Viral spliced RNA are produced, encapsidated and reverse transcribed during in Vivo woodchuck hepatitis virus infection
Identifieur interne : 001D84 ( Istex/Corpus ); précédent : 001D83; suivant : 001D85Viral spliced RNA are produced, encapsidated and reverse transcribed during in Vivo woodchuck hepatitis virus infection
Auteurs : Olivier Hantz ; Isabelle Baginski ; Isabelle Fourel ; Isabelle Chemin ; Christian TrepoSource :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Academic press, Acceptor, Acceptor site, Amplification, Amplification primers, Cauliflower mosaic virus, Cdna, Cdna synthesis, Cetus corp, Consensus sequence, Core gene, Core particles, Duck hepatitis, Final volume, Fusion protein, Gene product, Genetic analysis, Genome, Ground squirrel hepatitis virus, Hantz, Hepatitis, Human hepatitis, Human liver tissues, Hybridization, Important role, Initiation codon, Lesser extent, Life cycle, Liver samples, Negative control, Noninfected woodchuck, Northern blot analysis, Nucleotide sequence, Oligonucleotide, Oligonucleotide probe, Oligonucleotide probes, Open reading frames, Poly, Polymerase, Polymerase chain reaction, Positive control, Potential translation product, Pregenomic, Primer, Primer pair, Probe, Rna, Schaller, Sequence analysis, Southern blot analysis, Spacer region, Splice, Splice donor site, Splice junction, Stringent conditions, Surface antigen, Suzuki, Terminal protein, Terminal protein domain, Transcript, Transcriptase, Transcription, Transcription step, Viral, Viral particles, Woodchuck, Woodchuck hepatitis virus, Woodchuck liver, Woodchuck liver tissues.
Abstract
Abstract: By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection.
Url:
DOI: 10.1016/0042-6822(92)91205-9
Links to Exploration step
ISTEX:2480042F84D5719172565A6066AE912985948A53Le document en format XML
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extent</term><term>Life cycle</term><term>Liver samples</term><term>Negative control</term><term>Noninfected woodchuck</term><term>Northern blot analysis</term><term>Nucleotide sequence</term><term>Oligonucleotide</term><term>Oligonucleotide probe</term><term>Oligonucleotide probes</term><term>Open reading frames</term><term>Poly</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Positive control</term><term>Potential translation product</term><term>Pregenomic</term><term>Primer</term><term>Primer pair</term><term>Probe</term><term>Rna</term><term>Schaller</term><term>Sequence analysis</term><term>Southern blot analysis</term><term>Spacer region</term><term>Splice</term><term>Splice donor site</term><term>Splice junction</term><term>Stringent conditions</term><term>Surface antigen</term><term>Suzuki</term><term>Terminal protein</term><term>Terminal protein domain</term><term>Transcript</term><term>Transcriptase</term><term>Transcription</term><term>Transcription step</term><term>Viral</term><term>Viral particles</term><term>Woodchuck</term><term>Woodchuck hepatitis virus</term><term>Woodchuck liver</term><term>Woodchuck liver tissues</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>primer</json:string><json:string>woodchuck</json:string><json:string>viral</json:string><json:string>genome</json:string><json:string>transcriptase</json:string><json:string>cdna</json:string><json:string>pregenomic</json:string><json:string>polymerase</json:string><json:string>poly</json:string><json:string>oligonucleotide</json:string><json:string>hybridization</json:string><json:string>hantz</json:string><json:string>splice junction</json:string><json:string>schaller</json:string><json:string>splice</json:string><json:string>rna</json:string><json:string>acceptor</json:string><json:string>terminal protein</json:string><json:string>oligonucleotide probe</json:string><json:string>duck hepatitis</json:string><json:string>hepatitis</json:string><json:string>spacer region</json:string><json:string>negative control</json:string><json:string>cdna synthesis</json:string><json:string>positive control</json:string><json:string>southern blot analysis</json:string><json:string>acceptor site</json:string><json:string>transcription</json:string><json:string>suzuki</json:string><json:string>human hepatitis</json:string><json:string>woodchuck liver tissues</json:string><json:string>woodchuck hepatitis virus</json:string><json:string>academic press</json:string><json:string>splice donor site</json:string><json:string>human liver tissues</json:string><json:string>northern blot analysis</json:string><json:string>probe</json:string><json:string>initiation codon</json:string><json:string>gene product</json:string><json:string>genetic analysis</json:string><json:string>important role</json:string><json:string>liver samples</json:string><json:string>polymerase chain reaction</json:string><json:string>ground squirrel hepatitis virus</json:string><json:string>surface