Identification, characterization and functional analysis of a chitin synthase gene in the brown citrus aphid, Toxoptera citricida (Hemiptera, Aphididae)
Identifieur interne : 001419 ( Istex/Corpus ); précédent : 001418; suivant : 001420Identification, characterization and functional analysis of a chitin synthase gene in the brown citrus aphid, Toxoptera citricida (Hemiptera, Aphididae)
Auteurs : F. Shang ; Y. Xiong ; W. Xia ; D. Wei ; D. Wei ; J. WangSource :
- Insect Molecular Biology [ 0962-1075 ] ; 2016-08.
Abstract
Chitin synthase (CHS) is a crucial enzyme involved in the final step of the insect chitin biosynthetic pathway. In this study, we cloned the full‐length cDNA sequence of a chitin synthase gene (TCiCHS) from the brown citrus aphid, Toxoptera citricida, an important citrus pest and the main vector of citrus tristeza virus worldwide. TCiCHS was expressed during the entire lifecycle and in all insect tissues examined. Expression was highest in first–second‐instar nymphs, nymph–adult transitions and in the abdomen (6.7‐fold higher than head). Embryos had a higher expression level than the integument. Fourth‐instar nymphs were exposed to 5 and 500 mg/l concentrations of the chitin synthesis inhibitor diflubenzuron (DFB) for 48 h and had the highest mortality at the 500 mg/l concentration. The mRNA expression levels of TCiCHS were significantly enhanced upon the exposure of nymphs to both low and high DFB concentrations. Silencing of TCiCHS occurred through plant‐mediated double‐stranded RNA (dsRNA) feeding. Most dsRNA‐fed nymphs were unable to moult to the next stage, and the expression of TCiCHS decreased 48% compared with controls. These results demonstrate that TCiCHS plays an important role in nymph to adult development, is possibly help identify molecular targets for To. citricida control.
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DOI: 10.1111/imb.12228
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Xia</name><affiliation><mods:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Zhaotong City Branch of Yunnan Provincial Tobacco Corporation, Zhaotong, China</mods:affiliation></affiliation></author><author><name sortKey="Wei, D" sort="Wei, D" uniqKey="Wei D" first="D." last="Wei">D. Wei</name><affiliation><mods:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</mods:affiliation></affiliation></author><author><name sortKey="Wei, D" sort="Wei, D" uniqKey="Wei D" first="D." last="Wei">D. 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Xia</name><affiliation><mods:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Zhaotong City Branch of Yunnan Provincial Tobacco Corporation, Zhaotong, China</mods:affiliation></affiliation></author><author><name sortKey="Wei, D" sort="Wei, D" uniqKey="Wei D" first="D." last="Wei">D. Wei</name><affiliation><mods:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</mods:affiliation></affiliation></author><author><name sortKey="Wei, D" sort="Wei, D" uniqKey="Wei D" first="D." last="Wei">D. 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Wang</name><affiliation><mods:affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: jjwang7008@yahoo.com</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Insect Molecular Biology</title><title level="j" type="alt">INSECT MOLECULAR BIOLOGY</title><idno type="ISSN">0962-1075</idno><idno type="eISSN">1365-2583</idno><imprint><biblScope unit="vol">25</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="422">422</biblScope><biblScope unit="page" to="430">430</biblScope><biblScope unit="page-count">9</biblScope><date type="published" when="2016-08">2016-08</date></imprint><idno type="ISSN">0962-1075</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0962-1075</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Chitin synthase (CHS) is a crucial enzyme involved in the final step of the insect chitin biosynthetic pathway. In this study, we cloned the full‐length cDNA sequence of a chitin synthase gene (TCiCHS) from the brown citrus aphid, Toxoptera citricida, an important citrus pest and the main vector of citrus tristeza virus worldwide. TCiCHS was expressed during the entire lifecycle and in all insect tissues examined. Expression was highest in first–second‐instar nymphs, nymph–adult transitions and in the abdomen (6.7‐fold higher than head). Embryos had a higher expression level than the integument. Fourth‐instar nymphs were exposed to 5 and 500 mg/l concentrations of the chitin synthesis inhibitor diflubenzuron (DFB) for 48 h and had the highest mortality at the 500 mg/l concentration. The mRNA expression levels of TCiCHS were significantly enhanced upon the exposure of nymphs to both low and high DFB concentrations. Silencing of TCiCHS occurred through plant‐mediated double‐stranded RNA (dsRNA) feeding. Most dsRNA‐fed nymphs were unable to moult to the next stage, and the expression of TCiCHS decreased 48% compared with controls. These results demonstrate that TCiCHS plays an important role in nymph to adult development, is possibly help identify molecular targets for To. citricida control.