Extracellular-matrix gene expression during mouse submandibular gland development
Identifieur interne : 001F15 ( Istex/Corpus ); précédent : 001F14; suivant : 001F16Extracellular-matrix gene expression during mouse submandibular gland development
Auteurs : Shawn P. Macauley ; Roy W. Tarnuzzer ; Gregory S. Schultz ; Nasser Chegini ; Gregory E. Oxford ; Michael G. HumphreysbeherSource :
- Archives of Oral Biology [ 0003-9969 ] ; 1997.
English descriptors
- KwdEn :
- Teeft :
- Band intensities, Basal, Basal lamina, Basement membrane, Basement membranes, Biol, Cleft formation, Collagen, Collagen types, Ductal cells, Epithelial, Epithelial lobules, Epithelium, Extracellular, Extracellular matrix, Extracellular matrix components, Fetal, Fetal glands, Fetal submandibular glands, Fibronectin, Gene expression, Gland, Grobstein, Immunoreactive, Integrin receptor, Laminin, Lysyl, Lysyl oxidase, Macauley, Matrix, Mesenchymal, Mesenchymal capsule, Mesenchyme, Morphogenesis, Mouse submandibular gland, Mouse submandibular glands, Mrna, Nakanishi, Neonatal, Oligonucleotide, Oligonucleotide primers, Oral biology, Other days, Oxidase, Parotid, Parotid gland, Primary antibody, Primer, Receptor, Salivary, Salivary glands, Spooner, Striated ducts, Submandibular, Submandibular gland, Submandibular glands, Transcription reaction.
Abstract
Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.
Url:
DOI: 10.1016/S0003-9969(97)00027-7
Links to Exploration step
ISTEX:8F9CD54D0D5B213E55E24A6109D79DA6370829DFLe document en format XML
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Macauley">Shawn P. Macauley</name><affiliation><mods:affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>*Present address: Department of Genetics and Cell Biology, Health Research and Education Center, Washington State University at Spokane, Spokane, WA 99204- 0399, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="W Tarnuzzer, Roy" sort="W Tarnuzzer, Roy" uniqKey="W Tarnuzzer R" first="Roy" last="W. Tarnuzzer">Roy W. Tarnuzzer</name><affiliation><mods:affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="S Schultz, Gregory" sort="S Schultz, Gregory" uniqKey="S Schultz G" first="Gregory" last="S. Schultz">Gregory S. 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Humphreysbeher</name><affiliation><mods:affiliation>To whom all correspondence should be addressed, at the Department of Oral Biology.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Center for Orphan Diseases, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Oral Biology</title><title level="j" type="abbrev">AOB</title><idno type="ISSN">0003-9969</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">42</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="443">443</biblScope><biblScope unit="page" to="454">454</biblScope></imprint><idno type="ISSN">0003-9969</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-9969</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DEPC</term><term>RT-PCR</term><term>dNTP, rNTP</term><term>dT</term><term>extracellular matrix</term><term>mouse submandibular gland development</term><term>quantitative RT-PCR</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Band intensities</term><term>Basal</term><term>Basal lamina</term><term>Basement membrane</term><term>Basement membranes</term><term>Biol</term><term>Cleft formation</term><term>Collagen</term><term>Collagen types</term><term>Ductal cells</term><term>Epithelial</term><term>Epithelial lobules</term><term>Epithelium</term><term>Extracellular</term><term>Extracellular matrix</term><term>Extracellular matrix components</term><term>Fetal</term><term>Fetal glands</term><term>Fetal submandibular glands</term><term>Fibronectin</term><term>Gene expression</term><term>Gland</term><term>Grobstein</term><term>Immunoreactive</term><term>Integrin receptor</term><term>Laminin</term><term>Lysyl</term><term>Lysyl oxidase</term><term>Macauley</term><term>Matrix</term><term>Mesenchymal</term><term>Mesenchymal capsule</term><term>Mesenchyme</term><term>Morphogenesis</term><term>Mouse submandibular gland</term><term>Mouse submandibular glands</term><term>Mrna</term><term>Nakanishi</term><term>Neonatal</term><term>Oligonucleotide</term><term>Oligonucleotide primers</term><term>Oral biology</term><term>Other days</term><term>Oxidase</term><term>Parotid</term><term>Parotid gland</term><term>Primary antibody</term><term>Primer</term><term>Receptor</term><term>Salivary</term><term>Salivary glands</term><term>Spooner</term><term>Striated ducts</term><term>Submandibular</term><term>Submandibular gland</term><term>Submandibular glands</term><term>Transcription reaction</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>laminin</json:string><json:string>submandibular</json:string><json:string>fetal</json:string><json:string>morphogenesis</json:string><json:string>mrna</json:string><json:string>fibronectin</json:string><json:string>epithelial</json:string><json:string>spooner</json:string><json:string>extracellular</json:string><json:string>mesenchyme</json:string><json:string>primer</json:string><json:string>biol</json:string><json:string>lysyl</json:string><json:string>oxidase</json:string><json:string>nakanishi</json:string><json:string>collagen</json:string><json:string>epithelium</json:string><json:string>gene 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of Florida College of Medicine</json:string><json:string>Department of Oral Biology</json:string><json:string>Vector Laboratories</json:string><json:string>Invitrogen</json:string><json:string>Upstate Biotechnology</json:string><json:string>Chemicon International</json:string><json:string>Health Research and Education Center, Washington State University</json:string><json:string>Boston University</json:string><json:string>Department of Periodontology and Oral Biology</json:string><json:string>Department of Genetics and Cell Biology</json:string><json:string>University of Florida</json:string><json:string>NIH grant DE 08778</json:string></orgName><orgName_funder><json:string>NIH grant DE 08778</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Philip Trackman</json:string><json:string>Spooner</json:string><json:string>Mr Micah</json:string><json:string>Apple Macintosh</json:string><json:string>In</json:string><json:string>S. P. Macauley</json:string><json:string>Facility</json:string><json:string>Hardman</json:string><json:string>Committee</json:string><json:string>Drs L. Brinkley</json:string><json:string>J. Morris-Wyman</json:string><json:string>Nakanishi</json:string></persName><placeName><json:string>Birmingham</json:string><json:string>San Diego</json:string><json:string>Spokane</json:string><json:string>IN</json:string><json:string>Boston</json:string><json:string>Temecula</json:string><json:string>CA</json:string><json:string>MA</json:string><json:string>AL</json:string><json:string>FL</json:string><json:string>WA</json:string><json:string>Indianapolis</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Ausubel, 1987</json:string><json:string>S. P. Macauley et al.