Serveur d'exploration MERS

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas

Identifieur interne : 002052 ( Istex/Corpus ); précédent : 002051; suivant : 002053

Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas

Auteurs : Ulla M. Wewer ; Donald R. Gerecke ; Marian E. Durkin ; Kenneth S. Kurtz ; Marie-Genevieve Mattei ; Marie-France Champliaud ; Robert E. Burgeson ; Reidar Albrechtsen

Source :

RBID : ISTEX:57DED64C05F928A8500CD1F4A2B650F1745C2FF5

Abstract

Abstract: Overlapping cDNA clones that encode the full-length human laminin β2 chain, formerly called the S chain, were isolated. The cDNA of 5680 nt contains a 5391-nt open reading frame encoding 1797 amino acids. At the amino terminus is a 32-amino-acid signal peptide that is followed by the mature β2 chain polypeptide of 1765 amino acids with a calculated molecular mass of 192,389 Da. The human β2 chain is predicted to have all of the seven structural domains typical of the β chains of laminin, including the short cysteine-rich α region. The amino acid sequence of human β2 chain showed 86.1% sequence identity to the rat β2 chain, 50.0% to the human β1 chain, and 36.3% to the human β3 chain. The greatest sequence identity was in domains VI, V, and III. The sequence of a 24-amino-acid peptide fragment isolated from the β2 chain of laminin purified from human amniotic basement membrane matched the sequence predicted from the cDNA, confirming that the cDNA encodes human β2 laminin. The cDNA was used to assign the gene (LAMB2) to human chromosome 3p21 by in situ hybridization. It is not linked to genes for human laminin chains α1, β1, and γ1 or other known laminin genes. Immunostaining showed that the β2 chain is localized to the smooth muscle basement membranes of the arteries, while the homologous β1 chain is confined to the subendothelial basement membranes. The β2 chain was found in the basement membranes of ovarian carcinomas but not colon carcinomas. These results indicate that the expression of the β2 chain gene is tightly regulated in normal human tissues and in disease.

