Interaction between acridine orange and polyriboadenylic acid
Identifieur interne : 001717 ( Istex/Corpus ); précédent : 001716; suivant : 001718Interaction between acridine orange and polyriboadenylic acid
Auteurs : Toyoko Imae ; Shoji Hayashi ; Shoichi Ikeda ; Tomohiko SakakiSource :
- International Journal of Biological Macromolecules [ 0141-8130 ] ; 1981.
English descriptors
- Teeft :
- Absorption band, Absorption spectra, Acridine, Acridine interactions, Acridine mixtures, Adenine bases, Antiparallel, Antiparallel type, Aqueous solution, August, Base pairs, Binding process, Biol, Biopolymers, Chem, Circular dichroic bands, Circular dichroism, Conservative pair, Dichroic, Dichroic band, Dichroic bands, Dichroism, Dimer, Dimeric, Dimeric molecules, Equilibrium dialysis, External association, Free acridine, Helical, Helical content, Helical parts, Helix, Imae, Inner compartment, Intercalated, Intercalated monomer, Intercalated monomers, Intercalation, Ionic strength, Isosbestic point, Macromol, Molar, Molar ellipticity, Molar extinction coefficient, Molecule, Monomer, Monomeric, Naci, Negative band, Optical activity, Positive band, Residue extinction coefficient, Toyoko, Toyoko imae, Toyoko lmae, Visible absorption spectra, Visking membrane.
Abstract
Abstract: The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.
Url:
DOI: 10.1016/0141-8130(81)90037-4
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</div>
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<abstract>Abstract: The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</abstract>
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<ce:abstract-sec><ce:simple-para>The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</ce:simple-para>
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<name type="personal"><namePart type="given">Toyoko</namePart>
<namePart type="family">Imae</namePart>
<affiliation>Department of Chemistry, Faculty of Science, Nagoya University, Japan</affiliation>
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<name type="personal"><namePart type="given">Shoji</namePart>
<namePart type="family">Hayashi</namePart>
<affiliation>Department of Chemistry, Faculty of Science, Nagoya University, Japan</affiliation>
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<name type="personal"><namePart type="given">Shoichi</namePart>
<namePart type="family">Ikeda</namePart>
<affiliation>Department of Chemistry, Faculty of Science, Nagoya University, Japan</affiliation>
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<name type="personal"><namePart type="given">Tomohiko</namePart>
<namePart type="family">Sakaki</namePart>
<affiliation>Department of Chemistry, College of General Education, Nagoya University Chikusa, Nagoya, Japan</affiliation>
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<abstract lang="en">Abstract: The absorption spectra and circular dichroism of aqueous solutions of acridine orange mixed with polY(riboadenylic acid) [poly(rA)] have been measured for different mixing ratios at acid and neutral pH. The binding ratio of dye to poly(rA) has been determined by equilibrium dialysis. At acid pH where poly(rA) is in a double-stranded helix, monomeric dye molecules are intercalated between base pairs, first sparsely and then at neighbouring sites with mutual coupling, as the nucleotide-to-dye mixing ratio decreases. In the presence of excess dye, dimeric dye molecules of antiparallel type are bound to phosphate groups electrostatically and stack together to form linear sequences along a poly(rA) chain. At neutral pH where poly(rA) is single-stranded, isolated intercalation of monomeric dye molecules can occur in the helical parts. At intermediate mixing ratios, half-intercalated dimeric dye molecules are bound to adjacent sites and electronically coupled, inducing characteristic circular dichroism. In the presence of higher amounts of dye, external stacking of dimeric dye molecules of antiparallel type occurs along a poly(rA) chain. The binding of dye cations is suppressed to some degree at acid pH compared to that at neutral pH, owing to the repulsion exerted by protonated adenine bases.</abstract>
<relatedItem type="host"><titleInfo><title>International Journal of Biological Macromolecules</title>
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<identifier type="ISSN">0141-8130</identifier>
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