</json:string><json:string>Kratchowil et al., 1986</json:string><json:string>Trelstad, 1984</json:string><json:string>Nakanishi et al. (1988)</json:string><json:string>Grobstein and Cohen, 1965</json:string><json:string>Hoffman and Kleinman, 1996</json:string><json:string>Rugh, 1968</json:string><json:string>Bradhorst and McConkey, 1974</json:string><json:string>Lazowski et al. (1994)</json:string><json:string>S.P. Macauley et al.</json:string><json:string>Nakanishi et al., 1986</json:string><json:string>Spooner et al., 1985</json:string><json:string>Braga et al., 1992</json:string><json:string>Spooner et al. (1986)</json:string><json:string>Kidd et al., 1991</json:string><json:string>Chirgwin et al. (1979)</json:string><json:string>Redman (1987)</json:string><json:string>Cutler, 1990</json:string><json:string>Tarnuzzer et al., 1996</json:string><json:string>Cooper and MacQueen, 1983</json:string><json:string>Spooner and Faubion, 1980</json:string><json:string>Haqqi et al. (1992)</json:string><json:string>Nakanishi et al., 1986, 1988</json:string><json:string>Cutler, 1989</json:string><json:string>Fitch and Linsemayer, 1994</json:string><json:string>Siebert, 1993</json:string><json:string>Nogawa and Mizuno (1981)</json:string><json:string>Grobstein 1953a,b,c</json:string><json:string>Siebert and Larrick, 1992</json:string><json:string>Nakanishi and Ishii, 1989</json:string><json:string>Thompson and Spooner, 1982, 1983</json:string><json:string>Lazowski et al., 1994</json:string><json:string>Bernfield, 1984</json:string><json:string>Hardman and Spooner (1992)</json:string><json:string>Tarnuzzer et al., (1996)</json:string><json:string>Leivo et al., 1980</json:string><json:string>Redman, 1987</json:string><json:string>Raeymaekers, 1994, 1995</json:string><json:string>Begovac et al., 1994</json:string><json:string>Poulsen et al., 1986</json:string><json:string>Fukuda et al., 1988</json:string><json:string>NCSS; Hintze, 1995</json:string><json:string>Chegini et al. (1993)</json:string><json:string>Lakowski et al., 1994</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-Q9SG87X5-F</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - dentistry, oral surgery & medicine</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - dentistry</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Cell Biology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Dentistry</json:string><json:string>3 - General Dentistry</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Otorhinolaryngology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1997</publicationDate><author><json:item><name>Shawn P. Macauley</name><affiliations><json:string>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string><json:string>*Present address: Department of Genetics and Cell Biology, Health Research and Education Center, Washington State University at Spokane, Spokane, WA 99204- 0399, U.S.A.</json:string></affiliations></json:item><json:item><name>Roy W. Tarnuzzer</name><affiliations><json:string>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string><json:string>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string></affiliations></json:item><json:item><name>Gregory S. Schultz</name><affiliations><json:string>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string><json:string>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string></affiliations></json:item><json:item><name>Nasser Chegini</name><affiliations><json:string>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string><json:string>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string></affiliations></json:item><json:item><name>Gregory E. Oxford</name><affiliations><json:string>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string></affiliations></json:item><json:item><name>Michael G. Humphreysbeher</name><affiliations><json:string>To whom all correspondence should be addressed, at the Department of Oral Biology.</json:string><json:string>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string><json:string>Center for Orphan Diseases, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</json:string></affiliations></json:item></author><articleId><json:string>97000277</json:string></articleId><arkIstex>ark:/67375/6H6-Q9SG87X5-F</arkIstex><abstract>Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</abstract><pmid><json:string>9382709</json:string></pmid><serie><title>Handbook of Physiology: Salivary, Pancreatic, Gastric, and Hepatobilliary Secretion</title><language><json:string>unknown</json:string></language><volume>Vol. 3.</volume><pages><first>413</first><last>420</last></pages></serie><copyrightDate>1997</copyrightDate><doi><json:string>10.1016/S0003-9969(97)00027-7</json:string></doi><id>8F9CD54D0D5B213E55E24A6109D79DA6370829DF</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Q9SG87X5-F/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Q9SG87X5-F/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-Q9SG87X5-F/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Extracellular-matrix gene expression during mouse submandibular gland development</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1997</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Extracellular-matrix gene expression during mouse submandibular gland development</title><author xml:id="author-0000"><persName><forename type="first">Shawn</forename><surname>P. Macauley</surname></persName><affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>*Present address: Department of Genetics and Cell Biology, Health Research and Education Center, Washington State University at Spokane, Spokane, WA 99204- 0399, U.S.A.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Roy</forename><surname>W. Tarnuzzer</surname></persName><affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Gregory</forename><surname>S. Schultz</surname></persName><affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Nasser</forename><surname>Chegini</surname></persName><affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Gregory</forename><surname>E. Oxford</surname></persName><affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Michael</forename><surname>G. Humphreysbeher</surname></persName><affiliation>To whom all correspondence should be addressed, at the Department of Oral Biology.