Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin
Identifieur interne : 001754 ( Istex/Corpus ); précédent : 001753; suivant : 001755Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin
Auteurs : Michio Ohba ; Max D. Summers ; Pepper Hoops ; Gale E. SmithSource :
- Journal of Invertebrate Pathology [ 0022-2011 ] ; 1977.
English descriptors
- Teeft :
- Academic press, Acmnpv, Acmnpv loval, Acmnpv loval antiserum, Acmnpv polyhedrin, Antibody titers, Antiserum, Assay, Baculovirus, Baculoviruses, Bergold, Bombyx mori, Cell culture, Comparative studies, Etude comparee, Granulosis virus, Immunoradiometric assay, Inclusion body proteins, Individual wells, Insect pathol, Invertebrate tissue culture, Krywienczyk, Loval, Microtiter, Microtiter plates, Microtiter radioimmunoassay, Microtiter wells, Nonoccluded virus, Nuclear polyhedrosis virus, Nuclear polyhedrosis viruses, Nucleocapsids, Optimal conditions, Pathol, Phosphate buffer, Pieris brassicae, Polyhedrin, Polyhedrin antisera, Polyhedrosis, Preimmune serum, Radioimmunoassay, Serological, Serological characterization, Serological relationship, Serological relationships, Serological studies, Serum dilution, Similar experiments, Sodium azide, Specific activity, Specific antibodies, Viral, Viral antigens, Viral proteins, Volkman, Znvertebr.
Abstract
Abstract: A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. A preliminary study also demonstrated the ability to use this assay to detect specific antiviral antibodies.
Url:
DOI: 10.1016/0022-2011(77)90146-X
Links to Exploration step
ISTEX:A7DD56A230FA83DA829B5C6B676D7EA7B31CE051Le document en format XML
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Smith</name><affiliation><mods:affiliation>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Invertebrate Pathology</title><title level="j" type="abbrev">YJIPA</title><idno type="ISSN">0022-2011</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1977">1977</date><biblScope unit="volume">30</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="362">362</biblScope><biblScope unit="page" to="368">368</biblScope></imprint><idno type="ISSN">0022-2011</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2011</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Acmnpv</term><term>Acmnpv loval</term><term>Acmnpv loval antiserum</term><term>Acmnpv polyhedrin</term><term>Antibody titers</term><term>Antiserum</term><term>Assay</term><term>Baculovirus</term><term>Baculoviruses</term><term>Bergold</term><term>Bombyx mori</term><term>Cell culture</term><term>Comparative studies</term><term>Etude comparee</term><term>Granulosis virus</term><term>Immunoradiometric assay</term><term>Inclusion body proteins</term><term>Individual wells</term><term>Insect pathol</term><term>Invertebrate tissue culture</term><term>Krywienczyk</term><term>Loval</term><term>Microtiter</term><term>Microtiter plates</term><term>Microtiter radioimmunoassay</term><term>Microtiter wells</term><term>Nonoccluded virus</term><term>Nuclear polyhedrosis virus</term><term>Nuclear polyhedrosis viruses</term><term>Nucleocapsids</term><term>Optimal conditions</term><term>Pathol</term><term>Phosphate buffer</term><term>Pieris brassicae</term><term>Polyhedrin</term><term>Polyhedrin antisera</term><term>Polyhedrosis</term><term>Preimmune serum</term><term>Radioimmunoassay</term><term>Serological</term><term>Serological characterization</term><term>Serological relationship</term><term>Serological relationships</term><term>Serological studies</term><term>Serum dilution</term><term>Similar experiments</term><term>Sodium azide</term><term>Specific activity</term><term>Specific antibodies</term><term>Viral</term><term>Viral antigens</term><term>Viral proteins</term><term>Volkman</term><term>Znvertebr</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. 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Summers</name><affiliations><json:string>To whom correspondence should be addressed.</json:string><json:string>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</json:string><json:string>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</json:string></affiliations></json:item><json:item><name>Pepper Hoops</name><affiliations><json:string>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</json:string><json:string>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</json:string></affiliations></json:item><json:item><name>Gale E. Smith</name><affiliations><json:string>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</json:string><json:string>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-F4M5KS84-X</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. 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(1973, 1974, 1976)</json:string><json:string>Krywienczyk, 1963</json:string><json:string>Krywienczyk et al., 1958</json:string><json:string>Stephenson et al., 1973</json:string><json:string>Volkman et al. (1976)</json:string><json:string>Tanada and Watanabe, 1971</json:string><json:string>Young et al 1975</json:string><json:string>Scott and Young, 1973</json:string><json:string>Rosenthal et al., 1972</json:string><json:string>Yalow and Berson (1960)</json:string><json:string>Krywienczyk, 1962</json:string><json:string>Young et al. (1975)</json:string><json:string>Summers and Smith (1976)</json:string><json:string>McIntosh and Padhi, 1976</json:string><json:string>Reichelderfer, 1974</json:string><json:string>Croizier and Meynadier, 1972, 1973a, b, 1975</json:string><json:string>Scott et al. (1976)</json:string><json:string>Yalow and Gross, 1976</json:string><json:string>Young and Johnson, 1972</json:string><json:string>Strand and August, 1974</json:string><json:string>Shapiro and Ignoffo, 1970a, b</json:string><json:string>Dalrymple et al., 1972</json:string><json:string>Cardiff et al., 1968</json:string><json:string>Scolnik et al., 1972</json:string><json:string>Krywienczyk and Bergold, 1960a, b, c</json:string><json:string>McIntosh and Maramorosch, 1973</json:string><json:string>Lowry et al. (1951)</json:string><json:string>Greenwood et al.