Url:
DOI: 10.1006/geno.1994.1612

Links to Exploration step

ISTEX:57DED64C05F928A8500CD1F4A2B650F1745C2FF5

Le document en format XML

<record>
<TEI wicri:istexFullTextTei="biblStruct">
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas</title>
<author>
<name sortKey="Wewer, Ulla M" sort="Wewer, Ulla M" uniqKey="Wewer U" first="Ulla M." last="Wewer">Ulla M. Wewer</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Gerecke, Donald R" sort="Gerecke, Donald R" uniqKey="Gerecke D" first="Donald R." last="Gerecke">Donald R. Gerecke</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Durkin, Marian E" sort="Durkin, Marian E" uniqKey="Durkin M" first="Marian E." last="Durkin">Marian E. Durkin</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Kurtz, Kenneth S" sort="Kurtz, Kenneth S" uniqKey="Kurtz K" first="Kenneth S." last="Kurtz">Kenneth S. Kurtz</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Mattei, Marie Genevieve" sort="Mattei, Marie Genevieve" uniqKey="Mattei M" first="Marie-Genevieve" last="Mattei">Marie-Genevieve Mattei</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Champliaud, Marie France" sort="Champliaud, Marie France" uniqKey="Champliaud M" first="Marie-France" last="Champliaud">Marie-France Champliaud</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Burgeson, Robert E" sort="Burgeson, Robert E" uniqKey="Burgeson R" first="Robert E." last="Burgeson">Robert E. Burgeson</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Albrechtsen, Reidar" sort="Albrechtsen, Reidar" uniqKey="Albrechtsen R" first="Reidar" last="Albrechtsen">Reidar Albrechtsen</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:57DED64C05F928A8500CD1F4A2B650F1745C2FF5</idno>
<date when="1994" year="1994">1994</date>
<idno type="doi">10.1006/geno.1994.1612</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-SMK5QDD8-6/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002052</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002052</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title level="a" type="main" xml:lang="en">Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas</title>
<author>
<name sortKey="Wewer, Ulla M" sort="Wewer, Ulla M" uniqKey="Wewer U" first="Ulla M." last="Wewer">Ulla M. Wewer</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Gerecke, Donald R" sort="Gerecke, Donald R" uniqKey="Gerecke D" first="Donald R." last="Gerecke">Donald R. Gerecke</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Durkin, Marian E" sort="Durkin, Marian E" uniqKey="Durkin M" first="Marian E." last="Durkin">Marian E. Durkin</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Kurtz, Kenneth S" sort="Kurtz, Kenneth S" uniqKey="Kurtz K" first="Kenneth S." last="Kurtz">Kenneth S. Kurtz</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Mattei, Marie Genevieve" sort="Mattei, Marie Genevieve" uniqKey="Mattei M" first="Marie-Genevieve" last="Mattei">Marie-Genevieve Mattei</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Champliaud, Marie France" sort="Champliaud, Marie France" uniqKey="Champliaud M" first="Marie-France" last="Champliaud">Marie-France Champliaud</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Burgeson, Robert E" sort="Burgeson, Robert E" uniqKey="Burgeson R" first="Robert E." last="Burgeson">Robert E. Burgeson</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
<author>
<name sortKey="Albrechtsen, Reidar" sort="Albrechtsen, Reidar" uniqKey="Albrechtsen R" first="Reidar" last="Albrechtsen">Reidar Albrechtsen</name>
<affiliation>
<mods:affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series>
<title level="j">Genomics</title>
<title level="j" type="abbrev">YGENO</title>
<idno type="ISSN">0888-7543</idno>
<imprint>
<publisher>ELSEVIER</publisher>
<date type="published" when="1994">1994</date>
<biblScope unit="volume">24</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="243">243</biblScope>
<biblScope unit="page" to="252">252</biblScope>
</imprint>
<idno type="ISSN">0888-7543</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt>
<idno type="ISSN">0888-7543</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass></textClass>
<langUsage>
<language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">Abstract: Overlapping cDNA clones that encode the full-length human laminin β2 chain, formerly called the S chain, were isolated. The cDNA of 5680 nt contains a 5391-nt open reading frame encoding 1797 amino acids. At the amino terminus is a 32-amino-acid signal peptide that is followed by the mature β2 chain polypeptide of 1765 amino acids with a calculated molecular mass of 192,389 Da. The human β2 chain is predicted to have all of the seven structural domains typical of the β chains of laminin, including the short cysteine-rich α region. The amino acid sequence of human β2 chain showed 86.1% sequence identity to the rat β2 chain, 50.0% to the human β1 chain, and 36.3% to the human β3 chain. The greatest sequence identity was in domains VI, V, and III. The sequence of a 24-amino-acid peptide fragment isolated from the β2 chain of laminin purified from human amniotic basement membrane matched the sequence predicted from the cDNA, confirming that the cDNA encodes human β2 laminin. The cDNA was used to assign the gene (LAMB2) to human chromosome 3p21 by in situ hybridization. It is not linked to genes for human laminin chains α1, β1, and γ1 or other known laminin genes. Immunostaining showed that the β2 chain is localized to the smooth muscle basement membranes of the arteries, while the homologous β1 chain is confined to the subendothelial basement membranes. The β2 chain was found in the basement membranes of ovarian carcinomas but not colon carcinomas. These results indicate that the expression of the β2 chain gene is tightly regulated in normal human tissues and in disease.</div>