</affiliation><affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>Center for Orphan Diseases, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation></author><idno type="istex">8F9CD54D0D5B213E55E24A6109D79DA6370829DF</idno><idno type="ark">ark:/67375/6H6-Q9SG87X5-F</idno><idno type="DOI">10.1016/S0003-9969(97)00027-7</idno><idno type="PII">S0003-9969(97)00027-7</idno><idno type="article-id">97000277</idno></analytic><monogr><title level="j">Archives of Oral Biology</title><title level="j" type="abbrev">AOB</title><idno type="pISSN">0003-9969</idno><idno type="PII">S0003-9969(00)X0027-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997"></date><biblScope unit="volume">42</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="443">443</biblScope><biblScope unit="page" to="454">454</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1997</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>mouse submandibular gland development</term></item><item><term>quantitative RT-PCR</term></item><item><term>extracellular matrix</term></item></list></keywords></textClass><textClass xml:lang="en"><keywords scheme="keyword"><list><item><term>DEPC</term><term>diethyl pyrocarbonate</term></item><item><term>dT</term><term>deoxythymidine</term></item><item><term>dNTP, rNTP</term><term>deoxy- (and ribo-)nucleoside triphosphate</term></item><item><term>RT-PCR</term><term>reverse transcription-polymerase chain reaction</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1997">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Q9SG87X5-F/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" </istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.1.0//EN//XML" URI="art510.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.1" xml:lang="en" docsubtype="fla"><item-info><jid>AOB</jid><aid>97000277</aid><ce:pii>S0003-9969(97)00027-7</ce:pii><ce:doi>10.1016/S0003-9969(97)00027-7</ce:doi><ce:copyright type="unknown" year="1997"></ce:copyright></item-info><head><ce:title>Extracellular-matrix gene expression during mouse submandibular gland development</ce:title><ce:author-group><ce:author><ce:given-name>Shawn</ce:given-name><ce:surname>P. Macauley</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="fn1"><ce:sup>*</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Roy</ce:given-name><ce:surname>W. Tarnuzzer</ce:surname><ce:cross-ref refid="aff2"><ce:sup>2</ce:sup></ce:cross-ref><ce:cross-ref refid="aff3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Gregory</ce:given-name><ce:surname>S. Schultz</ce:surname><ce:cross-ref refid="aff2"><ce:sup>2</ce:sup></ce:cross-ref><ce:cross-ref refid="aff3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Nasser</ce:given-name><ce:surname>Chegini</ce:surname><ce:cross-ref refid="aff2"><ce:sup>2</ce:sup></ce:cross-ref><ce:cross-ref refid="aff3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Gregory</ce:given-name><ce:surname>E. Oxford</ce:surname><ce:cross-ref refid="aff1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Michael</ce:given-name><ce:surname>G. Humphreysbeher</ce:surname><ce:cross-ref refid="cor1"><ce:sup>†</ce:sup></ce:cross-ref><ce:cross-ref refid="aff1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="aff4"><ce:sup>4</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:label>a</ce:label><ce:textfn>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="aff2"><ce:label>b</ce:label><ce:textfn>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="aff3"><ce:label>c</ce:label><ce:textfn>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="aff4"><ce:label>d</ce:label><ce:textfn>Center for Orphan Diseases, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</ce:textfn></ce:affiliation><ce:correspondence id="cor1"><ce:label>†</ce:label><ce:text>To whom all correspondence should be addressed, at the Department of Oral Biology.</ce:text></ce:correspondence><ce:footnote id="fn1"><ce:label>*</ce:label><ce:note-para>Present address: Department of Genetics and Cell Biology, Health Research and Education Center, Washington State University at Spokane, Spokane, WA 99204- 0399, U.S.A.</ce:note-para></ce:footnote></ce:author-group><ce:date-accepted day="17" month="2" year="1997"></ce:date-accepted><ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para id="SP0005">Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword" xml:lang="en"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>mouse submandibular gland development</ce:text></ce:keyword><ce:keyword><ce:text>quantitative RT-PCR</ce:text></ce:keyword><ce:keyword><ce:text>extracellular matrix</ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:keyword><ce:text>DEPC</ce:text><ce:keyword><ce:text>diethyl pyrocarbonate</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>dT</ce:text><ce:keyword><ce:text>deoxythymidine</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>dNTP, rNTP</ce:text><ce:keyword><ce:text>deoxy- (and ribo-)nucleoside triphosphate</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>RT-PCR</ce:text><ce:keyword><ce:text>reverse transcription-polymerase chain reaction</ce:text></ce:keyword></ce:keyword></ce:keywords></head><tail><ce:bibliography id="R0005"><ce:section-title>Reference</ce:section-title><ce:bibliography-sec id="RS0005"><ce:bib-reference id="bib1"><ce:label>Ausubel, 1987</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Ausubel</ce:surname><ce:given-name>F.M.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>In situ polymerase chain reaction and hybridization to detect low-abundance nucleid acid targets</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:editors><sb:editor><ce:surname>Ausubel</ce:surname><ce:given-name>F.M.</ce:given-name></sb:editor></sb:editors><sb:title><sb:maintitle>Current Protocols in Molecular Biology</sb:maintitle></sb:title><sb:date>1987</sb:date><sb:publisher><sb:name>Greene Publishing Associates and Wiley-Interscience</sb:name><sb:location>New York</sb:location></sb:publisher></sb:edited-book></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib2"><ce:label>Begovac et al., 1994</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Begovac</ce:surname><ce:given-name>P.C.</ce:given-name></sb:author><sb:author><ce:surname>Shi</ce:surname><ce:given-name>Y.S.</ce:given-name></sb:author><sb:author><ce:surname>Mansfield</ce:surname><ce:given-name>D.</ce:given-name></sb:author><sb:author><ce:surname>Shur</ce:surname><ce:given-name>B.D.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Evidence that cell surface β<ce:inf>1,4</ce:inf>-galactosyltrans-ferase spontaneously galactosylates an underlying lami-nin substrate during fibroblast migration</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Biol. Chem.</sb:maintitle></sb:title><sb:volume-nr>269</sb:volume-nr></sb:series><sb:date>1994</sb:date></sb:issue><sb:pages><sb:first-page>31793</sb:first-page><sb:last-page>31799</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib3"><ce:label>Bernfield et al., 1984</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Bernfield</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Banerjee</ce:surname><ce:given-name>S.D.