Effects of the gag Region on Genome Stability: Avian Retroviral Vectors That Contain Sequences from the Bryan Strain of Rous Sarcoma Virus
Identifieur interne : 002046 ( Istex/Corpus ); précédent : 002045; suivant : 002047Effects of the gag Region on Genome Stability: Avian Retroviral Vectors That Contain Sequences from the Bryan Strain of Rous Sarcoma Virus
Auteurs : Mark J. Federspiel ; Stephen H. HughesSource :
- Virology [ 0042-6822 ] ; 1994.
Abstract
Abstract: We have previously described replication-competent Schmidt Ruppin-A Rous sarcoma virus (RSV)-based retroviral vectors that can be used to deliver and express genes in avian cells. We have continued to modify the prototype vectors to develop a more versatile and efficient system. Substitution of the polymerase (pol) region from the Bryan high-titer RSV (BH-RSV) for the SR-A RSV pol region of these retroviral vectors causes these viruses to replicate more efficiently. We cloned the gag regions from two independent SH-RSV-transformed cell lines and tested whether substituting either of these gag regions would improve the replication and/or gene expression of the vectors. Chimeric vectors were constructed in which the gag region of the prototype vector (SR-A RSV) was replaced with the corresponding segment of BH-RSV gag in vectors that had either the original SR-A RSV pol or the BH-RSV pol region. All vectors contained the bacterial chloramphenicol acetyltransferase gene (CAT). The results indicate that different SR-A RSV and BH-RSV gag-pol chimeras can significantly affect the level of vital and CAT gene expression. The insertion of one of the BH-RSV gag regions, but not the other, gave rise to viruses with unstable genomes.
Url:
DOI: 10.1006/viro.1994.1478
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Federspiel</name><affiliation><mods:affiliation>ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, P.O. Box B, Frederick, Maryland 21702-1201</mods:affiliation></affiliation></author><author><name sortKey="Hughes, Stephen H" sort="Hughes, Stephen H" uniqKey="Hughes S" first="Stephen H." last="Hughes">Stephen H. Hughes</name><affiliation><mods:affiliation>ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, P.O. Box B, Frederick, Maryland 21702-1201</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">203</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="211">211</biblScope><biblScope unit="page" to="220">220</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have previously described replication-competent Schmidt Ruppin-A Rous sarcoma virus (RSV)-based retroviral vectors that can be used to deliver and express genes in avian cells. We have continued to modify the prototype vectors to develop a more versatile and efficient system. Substitution of the polymerase (pol) region from the Bryan high-titer RSV (BH-RSV) for the SR-A RSV pol region of these retroviral vectors causes these viruses to replicate more efficiently. We cloned the gag regions from two independent SH-RSV-transformed cell lines and tested whether substituting either of these gag regions would improve the replication and/or gene expression of the vectors. Chimeric vectors were constructed in which the gag region of the prototype vector (SR-A RSV) was replaced with the corresponding segment of BH-RSV gag in vectors that had either the original SR-A RSV pol or the BH-RSV pol region. All vectors contained the bacterial chloramphenicol acetyltransferase gene (CAT). The results indicate that different SR-A RSV and BH-RSV gag-pol chimeras can significantly affect the level of vital and CAT gene expression. The insertion of one of the BH-RSV gag regions, but not the other, gave rise to viruses with unstable genomes.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Mark J. Federspiel</name><affiliations><json:string>ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, P.O. Box B, Frederick, Maryland 21702-1201</json:string></affiliations></json:item><json:item><name>Stephen H. Hughes</name><affiliations><json:string>ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, P.O. 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We have continued to modify the prototype vectors to develop a more versatile and efficient system. Substitution of the polymerase (pol) region from the Bryan high-titer RSV (BH-RSV) for the SR-A RSV pol region of these retroviral vectors causes these viruses to replicate more efficiently. We cloned the gag regions from two independent SH-RSV-transformed cell lines and tested whether substituting either of these gag regions would improve the replication and/or gene expression of the vectors. Chimeric vectors were constructed in which the gag region of the prototype vector (SR-A RSV) was replaced with the corresponding segment of BH-RSV gag in vectors that had either the original SR-A RSV pol or the BH-RSV pol region. All vectors contained the bacterial chloramphenicol acetyltransferase gene (CAT). The results indicate that different SR-A RSV and BH-RSV gag-pol chimeras can significantly affect the level of vital and CAT gene expression. 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Box B, Frederick, Maryland 21702-1201</ce:textfn></ce:affiliation></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>We have previously described replication-competent Schmidt Ruppin-A Rous sarcoma virus (RSV)-based retroviral vectors that can be used to deliver and express genes in avian cells. We have continued to modify the prototype vectors to develop a more versatile and efficient system. Substitution of the polymerase (<ce:italic>pol</ce:italic>) region from the Bryan high-titer RSV (BH-RSV) for the SR-A RSV <ce:italic>pol</ce:italic> region of these retroviral vectors causes these viruses to replicate more efficiently. We cloned the <ce:italic>gag</ce:italic> regions from two independent SH-RSV-transformed cell lines and tested whether substituting either of these <ce:italic>gag</ce:italic> regions would improve the replication and/or gene expression of the vectors. Chimeric vectors were constructed in which the <ce:italic>gag</ce:italic> region of the prototype vector (SR-A RSV) was replaced with the corresponding segment of BH-RSV <ce:italic>gag</ce:italic> in vectors that had either the original SR-A RSV <ce:italic>pol</ce:italic> or the BH-RSV <ce:italic>pol</ce:italic> region. All vectors contained the bacterial chloramphenicol acetyltransferase gene (CAT). The results indicate that different SR-A RSV and BH-RSV <ce:italic>gag-pol</ce:italic> chimeras can significantly affect the level of vital and CAT gene expression. The insertion of one of the BH-RSV <ce:italic>gag</ce:italic> regions, but not the other, gave rise to viruses with unstable genomes.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Effects of the gag Region on Genome Stability: Avian Retroviral Vectors That Contain Sequences from the Bryan Strain of Rous Sarcoma Virus</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Effects of the</title></titleInfo><name type="personal"><namePart type="given">Mark J.</namePart><namePart type="family">Federspiel</namePart><affiliation>ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, P.O. Box B, Frederick, Maryland 21702-1201</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephen H.</namePart><namePart type="family">Hughes</namePart><affiliation>ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, P.O. 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Substitution of the polymerase (pol) region from the Bryan high-titer RSV (BH-RSV) for the SR-A RSV pol region of these retroviral vectors causes these viruses to replicate more efficiently. We cloned the gag regions from two independent SH-RSV-transformed cell lines and tested whether substituting either of these gag regions would improve the replication and/or gene expression of the vectors. Chimeric vectors were constructed in which the gag region of the prototype vector (SR-A RSV) was replaced with the corresponding segment of BH-RSV gag in vectors that had either the original SR-A RSV pol or the BH-RSV pol region. All vectors contained the bacterial chloramphenicol acetyltransferase gene (CAT). The results indicate that different SR-A RSV and BH-RSV gag-pol chimeras can significantly affect the level of vital and CAT gene expression. 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