</front>
</TEI>
<istex>
<corpusName>elsevier</corpusName>
<author>
<json:item>
<name>Ulla M. Wewer</name>
<affiliations>
<json:string>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</json:string>
</affiliations>
</json:item>
<json:item>
<name>Donald R. Gerecke</name>
<affiliations>
<json:string>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</json:string>
</affiliations>
</json:item>
<json:item>
<name>Marian E. Durkin</name>
<affiliations>
<json:string>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</json:string>
</affiliations>
</json:item>
<json:item>
<name>Kenneth S. Kurtz</name>
<affiliations>
<json:string>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</json:string>
</affiliations>
</json:item>
<json:item>
<name>Marie-Genevieve Mattei</name>
<affiliations>
<json:string>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</json:string>
</affiliations>
</json:item>
<json:item>
<name>Marie-France Champliaud</name>
<affiliations>
<json:string>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</json:string>
</affiliations>
</json:item>
<json:item>
<name>Robert E. Burgeson</name>
<affiliations>
<json:string>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</json:string>
</affiliations>
</json:item>
<json:item>
<name>Reidar Albrechtsen</name>
<affiliations>
<json:string>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</json:string>
</affiliations>
</json:item>
</author>
<arkIstex>ark:/67375/6H6-SMK5QDD8-6</arkIstex>
<language>
<json:string>eng</json:string>
</language>
<originalGenre>
<json:string>Full-length article</json:string>
</originalGenre>
<abstract>Abstract: Overlapping cDNA clones that encode the full-length human laminin β2 chain, formerly called the S chain, were isolated. The cDNA of 5680 nt contains a 5391-nt open reading frame encoding 1797 amino acids. At the amino terminus is a 32-amino-acid signal peptide that is followed by the mature β2 chain polypeptide of 1765 amino acids with a calculated molecular mass of 192,389 Da. The human β2 chain is predicted to have all of the seven structural domains typical of the β chains of laminin, including the short cysteine-rich α region. The amino acid sequence of human β2 chain showed 86.1% sequence identity to the rat β2 chain, 50.0% to the human β1 chain, and 36.3% to the human β3 chain. The greatest sequence identity was in domains VI, V, and III. The sequence of a 24-amino-acid peptide fragment isolated from the β2 chain of laminin purified from human amniotic basement membrane matched the sequence predicted from the cDNA, confirming that the cDNA encodes human β2 laminin. The cDNA was used to assign the gene (LAMB2) to human chromosome 3p21 by in situ hybridization. It is not linked to genes for human laminin chains α1, β1, and γ1 or other known laminin genes. Immunostaining showed that the β2 chain is localized to the smooth muscle basement membranes of the arteries, while the homologous β1 chain is confined to the subendothelial basement membranes. The β2 chain was found in the basement membranes of ovarian carcinomas but not colon carcinomas. These results indicate that the expression of the β2 chain gene is tightly regulated in normal human tissues and in disease.</abstract>
<qualityIndicators>
<score>5.05</score>
<pdfWordCount>0</pdfWordCount>
<pdfCharCount>0</pdfCharCount>
<pdfVersion>1.2</pdfVersion>
<pdfPageCount>10</pdfPageCount>
<pdfPageSize>623 x 812 pts</pdfPageSize>
<refBibsNative>false</refBibsNative>
<abstractWordCount>269</abstractWordCount>
<abstractCharCount>1603</abstractCharCount>
<keywordCount>0</keywordCount>
</qualityIndicators>
<title>Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas</title>
<pmid>
<json:string>7698745</json:string>
</pmid>
<pii>
<json:string>S0888-7543(84)71612-0</json:string>
</pii>
<genre>
<json:string>research-article</json:string>
</genre>
<host>
<title>Genomics</title>
<language>
<json:string>unknown</json:string>
</language>
<publicationDate>1994</publicationDate>
<issn>
<json:string>0888-7543</json:string>
</issn>
<pii>
<json:string>S0888-7543(00)X0141-3</json:string>
</pii>
<volume>24</volume>
<issue>2</issue>
<pages>
<first>243</first>
<last>252</last>
</pages>
<genre>
<json:string>journal</json:string>
</genre>
</host>
<namedEntities>
<unitex>
<date></date>
<geogName></geogName>
<orgName></orgName>
<orgName_funder></orgName_funder>
<orgName_provider></orgName_provider>
<persName></persName>
<placeName></placeName>
<ref_url></ref_url>
<ref_bibl></ref_bibl>
<bibl></bibl>
</unitex>
</namedEntities>
<ark>
