Analysis of baculovirus genomes with restriction endonucleases
Identifieur interne : 001576 ( Istex/Corpus ); précédent : 001575; suivant : 001577Analysis of baculovirus genomes with restriction endonucleases
Auteurs : Gale E. Smith ; Max D. SummersSource :
- Virology [ 0042-6822 ] ; 1978.
English descriptors
- Teeft :
- Acmnpv, Agarose, Agarose electrophoresis, Baculovirus, Baculovirus dnas, Baculoviruses, Cell culture, Cell protein, Digestion, Dna, Electrophoresed, Endonuclease, Extracellular, Extracellular virus, Fragment, Genome, Genomic, Genomic heterogeneity, Infectious forms, Mnpv, Molar, Molecular weight, Molecular weights, Plaque, Polyacrylamide, Polyhedron, Polyhedrosis, Polypeptide, Restriction, Restriction endonuclease, Restriction enzymes, Restriction fragments, Restriction pattern, Restriction patterns, Singles, Structural polypeptides, Submolar, Submolar fragments, Viral, Viral dnas, Virus, Virus dnas, Volkman.
Abstract
Abstract: The viral DNAs from nine wild-type insect baculoviruses have been isolated and the EcoR-1 restriction endonuclease fragment patterns compared. Genomic heterogeneity could be detected in the DNA restriction patterns of four of these wild-type baculoviruses. Three infectious virus forms (two that are occluded in the nucleus and an extracellular virus that has budded from the plasma membrane of infected cells) of a nuclear polyhedrosis virus with multiple nucleocapsids per envelope (MNPV) from the lepidopteran insect, Autographa californica are shown to be phenotypically distinct by comparison of viral structural polypeptides by polyacrylamide gel electrophoresis and autoradiography of L[35S]methio-nine-labeled virus proteins. The three phenotypic forms were cloned by successive plaque purification and eight distinct variants were identified from 11 plaque-purified viruses by genotypec analysis with EcoR-1 and HindIII restriction endonuclease. Isolation of variants from the three phenotypic forms has shown that each of the infectious forms is heterogeneous and that no segregation of genotypes among the three forms was evident. The characteristic restriction fragment patterns of several variants were maintained upon multiple passage in cell culture.
Url:
DOI: 10.1016/0042-6822(78)90193-9
Links to Exploration step
ISTEX:C45FF837DC155C02E996F19DB2AA1E5D21C421D5Le document en format XML
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Smith</name><affiliation><mods:affiliation>Department of Entomology, Texas A & M University, College Station, Texas 77843, USA</mods:affiliation></affiliation></author><author><name sortKey="Summers, Max D" sort="Summers, Max D" uniqKey="Summers M" first="Max D." last="Summers">Max D. Summers</name><affiliation><mods:affiliation>Department of Entomology, Texas A & M University, College Station, Texas 77843, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1978">1978</date><biblScope unit="volume">89</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="517">517</biblScope><biblScope unit="page" to="527">527</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acmnpv</term><term>Agarose</term><term>Agarose electrophoresis</term><term>Baculovirus</term><term>Baculovirus dnas</term><term>Baculoviruses</term><term>Cell culture</term><term>Cell protein</term><term>Digestion</term><term>Dna</term><term>Electrophoresed</term><term>Endonuclease</term><term>Extracellular</term><term>Extracellular virus</term><term>Fragment</term><term>Genome</term><term>Genomic</term><term>Genomic heterogeneity</term><term>Infectious forms</term><term>Mnpv</term><term>Molar</term><term>Molecular weight</term><term>Molecular weights</term><term>Plaque</term><term>Polyacrylamide</term><term>Polyhedron</term><term>Polyhedrosis</term><term>Polypeptide</term><term>Restriction</term><term>Restriction endonuclease</term><term>Restriction enzymes</term><term>Restriction fragments</term><term>Restriction pattern</term><term>Restriction patterns</term><term>Singles</term><term>Structural polypeptides</term><term>Submolar</term><term>Submolar fragments</term><term>Viral</term><term>Viral dnas</term><term>Virus</term><term>Virus dnas</term><term>Volkman</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The viral DNAs from nine wild-type insect baculoviruses have been isolated and the EcoR-1 restriction endonuclease fragment patterns compared. Genomic heterogeneity could be detected in the DNA restriction patterns of four of these wild-type baculoviruses. Three infectious virus forms (two that are occluded in the nucleus and an extracellular virus that has budded from the plasma membrane of infected cells) of a nuclear polyhedrosis virus with multiple nucleocapsids per envelope (MNPV) from the lepidopteran insect, Autographa californica are shown to be phenotypically distinct by comparison of viral structural polypeptides by polyacrylamide gel electrophoresis and autoradiography of L[35S]methio-nine-labeled virus proteins. The three phenotypic forms were cloned by successive plaque purification and eight distinct variants were identified from 11 plaque-purified viruses by genotypec analysis with EcoR-1 and HindIII restriction endonuclease. Isolation of variants from the three phenotypic forms has shown that each of the infectious forms is heterogeneous and that no segregation of genotypes among the three forms was evident. The characteristic restriction fragment patterns of several variants were maintained upon multiple passage in cell culture.