</ce:given-name></sb:author><sb:author><ce:surname>Koda</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Raprager</ce:surname><ce:given-name>A.C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Remodeling of the basement membrane: morphogenesis and maturation</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:editors><sb:editor><ce:surname>Porter</ce:surname><ce:given-name>R.</ce:given-name></sb:editor><sb:editor><ce:surname>Whelan</ce:surname><ce:given-name>J.</ce:given-name></sb:editor></sb:editors><sb:title><sb:maintitle>Basement Membranes and Cell Movement</sb:maintitle></sb:title><sb:date>1984</sb:date><sb:publisher><sb:name>Pittman Press</sb:name><sb:location>Bath</sb:location></sb:publisher></sb:edited-book><sb:pages><sb:first-page>179</sb:first-page><sb:last-page>188</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib4"><ce:label>Bernfield and Wessells, 1970</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Bernfield</ce:surname><ce:given-name>M.R.</ce:given-name></sb:author><sb:author><ce:surname>Wessells</ce:surname><ce:given-name>N.K.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Intra- and extracellular control of epithelial morphogenesis</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Dev. Biol.</sb:maintitle></sb:title><sb:volume-nr>4</sb:volume-nr></sb:series><sb:date>1970</sb:date></sb:issue><sb:pages><sb:first-page>195</sb:first-page><sb:last-page>249</sb:last-page></sb:pages></sb:host><sb:comment>Suppl</sb:comment></sb:reference></ce:bib-reference><ce:bib-reference id="bib5"><ce:label>Borghese, 1950</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Borghese</ce:surname><ce:given-name>E.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Explantation experiments on the influence of the connective tissue capsule on the development of the epithelial part of the submandibular gland of Mus musculus</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Anat. (London)</sb:maintitle></sb:title><sb:volume-nr>84</sb:volume-nr></sb:series><sb:date>1950</sb:date></sb:issue><sb:pages><sb:first-page>303</sb:first-page><sb:last-page>321</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib6"><ce:label>Bradhorst and McConkey, 1974</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Bradhorst</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>McConkey</ce:surname><ce:given-name>E.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Stability of nuclear RNA in mammalian cells</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Mol. Biol.</sb:maintitle></sb:title><sb:volume-nr>85</sb:volume-nr></sb:series><sb:date>1974</sb:date></sb:issue><sb:pages><sb:first-page>451</sb:first-page><sb:last-page>463</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib7"><ce:label>Braga et al., 1992</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Braga</ce:surname><ce:given-name>V.M.M.</ce:given-name></sb:author><sb:author><ce:surname>Pemberton</ce:surname><ce:given-name>L.F.</ce:given-name></sb:author><sb:author><ce:surname>Duhig</ce:surname><ce:given-name>T.</ce:given-name></sb:author><sb:author><ce:surname>Gendler</ce:surname><ce:given-name>S.J.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Spatial and temporal expression of an epithelial mucin, Muc-1, during mouse development</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Development</sb:maintitle></sb:title><sb:volume-nr>115</sb:volume-nr></sb:series><sb:date>1992</sb:date></sb:issue><sb:pages><sb:first-page>427</sb:first-page><sb:last-page>437</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib8"><ce:label>Chegini et al., 1993</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Chegini</ce:surname><ce:given-name>N.</ce:given-name></sb:author><sb:author><ce:surname>Rossi</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Schultz</ce:surname><ce:given-name>G.</ce:given-name></sb:author><sb:author><ce:surname>Dunn</ce:surname><ce:given-name>W.</ce:given-name></sb:author><sb:author><ce:surname>Masterson</ce:surname><ce:given-name>B.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Cellular distribution of epidermal growth factor, transforming growth factor-a, and epidermal growth factor receptor in fascia and peritoneum during healing in the rat: an autoradiographic and immunohistochemical study</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Wound Rep. Regen.</sb:maintitle></sb:title><sb:volume-nr>1</sb:volume-nr></sb:series><sb:date>1993</sb:date></sb:issue><sb:pages><sb:first-page>2840</sb:first-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib9"><ce:label>Chirgwin et al., 1979</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Chirgwin</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Przybyla</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>MacDonald</ce:surname><ce:given-name>R.</ce:given-name></sb:author><sb:author><ce:surname>Rutter</ce:surname><ce:given-name>W.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Isolation of biologically active ribonucleic acid from sources rich in ribonucleases</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Biochemistry</sb:maintitle></sb:title><sb:volume-nr>18</sb:volume-nr></sb:series><sb:date>1979</sb:date></sb:issue><sb:pages><sb:first-page>5294</sb:first-page><sb:last-page>5299</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib10"><ce:label>Cooper and MacQueen, 1983</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Cooper</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>MacQueen</ce:surname><ce:given-name>H.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Subunits of laminin are differentially synthesized in mouse eggs and early embryos</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Deve. Biol.</sb:maintitle></sb:title><sb:volume-nr>96</sb:volume-nr></sb:series><sb:date>1983</sb:date></sb:issue><sb:pages><sb:first-page>467</sb:first-page><sb:last-page>571</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib11"><ce:label>Cutler, 1989</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Cutler</ce:surname><ce:given-name>L.S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Functional differentiation of salivary glands</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:book-series><sb:editors><sb:editor><ce:surname>Forte</ce:surname><ce:given-name>J.</ce:given-name></sb:editor></sb:editors><sb:series><sb:title><sb:maintitle>Handbook of Physiology: Salivary, Pancreatic, Gastric, and Hepatobilliary Secretion</sb:maintitle></sb:title><sb:volume-nr>Vol. 3.</sb:volume-nr></sb:series></sb:book-series><sb:date>1989</sb:date><sb:publisher><sb:name>American Physiological Society Press</sb:name><sb:location>New York</sb:location></sb:publisher></sb:edited-book><sb:pages><sb:first-page>413</sb:first-page><sb:last-page>420</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib12"><ce:label>Cutler, 1990</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Cutler</ce:surname><ce:given-name>L.