<json:string>ark:/67375/6H6-SMK5QDD8-6</json:string>
</ark>
<categories>
<wos>
<json:string>1 - science</json:string>
<json:string>2 - genetics & heredity</json:string>
<json:string>2 - biotechnology & applied microbiology</json:string>
</wos>
<scienceMetrix>
<json:string>1 - health sciences</json:string>
<json:string>2 - biomedical research</json:string>
<json:string>3 - genetics & heredity</json:string>
</scienceMetrix>
<scopus>
<json:string>1 - Life Sciences</json:string>
<json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string>
<json:string>3 - Genetics</json:string>
</scopus>
<inist>
<json:string>1 - sciences appliquees, technologies et medecines</json:string>
<json:string>2 - sciences biologiques et medicales</json:string>
<json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string>
</inist>
</categories>
<publicationDate>1994</publicationDate>
<copyrightDate>1994</copyrightDate>
<doi>
<json:string>10.1006/geno.1994.1612</json:string>
</doi>
<id>57DED64C05F928A8500CD1F4A2B650F1745C2FF5</id>
<score>1</score>
<fulltext>
<json:item>
<extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-SMK5QDD8-6/fulltext.pdf</uri>
</json:item>
<json:item>
<extension>ocr</extension>
<original>false</original>
<mimetype>text/ocr</mimetype>
<langDetect>
<reliable>false</reliable>
<languages>
<json:item>
<score>597</score>
<code>en</code>
<name>ENGLISH</name>
<percent>78</percent>
</json:item>
<json:item>
<score>1071</score>
<code>da</code>
<name>DANISH</name>
<percent>0</percent>
</json:item>
</languages>
</langDetect>
<uri>https://api.istex.fr/ark:/67375/6H6-SMK5QDD8-6/fulltext.ocr</uri>
</json:item>
<json:item>
<extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-SMK5QDD8-6/bundle.zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-SMK5QDD8-6/fulltext.tei">
<teiHeader>
<fileDesc>
<titleStmt>
<title level="a" type="main" xml:lang="en">Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas</title>
</titleStmt>
<publicationStmt>
<authority>ISTEX</authority>
<publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher>
<availability>
<licence>
<p>©1994 Academic Press</p>
</licence>
<p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p>
</availability>
<date>1994</date>
</publicationStmt>
<notesStmt>
<note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note>
<note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note>
<note type="content">Section title: Regular Article</note>
</notesStmt>
<sourceDesc>
<biblStruct type="inbook">
<analytic>
<title level="a" type="main" xml:lang="en">Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas</title>
<author xml:id="author-0000">
<persName>
<forename type="first">Ulla M.</forename>
<surname>Wewer</surname>
</persName>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
</author>
<author xml:id="author-0001">
<persName>
<forename type="first">Donald R.</forename>
<surname>Gerecke</surname>
</persName>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
</author>
<author xml:id="author-0002">
<persName>
<forename type="first">Marian E.</forename>
<surname>Durkin</surname>
</persName>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
</author>
<author xml:id="author-0003">
<persName>
<forename type="first">Kenneth S.</forename>
<surname>Kurtz</surname>
</persName>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
</author>
<author xml:id="author-0004">
<persName>
<forename type="first">Marie-Genevieve</forename>
<surname>Mattei</surname>
</persName>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
</author>
<author xml:id="author-0005">
<persName>
<forename type="first">Marie-France</forename>
<surname>Champliaud</surname>
</persName>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
</author>
<author xml:id="author-0006">
<persName>
<forename type="first">Robert E.</forename>
<surname>Burgeson</surname>
</persName>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
</author>
<author xml:id="author-0007">
<persName>
<forename type="first">Reidar</forename>
<surname>Albrechtsen</surname>
</persName>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
</author>
<idno type="istex">57DED64C05F928A8500CD1F4A2B650F1745C2FF5</idno>
<idno type="ark">ark:/67375/6H6-SMK5QDD8-6</idno>
<idno type="DOI">10.1006/geno.1994.1612</idno>
<idno type="PII">S0888-7543(84)71612-0</idno>
</analytic>
<monogr>
<title level="j">Genomics</title>
<title level="j" type="abbrev">YGENO</title>
<idno type="pISSN">0888-7543</idno>
<idno type="PII">S0888-7543(00)X0141-3</idno>
<imprint>
<publisher>ELSEVIER</publisher>
<date type="published" when="1994"></date>
<biblScope unit="volume">24</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="243">243</biblScope>
<biblScope unit="page" to="252">252</biblScope>
</imprint>
</monogr>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<creation>
<date>1994</date>
</creation>
<langUsage>
<language ident="en">en</language>
</langUsage>
<abstract xml:lang="en">
