MersV1, Istex, Corpus, bibRecord, 001F57

Functional Mapping of Regions of the Autographa californica Nuclear Polyhedrosis Viral Genome Required for DNA Replication

Identifieur interne : 001F57 ( Istex/Corpus ); précédent : 001F56; suivant : 001F58

Functional Mapping of Regions of the Autographa californica Nuclear Polyhedrosis Viral Genome Required for DNA Replication

Auteurs : M. Kool ; J. T. M. Voeten ; R. W. Goldbach ; J. M. Vlak

Source :

Virology [ 0042-6822 ] ; 1994.

RBID : ISTEX:75D971650B857553179090625C29DCA82FD9060F

Abstract

Abstract: Previous results showed that plasmids containing one of the eight putative origins (ori's) of Autographa californica nuclear polyhedrosis virus (AcMNPV) are replicated after transfection into Spodoptera frugiperda cells if essential trans-acting factors are supplied by AcMNPV infection (Kool et al., Virology, 192, 94-101, 1993a; Kool et al., J. Gen. Virol., in press, 1993b; Leisy and Rohrmann, Virology, 196, 722-730, 1993). In this report a transient complementation assay is described in which four cotransfected cosmid clones, instead of AcMNPV infection, provided essential transacting factors for plasmid DNA replication. In this assay plasmid replication was found to be independent of the presence, in cis, of a viral ori. No replication of plasmids occurred when one of the cosmids was omitted from the transfection mixture. This result indicated that this assay is a valid approach for identification of AcMNPV replication genes. We further used the assay to define essential regions in the four required cosmids. Six regions of the AcMNPV genome, Eco RI-I (map unit 0.3-5.8), EcoRI-O (map unit 6.9-8.7), Sst I-F (map unit 38.9-45.0), EcoRI-D (map unit 59.968.3), a Bam HI-SstII fragment of BamHI-B (map unit 84.3-89.7), and Eco RI-B (map unit 90.0-100), with at least seven genes, were found to be essential for plasmid DNA replication. These regions contain the putative DNA polymerase gene (SstI-F), the helicase-like gene (EcoRI-D), and the region where most of the trans-activating immediate-early genes of AcMNPV are located (EcoRI-B). For SstI-F it was shown that this region contains besides the DNA polymerase gene at least one other replication gene. These results show that it will now be possible to define the set of AcMNPV genes necessary and sufficient for DNA replication.

Url:

