The homeobox gene MEIS1 is amplified in IMR-32 and highly expressed in other neuroblastoma cell lines
Identifieur interne : 001581 ( Istex/Corpus ); précédent : 001580; suivant : 001582The homeobox gene MEIS1 is amplified in IMR-32 and highly expressed in other neuroblastoma cell lines
Auteurs : T. A. Jones ; R. H. Flomen ; G. Senger ; D. Nižeti ; D. SheerSource :
- European Journal of Cancer [ 0959-8049 ] ; 2000.
English descriptors
- Teeft :
- Cell line, Cell lines, Chromosome, Chromosome band, Clone, Contig, Ddx1, Erythroleukaemia cell line, European journal, Gene, Genomic, Genomic clone, Hgmp resource centre, High expression, High levels, Homeobox, Homeobox gene, Homeobox genes, Hybridisation, Leukaemia, Life technologies, Medulloblastoma, Medulloblastoma cell lines, Meis1, Meis1 expression, Meis1 gene, Mrna, Mycn, Myeloid, Myeloid ecotropic integration site, Neuroblastoma, Neuroblastoma cell line, Neuroblastoma cell lines, Other neuroblastoma, Pac, Positive clones, Tumour.
Abstract
Abstract: Neuroblastoma is a childhood tumour of the sympathetic nervous system that demonstrates striking clinical heterogeneity. In order to determine which genes are abnormally expressed in neuroblastoma, we screened regions of amplification from the short arm of chromosome 2 in the neuroblastoma cell line IMR-32 and found that the homeobox gene, myeloid ecotropic integration site 1 (MEIS1), is highly amplified. MEIS1 normally maps to chromosome band 2p14. High expression of MEIS1 without amplification was also found in other neuroblastoma cell lines, with and without MYCN amplification, and in medulloblastoma and erythroleukaemia cell lines. MEIS1 is highly expressed in cerebellum and ubiquitously expressed in normal immunohaematopoietic tissues and is thought to be important in cell proliferation and differentiation. While several lines of evidence point towards a role for homeobox genes in the development of other malignancies, this is the first report showing the amplification of a homeobox gene in neuroblastoma.
Url:
DOI: 10.1016/S0959-8049(00)00332-4
Links to Exploration step
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<front><div type="abstract" xml:lang="en">Abstract: Neuroblastoma is a childhood tumour of the sympathetic nervous system that demonstrates striking clinical heterogeneity. In order to determine which genes are abnormally expressed in neuroblastoma, we screened regions of amplification from the short arm of chromosome 2 in the neuroblastoma cell line IMR-32 and found that the homeobox gene, myeloid ecotropic integration site 1 (MEIS1), is highly amplified. MEIS1 normally maps to chromosome band 2p14. High expression of MEIS1 without amplification was also found in other neuroblastoma cell lines, with and without MYCN amplification, and in medulloblastoma and erythroleukaemia cell lines. MEIS1 is highly expressed in cerebellum and ubiquitously expressed in normal immunohaematopoietic tissues and is thought to be important in cell proliferation and differentiation. While several lines of evidence point towards a role for homeobox genes in the development of other malignancies, this is the first report showing the amplification of a homeobox gene in neuroblastoma.</div>
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<note type="content">Fig. 1: Amplification of: (a) MYCN (pNb-1); and (c) myeloid ecotropic integration site 1 (MEIS1) (I.M.A.G.E. clone 223490) along the p arm of the derivative chromosome 1 in the cell line IMR-32. Biotinylated probes were detected with avidin–fluoresceinisothiocyanate (FITC). Fluorescence in situ hybridisation (FISH) of DDX1 (lambda 2001) onto IMR-32 also showed amplification (data not shown). (b) Two-colour FISH to normal human chromosome 2 showing the order: telomere — group II (PAC 172B3) (Texas Red) — group I (PAC 2E20) (FITC) — centromere.</note>
<note type="content">Fig. 2: PAC contig. The scale in kb is shown at the top of the figure, with the positions of sequence tagged site (STS) markers and centromere/telomere orientation indicated above. The open bar below is a representation of genomic DNA showing the positions of XhoI (X), SalI (S) and NotI (N) restriction enzyme sites. Sizes of restriction fragments are shown by vertical lines within the open bar. The location of the MEIS1 gene is indicated directly below the open bar and genomic fragments containing gene coding sequences are marked with horizontal bars. The arrow indicates the 5′ to 3′ orientation of the gene. Brackets beneath the open bar show that the order of these XhoI sites has not been determined. The lower part of the figure comprises the PAC contig, with SP6/T7 vector ends boxed and restriction enzyme sites denoted by vertical lines attached to the PACs. The positions of the original two groups of clones are marked beneath the PAC contig.</note>
<note type="content">Fig. 3: Reverse transcriptase-polymerase chain reaction (RT-PCR) showing myeloid ecotropic integration site 1 (MEIS1) expression. Ubiquitous expression is shown in normal cerebellum, bone marrow and brain, the neuroblastoma cell lines (IMR-32, SK-N-AS, SK-N-FI, Kelly, SK-N-LI, SK-N-Be, SK-N-DZ and CHP212), the medulloblastoma cell lines (HTB186, TE671 and D283), an erythroleukaemia cell line (HEL 92.1.7), a chronic myeloid leukaemia cell line (K-562), a follicular thyroid carcinoma cell line (RO82-W-1) and a human lung carcinoma cell line (A549). No expression was found in the human Burkitt's lymphoma cell line (Daudi). Controls lacking cDNA (shown) and controls lacking reverse transcriptase (data not shown) were negative for all samples.</note>