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>chitin</json:string><json:string>tcichs</json:string><json:string>citricida</json:string><json:string>synthase</json:string><json:string>aphid</json:string><json:string>biol</json:string><json:string>dsrna</json:string><json:string>nymph</json:string><json:string>biochem</json:string><json:string>chs1</json:string><json:string>chitin synthase gene</json:string><json:string>insect biochem</json:string><json:string>cdna</json:string><json:string>moulting</json:string><json:string>cuticle</json:string><json:string>merzendorfer</json:string><json:string>primer</json:string><json:string>toxoptera</json:string><json:string>chitin synthase</json:string><json:string>citrus</json:string><json:string>rnai</json:string><json:string>zhang</json:string><json:string>body part</json:string><json:string>chitin synthesis</json:string><json:string>synthases</json:string><json:string>wang</json:string><json:string>integument</json:string><json:string>qpcr</json:string><json:string>entomological</json:string><json:string>royal entomological society</json:string><json:string>bansal</json:string><json:string>apterous</json:string><json:string>alate</json:string><json:string>peritrophic</json:string><json:string>diflubenzuron</json:string><json:string>hemiptera</json:string><json:string>dstcichs</json:string><json:string>brown citrus aphid</json:string><json:string>castaneum</json:string><json:string>lugens</json:string><json:string>arakane</json:string><json:string>toxoptera citricida</json:string><json:string>shang</json:string><json:string>glycine</json:string><json:string>significant difference</json:string><json:string>phylogenetic</json:string><json:string>embryo</json:string><json:string>cumulative mortality</json:string><json:string>splice variant</json:string><json:string>expression profile</json:string><json:string>biological replicates</json:string><json:string>moulting rate</json:string><json:string>peritrophic membrane</json:string><json:string>relative expression</json:string><json:string>different developmental stage</json:string><json:string>expression level</json:string><json:string>alate adult</json:string><json:string>apterous adult</json:string><json:string>phylogenetic analysis</json:string><json:string>functional analysis</json:string><json:string>chitin biosynthesis</json:string><json:string>nilaparvata lugens</json:string><json:string>chitin synthases</json:string><json:string>splice</json:string><json:string>arbitrary value</json:string><json:string>drosophila melanogaster</json:string><json:string>tribolium castaneum</json:string><json:string>adult transition</json:string><json:string>dsrna synthesis</json:string><json:string>plutella xylostella</json:string><json:string>tcichs expression</json:string><json:string>amino acid sequence</json:string><json:string>laodelphax striatellus</json:string><json:string>aphis glycine</json:string><json:string>biological function</json:string><json:string>specific primer</json:string><json:string>transmembrane helix</json:string><json:string>locusta migratoria manilensis</json:string><json:string>insect growth regulator</json:string></teeft></keywords><author><json:item><name>F. 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In this study, we cloned the full‐length cDNA sequence of a chitin synthase gene (TCiCHS) from the brown citrus aphid, Toxoptera citricida, an important citrus pest and the main vector of citrus tristeza virus worldwide. TCiCHS was expressed during the entire lifecycle and in all insect tissues examined. Expression was highest in first–second‐instar nymphs, nymph–adult transitions and in the abdomen (6.7‐fold higher than head). Embryos had a higher expression level than the integument. Fourth‐instar nymphs were exposed to 5 and 500 mg/l concentrations of the chitin synthesis inhibitor diflubenzuron (DFB) for 48 h and had the highest mortality at the 500 mg/l concentration. The mRNA expression levels of TCiCHS were significantly enhanced upon the exposure of nymphs to both low and high DFB concentrations. Silencing of TCiCHS occurred through plant‐mediated double‐stranded RNA (dsRNA) feeding. Most dsRNA‐fed nymphs were unable to moult to the next stage, and the expression of TCiCHS decreased 48% compared with controls. 