antigen</json:string><json:string>oligonucleotide probes</json:string><json:string>cetus corp</json:string><json:string>final volume</json:string><json:string>stringent conditions</json:string><json:string>lesser extent</json:string><json:string>transcription step</json:string><json:string>cauliflower mosaic virus</json:string><json:string>open reading frames</json:string><json:string>sequence analysis</json:string><json:string>life cycle</json:string><json:string>terminal protein domain</json:string><json:string>woodchuck liver</json:string><json:string>fusion protein</json:string><json:string>core gene</json:string><json:string>primer pair</json:string><json:string>noninfected woodchuck</json:string><json:string>nucleotide sequence</json:string><json:string>consensus sequence</json:string><json:string>viral particles</json:string><json:string>potential translation product</json:string><json:string>core particles</json:string><json:string>amplification primers</json:string><json:string>amplification</json:string><json:string>transcript</json:string></teeft></keywords><author><json:item><name>Olivier Hantz</name><affiliations><json:string>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</json:string></affiliations></json:item><json:item><name>Isabelle Baginski</name><affiliations><json:string>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</json:string></affiliations></json:item><json:item><name>Isabelle Fourel</name><affiliations><json:string>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</json:string></affiliations></json:item><json:item><name>Isabelle Chemin</name><affiliations><json:string>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</json:string></affiliations></json:item><json:item><name>Christian Trepo</name><affiliations><json:string>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-S7SVV68M-Z</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection.</abstract><qualityIndicators><score>8.67</score><pdfWordCount>4438</pdfWordCount><pdfCharCount>29886</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>575.84 x 792.28 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>186</abstractWordCount><abstractCharCount>1196</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Viral spliced RNA are produced, encapsidated and reverse transcribed during in Vivo woodchuck hepatitis virus infection</title><pmid><json:string>1382340</json:string></pmid><pii><json:string>0042-6822(92)91205-9</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. 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USA</title><language><json:string>unknown</json:string></language><volume>83</volume><pages><first>2531</first><last>2535</last></pages></serie><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1992</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0705-2</json:string></pii><volume>190</volume><issue>1</issue><pages><first>193</first><last>200</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1992</json:string><json:string>1300</json:string><json:string>1800</json:string></date><geogName><json:string>region of the Pol</json:string></geogName><orgName><json:string>Cetus Corp</json:string><json:string>Biotech</json:string><json:string>Pharmacia LKB Biotechnology</json:string><json:string>United States Biochemical</json:string><json:string>Foundation</json:string><json:string>Bethesda Research Laboratories, Inc</json:string><json:string>Cetus Corp.</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>I. Fourel</json:string><json:string>Chantal Jacquet</json:string></persName><placeName><json:string>Mannheim</json:string><json:string>American</json:string><json:string>Norwalk</json:string><json:string>CT</json:string><json:string>France</json:string><json:string>Mordy</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Mot-By et al., 1985</json:string><json:string>Hantz et al., 1984</json:string><json:string>Li et al., 1989</json:string><json:string>Huang and Summers, 1991</json:string><json:string>EMBL sequence data bank library release 27 May, 1991</json:string><json:string>Schlicht et al., 1989</json:string><json:string>Suzuki et al., 1989, 1990</json:string><json:string>Radziwill et al., 1990</json:string><json:string>Enders et al</json:string><json:string>Enders et al., 1985</json:string><json:string>Wu et al., 1991a</json:string><json:string>Bosch et al</json:string><json:string>Galibert et al., 1982</json:string><json:string>Chang et al., 1990</json:string><json:string>Robinson, 1990</json:string><json:string>Mann and Baltimore, 1985</json:string><json:string>Staden, 1984</json:string><json:string>Will et al., 1987</json:string><json:string>Miller and Robinson, 1986</json:string><json:string>Chang et al., 1989</json:string><json:string>Stacey, 1980</json:string><json:string>Wu et al., 1991 a</json:string><json:string>Bodor and Svoboda, 1989</json:string><json:string>Fox Chase Cancer Center, 7701</json:string><json:string>Su et al., 1989</json:string><json:string>Summers and Mason, 1982</json:string><json:string>Bartenschlager and Schaller, 1988</json:string><json:string>Glisin et al., 1974</json:string><json:string>Dessen et al., 1990</json:string><json:string>Suzuki et al., 1990</json:string><json:string>Ganem and Varmus, 1987</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-S7SVV68M-Z</json:string></ark><categories><wos><json:string>1 - 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The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection.</p></abstract></profileDesc><revisionDesc><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-S7SVV68M-Z/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>92912059</aid><ce:pii>0042-6822(92)91205-9</ce:pii><ce:doi>10.1016/0042-6822(92)91205-9</ce:doi><ce:copyright type="unknown" year="1992"></ce:copyright></item-info><head><ce:title>Viral spliced RNA are produced, encapsidated and reverse transcribed during <ce:italic>in Vivo</ce:italic> woodchuck hepatitis virus infection</ce:title><ce:author-group><ce:author><ce:given-name>Olivier</ce:given-name><ce:surname>Hantz</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Isabelle</ce:given-name><ce:surname>Baginski</ce:surname></ce:author><ce:author><ce:given-name>Isabelle</ce:given-name><ce:surname>Fourel</ce:surname><ce:cross-ref refid="FN1"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Isabelle</ce:given-name><ce:surname>Chemin</ce:surname></ce:author><ce:author><ce:given-name>Christian</ce:given-name><ce:surname>Trepo</ce:surname></ce:author><ce:affiliation><ce:textfn>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom reprint requests should be addressed.</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>2</ce:label><ce:note-para>Present address: Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="6" month="3" year="1992"></ce:date-received><ce:date-accepted day="22" month="5" year="1992"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Viral spliced RNA are produced, encapsidated and reverse transcribed during in Vivo woodchuck hepatitis virus infection</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Viral spliced RNA are produced, encapsidated and reverse transcribed during</title></titleInfo><name type="personal"><namePart type="given">Olivier</namePart><namePart type="family">Hantz</namePart><affiliation>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</affiliation><description>To whom reprint requests should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabelle</namePart><namePart type="family">Baginski</namePart><affiliation>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabelle</namePart><namePart type="family">Fourel</namePart><affiliation>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</affiliation><description>Present address: Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabelle</namePart><namePart type="family">Chemin</namePart><affiliation>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christian</namePart><namePart type="family">Trepo</namePart><affiliation>INSERM U271, 151, cours Albert Thomas, 69424 Lyon, Cedex 03, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and partially sequenced. The splicing donor and acceptor sites of this novel RNA are located, respectively, 130 nucleotides downstream of the ATG initiation codon of the core gene and 21 nucleotides upstream of the initiation codon of the pre-S2 surface gene. The splicing event generates a new core-polymerase fusion protein and removes the terminal protein domain and the spacer region of the polymerase gene. A nucleotide probe specific for the splice junction was used following RT-PCR, to further confirm the existence of this spliced RNA in the liver of seven WHV-infected woodchucks. Deleted viral DNA molecules corresponding to the 2.4 kb spliced RNA were also detected in the liver and, to a lesser extent, in the serum of infected woodchucks, suggesting that this spliced RNA can be encapsidated and reverse-transcribed during the course of natural WHV infection.</abstract><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0705-2</identifier><part><date>1992</date><detail type="volume"><number>190</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>567</end></extent><extent unit="pages"><start>193</start><end>200</end></extent></part></relatedItem><identifier type="istex">2480042F84D5719172565A6066AE912985948A53</identifier><identifier type="ark">ark:/67375/6H6-S7SVV68M-Z</identifier><identifier type="DOI">10.1016/0042-6822(92)91205-9</identifier><identifier type="PII">0042-6822(92)91205-9</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-S7SVV68M-Z/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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