<p>Abstract: Overlapping cDNA clones that encode the full-length human laminin β2 chain, formerly called the S chain, were isolated. The cDNA of 5680 nt contains a 5391-nt open reading frame encoding 1797 amino acids. At the amino terminus is a 32-amino-acid signal peptide that is followed by the mature β2 chain polypeptide of 1765 amino acids with a calculated molecular mass of 192,389 Da. The human β2 chain is predicted to have all of the seven structural domains typical of the β chains of laminin, including the short cysteine-rich α region. The amino acid sequence of human β2 chain showed 86.1% sequence identity to the rat β2 chain, 50.0% to the human β1 chain, and 36.3% to the human β3 chain. The greatest sequence identity was in domains VI, V, and III. The sequence of a 24-amino-acid peptide fragment isolated from the β2 chain of laminin purified from human amniotic basement membrane matched the sequence predicted from the cDNA, confirming that the cDNA encodes human β2 laminin. The cDNA was used to assign the gene (LAMB2) to human chromosome 3p21 by in situ hybridization. It is not linked to genes for human laminin chains α1, β1, and γ1 or other known laminin genes. Immunostaining showed that the β2 chain is localized to the smooth muscle basement membranes of the arteries, while the homologous β1 chain is confined to the subendothelial basement membranes. The β2 chain was found in the basement membranes of ovarian carcinomas but not colon carcinomas. These results indicate that the expression of the β2 chain gene is tightly regulated in normal human tissues and in disease.</p>
</abstract>
</profileDesc>
<revisionDesc>
<change when="1994">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item>
<extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-SMK5QDD8-6/fulltext.txt</uri>
</json:item>
</fulltext>
<metadata>
<istex:metadataXml wicri:clean="Elsevier converted-article found">
<istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType>
<istex:document>
<converted-article version="4.5.2" docsubtype="fla" xml:lang="en">
<item-info>
<jid>YGENO</jid>
<aid>71612</aid>
<ce:pii>S0888-7543(84)71612-0</ce:pii>
<ce:doi>10.1006/geno.1994.1612</ce:doi>
<ce:copyright type="full-transfer" year="1994">Academic Press</ce:copyright>
</item-info>
<head>
<ce:dochead>
<ce:textfn>Regular Article</ce:textfn>
</ce:dochead>
<ce:title>Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas</ce:title>
<ce:author-group>
<ce:author>
<ce:given-name>Ulla M.</ce:given-name>
<ce:surname>Wewer</ce:surname>
</ce:author>
<ce:author>
<ce:given-name>Donald R.</ce:given-name>
<ce:surname>Gerecke</ce:surname>
</ce:author>
<ce:author>
<ce:given-name>Marian E.</ce:given-name>
<ce:surname>Durkin</ce:surname>
</ce:author>
<ce:author>
<ce:given-name>Kenneth S.</ce:given-name>
<ce:surname>Kurtz</ce:surname>
</ce:author>
<ce:author>
<ce:given-name>Marie-Genevieve</ce:given-name>
<ce:surname>Mattei</ce:surname>
</ce:author>
<ce:author>
<ce:given-name>Marie-France</ce:given-name>
<ce:surname>Champliaud</ce:surname>
</ce:author>
<ce:author>
<ce:given-name>Robert E.</ce:given-name>
<ce:surname>Burgeson</ce:surname>
</ce:author>
<ce:author>
<ce:given-name>Reidar</ce:given-name>
<ce:surname>Albrechtsen</ce:surname>
</ce:author>
<ce:affiliation>
<ce:textfn>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</ce:textfn>
</ce:affiliation>
</ce:author-group>
<ce:abstract>
<ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec>
<ce:simple-para>Overlapping cDNA clones that encode the full-length human laminin β2 chain, formerly called the S chain, were isolated. The cDNA of 5680 nt contains a 5391-nt open reading frame encoding 1797 amino acids. At the amino terminus is a 32-amino-acid signal peptide that is followed by the mature β2 chain polypeptide of 1765 amino acids with a calculated molecular mass of 192,389 Da. The human β2 chain is predicted to have all of the seven structural domains typical of the β chains of laminin, including the short cysteine-rich α region. The amino acid sequence of human β2 chain showed 86.1% sequence identity to the rat β2 chain, 50.0% to the human β1 chain, and 36.3% to the human β3 chain. The greatest sequence identity was in domains VI, V, and III. The sequence of a 24-amino-acid peptide fragment isolated from the β2 chain of laminin purified from human amniotic basement membrane matched the sequence predicted from the cDNA, confirming that the cDNA encodes human β2 laminin. The cDNA was used to assign the gene (LAMB2) to human chromosome 3p21 by
<ce:italic>in situ</ce:italic>
hybridization. It is not linked to genes for human laminin chains α1, β1, and γ1 or other known laminin genes. Immunostaining showed that the β2 chain is localized to the smooth muscle basement membranes of the arteries, while the homologous β1 chain is confined to the subendothelial basement membranes. The β2 chain was found in the basement membranes of ovarian carcinomas but not colon carcinomas. These results indicate that the expression of the β2 chain gene is tightly regulated in normal human tissues and in disease.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
</head>
</converted-article>
</istex:document>
</istex:metadataXml>
<mods version="3.6">
<titleInfo lang="en">
<title>Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas</title>
</titleInfo>
<titleInfo type="alternative" lang="en" contentType="CDATA">
<title>Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas</title>
</titleInfo>
<name type="personal">