https://api.istex.fr/ark:/67375/6H6-D5TTFR23-F/fulltext.pdf

DOI: 10.1006/viro.1994.1080

Links to Exploration step

ISTEX:75D971650B857553179090625C29DCA82FD9060F

Le document en format XML

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<abstract xml:lang="en"><p>Abstract: Previous results showed that plasmids containing one of the eight putative origins (ori's) of Autographa californica nuclear polyhedrosis virus (AcMNPV) are replicated after transfection into Spodoptera frugiperda cells if essential trans-acting factors are supplied by AcMNPV infection (Kool et al., Virology, 192, 94-101, 1993a; Kool et al., J. Gen. Virol., in press, 1993b; Leisy and Rohrmann, Virology, 196, 722-730, 1993). In this report a transient complementation assay is described in which four cotransfected cosmid clones, instead of AcMNPV infection, provided essential transacting factors for plasmid DNA replication. In this assay plasmid replication was found to be independent of the presence, in cis, of a viral ori. No replication of plasmids occurred when one of the cosmids was omitted from the transfection mixture. This result indicated that this assay is a valid approach for identification of AcMNPV replication genes. We further used the assay to define essential regions in the four required cosmids. Six regions of the AcMNPV genome, Eco RI-I (map unit 0.3-5.8), EcoRI-O (map unit 6.9-8.7), Sst I-F (map unit 38.9-45.0), EcoRI-D (map unit 59.968.3), a Bam HI-SstII fragment of BamHI-B (map unit 84.3-89.7), and Eco RI-B (map unit 90.0-100), with at least seven genes, were found to be essential for plasmid DNA replication. These regions contain the putative DNA polymerase gene (SstI-F), the helicase-like gene (EcoRI-D), and the region where most of the trans-activating immediate-early genes of AcMNPV are located (EcoRI-B). For SstI-F it was shown that this region contains besides the DNA polymerase gene at least one other replication gene. These results show that it will now be possible to define the set of AcMNPV genes necessary and sufficient for DNA replication.</p>
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<ce:title>Functional Mapping of Regions of the <ce:italic>Autographa californica</ce:italic>
 Nuclear Polyhedrosis Viral Genome Required for DNA Replication</ce:title>
<ce:author-group><ce:author><ce:given-name>M.</ce:given-name>
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<ce:abstract><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para>Previous results showed that plasmids containing one of the eight putative origins (<ce:italic>ori's</ce:italic>
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 nuclear polyhedrosis virus (Ac<ce:italic>M</ce:italic>
NPV) are replicated after transfection into <ce:italic>Spodoptera frugiperda</ce:italic>
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-acting factors are supplied by Ac<ce:italic>M</ce:italic>
NPV infection (Kool et al., Virology, 192, 94-101, 1993a; Kool <ce:italic>et al., J. Gen. Virol.,</ce:italic>
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NPV infection, provided essential transacting factors for plasmid DNA replication. In this assay plasmid replication was found to be independent of the presence, in <ce:italic>cis,</ce:italic>
 of a viral <ce:italic>ori.</ce:italic>
 No replication of plasmids occurred when one of the cosmids was omitted from the transfection mixture. This result indicated that this assay is a valid approach for identification of Ac<ce:italic>M</ce:italic>
NPV replication genes. We further used the assay to define essential regions in the four required cosmids. Six regions of the Ac<ce:italic>M</ce:italic>
NPV genome, <ce:italic>Eco</ce:italic>
 RI-I (map unit 0.3-5.8), <ce:italic>Eco</ce:italic>
RI-O (map unit 6.9-8.7), <ce:italic>Sst</ce:italic>
 I-F (map unit 38.9-45.0), <ce:italic>Eco</ce:italic>
RI-D (map unit 59.968.3), a <ce:italic>Bam</ce:italic>
 HI-<ce:italic>Sst</ce:italic>
II fragment of <ce:italic>Bam</ce:italic>
HI-B (map unit 84.3-89.7), and <ce:italic>Eco</ce:italic>
 RI-B (map unit 90.0-100), with at least seven genes, were found to be essential for plasmid DNA replication. These regions contain the putative DNA polymerase gene (<ce:italic>Sst</ce:italic>
I-F), the helicase-like gene (<ce:italic>Eco</ce:italic>
RI-D), and the region where most of the <ce:italic>trans</ce:italic>
-activating immediate-early genes of Ac<ce:italic>M</ce:italic>
NPV are located (<ce:italic>Eco</ce:italic>
RI-B). For <ce:italic>Sst</ce:italic>
I-F it was shown that this region contains besides the DNA polymerase gene at least one other replication gene. These results show that it will now be possible to define the set of Ac<ce:italic>M</ce:italic>
NPV genes necessary and sufficient for DNA replication.</ce:simple-para>
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<mods version="3.6"><titleInfo lang="en"><title>Functional Mapping of Regions of the Autographa californica Nuclear Polyhedrosis Viral Genome Required for DNA Replication</title>
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<titleInfo type="alternative" lang="en" contentType="CDATA"><title>Functional Mapping of Regions of the</title>
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<name type="personal"><namePart type="given">M.</namePart>
<namePart type="family">Kool</namePart>
<affiliation>Department of Virology, Wageningen Agricultural University, P.O. Box 8045, 6700 EM Wageningen, The Netherlands</affiliation>
<role><roleTerm type="text">author</roleTerm>
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</name>
<name type="personal"><namePart type="given">J.T.M.</namePart>
<namePart type="family">Voeten</namePart>
<affiliation>Department of Virology, Wageningen Agricultural University, P.O. Box 8045, 6700 EM Wageningen, The Netherlands</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">R.W.</namePart>
<namePart type="family">Goldbach</namePart>
<affiliation>Department of Virology, Wageningen Agricultural University, P.O. Box 8045, 6700 EM Wageningen, The Netherlands</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">J.M.</namePart>
<namePart type="family">Vlak</namePart>
<affiliation>Department of Virology, Wageningen Agricultural University, P.O. Box 8045, 6700 EM Wageningen, The Netherlands</affiliation>
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<dateIssued encoding="w3cdtf">1994</dateIssued>
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<abstract lang="en">Abstract: Previous results showed that plasmids containing one of the eight putative origins (ori's) of Autographa californica nuclear polyhedrosis virus (AcMNPV) are replicated after transfection into Spodoptera frugiperda cells if essential trans-acting factors are supplied by AcMNPV infection (Kool et al., Virology, 192, 94-101, 1993a; Kool et al., J. Gen. Virol., in press, 1993b; Leisy and Rohrmann, Virology, 196, 722-730, 1993). In this report a transient complementation assay is described in which four cotransfected cosmid clones, instead of AcMNPV infection, provided essential transacting factors for plasmid DNA replication. In this assay plasmid replication was found to be independent of the presence, in cis, of a viral ori. No replication of plasmids occurred when one of the cosmids was omitted from the transfection mixture. This result indicated that this assay is a valid approach for identification of AcMNPV replication genes. We further used the assay to define essential regions in the four required cosmids. Six regions of the AcMNPV genome, Eco RI-I (map unit 0.3-5.8), EcoRI-O (map unit 6.9-8.7), Sst I-F (map unit 38.9-45.0), EcoRI-D (map unit 59.968.3), a Bam HI-SstII fragment of BamHI-B (map unit 84.3-89.7), and Eco RI-B (map unit 90.0-100), with at least seven genes, were found to be essential for plasmid DNA replication. These regions contain the putative DNA polymerase gene (SstI-F), the helicase-like gene (EcoRI-D), and the region where most of the trans-activating immediate-early genes of AcMNPV are located (EcoRI-B). For SstI-F it was shown that this region contains besides the DNA polymerase gene at least one other replication gene. These results show that it will now be possible to define the set of AcMNPV genes necessary and sufficient for DNA replication.</abstract>
<note type="content">Section title: Regular Article</note>
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<dateIssued encoding="w3cdtf">1994</dateIssued>
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<identifier type="ISSN">0042-6822</identifier>
<identifier type="PII">S0042-6822(00)X0172-9</identifier>
<part><date>1994</date>
<detail type="volume"><number>198</number>
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<extent unit="issue-pages"><start>415</start>
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<identifier type="DOI">10.1006/viro.1994.1080</identifier>
<identifier type="PII">S0042-6822(84)71080-4</identifier>
<accessCondition type="use and reproduction" contentType="copyright">©1994 Academic Press</accessCondition>
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Functional Mapping of Regions of the Autographa californica Nuclear Polyhedrosis Viral Genome Required for DNA Replication

Functional Mapping of Regions of the Autographa californica Nuclear Polyhedrosis Viral Genome Required for DNA Replication

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