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>The role of extracellular matrix in the morphogenesis and differentiation of salivary glands</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Adv. dent. Res.</sb:maintitle></sb:title><sb:volume-nr>4</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>27</sb:first-page><sb:last-page>33</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib13"><ce:label>Fitch and Linsenmayer, 1994</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Fitch</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Linsenmayer</ce:surname><ce:given-name>T.</ce:given-name></sb:author></sb:authors></sb:contribution><sb:host><sb:edited-book><sb:editors><sb:editor><ce:surname>Yurchenco</ce:surname><ce:given-name>P.</ce:given-name></sb:editor><sb:editor><ce:surname>Birk</ce:surname><ce:given-name>O.</ce:given-name></sb:editor><sb:editor><ce:surname>Mecham</ce:surname><ce:given-name>R.</ce:given-name></sb:editor></sb:editors><sb:title><sb:maintitle>Interstitial basement membrane components in development. In Extracellular Matrix Assembly and Structure</sb:maintitle></sb:title><sb:date>1994</sb:date><sb:publisher><sb:name>Academic Press</sb:name><sb:location>New York</sb:location></sb:publisher></sb:edited-book><sb:pages><sb:first-page>441</sb:first-page><sb:last-page>462</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib14"><ce:label>Fukuda et al., 1988</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Fukuda</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Masua</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Kishi</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Harhimoto</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Taro</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Nogawa</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Nakanishi</ce:surname><ce:given-name>Y.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>The role of interstitial collagens in cleft formation of mouse embryonic submandibular gland during initial branching</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Development</sb:maintitle></sb:title><sb:volume-nr>103</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>259</sb:first-page><sb:last-page>267</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib15"><ce:label>Grobstein, 1953a</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Grobstein</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Analysis in vitro of the early organization of the rudiments of mouse submandibular gland</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Morph.</sb:maintitle></sb:title><sb:volume-nr>93</sb:volume-nr></sb:series><sb:date>1953</sb:date></sb:issue><sb:pages><sb:first-page>19</sb:first-page><sb:last-page>43</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib16"><ce:label>Grobstein, 1953b</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Grobstein</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Epithelial-mesenchymal specificity in the morphogenesis of mouse submandibular rudiments in vitro</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Exp. Zool.</sb:maintitle></sb:title><sb:volume-nr>124</sb:volume-nr></sb:series><sb:date>1953</sb:date></sb:issue><sb:pages><sb:first-page>383</sb:first-page><sb:last-page>413</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib17"><ce:label>Grobstein, 1953c</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Grobstein</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Morphogenetic interactions between embryonic tissues separated by a membrane filter</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Nature</sb:maintitle></sb:title><sb:volume-nr>172</sb:volume-nr></sb:series><sb:date>1953</sb:date></sb:issue><sb:pages><sb:first-page>869</sb:first-page><sb:last-page>871</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib18"><ce:label>Grobstein and Cohen, 1965</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Grobstein</ce:surname><ce:given-name>C.</ce:given-name></sb:author><sb:author><ce:surname>Cohen</ce:surname><ce:given-name>J.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Collagenase: effect on morphogenesis of embryonic salivary epithelium in vitro</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Science</sb:maintitle></sb:title><sb:volume-nr>150</sb:volume-nr></sb:series><sb:date>1965</sb:date></sb:issue><sb:pages><sb:first-page>626</sb:first-page><sb:last-page>628</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib19"><ce:label>Haqqi et al., 1992</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Haqqi</ce:surname><ce:given-name>T.</ce:given-name></sb:author><sb:author><ce:surname>Anderson</ce:surname><ce:given-name>G.</ce:given-name></sb:author><sb:author><ce:surname>Banerjee</ce:surname><ce:given-name>S.</ce:given-name></sb:author><sb:author><ce:surname>David</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Restricted heterogeneity in T-cell antigen receptor V beta gene usage in the lymph nodes and arthritic joints in mice</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:book-series><sb:series><sb:title><sb:maintitle>Proc. Natl Acad. Sci. USA</sb:maintitle></sb:title><sb:volume-nr>89</sb:volume-nr></sb:series></sb:book-series><sb:date>1992</sb:date></sb:edited-book><sb:pages><sb:first-page>1252</sb:first-page><sb:last-page>1255</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib20"><ce:label>Hardman and Spooner, 1992</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Hardman</ce:surname><ce:given-name>P.</ce:given-name></sb:author><sb:author><ce:surname>Spooner</ce:surname><ce:given-name>B.S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Localization of extracellular matrix components in developing mouse salivary glands by confocal microscopy</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Anat. Rec.</sb:maintitle></sb:title><sb:volume-nr>234</sb:volume-nr></sb:series><sb:date>1992</sb:date></sb:issue><sb:pages><sb:first-page>452</sb:first-page><sb:last-page>4549</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib21"><ce:label>Hintze, 1995</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Hintze</ce:surname><ce:given-name>J.L.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>NCSS 6.0 Statistical System for Windows</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:date>1995</sb:date><sb:publisher><sb:name>Kaysville</sb:name><sb:location>UT</sb:location></sb:publisher></sb:edited-book></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib22"><ce:label>Hoffman and Kleiman, 1996</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Hoffman</ce:surname><ce:given-name>M.P.