A quantitative system for the study of collagen and hyaluronic acid synthesis in vitro
Identifieur interne : 001752 ( Istex/Corpus ); précédent : 001751; suivant : 001753A quantitative system for the study of collagen and hyaluronic acid synthesis in vitro
Auteurs : Stanley L. Wiener ; Myra Mass ; Morton Urivetzky ; Edward MeilmanSource :
- BBA Section Nucleic Acids And Protein Synthesis [ 0005-2787 ] ; 1968.
English descriptors
- Teeft :
- Acid, Aliquot, Amino, Amino acid incorporation, Amino acid mixture, Biol, Biosynthesis, Bone cells, Chem, Citrate, Collagen, Collagen biosynthesis, Connective, Connective tissue, Connective tissue protein synthesis, Crude homogenate, Enzymatic analysis, Ethanol, Flask, Fractional distribution, Glucose, Homogenate, Hyaluronic acid synthesis, Incorporation, Incorporation rate, Incorporation rates, Incubation, Individual fractions, Isotope, Isotope addition, Leech, Linear relationship, Nac1 extracts, Naci, Pancreatic elastase, Procollagen synthesis, Pronase lysis, Protein content, Protein synthesis, Rabbit skin, Radioactivity, Small amounts, Specific activity, Standard deviations, Steroid hormones, Substrate requirements, Tissue aliquot, Total incorporation, Trichloroacetic, Trichloroacetic acid, Trichloroacetie acid, Triehloroacetie acid, Uantitative system, Variable amount, Various tissue parameters.
Abstract
Abstract: The relationship of various parameters to the biosynthesis of connective tissue proteins and polysaccharides in rabbit sponge capsular tissue in vitro is presented.A linear relationship of isotope incorporation to microsomal RNA and DNA content per flask was found. Enzymatic analysis of 1 M NaCl-soluble products indicates that the [14C]proline-rich macromolecule is at least 50% procollagen and d-[I-14C]glucosamine-containing polymer is 60–70% hyaluronate and 28% glycoprotein.[14C]Tyrosine was found to be rapidly incorporated into 1 M NaCl-soluble, collagenase-resistant proteins.Applications of these methods to the study of the connective tissue requirements for glucose and other simple metabolites is presented. The effects of alcohol, steroid hormones and insulin on connective tissue synthesis is also included.
Url:
DOI: 10.1016/0005-2787(68)90506-6
Links to Exploration step
ISTEX:A4A346CDBFE3F0DA46E1697E0CC894154E7EFFD1Le document en format XML
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U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Mass, Myra" sort="Mass, Myra" uniqKey="Mass M" first="Myra" last="Mass">Myra Mass</name><affiliation><mods:affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Urivetzky, Morton" sort="Urivetzky, Morton" uniqKey="Urivetzky M" first="Morton" last="Urivetzky">Morton Urivetzky</name><affiliation><mods:affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Meilman, Edward" sort="Meilman, Edward" uniqKey="Meilman E" first="Edward" last="Meilman">Edward Meilman</name><affiliation><mods:affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. 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Enzymatic analysis of 1 M NaCl-soluble products indicates that the [14C]proline-rich macromolecule is at least 50% procollagen and d-[I-14C]glucosamine-containing polymer is 60–70% hyaluronate and 28% glycoprotein.[14C]Tyrosine was found to be rapidly incorporated into 1 M NaCl-soluble, collagenase-resistant proteins.Applications of these methods to the study of the connective tissue requirements for glucose and other simple metabolites is presented. The effects of alcohol, steroid hormones and insulin on connective tissue synthesis is also included.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>glucose</json:string><json:string>biol</json:string><json:string>citrate</json:string><json:string>isotope</json:string><json:string>aliquot</json:string><json:string>biosynthesis</json:string><json:string>amino acid incorporation</json:string><json:string>naci</json:string><json:string>leech</json:string><json:string>chem</json:string><json:string>incorporation</json:string><json:string>specific activity</json:string><json:string>amino</json:string><json:string>protein synthesis</json:string><json:string>total incorporation</json:string><json:string>trichloroacetic</json:string><json:string>ethanol</json:string><json:string>collagen biosynthesis</json:string><json:string>connective tissue</json:string><json:string>steroid hormones</json:string><json:string>incubation</json:string><json:string>collagen</json:string><json:string>flask</json:string><json:string>protein content</json:string><json:string>linear relationship</json:string><json:string>substrate requirements</json:string><json:string>tissue aliquot</json:string><json:string>hyaluronic acid synthesis</json:string><json:string>trichloroacetic acid</json:string><json:string>crude homogenate</json:string><json:string>trichloroacetie acid</json:string><json:string>small amounts</json:string><json:string>various tissue parameters</json:string><json:string>bone cells</json:string><json:string>connective tissue protein synthesis</json:string><json:string>procollagen synthesis</json:string><json:string>individual fractions</json:string><json:string>incorporation rate</json:string><json:string>variable amount</json:string><json:string>triehloroacetie acid</json:string><json:string>nac1 extracts</json:string><json:string>fractional distribution</json:string><json:string>uantitative system</json:string><json:string>pancreatic elastase</json:string><json:string>amino acid mixture</json:string><json:string>pronase lysis</json:string><json:string>isotope addition</json:string><json:string>standard deviations</json:string><json:string>enzymatic analysis</json:string><json:string>incorporation rates</json:string><json:string>rabbit skin</json:string><json:string>homogenate</json:string><json:string>acid</json:string><json:string>radioactivity</json:string><json:string>connective</json:string></teeft></keywords><author><json:item><name>Stanley L Wiener</name><affiliations><json:string>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. 