Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo
Identifieur interne : 001E71 ( Istex/Corpus ); précédent : 001E70; suivant : 001E72Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo
Auteurs : Radhia Gargouri ; Rajiv L. Joshi ; John F. Bol ; Suzanne Astier-Manifacier ; Anne-Lise HaenniSource :
- Virology [ 0042-6822 ] ; 1989.
English descriptors
- Teeft :
- Agarose, Chinese cabbage, Denaturing, Denaturing conditions, Dsrna, Dsrnas, Ethanol, Ethanol precipitation, Ethidium bromide staining, Genome, Genomic, Genomic length, Genomic size, Glyoxal, Haenni, Hybridization, Internal initiation, Joshi, Methylation, Mosaic, Mosaic virus, Nascent, Nascent subgenomic, Native conditions, Northern blot hybridization, Nucleic, Nucleic acids, Nucleotide, Nucleotide sequence, Penultimate nucleoside, Polynucleotide kinase, Putative subgenomic dsrna, Replication, Replicative, Rna, Rnase, Room temperature, Subgenomic, Subgenomic length, Subgenomic rnas, Total tymv, Tracer, Turnip, Tymv, Tymv genomic, Uninfected, Vaccinia, Vaccinia virus, Vaccinia virus preparation, Viral, Virology.
Abstract
Abstract: Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3′ proximal and is expressed in vivo via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized in vivo by internal initiation of replication on (−) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermeadites and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (−) RNA probes under denaturing conditions and (2) characterization of the 5′ ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2′-methyltransferase.
Url:
DOI: 10.1016/0042-6822(89)90606-5
Links to Exploration step
ISTEX:88C4B9E0BD6AFE3ED99BAD16FC70020EF5541D06Le document en format XML
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Box 9505, 2300 RA Leiden, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Astier Manifacier, Suzanne" sort="Astier Manifacier, Suzanne" uniqKey="Astier Manifacier S" first="Suzanne" last="Astier-Manifacier">Suzanne Astier-Manifacier</name><affiliation><mods:affiliation>Laboratoire de Phytopathologie, I.N.R.A., route de Saint-Cyr, 78000 Versailles, France</mods:affiliation></affiliation></author><author><name sortKey="Haenni, Anne Lise" sort="Haenni, Anne Lise" uniqKey="Haenni A" first="Anne-Lise" last="Haenni">Anne-Lise Haenni</name><affiliation><mods:affiliation>To whom requests for reprints should be addressed.</mods:affiliation></affiliation><affiliation><mods:affiliation>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">171</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="386">386</biblScope><biblScope unit="page" to="393">393</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Agarose</term><term>Chinese cabbage</term><term>Denaturing</term><term>Denaturing conditions</term><term>Dsrna</term><term>Dsrnas</term><term>Ethanol</term><term>Ethanol precipitation</term><term>Ethidium bromide staining</term><term>Genome</term><term>Genomic</term><term>Genomic length</term><term>Genomic size</term><term>Glyoxal</term><term>Haenni</term><term>Hybridization</term><term>Internal initiation</term><term>Joshi</term><term>Methylation</term><term>Mosaic</term><term>Mosaic virus</term><term>Nascent</term><term>Nascent subgenomic</term><term>Native conditions</term><term>Northern blot hybridization</term><term>Nucleic</term><term>Nucleic acids</term><term>Nucleotide</term><term>Nucleotide sequence</term><term>Penultimate nucleoside</term><term>Polynucleotide kinase</term><term>Putative subgenomic dsrna</term><term>Replication</term><term>Replicative</term><term>Rna</term><term>Rnase</term><term>Room temperature</term><term>Subgenomic</term><term>Subgenomic length</term><term>Subgenomic rnas</term><term>Total tymv</term><term>Tracer</term><term>Turnip</term><term>Tymv</term><term>Tymv genomic</term><term>Uninfected</term><term>Vaccinia</term><term>Vaccinia virus</term><term>Vaccinia virus preparation</term><term>Viral</term><term>Virology</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3′ proximal and is expressed in vivo via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized in vivo by internal initiation of replication on (−) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermeadites and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (−) RNA probes under denaturing conditions and (2) characterization of the 5′ ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2′-methyltransferase.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>subgenomic</json:string><json:string>genomic</json:string><json:string>dsrnas</json:string><json:string>tymv</json:string><json:string>dsrna</json:string><json:string>rna</json:string><json:string>rnase</json:string><json:string>hybridization</json:string><json:string>genomic length</json:string><json:string>viral</json:string><json:string>turnip</json:string><json:string>haenni</json:string><json:string>genome</json:string><json:string>glyoxal</json:string><json:string>agarose</json:string><json:string>total tymv</json:string><json:string>methylation</json:string><json:string>mosaic virus</json:string><json:string>virology</json:string><json:string>internal initiation</json:string><json:string>joshi</json:string><json:string>tracer</json:string><json:string>vaccinia</json:string><json:string>nucleic acids</json:string><json:string>denaturing</json:string><json:string>nascent subgenomic</json:string><json:string>genomic size</json:string><json:string>native conditions</json:string><json:string>replicative</json:string><json:string>uninfected</json:string><json:string>chinese cabbage</json:string><json:string>nascent</json:string><json:string>ethanol</json:string><json:string>ethidium bromide staining</json:string><json:string>denaturing conditions</json:string><json:string>subgenomic length</json:string><json:string>northern blot hybridization</json:string><json:string>nucleic</json:string><json:string>mosaic</json:string><json:string>vaccinia virus</json:string><json:string>nucleotide sequence</json:string><json:string>ethanol