</ce:given-name></sb:author><sb:author><ce:surname>Kleiman</ce:surname><ce:given-name>H.K.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Role of basement membrane in cell differentiation</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:editors><sb:editor><ce:surname>Karger</ce:surname><ce:given-name>S.</ce:given-name></sb:editor></sb:editors><sb:title><sb:maintitle>Contributions to Nephrology</sb:maintitle></sb:title><sb:date>1996</sb:date></sb:edited-book></sb:host><sb:comment>GA, Basel</sb:comment></sb:reference></ce:bib-reference><ce:bib-reference id="bib23"><ce:label>Kidd et al., 1991</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Kidd</ce:surname><ce:given-name>V.J.</ce:given-name></sb:author><sb:author><ce:surname>Luo</ce:surname><ce:given-name>W.</ce:given-name></sb:author><sb:author><ce:surname>Xiang</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Tu</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>Easton</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>McCune</ce:surname><ce:given-name>S.</ce:given-name></sb:author><sb:author><ce:surname>Snead</ce:surname><ce:given-name>M.S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Regulated expression of a cell division control-related protein kinase during development</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Cell Growth Differentiation</sb:maintitle></sb:title><sb:volume-nr>2</sb:volume-nr></sb:series><sb:date>1991</sb:date></sb:issue><sb:pages><sb:first-page>85</sb:first-page><sb:last-page>93</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib24"><ce:label>Kishi and Hayakawa, 1984</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Kishi</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Hayakawa</ce:surname><ce:given-name>T.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Purification and characterization of bovine dental pulp Colagenase inhibitor</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Biochem. (Tokyo)</sb:maintitle></sb:title><sb:volume-nr>96</sb:volume-nr></sb:series><sb:date>1984</sb:date></sb:issue><sb:pages><sb:first-page>395</sb:first-page><sb:last-page>404</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib25"><ce:label>Kratchowil et al., 1986</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Kratchowil</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Dziadek</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Löhler</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Harbers</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Jaenisch</ce:surname><ce:given-name>R.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Normal epithelial branching morphogenesis in the absence of collagen I</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Dev. Biol.</sb:maintitle></sb:title><sb:volume-nr>117</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>595</sb:first-page><sb:last-page>606</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib26"><ce:label>Lazowski et al., 1994</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Lazowski</ce:surname><ce:given-name>K.W.</ce:given-name></sb:author><sb:author><ce:surname>Mertz</ce:surname><ce:given-name>P.M.</ce:given-name></sb:author><sb:author><ce:surname>Redman</ce:surname><ce:given-name>R.S.</ce:given-name></sb:author><sb:author><ce:surname>Kousvelari</ce:surname><ce:given-name>E.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Temporal and spatial expression of laminin, collagen types IV and I and α6/β1 integrin receptor in the developing rat parotid gland</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Differentiation</sb:maintitle></sb:title><sb:volume-nr>56</sb:volume-nr></sb:series><sb:date>1994</sb:date></sb:issue><sb:pages><sb:first-page>75</sb:first-page><sb:last-page>82</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib27"><ce:label>Leivo et al., 1980</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Leivo</ce:surname><ce:given-name>I.</ce:given-name></sb:author><sb:author><ce:surname>Vaheri</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Timpl</ce:surname><ce:given-name>R.</ce:given-name></sb:author><sb:author><ce:surname>Wartiovaara</ce:surname><ce:given-name>J.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Appearance and distribution of collagens and laminins in the early mouse embryo</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Dev. Biol.</sb:maintitle></sb:title><sb:volume-nr>76</sb:volume-nr></sb:series><sb:date>1980</sb:date></sb:issue><sb:pages><sb:first-page>100</sb:first-page><sb:last-page>114</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib28"><ce:label>Nakanishi et al., 1986</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Nakanishi</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Sugiura</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>Kishi</ce:surname><ce:given-name>J-I.</ce:given-name></sb:author><sb:author><ce:surname>Hayakawa</ce:surname><ce:given-name>T.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Collagenase inhibitor stimulates cleft formation during early morphogenesis of mouse salivary gland</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Dev. Biol.</sb:maintitle></sb:title><sb:volume-nr>113</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>201</sb:first-page><sb:last-page>206</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib29"><ce:label>Nakanishi et al., 1988</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Nakanishi</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Nogawa</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Hasimoto</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Kishi</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Hayakawa</ce:surname><ce:given-name>T.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Accumulation of collagen III at the cleft points of developing mouse submandibular epithelium</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Development</sb:maintitle></sb:title><sb:volume-nr>104</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>51</sb:first-page><sb:last-page>59</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib30"><ce:label>Nakanishi and Ishii, 1989</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Nakanishi</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Ishii</ce:surname><ce:given-name>T.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Epithelial cell shape in mouse embryonic submandibular gland: modulation by extracellular matrix components</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>BioEssays</sb:maintitle></sb:title><sb:volume-nr>11</sb:volume-nr></sb:series><sb:date>1989</sb:date></sb:issue><sb:pages><sb:first-page>163</sb:first-page><sb:last-page>167</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib31"><ce:label>Nogawa and Mizuno, 1981</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Nogawa</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Mizuno</ce:surname><ce:given-name>T.