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>acmnpv</json:string><json:string>dna</json:string><json:string>viral</json:string><json:string>extracellular</json:string><json:string>endonuclease</json:string><json:string>polypeptide</json:string><json:string>baculovirus</json:string><json:string>restriction fragments</json:string><json:string>restriction patterns</json:string><json:string>agarose</json:string><json:string>extracellular virus</json:string><json:string>molecular weights</json:string><json:string>electrophoresed</json:string><json:string>restriction pattern</json:string><json:string>volkman</json:string><json:string>polyhedron</json:string><json:string>genomic</json:string><json:string>genome</json:string><json:string>polyhedrosis</json:string><json:string>viral dnas</json:string><json:string>polyacrylamide</json:string><json:string>mnpv</json:string><json:string>submolar</json:string><json:string>digestion</json:string><json:string>baculoviruses</json:string><json:string>fragment</json:string><json:string>cell culture</json:string><json:string>infectious forms</json:string><json:string>singles</json:string><json:string>restriction endonuclease</json:string><json:string>molecular weight</json:string><json:string>restriction</json:string><json:string>structural polypeptides</json:string><json:string>baculovirus dnas</json:string><json:string>molar</json:string><json:string>plaque</json:string><json:string>restriction enzymes</json:string><json:string>cell protein</json:string><json:string>genomic heterogeneity</json:string><json:string>agarose electrophoresis</json:string><json:string>virus dnas</json:string><json:string>submolar fragments</json:string><json:string>virus</json:string></teeft></keywords><author><json:item><name>Gale E. Smith</name><affiliations><json:string>Department of Entomology, Texas A & M University, College Station, Texas 77843, USA</json:string></affiliations></json:item><json:item><name>Max D. Summers</name><affiliations><json:string>Department of Entomology, Texas A & M University, College Station, Texas 77843, USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-L8912P7Z-F</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The viral DNAs from nine wild-type insect baculoviruses have been isolated and the EcoR-1 restriction endonuclease fragment patterns compared. Genomic heterogeneity could be detected in the DNA restriction patterns of four of these wild-type baculoviruses. Three infectious virus forms (two that are occluded in the nucleus and an extracellular virus that has budded from the plasma membrane of infected cells) of a nuclear polyhedrosis virus with multiple nucleocapsids per envelope (MNPV) from the lepidopteran insect, Autographa californica are shown to be phenotypically distinct by comparison of viral structural polypeptides by polyacrylamide gel electrophoresis and autoradiography of L[35S]methio-nine-labeled virus proteins. The three phenotypic forms were cloned by successive plaque purification and eight distinct variants were identified from 11 plaque-purified viruses by genotypec analysis with EcoR-1 and HindIII restriction endonuclease. Isolation of variants from the three phenotypic forms has shown that each of the infectious forms is heterogeneous and that no segregation of genotypes among the three forms was evident. The characteristic restriction fragment patterns of several variants were maintained upon multiple passage in cell culture.</abstract><qualityIndicators><score>8.527</score><pdfWordCount>4427</pdfWordCount><pdfCharCount>32277</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>504 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>175</abstractWordCount><abstractCharCount>1275</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Analysis of baculovirus genomes with restriction endonucleases</title><pmid><json:string>18627885</json:string></pmid><pii><json:string>0042-6822(78)90193-9</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Nat. Acad. Sci. 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(1976)</json:string><json:string>Summers and Anderson, 1973</json:string><json:string>Summers, 1977</json:string><json:string>Wood, 1977</json:string><json:string>Volkman et al. (1976)</json:string><json:string>Rohrmann et al., 1978</json:string><json:string>Summers and Anderson, 1972</json:string><json:string>Pettersson et al. (1973)</json:string><json:string>Summers and Smith, 1978</json:string><json:string>Helling et al. 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Genomic heterogeneity could be detected in the DNA restriction patterns of four of these wild-type baculoviruses. Three infectious virus forms (two that are occluded in the nucleus and an extracellular virus that has budded from the plasma membrane of infected cells) of a nuclear polyhedrosis virus with multiple nucleocapsids per envelope (MNPV) from the lepidopteran insect, Autographa californica are shown to be phenotypically distinct by comparison of viral structural polypeptides by polyacrylamide gel electrophoresis and autoradiography of L[35S]methio-nine-labeled virus proteins. The three phenotypic forms were cloned by successive plaque purification and eight distinct variants were identified from 11 plaque-purified viruses by genotypec analysis with EcoR-1 and HindIII restriction endonuclease. Isolation of variants from the three phenotypic forms has shown that each of the infectious forms is heterogeneous and that no segregation of genotypes among the three forms was evident. The characteristic restriction fragment patterns of several variants were maintained upon multiple passage in cell culture.