Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea
Identifieur interne : 001985 ( Istex/Corpus ); précédent : 001984; suivant : 001986Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea
Auteurs : Go Atsumi ; Kenji S. Nakahara ; Tomoko Sugikawa Wada ; Sun Hee Choi ; Chikara Masuta ; Ichiro UyedaSource :
- Archives of Virology [ 0304-8608 ] ; 2012-06-01.
Abstract
Abstract: Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.
Url:
DOI: 10.1007/s00705-012-1281-3
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Nakahara</name><affiliation><mods:affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Wada, Tomoko Sugikawa" sort="Wada, Tomoko Sugikawa" uniqKey="Wada T" first="Tomoko Sugikawa" last="Wada">Tomoko Sugikawa Wada</name><affiliation><mods:affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Choi, Sun Hee" sort="Choi, Sun Hee" uniqKey="Choi S" first="Sun Hee" last="Choi">Sun Hee Choi</name><affiliation><mods:affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Masuta, Chikara" sort="Masuta, Chikara" uniqKey="Masuta C" first="Chikara" last="Masuta">Chikara Masuta</name><affiliation><mods:affiliation>Laboratory of Cell Biology and Manipulation, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Uyeda, Ichiro" sort="Uyeda, Ichiro" uniqKey="Uyeda I" first="Ichiro" last="Uyeda">Ichiro Uyeda</name><affiliation><mods:affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: uyeda@res.agr.hokudai.ac.jp</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="sub">Official Journal of the Virology Division of the International Union of Microbiological Societies</title><title level="j" type="abbrev">Arch Virol</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer Vienna</publisher><pubPlace>Vienna</pubPlace><date type="published" when="2012-06-01">2012-06-01</date><biblScope unit="volume">157</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1019">1019</biblScope><biblScope unit="page" to="1028">1028</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Go Atsumi</name><affiliations><json:string>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</json:string></affiliations></json:item><json:item><name>Kenji S. 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Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. 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Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Virology</term></item><item><term>Infectious Diseases</term></item><item><term>Medical Microbiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2011-11-07">Created</change><change when="2012-06-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-7RS5CL2R-0/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ 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Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Go</GivenName><FamilyName>Atsumi</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Kenji</GivenName><GivenName>S.</GivenName><FamilyName>Nakahara</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Tomoko</GivenName><GivenName>Sugikawa</GivenName><FamilyName>Wada</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Sun</GivenName><GivenName>Hee</GivenName><FamilyName>Choi</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Chikara</GivenName><FamilyName>Masuta</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Ichiro</GivenName><FamilyName>Uyeda</FamilyName></AuthorName><Contact><Phone>+81-11-7062473</Phone><Fax>+81-11-7062483</Fax><Email>uyeda@res.agr.hokudai.ac.jp</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Pathogen-Plant Interactions Group, Graduate School of Agriculture</OrgDivision><OrgName>Hokkaido University</OrgName><OrgAddress><City>Sapporo</City><Postcode>060-8589</Postcode><Country Code="JP">Japan</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Laboratory of Cell Biology and Manipulation, Graduate School of Agriculture</OrgDivision><OrgName>Hokkaido University</OrgName><OrgAddress><City>Sapporo</City><Postcode>060-8589</Postcode><Country Code="JP">Japan</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in <Emphasis Type="Italic">Nicotiana benthamiana</Emphasis>. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.</Para></Abstract><ArticleNote Type="ESMHint"><Heading>Electronic supplementary material</Heading><SimplePara>The online version of this article (doi:<ExternalRef><RefSource>10.1007/s00705-012-1281-3</RefSource><RefTarget Address="10.1007/s00705-012-1281-3" TargetType="DOI"></RefTarget></ExternalRef>) contains supplementary material, which is available to authorized users.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea</title></titleInfo><name type="personal"><namePart type="given">Go</namePart><namePart type="family">Atsumi</namePart><affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kenji</namePart><namePart type="given">S.</namePart><namePart type="family">Nakahara</namePart><affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tomoko</namePart><namePart type="given">Sugikawa</namePart><namePart type="family">Wada</namePart><affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sun</namePart><namePart type="given">Hee</namePart><namePart type="family">Choi</namePart><affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Chikara</namePart><namePart type="family">Masuta</namePart><affiliation>Laboratory of Cell Biology and Manipulation, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Ichiro</namePart><namePart type="family">Uyeda</namePart><affiliation>Pathogen-Plant Interactions Group, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan</affiliation><affiliation>E-mail: uyeda@res.agr.hokudai.ac.jp</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer Vienna</publisher><place><placeTerm type="text">Vienna</placeTerm></place><dateCreated encoding="w3cdtf">2011-11-07</dateCreated><dateIssued encoding="w3cdtf">2012-06-01</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pro (HC-Pro-D193Y) drastically attenuated the virulence of clover yellow vein virus (ClYVV) in legume plants. Furthermore, RNA-silencing suppression (RSS) activity of HC-Pro-D193Y was significantly reduced in Nicotiana benthamiana. Here, we examine the effect of expression of heterologous suppressors of RNA silencing, i.e., tomato bushy stunt virus p19, cucumber mosaic virus 2b, and their mutants, on the virulence of the ClYVV point mutant with D193Y (Cl-D193Y) in pea. P19 and 2b fully and partially complemented Cl-D193Y multiplication and virulence, including lethal systemic HR in pea, respectively, but the P19 and 2b mutants with defects in their RSS activity did not. Our findings strongly suggest that the D193Y mutation exclusively affects RSS activity of HC-Pro and that RSS activity is necessary for ClYVV multiplication and virulence in pea.</abstract><note>Original Article</note><relatedItem type="host"><titleInfo><title>Archives of Virology</title><subTitle>Official Journal of the Virology Division of the International Union of Microbiological Societies</subTitle></titleInfo><titleInfo type="abbreviated"><title>Arch Virol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2012-05-31</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Virology</topic><topic>Infectious Diseases</topic><topic>Medical Microbiology</topic></subject><identifier type="ISSN">0304-8608</identifier><identifier type="eISSN">1432-8798</identifier><identifier type="JournalID">705</identifier><identifier type="IssueArticleCount">28</identifier><identifier type="VolumeIssueCount">12</identifier><part><date>2012</date><detail type="volume"><number>157</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="pages"><start>1019</start><end>1028</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 2012</recordOrigin></recordInfo></relatedItem><identifier type="istex">98AE440EF494FF641B2D4826F89B3B2071D18D6A</identifier><identifier type="ark">ark:/67375/VQC-7RS5CL2R-0</identifier><identifier type="DOI">10.1007/s00705-012-1281-3</identifier><identifier type="ArticleID">1281</identifier><identifier type="ArticleID">s00705-012-1281-3</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 2012</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer-Verlag, 2012</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-7RS5CL2R-0/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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