Extracellular-matrix gene expression during mouse submandibular gland development
Identifieur interne : 001F15 ( Istex/Corpus ); précédent : 001F14; suivant : 001F16Extracellular-matrix gene expression during mouse submandibular gland development
Auteurs : Shawn P. Macauley ; Roy W. Tarnuzzer ; Gregory S. Schultz ; Nasser Chegini ; Gregory E. Oxford ; Michael G. HumphreysbeherSource :
- Archives of Oral Biology [ 0003-9969 ] ; 1997.
English descriptors
- KwdEn :
- Teeft :
- Band intensities, Basal, Basal lamina, Basement membrane, Basement membranes, Biol, Cleft formation, Collagen, Collagen types, Ductal cells, Epithelial, Epithelial lobules, Epithelium, Extracellular, Extracellular matrix, Extracellular matrix components, Fetal, Fetal glands, Fetal submandibular glands, Fibronectin, Gene expression, Gland, Grobstein, Immunoreactive, Integrin receptor, Laminin, Lysyl, Lysyl oxidase, Macauley, Matrix, Mesenchymal, Mesenchymal capsule, Mesenchyme, Morphogenesis, Mouse submandibular gland, Mouse submandibular glands, Mrna, Nakanishi, Neonatal, Oligonucleotide, Oligonucleotide primers, Oral biology, Other days, Oxidase, Parotid, Parotid gland, Primary antibody, Primer, Receptor, Salivary, Salivary glands, Spooner, Striated ducts, Submandibular, Submandibular gland, Submandibular glands, Transcription reaction.
Abstract
Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.
Url:
DOI: 10.1016/S0003-9969(97)00027-7
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</div>
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<abstract xml:lang="en"><p>Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</p>
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</item>
<item><term>dNTP, rNTP</term>
<term>deoxy- (and ribo-)nucleoside triphosphate</term>
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<item><term>RT-PCR</term>
<term>reverse transcription-polymerase chain reaction</term>
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<head><ce:title>Extracellular-matrix gene expression during mouse submandibular gland development</ce:title>
<ce:author-group><ce:author><ce:given-name>Shawn</ce:given-name>
<ce:surname>P. Macauley</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>1</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="fn1"><ce:sup>*</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Roy</ce:given-name>
<ce:surname>W. Tarnuzzer</ce:surname>
<ce:cross-ref refid="aff2"><ce:sup>2</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="aff3"><ce:sup>3</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Gregory</ce:given-name>
<ce:surname>S. Schultz</ce:surname>
<ce:cross-ref refid="aff2"><ce:sup>2</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="aff3"><ce:sup>3</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Nasser</ce:given-name>
<ce:surname>Chegini</ce:surname>
<ce:cross-ref refid="aff2"><ce:sup>2</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="aff3"><ce:sup>3</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Gregory</ce:given-name>
<ce:surname>E. Oxford</ce:surname>
<ce:cross-ref refid="aff1"><ce:sup>1</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>Michael</ce:given-name>
<ce:surname>G. Humphreysbeher</ce:surname>
<ce:cross-ref refid="cor1"><ce:sup>†</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="aff1"><ce:sup>1</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="aff4"><ce:sup>4</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:affiliation id="aff1"><ce:label>a</ce:label>
<ce:textfn>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</ce:textfn>
</ce:affiliation>
<ce:affiliation id="aff2"><ce:label>b</ce:label>
<ce:textfn>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</ce:textfn>
</ce:affiliation>
<ce:affiliation id="aff3"><ce:label>c</ce:label>
<ce:textfn>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</ce:textfn>
</ce:affiliation>
<ce:affiliation id="aff4"><ce:label>d</ce:label>
<ce:textfn>Center for Orphan Diseases, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</ce:textfn>
</ce:affiliation>
<ce:correspondence id="cor1"><ce:label>†</ce:label>
<ce:text>To whom all correspondence should be addressed, at the Department of Oral Biology.</ce:text>
</ce:correspondence>
<ce:footnote id="fn1"><ce:label>*</ce:label>
<ce:note-para>Present address: Department of Genetics and Cell Biology, Health Research and Education Center, Washington State University at Spokane, Spokane, WA 99204- 0399, U.S.A.</ce:note-para>
</ce:footnote>
</ce:author-group>
<ce:date-accepted day="17" month="2" year="1997"></ce:date-accepted>
<ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para id="SP0005">Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
<ce:keywords class="keyword" xml:lang="en"><ce:section-title>Keywords</ce:section-title>
<ce:keyword><ce:text>mouse submandibular gland development</ce:text>
</ce:keyword>
<ce:keyword><ce:text>quantitative RT-PCR</ce:text>
</ce:keyword>
<ce:keyword><ce:text>extracellular matrix</ce:text>
</ce:keyword>
</ce:keywords>
<ce:keywords class="abr"><ce:keyword><ce:text>DEPC</ce:text>
<ce:keyword><ce:text>diethyl pyrocarbonate</ce:text>
</ce:keyword>
</ce:keyword>
<ce:keyword><ce:text>dT</ce:text>
<ce:keyword><ce:text>deoxythymidine</ce:text>
</ce:keyword>
</ce:keyword>
<ce:keyword><ce:text>dNTP, rNTP</ce:text>
