Interaction between acridine orange and polyriboadenylic acid
Identifieur interne : 001717 ( Istex/Corpus ); précédent : 001716; suivant : 001718Interaction between acridine orange and polyriboadenylic acid
Auteurs : Toyoko Imae ; Shoji Hayashi ; Shoichi Ikeda ; Tomohiko SakakiSource :
- International Journal of Biological Macromolecules [ 0141-8130 ] ; 1981.
English descriptors
- Teeft :
- Absorption band, Absorption spectra, Acridine, Acridine interactions, Acridine mixtures, Adenine bases, Antiparallel, Antiparallel type, Aqueous solution, August, Base pairs, Binding process, Biol, Biopolymers, Chem, Circular dichroic bands, Circular dichroism, Conservative pair, Dichroic, Dichroic band, Dichroic bands, Dichroism, Dimer, Dimeric, Dimeric molecules, Equilibrium dialysis, External association, Free acridine, Helical, Helical content, Helical parts, Helix, Imae, Inner compartment, Intercalated, Intercalated monomer, Intercalated monomers, Intercalation, Ionic strength, Isosbestic point, Macromol, Molar, Molar ellipticity, Molar extinction coefficient, Molecule, Monomer, Monomeric, Naci, Negative band, Optical activity, Positive band, Residue extinction coefficient, Toyoko, Toyoko imae, Toyoko lmae, Visible absorption spectra, Visking membrane.
Abstract
Abstract: The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.
Url:
DOI: 10.1016/0141-8130(81)90037-4
Links to Exploration step
ISTEX:55DFF1325A2ADF1F41E48592405B7A9A556F4D6CLe document en format XML
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sort="Sakaki, Tomohiko" uniqKey="Sakaki T" first="Tomohiko" last="Sakaki">Tomohiko Sakaki</name><affiliation><mods:affiliation>Department of Chemistry, College of General Education, Nagoya University Chikusa, Nagoya, Japan</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:55DFF1325A2ADF1F41E48592405B7A9A556F4D6C</idno><date when="1981" year="1981">1981</date><idno type="doi">10.1016/0141-8130(81)90037-4</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-2CM385D5-X/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001717</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001717</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Interaction between acridine orange and polyriboadenylic acid</title><author><name sortKey="Imae, Toyoko" sort="Imae, Toyoko" uniqKey="Imae T" first="Toyoko" last="Imae">Toyoko 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pairs</term><term>Binding process</term><term>Biol</term><term>Biopolymers</term><term>Chem</term><term>Circular dichroic bands</term><term>Circular dichroism</term><term>Conservative pair</term><term>Dichroic</term><term>Dichroic band</term><term>Dichroic bands</term><term>Dichroism</term><term>Dimer</term><term>Dimeric</term><term>Dimeric molecules</term><term>Equilibrium dialysis</term><term>External association</term><term>Free acridine</term><term>Helical</term><term>Helical content</term><term>Helical parts</term><term>Helix</term><term>Imae</term><term>Inner compartment</term><term>Intercalated</term><term>Intercalated monomer</term><term>Intercalated monomers</term><term>Intercalation</term><term>Ionic strength</term><term>Isosbestic point</term><term>Macromol</term><term>Molar</term><term>Molar ellipticity</term><term>Molar extinction coefficient</term><term>Molecule</term><term>Monomer</term><term>Monomeric</term><term>Naci</term><term>Negative band</term><term>Optical activity</term><term>Positive band</term><term>Residue extinction coefficient</term><term>Toyoko</term><term>Toyoko imae</term><term>Toyoko lmae</term><term>Visible absorption spectra</term><term>Visking membrane</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>acridine</json:string><json:string>dichroism</json:string><json:string>intercalated</json:string><json:string>dichroic</json:string><json:string>circular dichroism</json:string><json:string>dimer</json:string><json:string>dimeric</json:string><json:string>helical</json:string><json:string>biol</json:string><json:string>intercalation</json:string><json:string>absorption band</json:string><json:string>biopolymers</json:string><json:string>helix</json:string><json:string>monomeric</json:string><json:string>intercalated monomers</json:string><json:string>chem</json:string><json:string>circular dichroic bands</json:string><json:string>absorption spectra</json:string><json:string>august</json:string><json:string>naci</json:string><json:string>toyoko</json:string><json:string>imae</json:string><json:string>antiparallel</json:string><json:string>macromol</json:string><json:string>molar</json:string><json:string>external association</json:string><json:string>acridine interactions</json:string><json:string>helical content</json:string><json:string>positive band</json:string><json:string>negative band</json:string><json:string>toyoko imae</json:string><json:string>binding process</json:string><json:string>helical parts</json:string><json:string>isosbestic point</json:string><json:string>dimeric molecules</json:string><json:string>dichroic band</json:string><json:string>antiparallel type</json:string><json:string>monomer</json:string><json:string>residue extinction coefficient</json:string><json:string>visible absorption spectra</json:string><json:string>molar ellipticity</json:string><json:string>free acridine</json:string><json:string>toyoko lmae</json:string><json:string>acridine mixtures</json:string><json:string>conservative pair</json:string><json:string>ionic strength</json:string><json:string>aqueous solution</json:string><json:string>visking membrane</json:string><json:string>dichroic bands</json:string><json:string>molar extinction coefficient</json:string><json:string>equilibrium