<note type="content">Fig. 4: Northern blot analysis of myeloid ecotropic integration site 1 (MEIS1) expression in the MYCN-amplified neuroblastoma cell lines CHP212, SK-N-DZ, SK-N-Be and IMR-32 and the neuroblastoma cell line that lacks amplification, SK-N-AS. 2 μg Poly (A)+ RNA were loaded in each lane and the blot sequentially hybridised with the MEIS1 I.M.A.G.E. clone 223490, MYCN (pNb-1) and β-actin.</note>
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<abstract xml:lang="en"><p>Abstract: Neuroblastoma is a childhood tumour of the sympathetic nervous system that demonstrates striking clinical heterogeneity. In order to determine which genes are abnormally expressed in neuroblastoma, we screened regions of amplification from the short arm of chromosome 2 in the neuroblastoma cell line IMR-32 and found that the homeobox gene, myeloid ecotropic integration site 1 (MEIS1), is highly amplified. MEIS1 normally maps to chromosome band 2p14. High expression of MEIS1 without amplification was also found in other neuroblastoma cell lines, with and without MYCN amplification, and in medulloblastoma and erythroleukaemia cell lines. MEIS1 is highly expressed in cerebellum and ubiquitously expressed in normal immunohaematopoietic tissues and is thought to be important in cell proliferation and differentiation. While several lines of evidence point towards a role for homeobox genes in the development of other malignancies, this is the first report showing the amplification of a homeobox gene in neuroblastoma.</p>
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<abstract lang="en">Abstract: Neuroblastoma is a childhood tumour of the sympathetic nervous system that demonstrates striking clinical heterogeneity. In order to determine which genes are abnormally expressed in neuroblastoma, we screened regions of amplification from the short arm of chromosome 2 in the neuroblastoma cell line IMR-32 and found that the homeobox gene, myeloid ecotropic integration site 1 (MEIS1), is highly amplified. MEIS1 normally maps to chromosome band 2p14. High expression of MEIS1 without amplification was also found in other neuroblastoma cell lines, with and without MYCN amplification, and in medulloblastoma and erythroleukaemia cell lines. MEIS1 is highly expressed in cerebellum and ubiquitously expressed in normal immunohaematopoietic tissues and is thought to be important in cell proliferation and differentiation. While several lines of evidence point towards a role for homeobox genes in the development of other malignancies, this is the first report showing the amplification of a homeobox gene in neuroblastoma.</abstract>
<note type="content">Fig. 1: Amplification of: (a) MYCN (pNb-1); and (c) myeloid ecotropic integration site 1 (MEIS1) (I.M.A.G.E. clone 223490) along the p arm of the derivative chromosome 1 in the cell line IMR-32. Biotinylated probes were detected with avidin–fluoresceinisothiocyanate (FITC). Fluorescence in situ hybridisation (FISH) of DDX1 (lambda 2001) onto IMR-32 also showed amplification (data not shown). (b) Two-colour FISH to normal human chromosome 2 showing the order: telomere — group II (PAC 172B3) (Texas Red) — group I (PAC 2E20) (FITC) — centromere.</note>
<note type="content">Fig. 2: PAC contig. The scale in kb is shown at the top of the figure, with the positions of sequence tagged site (STS) markers and centromere/telomere orientation indicated above. The open bar below is a representation of genomic DNA showing the positions of XhoI (X), SalI (S) and NotI (N) restriction enzyme sites. Sizes of restriction fragments are shown by vertical lines within the open bar. The location of the MEIS1 gene is indicated directly below the open bar and genomic fragments containing gene coding sequences are marked with horizontal bars. The arrow indicates the 5′ to 3′ orientation of the gene. Brackets beneath the open bar show that the order of these XhoI sites has not been determined. The lower part of the figure comprises the PAC contig, with SP6/T7 vector ends boxed and restriction enzyme sites denoted by vertical lines attached to the PACs. The positions of the original two groups of clones are marked beneath the PAC contig.</note>
<note type="content">Fig. 3: Reverse transcriptase-polymerase chain reaction (RT-PCR) showing myeloid ecotropic integration site 1 (MEIS1) expression. Ubiquitous expression is shown in normal cerebellum, bone marrow and brain, the neuroblastoma cell lines (IMR-32, SK-N-AS, SK-N-FI, Kelly, SK-N-LI, SK-N-Be, SK-N-DZ and CHP212), the medulloblastoma cell lines (HTB186, TE671 and D283), an erythroleukaemia cell line (HEL 92.1.7), a chronic myeloid leukaemia cell line (K-562), a follicular thyroid carcinoma cell line (RO82-W-1) and a human lung carcinoma cell line (A549). No expression was found in the human Burkitt's lymphoma cell line (Daudi). Controls lacking cDNA (shown) and controls lacking reverse transcriptase (data not shown) were negative for all samples.</note>
<note type="content">Fig. 4: Northern blot analysis of myeloid ecotropic integration site 1 (MEIS1) expression in the MYCN-amplified neuroblastoma cell lines CHP212, SK-N-DZ, SK-N-Be and IMR-32 and the neuroblastoma cell line that lacks amplification, SK-N-AS. 2 μg Poly (A)+ RNA were loaded in each lane and the blot sequentially hybridised with the MEIS1 I.M.A.G.E. clone 223490, MYCN (pNb-1) and β-actin.</note>
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