<namePart type="given">Ulla M.</namePart>
<namePart type="family">Wewer</namePart>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Donald R.</namePart>
<namePart type="family">Gerecke</namePart>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Marian E.</namePart>
<namePart type="family">Durkin</namePart>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Kenneth S.</namePart>
<namePart type="family">Kurtz</namePart>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Marie-Genevieve</namePart>
<namePart type="family">Mattei</namePart>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Marie-France</namePart>
<namePart type="family">Champliaud</namePart>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Robert E.</namePart>
<namePart type="family">Burgeson</namePart>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal">
<namePart type="given">Reidar</namePart>
<namePart type="family">Albrechtsen</namePart>
<affiliation>The Laboratory of Molecular Pathology, University Institute of Pathological Anatomy, Copenhagen, Denmark; The Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts; and U242 INSERM, Hı̂pital d'Enfants de la Timone, Marseille 13385, France</affiliation>
<role>
<roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre>
<originInfo>
<publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1994</dateIssued>
<copyrightDate encoding="w3cdtf">1994</copyrightDate>
</originInfo>
<language>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
<languageTerm type="code" authority="rfc3066">en</languageTerm>
</language>
<abstract lang="en">Abstract: Overlapping cDNA clones that encode the full-length human laminin β2 chain, formerly called the S chain, were isolated. The cDNA of 5680 nt contains a 5391-nt open reading frame encoding 1797 amino acids. At the amino terminus is a 32-amino-acid signal peptide that is followed by the mature β2 chain polypeptide of 1765 amino acids with a calculated molecular mass of 192,389 Da. The human β2 chain is predicted to have all of the seven structural domains typical of the β chains of laminin, including the short cysteine-rich α region. The amino acid sequence of human β2 chain showed 86.1% sequence identity to the rat β2 chain, 50.0% to the human β1 chain, and 36.3% to the human β3 chain. The greatest sequence identity was in domains VI, V, and III. The sequence of a 24-amino-acid peptide fragment isolated from the β2 chain of laminin purified from human amniotic basement membrane matched the sequence predicted from the cDNA, confirming that the cDNA encodes human β2 laminin. The cDNA was used to assign the gene (LAMB2) to human chromosome 3p21 by in situ hybridization. It is not linked to genes for human laminin chains α1, β1, and γ1 or other known laminin genes. Immunostaining showed that the β2 chain is localized to the smooth muscle basement membranes of the arteries, while the homologous β1 chain is confined to the subendothelial basement membranes. The β2 chain was found in the basement membranes of ovarian carcinomas but not colon carcinomas. These results indicate that the expression of the β2 chain gene is tightly regulated in normal human tissues and in disease.</abstract>
<note type="content">Section title: Regular Article</note>
<relatedItem type="host">
<titleInfo>
<title>Genomics</title>
</titleInfo>
<titleInfo type="abbreviated">
<title>YGENO</title>
</titleInfo>
<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<originInfo>
<publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1994</dateIssued>
</originInfo>
<identifier type="ISSN">0888-7543</identifier>
<identifier type="PII">S0888-7543(00)X0141-3</identifier>
<part>
<date>1994</date>
<detail type="volume">
<number>24</number>
<caption>vol.</caption>
</detail>
<detail type="issue">
<number>2</number>
<caption>no.</caption>
</detail>
<extent unit="issue-pages">
<start>199</start>
<end>417</end>
</extent>
<extent unit="pages">
<start>243</start>
<end>252</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">57DED64C05F928A8500CD1F4A2B650F1745C2FF5</identifier>
<identifier type="ark">ark:/67375/6H6-SMK5QDD8-6</identifier>
<identifier type="DOI">10.1006/geno.1994.1612</identifier>
<identifier type="PII">S0888-7543(84)71612-0</identifier>
<accessCondition type="use and reproduction" contentType="copyright">©1994 Academic Press</accessCondition>
<recordInfo>
<recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource>
<recordOrigin>Academic Press, ©1994</recordOrigin>
</recordInfo>
</mods>
<json:item>
<extension>json</extension>
<original>false</original>
<mimetype>application/json</mimetype>
<uri>https://api.istex.fr/ark:/67375/6H6-SMK5QDD8-6/record.json</uri>
</json:item>
</metadata>
<serie></serie>
</istex>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002052 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 002052 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Istex
   |étape=   Corpus
   |type=    RBID
   |clé=     ISTEX:57DED64C05F928A8500CD1F4A2B650F1745C2FF5
   |texte=   Human β2 Chain of Laminin (Formerly S Chain): cDNA Cloning, Chromosomal Localization, and Expression in Carcinomas
}}

Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021