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Mesenchymal control over elongation and branching morphogenesis in salivary gland development</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Embryol. Exp. Morphol.</sb:maintitle></sb:title><sb:volume-nr>81</sb:volume-nr></sb:series><sb:date>1981</sb:date></sb:issue><sb:pages><sb:first-page>209</sb:first-page><sb:last-page>221</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib32"><ce:label>Poulsen et al., 1986</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Poulsen</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Jakobsen</ce:surname><ce:given-name>B.K.</ce:given-name></sb:author><sb:author><ce:surname>Mikkelsen</ce:surname><ce:given-name>B.M.</ce:given-name></sb:author><sb:author><ce:surname>Harmark</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Nielsen</ce:surname><ce:given-name>J.T.</ce:given-name></sb:author><sb:author><ce:surname>Hjorth</ce:surname><ce:given-name>T.P.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Coordination of murine parotid secretory protein and salivary amylase expression</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>EMBO J.</sb:maintitle></sb:title><sb:volume-nr>5</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>1891</sb:first-page><sb:last-page>1896</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib33"><ce:label>Raeymaekers, 1994</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Raeymaekers</ce:surname><ce:given-name>L.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Comments on quantitative PCR</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Eur. Cyt. Network</sb:maintitle></sb:title><sb:volume-nr>5</sb:volume-nr></sb:series><sb:date>1994</sb:date></sb:issue><sb:pages><sb:first-page>57</sb:first-page><sb:last-page>60</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib34"><ce:label>Raeymaekers, 1995</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Raeymaekers</ce:surname><ce:given-name>L.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>A commentary on the practical applications of competitive PCR</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Genome Res.</sb:maintitle></sb:title><sb:volume-nr>5</sb:volume-nr></sb:series><sb:date>1995</sb:date></sb:issue><sb:pages><sb:first-page>91</sb:first-page><sb:last-page>94</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib35"><ce:label>Redman, 1987</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Redman</ce:surname><ce:given-name>R.S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Development of the salivary glands</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:editors><sb:editor><ce:surname>Sreebny</ce:surname><ce:given-name>L.N.</ce:given-name></sb:editor></sb:editors><sb:title><sb:maintitle>The Salivary System</sb:maintitle></sb:title><sb:date>1987</sb:date><sb:publisher><sb:name>CRC Press</sb:name><sb:location>Boca Raton, FL</sb:location></sb:publisher></sb:edited-book><sb:pages><sb:first-page>2</sb:first-page><sb:last-page>20</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib36"><ce:label>Rugh, 1968</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Rugh</ce:surname><ce:given-name>R.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>The mouse: its reproduction and development</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:title><sb:maintitle>The mouse: its reproduction and development</sb:maintitle></sb:title><sb:date>1968</sb:date><sb:publisher><sb:name>Burgess Publishing Co.</sb:name><sb:location>Minneapolis, MN</sb:location></sb:publisher></sb:edited-book><sb:pages><sb:first-page>102</sb:first-page><sb:last-page>208</sb:last-page></sb:pages></sb:host><sb:comment>Chap. 4</sb:comment></sb:reference></ce:bib-reference><ce:bib-reference id="bib37"><ce:label>Siebert, 1993</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Siebert</ce:surname><ce:given-name>P.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Quantitative RT-PCR</sb:maintitle></sb:title></sb:contribution><sb:host><sb:book><sb:date>1993</sb:date><sb:publisher><sb:name>Clontech Laboratories, Inc.</sb:name><sb:location>Palo Alto, CA</sb:location></sb:publisher></sb:book></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib38"><ce:label>Siebert and Larrick, 1992</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Siebert</ce:surname><ce:given-name>P.D.</ce:given-name></sb:author><sb:author><ce:surname>Larrick</ce:surname><ce:given-name>J.S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Competitive PCR</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Nature</sb:maintitle></sb:title><sb:volume-nr>359</sb:volume-nr></sb:series><sb:date>1992</sb:date></sb:issue><sb:pages><sb:first-page>557</sb:first-page><sb:last-page>558</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib39"><ce:label>Spooner and Faubion, 1980</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Spooner</ce:surname><ce:given-name>B.S.</ce:given-name></sb:author><sb:author><ce:surname>Faubion</ce:surname><ce:given-name>J.M.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Collagen involvement in branching morphogenesis of embryonic lung and salivary gland</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Dev. Biol.</sb:maintitle></sb:title><sb:volume-nr>77</sb:volume-nr></sb:series><sb:date>1980</sb:date></sb:issue><sb:pages><sb:first-page>84</sb:first-page><sb:last-page>102</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib40"><ce:label>Spooner et al., 1985</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Spooner</ce:surname><ce:given-name>B.S.</ce:given-name></sb:author><sb:author><ce:surname>Bassett</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Stokes</ce:surname><ce:given-name>B.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Sulfated glycosaminoglycan deposition and processing at the basal epithelial surface in branching and β-D-xyloside-inhibited embryonic salivary glands</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Dev. Biol.</sb:maintitle></sb:title><sb:volume-nr>109</sb:volume-nr></sb:series><sb:date>1985</sb:date></sb:issue><sb:pages><sb:first-page>177</sb:first-page><sb:last-page>183</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib41"><ce:label>Spooner et al., 1986</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Spooner</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Thompson-Pletsher</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Stokes</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Bassett</ce:surname><ce:given-name>K.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Extracellular matrix involvement in epithelial branching morphogenesis</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:book-series><sb:editors><sb:editor><ce:surname>Steinberg</ce:surname><ce:given-name>M.