce:surname>Wagner</ce:surname></ce:author><ce:affiliation><ce:textfn>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing efficient foreign gene expression in B-LCL using biotinylated adenovirus as an endosome-disrupting agent. Plasmid DNA is coupled to the exterior of viral particles by streptavidin-polylysine chimeric proteins. Up to 67% of B-LCL may be induced to express foreign genes <ce:italic>in vitro</ce:italic> in transient expression systems, and gene expression lasts for at least 17 days. We have expressed firefly luciferase, β-galactosidase (β-gal), chloramphenicol acetyltransferase, HIV <ce:italic>gag,</ce:italic> and <ce:italic>env</ce:italic> genes, as well as infectious HIV, and the EBV-specific BZLF gene in B-LCL with this system. <ce:italic>In vivo</ce:italic> delivery of a β-gal reporter gene to B-LCL was documented in a SCID mouse model. Potential applications include study of genetic regulation of EBV infection and transformation events, study of potential gene therapies for EBV-related B-cell tumors, and production of antigen-presenting cells for use in immunologic assays. Because of the high percentage of cells transformed and the length of foreign gene expression, the possibility of examining foreign gene expression in transient assays, without selection for clonal populations, exists.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells</title></titleInfo><name type="personal"><namePart type="given">Tyler J.</namePart><namePart type="family">Curiel</namePart><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Deborah R.</namePart><namePart type="family">Cook</namePart><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christoph</namePart><namePart type="family">Bogedain</namePart><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wolfgang</namePart><namePart type="family">Jilg</namePart><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gail S.</namePart><namePart type="family">Harrison</namePart><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Matt</namePart><namePart type="family">Cotten</namePart><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David T.</namePart><namePart type="family">Curiel</namePart><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ernst</namePart><namePart type="family">Wagner</namePart><affiliation>Divisions of Infectious Disease and Medical Oncology, University of Colorado Health Sciences Center, Denver, Colorado 80262; University of North Carolina at Chapel Hill, CB 7020, 724 Burnett-Womack Building, Chapel Hill, North Carolina 27599-7020; Max von Pettenkofer Institut, D-8000 Munich, Germany; and Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing efficient foreign gene expression in B-LCL using biotinylated adenovirus as an endosome-disrupting agent. Plasmid DNA is coupled to the exterior of viral particles by streptavidin-polylysine chimeric proteins. Up to 67% of B-LCL may be induced to express foreign genes in vitro in transient expression systems, and gene expression lasts for at least 17 days. We have expressed firefly luciferase, β-galactosidase (β-gal), chloramphenicol acetyltransferase, HIV gag, and env genes, as well as infectious HIV, and the EBV-specific BZLF gene in B-LCL with this system. In vivo delivery of a β-gal reporter gene to B-LCL was documented in a SCID mouse model. Potential applications include study of genetic regulation of EBV infection and transformation events, study of potential gene therapies for EBV-related B-cell tumors, and production of antigen-presenting cells for use in immunologic assays. Because of the high percentage of cells transformed and the length of foreign gene expression, the possibility of examining foreign gene expression in transient assays, without selection for clonal populations, exists.</abstract><note type="content">Section title: Regular Article</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0172-9</identifier><part><date>1994</date><detail type="volume"><number>198</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>415</start><end>756</end></extent><extent unit="pages"><start>577</start><end>585</end></extent></part></relatedItem><identifier type="istex">FE3005B4FF86BD0822D65463D03CEB8F61B2D6A4</identifier><identifier type="ark">ark:/67375/6H6-076RR8RW-C</identifier><identifier type="DOI">10.1006/viro.1994.1069</identifier><identifier type="PII">S0042-6822(84)71069-5</identifier><accessCondition type="use and reproduction" contentType="copyright">©1994 Academic Press</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Academic Press, ©1994</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-076RR8RW-C/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002122 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 002122 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:FE3005B4FF86BD0822D65463D03CEB8F61B2D6A4
|texte= Efficient Foreign Gene Expression in Epstein-Barr Virus-Transformed Human B-Cells
}}
| This area was generated with Dilib version V0.6.33. |  |