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<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract style="main"><head>Abstract</head><p>Chitin synthase (CHS) is a crucial enzyme involved in the final step of the insect chitin biosynthetic pathway. In this study, we cloned the full‐length cDNA sequence of a chitin synthase gene (<hi rend="italic">TCiCHS</hi>) from the brown citrus aphid, <hi rend="italic">Toxoptera citricida</hi>, an important citrus pest and the main vector of citrus tristeza virus worldwide. <hi rend="italic">TCiCHS</hi> was expressed during the entire lifecycle and in all insect tissues examined. Expression was highest in first–second‐instar nymphs, nymph–adult transitions and in the abdomen (6.7‐fold higher than head). Embryos had a higher expression level than the integument. Fourth‐instar nymphs were exposed to 5 and 500 mg/l concentrations of the chitin synthesis inhibitor diflubenzuron (DFB) for 48 h and had the highest mortality at the 500 mg/l concentration. The mRNA expression levels of <hi rend="italic">TCiCHS</hi> were significantly enhanced upon the exposure of nymphs to both low and high DFB concentrations. Silencing of <hi rend="italic">TCiCHS</hi> occurred through plant‐mediated double‐stranded RNA (dsRNA) feeding. Most dsRNA‐fed nymphs were unable to moult to the next stage, and the expression of <hi rend="italic">TCiCHS</hi> decreased 48% compared with controls. These results demonstrate that <hi rend="italic">TCiCHS</hi> plays an important role in nymph to adult development, is possibly help identify molecular targets for <hi rend="italic">To. citricida</hi> control.</p></abstract><textClass><keywords><term xml:id="imb12228-kwd-0001"><hi rend="italic">Toxoptera citricida</hi></term><term xml:id="imb12228-kwd-0002">chitin synthase</term><term xml:id="imb12228-kwd-0003">diflubenzuron</term><term xml:id="imb12228-kwd-0004">RNA interference</term></keywords><keywords rend="articleCategory"><term>Original Article</term></keywords><keywords rend="tocHeading1"><term>Original Articles</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-Q9D17XVZ-H/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en" xml:id="imb12228"><header><publicationMeta level="product"><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2583</doi><issn type="print">0962-1075</issn><issn type="electronic">1365-2583</issn><idGroup><id type="product" value="IMB"></id></idGroup><titleGroup><title type="main" sort="INSECT MOLECULAR BIOLOGY">Insect Molecular Biology</title><title type="short">Insect Mol Biol</title></titleGroup></publicationMeta><publicationMeta level="part" position="40"><doi>10.1111/imb.2016.25.issue-4</doi><copyright ownership="thirdParty">© 2016 The Royal Entomological Society</copyright><numberingGroup><numbering type="journalVolume" number="25">25</numbering><numbering type="journalIssue">4</numbering></numberingGroup><coverDate startDate="2016-08">August 2016</coverDate></publicationMeta><publicationMeta level="unit" position="80" type="article" status="forIssue"><doi>10.1111/imb.12228</doi><idGroup><id type="unit" value="IMB12228"></id></idGroup><countGroup><count type="pageTotal" number="9"></count></countGroup><titleGroup><title type="articleCategory">Original Article</title><title type="tocHeading1">Original Articles</title></titleGroup><copyright ownership="thirdParty">© 2016 The Royal Entomological Society</copyright><eventGroup><event type="xmlCreated" agent="Cenveo Publisher Services" date="2016-02-28"></event><event type="firstOnline" date="2016-03-17"></event><event type="publishedOnlineEarlyUnpaginated" date="2016-03-17"></event><event type="publishedOnlineFinalForm" date="2016-07-05"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:5.0.6 mode:FullText" date="2017-02-10"></event></eventGroup><numberingGroup><numbering type="pageFirst">422</numbering><numbering type="pageLast">430</numbering></numberingGroup><correspondenceTo>Correspondence: Dr Jin‐Jun Wang, College of Plant Protection, Southwest University, Chongqing 400715, China. Tel.: + 86 23 68250255; fax: + 86 23 68251269; e‐mail: <email>jjwang7008@yahoo.com</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:IMB.IMB12228.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Identification, characterization and functional analysis of a chitin synthase gene in the brown citrus aphid, <i>Toxoptera citricida</i> (Hemiptera, Aphididae)</title><title type="short">Chitin synthase gene in brown citrus aphid</title><title type="shortAuthors">F. Shang <i>et al.</i></title></titleGroup><creators><creator affiliationRef="#imb12228-aff-0001" creatorRole="author" xml:id="imb12228-cr-0001"><personName><givenNames>F.</givenNames><familyName>Shang</familyName></personName></creator><creator affiliationRef="#imb12228-aff-0001" creatorRole="author" xml:id="imb12228-cr-0002"><personName><givenNames>Y.</givenNames><familyName>Xiong</familyName></personName></creator><creator affiliationRef="#imb12228-aff-0001 #imb12228-aff-0002" creatorRole="author" xml:id="imb12228-cr-0003"><personName><givenNames>W.‐K.</givenNames><familyName>Xia</familyName></personName></creator><creator affiliationRef="#imb12228-aff-0001" creatorRole="author" xml:id="imb12228-cr-0004"><personName><givenNames>D.‐D.