</ce:given-name></sb:editor></sb:editors><sb:series><sb:title><sb:maintitle>The Cell Surface in Development and Cancer</sb:maintitle></sb:title><sb:volume-nr>Vol. 3.</sb:volume-nr></sb:series></sb:book-series><sb:date>1986</sb:date><sb:publisher><sb:name>Plenum Press</sb:name><sb:location>New York</sb:location></sb:publisher></sb:edited-book><sb:pages><sb:first-page>225</sb:first-page><sb:last-page>260</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib42"><ce:label>Tarnuzzer et al., 1996</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Tarnuzzer</ce:surname><ce:given-name>R.</ce:given-name></sb:author><sb:author><ce:surname>Macauley</ce:surname><ce:given-name>S.</ce:given-name></sb:author><sb:author><ce:surname>Farmerie</ce:surname><ce:given-name>W.</ce:given-name></sb:author><sb:author><ce:surname>Cabellaro</ce:surname><ce:given-name>S.</ce:given-name></sb:author><sb:author><ce:surname>Ghassemifar</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Anderson</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Robinson</ce:surname><ce:given-name>C.</ce:given-name></sb:author><sb:author><ce:surname>Grant</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Humphreys-Beher</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Franzen</ce:surname><ce:given-name>L.</ce:given-name></sb:author><sb:author><ce:surname>Peck</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Schultz</ce:surname><ce:given-name>G.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Competitive RNA templates for detection and quantitation of growth factors, cytokines, extracellular matrix components, and matrix metalloproteinases by RT-PCR</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>BioTechniques</sb:maintitle></sb:title><sb:volume-nr>20</sb:volume-nr></sb:series><sb:date>1996</sb:date></sb:issue><sb:pages><sb:first-page>670</sb:first-page><sb:last-page>674</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib43"><ce:label>Thompsopn and Spooner, 1982</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Thompsopn</ce:surname><ce:given-name>H.A.</ce:given-name></sb:author><sb:author><ce:surname>Spooner</ce:surname><ce:given-name>B.S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Inhibition of branching morphogenesis and alteration of glycosaminoglycan biosynthesis in salivary glands treated with βD-xyloside</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Dev. Biol</sb:maintitle></sb:title><sb:volume-nr>89</sb:volume-nr></sb:series><sb:date>1982</sb:date></sb:issue><sb:pages><sb:first-page>417</sb:first-page><sb:last-page>424</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib44"><ce:label>Thompson and Spooner, 1983</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Thompson</ce:surname><ce:given-name>H.A.</ce:given-name></sb:author><sb:author><ce:surname>Spooner</ce:surname><ce:given-name>B.S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Proteoglycan and glycosaminoglycan synthesis in embryonic mouse salivary glands: effects of β-D-xyloside, an inhibitor of branching morphogenesis</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Cell Biol.</sb:maintitle></sb:title><sb:volume-nr>96</sb:volume-nr></sb:series><sb:date>1983</sb:date></sb:issue><sb:pages><sb:first-page>1443</sb:first-page><sb:last-page>1450</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib45"><ce:label>Trelstad, 1984</ce:label><sb:reference><sb:contribution><sb:authors><sb:author><ce:surname>Trelstad</ce:surname><ce:given-name>R.L.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>The role of extracellular matrix in development</sb:maintitle></sb:title></sb:contribution><sb:host><sb:book><sb:date>1984</sb:date><sb:publisher><sb:name>Alan R Liss</sb:name><sb:location>New York</sb:location></sb:publisher></sb:book></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Extracellular-matrix gene expression during mouse submandibular gland development</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Extracellular-matrix gene expression during mouse submandibular gland development</title></titleInfo><name type="personal"><namePart type="given">Shawn</namePart><namePart type="family">P. Macauley</namePart><affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>*Present address: Department of Genetics and Cell Biology, Health Research and Education Center, Washington State University at Spokane, Spokane, WA 99204- 0399, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Roy</namePart><namePart type="family">W. Tarnuzzer</namePart><affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gregory</namePart><namePart type="family">S. Schultz</namePart><affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nasser</namePart><namePart type="family">Chegini</namePart><affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gregory</namePart><namePart type="family">E. Oxford</namePart><affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Michael</namePart><namePart type="family">G. Humphreysbeher</namePart><affiliation>To whom all correspondence should be addressed, at the Department of Oral Biology.</affiliation><affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><affiliation>Center for Orphan Diseases, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1997</dateIssued><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</abstract><subject lang="en"><genre>Keywords</genre><topic>mouse submandibular gland development</topic><topic>quantitative RT-PCR</topic><topic>extracellular matrix</topic></subject><subject lang="en"><topic>DEPC : diethyl pyrocarbonate</topic><topic>dT : deoxythymidine</topic><topic>dNTP, rNTP : deoxy- (and ribo-)nucleoside triphosphate</topic><topic>RT-PCR : reverse transcription-polymerase chain reaction</topic></subject><relatedItem type="host"><titleInfo><title>Archives of Oral Biology</title></titleInfo><titleInfo type="abbreviated"><title>AOB</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1997</dateIssued></originInfo><identifier type="ISSN">0003-9969</identifier><identifier type="PII">S0003-9969(00)X0027-1</identifier><part><date>1997</date><detail type="volume"><number>42</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="issue-pages"><start>407</start><end>468</end></extent><extent unit="pages"><start>443</start><end>454</end></extent></part></relatedItem><identifier type="istex">8F9CD54D0D5B213E55E24A6109D79DA6370829DF</identifier><identifier type="ark">ark:/67375/6H6-Q9SG87X5-F</identifier><identifier type="DOI">10.1016/S0003-9969(97)00027-7</identifier><identifier type="PII">S0003-9969(97)00027-7</identifier><identifier type="ArticleID">97000277</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Q9SG87X5-F/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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