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Stability of the conservative mode of nucleosome assembly

Identifieur interne : 001534 ( Istex/Corpus ); précédent : 001533; suivant : 001535

Stability of the conservative mode of nucleosome assembly

Auteurs : I. M. Leffak

Source :

RBID : ISTEX:2D00CC893DB9AA9F584D4D6C1C2644D772E734D8

Abstract

The conservative assembly of nucleosome histone octamer cores has been confirmed by electrophoretic analysis of density labeled histones following equilibrium buoyant density centrifugation. After normal replication, crosslinked octamers are shown not to contain a mixture of new and old core hiatonee. Moreover, when DNA synthesis is inhibited by ara-C nucleoaome cores are still assembled exclusively from nascent histone. Similarly, after release from cycloheximide inhibition newly synthesized core histone is conservatively deposited. Thus, a conservative mechanism of histone octamer assembly occurs when nascent histone is present in the normal stoichiometry to nascent DNA and when chromatin is assembled in nascent histone or nascent DNA excess.

Url:
DOI: 10.1093/nar/11.9.2717

Links to Exploration step

ISTEX:2D00CC893DB9AA9F584D4D6C1C2644D772E734D8

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Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021