dialysis</json:string><json:string>optical activity</json:string><json:string>base pairs</json:string><json:string>adenine bases</json:string><json:string>intercalated monomer</json:string><json:string>inner compartment</json:string><json:string>molecule</json:string></teeft></keywords><author><json:item><name>Toyoko Imae</name><affiliations><json:string>Department of Chemistry, Faculty of Science, Nagoya University, Japan</json:string></affiliations></json:item><json:item><name>Shoji Hayashi</name><affiliations><json:string>Department of Chemistry, Faculty of Science, Nagoya University, Japan</json:string></affiliations></json:item><json:item><name>Shoichi Ikeda</name><affiliations><json:string>Department of Chemistry, Faculty of Science, Nagoya University, Japan</json:string></affiliations></json:item><json:item><name>Tomohiko Sakaki</name><affiliations><json:string>Department of Chemistry, College of General Education, Nagoya University Chikusa, Nagoya, Japan</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-2CM385D5-X</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</abstract><qualityIndicators><score>9.34</score><pdfWordCount>5220</pdfWordCount><pdfCharCount>27621</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>591 x 843 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>195</abstractWordCount><abstractCharCount>1317</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Interaction between acridine orange and polyriboadenylic acid</title><pii><json:string>0141-8130(81)90037-4</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>International Journal of Biological Macromolecules</title><language><json:string>unknown</json:string></language><publicationDate>1981</publicationDate><issn><json:string>0141-8130</json:string></issn><pii><json:string>S0141-8130(00)X0091-8</json:string></pii><volume>3</volume><issue>4</issue><pages><first>259</first><last>266</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1981</json:string></date><geogName></geogName><orgName><json:string>Nagoya University</json:string><json:string>L Biochemicals Inc.</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>J. 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The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</p></abstract></profileDesc><revisionDesc><change when="1981">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-2CM385D5-X/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>BIOMAC</jid><aid>81900374</aid><ce:pii>0141-8130(81)90037-4</ce:pii><ce:doi>10.1016/0141-8130(81)90037-4</ce:doi><ce:copyright type="unknown" year="1981"></ce:copyright></item-info><head><ce:title>Interaction between acridine orange and polyriboadenylic acid</ce:title><ce:author-group><ce:author><ce:given-name>Toyoko</ce:given-name><ce:surname>Imae</ce:surname></ce:author><ce:author><ce:given-name>Shoji</ce:given-name><ce:surname>Hayashi</ce:surname></ce:author><ce:author><ce:given-name>Shoichi</ce:given-name><ce:surname>Ikeda</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Chemistry, Faculty of Science, Nagoya University, Japan</ce:textfn></ce:affiliation></ce:author-group><ce:author-group><ce:author><ce:given-name>Tomohiko</ce:given-name><ce:surname>Sakaki</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Chemistry, College of General Education, Nagoya University Chikusa, Nagoya, Japan</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="27" month="5" year="1980"></ce:date-received><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Interaction between acridine orange and polyriboadenylic acid</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Interaction between acridine orange and polyriboadenylic acid</title></titleInfo><name type="personal"><namePart type="given">Toyoko</namePart><namePart type="family">Imae</namePart><affiliation>Department of Chemistry, Faculty of Science, Nagoya University, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shoji</namePart><namePart type="family">Hayashi</namePart><affiliation>Department of Chemistry, Faculty of Science, Nagoya University, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shoichi</namePart><namePart type="family">Ikeda</namePart><affiliation>Department of Chemistry, Faculty of Science, Nagoya University, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tomohiko</namePart><namePart type="family">Sakaki</namePart><affiliation>Department of Chemistry, College of General Education, Nagoya University Chikusa, Nagoya, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1981</dateIssued><copyrightDate encoding="w3cdtf">1981</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</abstract><relatedItem type="host"><titleInfo><title>International Journal of Biological Macromolecules</title></titleInfo><titleInfo type="abbreviated"><title>BIOMAC</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1981</dateIssued></originInfo><identifier type="ISSN">0141-8130</identifier><identifier type="PII">S0141-8130(00)X0091-8</identifier><part><date>1981</date><detail type="volume"><number>3</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="issue-pages"><start>217</start><end>280</end></extent><extent unit="pages"><start>259</start><end>266</end></extent></part></relatedItem><identifier type="istex">55DFF1325A2ADF1F41E48592405B7A9A556F4D6C</identifier><identifier type="ark">ark:/67375/6H6-2CM385D5-X</identifier><identifier type="DOI">10.1016/0141-8130(81)90037-4</identifier><identifier type="PII">0141-8130(81)90037-4</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-2CM385D5-X/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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