</p></abstract></profileDesc><revisionDesc><change when="1978">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-L8912P7Z-F/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>78901939</aid><ce:pii>0042-6822(78)90193-9</ce:pii><ce:doi>10.1016/0042-6822(78)90193-9</ce:doi><ce:copyright type="unknown" year="1978"></ce:copyright></item-info><head><ce:title>Analysis of baculovirus genomes with restriction endonucleases</ce:title><ce:author-group><ce:author><ce:given-name>Gale E.</ce:given-name><ce:surname>Smith</ce:surname></ce:author><ce:author><ce:given-name>Max D.</ce:given-name><ce:surname>Summers</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Entomology, Texas A & M University, College Station, Texas 77843, USA</ce:textfn></ce:affiliation></ce:author-group><ce:date-accepted day="6" month="6" year="1978"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The viral DNAs from nine wild-type insect baculoviruses have been isolated and the <ce:italic>Eco</ce:italic>R-1 restriction endonuclease fragment patterns compared. Genomic heterogeneity could be detected in the DNA restriction patterns of four of these wild-type baculoviruses. Three infectious virus forms (two that are occluded in the nucleus and an extracellular virus that has budded from the plasma membrane of infected cells) of a nuclear polyhedrosis virus with <ce:italic>m</ce:italic>ultiple nucleocapsids per envelope (MNPV) from the lepidopteran insect, <ce:italic>Autographa californica</ce:italic> are shown to be phenotypically distinct by comparison of viral structural polypeptides by polyacrylamide gel electrophoresis and autoradiography of L[<ce:sup loc="pre">35</ce:sup>S]methio-nine-labeled virus proteins. The three phenotypic forms were cloned by successive plaque purification and eight distinct variants were identified from 11 plaque-purified viruses by genotypec analysis with <ce:italic>Eco</ce:italic>R-1 and <ce:italic>Hind</ce:italic>III restriction endonuclease. Isolation of variants from the three phenotypic forms has shown that each of the infectious forms is heterogeneous and that no segregation of genotypes among the three forms was evident. The characteristic restriction fragment patterns of several variants were maintained upon multiple passage in cell culture.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Analysis of baculovirus genomes with restriction endonucleases</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Analysis of baculovirus genomes with restriction endonucleases</title></titleInfo><name type="personal"><namePart type="given">Gale E.</namePart><namePart type="family">Smith</namePart><affiliation>Department of Entomology, Texas A & M University, College Station, Texas 77843, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Max D.</namePart><namePart type="family">Summers</namePart><affiliation>Department of Entomology, Texas A & M University, College Station, Texas 77843, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1978</dateIssued><copyrightDate encoding="w3cdtf">1978</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The viral DNAs from nine wild-type insect baculoviruses have been isolated and the EcoR-1 restriction endonuclease fragment patterns compared. Genomic heterogeneity could be detected in the DNA restriction patterns of four of these wild-type baculoviruses. Three infectious virus forms (two that are occluded in the nucleus and an extracellular virus that has budded from the plasma membrane of infected cells) of a nuclear polyhedrosis virus with multiple nucleocapsids per envelope (MNPV) from the lepidopteran insect, Autographa californica are shown to be phenotypically distinct by comparison of viral structural polypeptides by polyacrylamide gel electrophoresis and autoradiography of L[35S]methio-nine-labeled virus proteins. The three phenotypic forms were cloned by successive plaque purification and eight distinct variants were identified from 11 plaque-purified viruses by genotypec analysis with EcoR-1 and HindIII restriction endonuclease. Isolation of variants from the three phenotypic forms has shown that each of the infectious forms is heterogeneous and that no segregation of genotypes among the three forms was evident. The characteristic restriction fragment patterns of several variants were maintained upon multiple passage in cell culture.</abstract><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1978</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0304-2</identifier><part><date>1978</date><detail type="volume"><number>89</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>347</start><end>655</end></extent><extent unit="pages"><start>517</start><end>527</end></extent></part></relatedItem><identifier type="istex">C45FF837DC155C02E996F19DB2AA1E5D21C421D5</identifier><identifier type="ark">ark:/67375/6H6-L8912P7Z-F</identifier><identifier type="DOI">10.1016/0042-6822(78)90193-9</identifier><identifier type="PII">0042-6822(78)90193-9</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-L8912P7Z-F/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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