<ce:keyword><ce:text>deoxy- (and ribo-)nucleoside triphosphate</ce:text>
</ce:keyword>
</ce:keyword>
<ce:keyword><ce:text>RT-PCR</ce:text>
<ce:keyword><ce:text>reverse transcription-polymerase chain reaction</ce:text>
</ce:keyword>
</ce:keyword>
</ce:keywords>
</head>
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<name type="personal"><namePart type="given">Shawn</namePart>
<namePart type="family">P. Macauley</namePart>
<affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
<affiliation>*Present address: Department of Genetics and Cell Biology, Health Research and Education Center, Washington State University at Spokane, Spokane, WA 99204- 0399, U.S.A.</affiliation>
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</name>
<name type="personal"><namePart type="given">Roy</namePart>
<namePart type="family">W. Tarnuzzer</namePart>
<affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
<affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Gregory</namePart>
<namePart type="family">S. Schultz</namePart>
<affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
<affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Nasser</namePart>
<namePart type="family">Chegini</namePart>
<affiliation>Department of Obstetrics and Gynecology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
<affiliation>Institute for Wound Research University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Gregory</namePart>
<namePart type="family">E. Oxford</namePart>
<affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
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</role>
</name>
<name type="personal"><namePart type="given">Michael</namePart>
<namePart type="family">G. Humphreysbeher</namePart>
<affiliation>To whom all correspondence should be addressed, at the Department of Oral Biology.</affiliation>
<affiliation>Department of Oral Biology, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
<affiliation>Center for Orphan Diseases, University of Florida College of Medicine, GainesvilleFL 32610, U.S.A.</affiliation>
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<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1997</dateIssued>
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<language><languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<abstract lang="en">Abstract: Early morphogenesis of mouse submandibular glands begins on late day 11 of fetal development when the epithelium begins to bud from the surrounding mandibular mesenchyme. Using total RNA collected from fetal BALB/c submandibular glands, steady-state levels of mRNA expression for extracellular matrix molecules were measured using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR). By comparing the PCR amplification products of both the cellular mRNA and a synthetic template, pMATRIX, it was possible to measure the direct expression of collagens α2(I), α1(111), αl(IV), fibronectin, laminin B2, elastin and lysyl oxidase genes. There was an observed trend for an increasing concentration of collagen α2(I), collagen al(III) and lysyl oxidase mRNA molecules per cell on day 16 of development. The relative abundance of elastin mRNA was detectable only on day 16. Fibronectin and laminin B2 were more constitutively present but had their highest copy number per cell on day 16. The presence of extracellular-matrix protein was confirmed by immunohislochemistry using day-16 fetal glands and adult glands. With the construction of the pMATRIX supertemplate and the advent of quantitative, competitive RT-PCR technology, it has been possible to measure small changes in the steady-state concentrations for extracellular-matrix mRNA during salivary gland development.</abstract>
<subject lang="en"><genre>Keywords</genre>
<topic>mouse submandibular gland development</topic>
<topic>quantitative RT-PCR</topic>
<topic>extracellular matrix</topic>
</subject>
<subject lang="en"><topic>DEPC : diethyl pyrocarbonate</topic>
<topic>dT : deoxythymidine</topic>
<topic>dNTP, rNTP : deoxy- (and ribo-)nucleoside triphosphate</topic>
<topic>RT-PCR : reverse transcription-polymerase chain reaction</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Archives of Oral Biology</title>
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<titleInfo type="abbreviated"><title>AOB</title>
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<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1997</dateIssued>
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<identifier type="ISSN">0003-9969</identifier>
<identifier type="PII">S0003-9969(00)X0027-1</identifier>
<part><date>1997</date>
<detail type="volume"><number>42</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>6</number>
<caption>no.</caption>
</detail>
<extent unit="issue-pages"><start>407</start>
<end>468</end>
</extent>
<extent unit="pages"><start>443</start>
<end>454</end>
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</part>
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<identifier type="ark">ark:/67375/6H6-Q9SG87X5-F</identifier>
<identifier type="DOI">10.1016/S0003-9969(97)00027-7</identifier>
<identifier type="PII">S0003-9969(97)00027-7</identifier>
<identifier type="ArticleID">97000277</identifier>
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