</givenNames><familyName>Wei</familyName></personName></creator><creator affiliationRef="#imb12228-aff-0001" creatorRole="author" xml:id="imb12228-cr-0005"><personName><givenNames>D.</givenNames><familyName>Wei</familyName></personName></creator><creator affiliationRef="#imb12228-aff-0001" corresponding="yes" creatorRole="author" xml:id="imb12228-cr-0006"><personName><givenNames>J.‐J.</givenNames><familyName>Wang</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="CN" type="organization" xml:id="imb12228-aff-0001"><orgDiv>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection</orgDiv><orgName>Southwest University</orgName><address><city>Chongqing</city><country>China</country></address></affiliation><affiliation countryCode="CN" type="organization" xml:id="imb12228-aff-0002"><orgName>Zhaotong City Branch of Yunnan Provincial Tobacco Corporation</orgName><address><city>Zhaotong</city><country>China</country></address></affiliation></affiliationGroup><keywordGroup type="author"><keyword xml:id="imb12228-kwd-0001"><i>Toxoptera citricida</i></keyword><keyword xml:id="imb12228-kwd-0002">chitin synthase</keyword><keyword xml:id="imb12228-kwd-0003">diflubenzuron</keyword><keyword xml:id="imb12228-kwd-0004">RNA interference</keyword></keywordGroup><fundingInfo><fundingAgency>Agro‐scientific Research</fundingAgency><fundingNumber>201203038</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Modern Agro‐industry (Citrus) Technology Research System of China</fundingAgency><fundingNumber>CARS‐27</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Central Universities of China</fundingAgency><fundingNumber>XDJK2016D039</fundingNumber></fundingInfo><supportingInformation><p>Additional Supporting Information may be found in the online version of this article at the publisher's web‐site:</p><supportingInfoItem><mediaResource alt="application" href="urn-x:wiley:09621075:media:imb12228:imb12228-sup-0001-suppinfo01"></mediaResource><caption><p><b>Figure S1.</b> Nucleotide and deduced amino acid sequences of <i>Toxoptera citricida chitin synthae</i> (<i>TCiCHS</i>). The start codon (ATG) and stop codon (TGA) are highlighted in black; the 16 hydrophobic, membrane‐spanning α‐helices are highlighted in grey. The 12 putative N‐glycosylation sites are underlined; the two motifs (EDR and TWGTR) of the highly conserved central domain are boxed.</p></caption></supportingInfoItem><supportingInfoItem><mediaResource alt="application" href="urn-x:wiley:09621075:media:imb12228:imb12228-sup-0002-suppinfo02"></mediaResource><caption><p><b>Table S1.</b> Sequences and relevant information used for phylogenetic analysis of the chitin synthase gene.</p></caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main"><title type="main">Abstract</title><p>Chitin synthase (CHS) is a crucial enzyme involved in the final step of the insect chitin biosynthetic pathway. In this study, we cloned the full‐length cDNA sequence of a chitin synthase gene (<i>TCiCHS</i>) from the brown citrus aphid, <i>Toxoptera citricida</i>, an important citrus pest and the main vector of citrus tristeza virus worldwide. <i>TCiCHS</i> was expressed during the entire lifecycle and in all insect tissues examined. Expression was highest in first–second‐instar nymphs, nymph–adult transitions and in the abdomen (6.7‐fold higher than head). Embryos had a higher expression level than the integument. Fourth‐instar nymphs were exposed to 5 and 500 mg/l concentrations of the chitin synthesis inhibitor diflubenzuron (DFB) for 48 h and had the highest mortality at the 500 mg/l concentration. The mRNA expression levels of <i>TCiCHS</i> were significantly enhanced upon the exposure of nymphs to both low and high DFB concentrations. Silencing of <i>TCiCHS</i> occurred through plant‐mediated double‐stranded RNA (dsRNA) feeding. Most dsRNA‐fed nymphs were unable to moult to the next stage, and the expression of <i>TCiCHS</i> decreased 48% compared with controls. These results demonstrate that <i>TCiCHS</i> plays an important role in nymph to adult development, is possibly help identify molecular targets for <i>To. citricida</i> control.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Identification, characterization and functional analysis of a chitin synthase gene in the brown citrus aphid, Toxoptera citricida (Hemiptera, Aphididae)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Chitin synthase gene in brown citrus aphid</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Identification, characterization and functional analysis of a chitin synthase gene in the brown citrus aphid, Toxoptera citricida (Hemiptera, Aphididae)</title></titleInfo><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Shang</namePart><affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Xiong</namePart><affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W.‐K.