</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-F4M5KS84-X</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - zoology</json:string></wos><scienceMetrix><json:string>1 - natural sciences</json:string><json:string>2 - biology</json:string><json:string>3 - entomology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Agricultural and Biological Sciences</json:string><json:string>3 - Ecology, Evolution, Behavior and Systematics</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences 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USA</affiliation><affiliation>1 Present address: Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Max D.</forename><surname>Summers</surname></persName><affiliation>To whom correspondence should be addressed.</affiliation><affiliation>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><affiliation>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Pepper</forename><surname>Hoops</surname></persName><affiliation>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><affiliation>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Gale E.</forename><surname>Smith</surname></persName><affiliation>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><affiliation>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation></author><idno type="istex">A7DD56A230FA83DA829B5C6B676D7EA7B31CE051</idno><idno type="ark">ark:/67375/6H6-F4M5KS84-X</idno><idno type="DOI">10.1016/0022-2011(77)90146-X</idno><idno type="PII">0022-2011(77)90146-X</idno></analytic><monogr><title level="j">Journal of Invertebrate Pathology</title><title level="j" type="abbrev">YJIPA</title><idno type="pISSN">0022-2011</idno><idno type="PII">S0022-2011(00)X0240-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1977"></date><biblScope unit="volume">30</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="362">362</biblScope><biblScope unit="page" to="368">368</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1977</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. A preliminary study also demonstrated the ability to use this assay to detect specific antiviral antibodies.</p></abstract></profileDesc><revisionDesc><change when="1977">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-F4M5KS84-X/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YJIPA</jid><aid>7790146X</aid><ce:pii>0022-2011(77)90146-X</ce:pii><ce:doi>10.1016/0022-2011(77)90146-X</ce:doi><ce:copyright type="unknown" year="1977"></ce:copyright></item-info><head><ce:title>Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin</ce:title><ce:author-group><ce:author><ce:given-name>Michio</ce:given-name><ce:surname>Ohba</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Max D.</ce:given-name><ce:surname>Summers</ce:surname><ce:cross-ref refid="COR1"><ce:sup>2</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Pepper</ce:given-name><ce:surname>Hoops</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Gale E.</ce:given-name><ce:surname>Smith</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>2</ce:label><ce:text>To whom correspondence should be addressed.</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>Present address: Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="2" month="3" year="1977"></ce:date-received><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A solid-phase microtiter immunoradiometric assay was developed for <ce:italic>Autographa californica</ce:italic> enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. A preliminary study also demonstrated the ability to use this assay to detect specific antiviral antibodies.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Immunoradiometric assay for baculovirus enveloped nucleocapsids and polyhedrin</title></titleInfo><name type="personal"><namePart type="given">Michio</namePart><namePart type="family">Ohba</namePart><affiliation>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><affiliation>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><affiliation>1 Present address: Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka 812, Japan.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Max D.</namePart><namePart type="family">Summers</namePart><affiliation>To whom correspondence should be addressed.</affiliation><affiliation>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><affiliation>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pepper</namePart><namePart type="family">Hoops</namePart><affiliation>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><affiliation>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gale E.</namePart><namePart type="family">Smith</namePart><affiliation>The Cell Research Institute, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><affiliation>The Department of Botany, The University of Texas at Austin, Austin, Texas 78712 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1977</dateIssued><copyrightDate encoding="w3cdtf">1977</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A solid-phase microtiter immunoradiometric assay was developed for Autographa californica enveloped nucleocapsids purified from polyhedra, nonoccluded virus from cell culture, and purified polyhedrin. Comparative studies with antigens and antisera from each source demonstrate that micro-solid-phase immunoradiometric assay was specific within the range of 10–200 ng for the enveloped nucleocapsids and from 200 pg to 200 ng for polyhedrin. A preliminary study also demonstrated the ability to use this assay to detect specific antiviral antibodies.</abstract><relatedItem type="host"><titleInfo><title>Journal of Invertebrate Pathology</title></titleInfo><titleInfo type="abbreviated"><title>YJIPA</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1977</dateIssued></originInfo><identifier type="ISSN">0022-2011</identifier><identifier type="PII">S0022-2011(00)X0240-6</identifier><part><date>1977</date><detail type="volume"><number>30</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>283</start><end>463</end></extent><extent unit="pages"><start>362</start><end>368</end></extent></part></relatedItem><identifier type="istex">A7DD56A230FA83DA829B5C6B676D7EA7B31CE051</identifier><identifier type="ark">ark:/67375/6H6-F4M5KS84-X</identifier><identifier type="DOI">10.1016/0022-2011(77)90146-X</identifier><identifier type="PII">0022-2011(77)90146-X</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-F4M5KS84-X/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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