precipitation</json:string><json:string>replication</json:string><json:string>nucleotide</json:string><json:string>vaccinia virus preparation</json:string><json:string>putative subgenomic dsrna</json:string><json:string>polynucleotide kinase</json:string><json:string>subgenomic rnas</json:string><json:string>tymv genomic</json:string><json:string>penultimate nucleoside</json:string><json:string>room temperature</json:string></teeft></keywords><author><json:item><name>Radhia Gargouri</name><affiliations><json:string>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</json:string></affiliations></json:item><json:item><name>Rajiv L. Joshi</name><affiliations><json:string>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</json:string></affiliations></json:item><json:item><name>John F. Bol</name><affiliations><json:string>Department of Biochemistry, State University of Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands</json:string></affiliations></json:item><json:item><name>Suzanne Astier-Manifacier</name><affiliations><json:string>Laboratoire de Phytopathologie, I.N.R.A., route de Saint-Cyr, 78000 Versailles, France</json:string></affiliations></json:item><json:item><name>Anne-Lise Haenni</name><affiliations><json:string>To whom requests for reprints should be addressed.</json:string><json:string>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-R36KM6SV-T</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3′ proximal and is expressed in vivo via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized in vivo by internal initiation of replication on (−) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermeadites and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (−) RNA probes under denaturing conditions and (2) characterization of the 5′ ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2′-methyltransferase.</abstract><qualityIndicators><score>8.884</score><pdfWordCount>5770</pdfWordCount><pdfCharCount>34528</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>157</abstractWordCount><abstractCharCount>1074</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo</title><pmid><json:string>2763458</json:string></pmid><pii><json:string>0042-6822(89)90606-5</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. 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Filipowicz</json:string><json:string>C. Michon-Dubuts</json:string><json:string>F. Chapeville</json:string><json:string>L. Pinck</json:string><json:string>M. Linthrost</json:string><json:string>Saran Wrap</json:string><json:string>K. Moldave</json:string><json:string>S. Joshi</json:string></persName><placeName><json:string>Leiden</json:string><json:string>Versailles</json:string><json:string>Strasbourg</json:string><json:string>France</json:string><json:string>Netherlands</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Langereis, 1987</json:string><json:string>Gould and Francki, 1981</json:string><json:string>Pleij et al., 1976</json:string><json:string>Laskey and Mills, 1975</json:string><json:string>Silberklang et al., 1977</json:string><json:string>Morris and Dodds (1979)</json:string><json:string>Barbosa and Moss, 1978</json:string><json:string>Joshi et al., 1986</json:string><json:string>Miller and Hall, 1984</json:string><json:string>Richardson, 1965</json:string><json:string>Joshi and Haenni, 1980</json:string><json:string>Peacock and Dingman, 1968</json:string><json:string>March et al., 1987a, 1988</json:string><json:string>Hardy et al</json:string><json:string>Briand et al., 1977</json:string><json:string>Porter et al., 1974</json:string><json:string>Joshi et al. (1978)</json:string><json:string>Loesch-Fries and Hall, 1980</json:string><json:string>Nassuth and Bol, 1983</json:string><json:string>Moss, 1977</json:string><json:string>MouchBs et al., 1984</json:string><json:string>Jaspars et al., 1985</json:string><json:string>Filipowicz and Haenni, 1978</json:string><json:string>Guilley and Briand, 1978</json:string><json:string>Bonner and Laskey, 1974</json:string><json:string>McMaster and Carmichael (1977)</json:string><json:string>Watanabe and Okada, 1986</json:string><json:string>Jordan and Dodds, 1985</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-R36KM6SV-T</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - phytopathologie. zoologie agricole. protection des cultures et des forets</json:string></inist></categories><publicationDate>1989</publicationDate><copyrightDate>1989</copyrightDate><doi><json:string>10.1016/0042-6822(89)90606-5</json:string></doi><id>88C4B9E0BD6AFE3ED99BAD16FC70020EF5541D06</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-R36KM6SV-T/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-R36KM6SV-T/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-R36KM6SV-T/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1989</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo</title><author xml:id="author-0000"><persName><forename type="first">Radhia</forename><surname>Gargouri</surname></persName><affiliation>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Rajiv L.</forename><surname>Joshi</surname></persName><affiliation>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">John F.</forename><surname>Bol</surname></persName><affiliation>Department of Biochemistry, State University of Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Suzanne</forename><surname>Astier-Manifacier</surname></persName><affiliation>Laboratoire de Phytopathologie, I.N.R.A., route de Saint-Cyr, 78000 Versailles, France</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Anne-Lise</forename><surname>Haenni</surname></persName><affiliation>To whom requests for reprints should be addressed.