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U.S.A.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Myra</forename><surname>Mass</surname></persName><affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Morton</forename><surname>Urivetzky</surname></persName><affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Edward</forename><surname>Meilman</surname></persName><affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</affiliation></author><idno type="istex">A4A346CDBFE3F0DA46E1697E0CC894154E7EFFD1</idno><idno type="ark">ark:/67375/6H6-0LGL12WK-9</idno><idno type="DOI">10.1016/0005-2787(68)90506-6</idno><idno type="PII">0005-2787(68)90506-6</idno></analytic><monogr><title level="j">BBA Section Nucleic Acids And Protein Synthesis</title><title level="j" type="abbrev">BBANAP</title><idno type="pISSN">0005-2787</idno><idno type="PII">S0005-2787(00)X0289-4</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1968"></date><biblScope unit="volume">166</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="229">229</biblScope><biblScope unit="page" to="241">241</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1968</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The relationship of various parameters to the biosynthesis of connective tissue proteins and polysaccharides in rabbit sponge capsular tissue in vitro is presented.A linear relationship of isotope incorporation to microsomal RNA and DNA content per flask was found. Enzymatic analysis of 1 M NaCl-soluble products indicates that the [14C]proline-rich macromolecule is at least 50% procollagen and d-[I-14C]glucosamine-containing polymer is 60–70% hyaluronate and 28% glycoprotein.[14C]Tyrosine was found to be rapidly incorporated into 1 M NaCl-soluble, collagenase-resistant proteins.Applications of these methods to the study of the connective tissue requirements for glucose and other simple metabolites is presented. The effects of alcohol, steroid hormones and insulin on connective tissue synthesis is also included.</p></abstract></profileDesc><revisionDesc><change when="1968">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-0LGL12WK-9/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>BBANAP</jid><aid>68905066</aid><ce:pii>0005-2787(68)90506-6</ce:pii><ce:doi>10.1016/0005-2787(68)90506-6</ce:doi><ce:copyright type="unknown" year="1968"></ce:copyright></item-info><head><ce:title>A quantitative system for the study of collagen and hyaluronic acid synthesis <ce:italic>in vitro</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>Stanley L</ce:given-name><ce:surname>Wiener</ce:surname></ce:author><ce:author><ce:given-name>Myra</ce:given-name><ce:surname>Mass</ce:surname></ce:author><ce:author><ce:given-name>Morton</ce:given-name><ce:surname>Urivetzky</ce:surname></ce:author><ce:author><ce:given-name>Edward</ce:given-name><ce:surname>Meilman</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="5" month="2" year="1968"></ce:date-received><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The relationship of various parameters to the biosynthesis of connective tissue proteins and polysaccharides in rabbit sponge capsular tissue in <ce:italic>vitro</ce:italic> is presented.</ce:simple-para><ce:simple-para>A linear relationship of isotope incorporation to microsomal RNA and DNA content per flask was found. Enzymatic analysis of 1 M NaCl-soluble products indicates that the [<ce:sup loc="pre">14</ce:sup>C]proline-rich macromolecule is at least 50% procollagen and <ce:small-caps>d</ce:small-caps>-[I-<ce:sup>14</ce:sup>C]glucosamine-containing polymer is 60–70% hyaluronate and 28% glycoprotein.</ce:simple-para><ce:simple-para>[<ce:sup loc="pre">14</ce:sup>C]Tyrosine was found to be rapidly incorporated into 1 M NaCl-soluble, collagenase-resistant proteins.</ce:simple-para><ce:simple-para>Applications of these methods to the study of the connective tissue requirements for glucose and other simple metabolites is presented. The effects of alcohol, steroid hormones and insulin on connective tissue synthesis is also included.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>A quantitative system for the study of collagen and hyaluronic acid synthesis in vitro</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>A quantitative system for the study of collagen and hyaluronic acid synthesis</title></titleInfo><name type="personal"><namePart type="given">Stanley L</namePart><namePart type="family">Wiener</namePart><affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Myra</namePart><namePart type="family">Mass</namePart><affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Morton</namePart><namePart type="family">Urivetzky</namePart><affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Edward</namePart><namePart type="family">Meilman</namePart><affiliation>Department of Medicine, Long Island Jewish Hospital, New Hyde Park, N.Y. U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1968</dateIssued><copyrightDate encoding="w3cdtf">1968</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The relationship of various parameters to the biosynthesis of connective tissue proteins and polysaccharides in rabbit sponge capsular tissue in vitro is presented.A linear relationship of isotope incorporation to microsomal RNA and DNA content per flask was found. Enzymatic analysis of 1 M NaCl-soluble products indicates that the [14C]proline-rich macromolecule is at least 50% procollagen and d-[I-14C]glucosamine-containing polymer is 60–70% hyaluronate and 28% glycoprotein.[14C]Tyrosine was found to be rapidly incorporated into 1 M NaCl-soluble, collagenase-resistant proteins.Applications of these methods to the study of the connective tissue requirements for glucose and other simple metabolites is presented. 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