</affiliation><affiliation>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</affiliation></author><idno type="istex">88C4B9E0BD6AFE3ED99BAD16FC70020EF5541D06</idno><idno type="ark">ark:/67375/6H6-R36KM6SV-T</idno><idno type="DOI">10.1016/0042-6822(89)90606-5</idno><idno type="PII">0042-6822(89)90606-5</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0671-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989"></date><biblScope unit="volume">171</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="386">386</biblScope><biblScope unit="page" to="393">393</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1989</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3′ proximal and is expressed in vivo via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized in vivo by internal initiation of replication on (−) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermeadites and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (−) RNA probes under denaturing conditions and (2) characterization of the 5′ ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2′-methyltransferase.</p></abstract></profileDesc><revisionDesc><change when="1989">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-R36KM6SV-T/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>89906065</aid><ce:pii>0042-6822(89)90606-5</ce:pii><ce:doi>10.1016/0042-6822(89)90606-5</ce:doi><ce:copyright type="unknown" year="1989"></ce:copyright></item-info><head><ce:title>Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA <ce:italic>in vivo</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>Radhia</ce:given-name><ce:surname>Gargouri</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Rajiv L.</ce:given-name><ce:surname>Joshi</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>John F.</ce:given-name><ce:surname>Bol</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Suzanne</ce:given-name><ce:surname>Astier-Manifacier</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>‡</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Anne-Lise</ce:given-name><ce:surname>Haenni</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Institut Jacques Monod<ce:cross-ref refid="FN1"><ce:sup>2</ce:sup></ce:cross-ref><ce:footnote id="FN1"><ce:label>2</ce:label><ce:note-para>The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”</ce:note-para></ce:footnote>, 2 Place Jussieu, 75251 Paris Cedex 05, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Biochemistry, State University of Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Laboratoire de Phytopathologie, I.N.R.A., route de Saint-Cyr, 78000 Versailles, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom requests for reprints should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="21" month="10" year="1988"></ce:date-received><ce:date-accepted day="28" month="3" year="1989"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3′ proximal and is expressed <ce:italic>in vivo</ce:italic> via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized <ce:italic>in vivo</ce:italic> by internal initiation of replication on (−) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermeadites and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (−) RNA probes under denaturing conditions and (2) characterization of the 5′ ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2′-methyltransferase.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA</title></titleInfo><name type="personal"><namePart type="given">Radhia</namePart><namePart type="family">Gargouri</namePart><affiliation>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rajiv L.</namePart><namePart type="family">Joshi</namePart><affiliation>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">John F.</namePart><namePart type="family">Bol</namePart><affiliation>Department of Biochemistry, State University of Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Suzanne</namePart><namePart type="family">Astier-Manifacier</namePart><affiliation>Laboratoire de Phytopathologie, I.N.R.A., route de Saint-Cyr, 78000 Versailles, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Anne-Lise</namePart><namePart type="family">Haenni</namePart><affiliation>To whom requests for reprints should be addressed.</affiliation><affiliation>Institut Jacques Monod2 2 The Institut Jacques Monod is an “Institut Mixte, CNRS/Université Paris VII.”, 2 Place Jussieu, 75251 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued><copyrightDate encoding="w3cdtf">1989</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Turnip yellow mosaic virus (TYMV) possesses a monopartite single-stranded (+) sense RNA genome in which the coat protein (cp) gene is 3′ proximal and is expressed in vivo via a subgenomic RNA. Evidence is presented here that this subgenomic RNA is synthesized in vivo by internal initiation of replication on (−) RNA strands of genomic length. The double-stranded RNAs (dsRNAs) from TYMV-infected plants have been isolated, purified, and characterized. Under native conditions, no dsRNAs (replicative intermeadites and/or replicative forms) of subgenomic length corresponding to subgenomic cp RNA can be detected by ethidium bromide staining of RNA-sizing gels or by Northern blot hybridization using RNA probes. The presence of nascent subgenomic cp (+) RNA strands on the dsRNA of genomic length has been demonstrated using two different approaches: (1) Northern blot hybridization using (−) RNA probes under denaturing conditions and (2) characterization of the 5′ ends of nascent (+) RNA strands upon labeling by vaccinia virus nucleoside-2′-methyltransferase.</abstract><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1989</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0671-X</identifier><part><date>1989</date><detail type="volume"><number>171</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>319</start><end>651</end></extent><extent unit="pages"><start>386</start><end>393</end></extent></part></relatedItem><identifier type="istex">88C4B9E0BD6AFE3ED99BAD16FC70020EF5541D06</identifier><identifier type="ark">ark:/67375/6H6-R36KM6SV-T</identifier><identifier type="DOI">10.1016/0042-6822(89)90606-5</identifier><identifier type="PII">0042-6822(89)90606-5</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-R36KM6SV-T/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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