</namePart><namePart type="family">Xia</namePart><affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</affiliation><affiliation>Zhaotong City Branch of Yunnan Provincial Tobacco Corporation, Zhaotong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.‐D.</namePart><namePart type="family">Wei</namePart><affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Wei</namePart><affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.‐J.</namePart><namePart type="family">Wang</namePart><affiliation>Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China</affiliation><affiliation>E-mail: jjwang7008@yahoo.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2016-08</dateIssued><dateCreated encoding="w3cdtf">2016-02-28</dateCreated><copyrightDate encoding="w3cdtf">2016</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract>Chitin synthase (CHS) is a crucial enzyme involved in the final step of the insect chitin biosynthetic pathway. In this study, we cloned the full‐length cDNA sequence of a chitin synthase gene (TCiCHS) from the brown citrus aphid, Toxoptera citricida, an important citrus pest and the main vector of citrus tristeza virus worldwide. TCiCHS was expressed during the entire lifecycle and in all insect tissues examined. Expression was highest in first–second‐instar nymphs, nymph–adult transitions and in the abdomen (6.7‐fold higher than head). Embryos had a higher expression level than the integument. Fourth‐instar nymphs were exposed to 5 and 500 mg/l concentrations of the chitin synthesis inhibitor diflubenzuron (DFB) for 48 h and had the highest mortality at the 500 mg/l concentration. The mRNA expression levels of TCiCHS were significantly enhanced upon the exposure of nymphs to both low and high DFB concentrations. Silencing of TCiCHS occurred through plant‐mediated double‐stranded RNA (dsRNA) feeding. Most dsRNA‐fed nymphs were unable to moult to the next stage, and the expression of TCiCHS decreased 48% compared with controls. These results demonstrate that TCiCHS plays an important role in nymph to adult development, is possibly help identify molecular targets for To. citricida control.</abstract><note type="funding">Agro‐scientific Research - No. 201203038; </note><note type="funding">Modern Agro‐industry (Citrus) Technology Research System of China - No. CARS‐27; </note><note type="funding">Central Universities of China - No. XDJK2016D039; </note><subject><genre>keywords</genre><topic>Toxoptera citricida</topic><topic>chitin synthase</topic><topic>diflubenzuron</topic><topic>RNA interference</topic></subject><relatedItem type="host"><titleInfo><title>Insect Molecular Biology</title></titleInfo><titleInfo type="abbreviated"><title>Insect Mol Biol</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><note type="content"> Additional Supporting Information may be found in the online version of this article at the publisher's web‐site:Supporting Info Item: Figure S1. Nucleotide and deduced amino acid sequences of Toxoptera citricida chitin synthae (TCiCHS). The start codon (ATG) and stop codon (TGA) are highlighted in black; the 16 hydrophobic, membrane‐spanning α‐helices are highlighted in grey. The 12 putative N‐glycosylation sites are underlined; the two motifs (EDR and TWGTR) of the highly conserved central domain are boxed. - Table S1. Sequences and relevant information used for phylogenetic analysis of the chitin synthase gene. - </note><subject><genre>article-category</genre><topic>Original Article</topic><topic>Original Articles</topic></subject><identifier type="ISSN">0962-1075</identifier><identifier type="eISSN">1365-2583</identifier><identifier type="DOI">10.1111/(ISSN)1365-2583</identifier><identifier type="PublisherID">IMB</identifier><part><date>2016</date><detail type="volume"><caption>vol.</caption><number>25</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>422</start><end>430</end><total>9</total></extent></part></relatedItem><relatedItem type="references" displayLabel="imb12228-cit-0001"><titleInfo><title>Significance of the sulfonylurea receptor (SUR) as the target of diflubenzuron in chitin synthesis inhibition in Drosophila melanogaster and Blattella germanica</title></titleInfo><name type="personal"><namePart type="given">G.E.</namePart><namePart type="family">Abo‐Elghar</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Fujiyoshi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Matsumura</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Abo‐Elghar, G.E., Fujiyoshi, P. and Matsumura, F. 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Mol Biol Rep 41: 4177–4186.</note><part><date>2014</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><extent unit="pages"><start>4177</start><end>4186</end></extent></part><relatedItem type="host"><titleInfo><title>Mol Biol Rep</title></titleInfo><part><date>2014</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><extent unit="pages"><start>4177</start><end>4186</end></extent></part></relatedItem></relatedItem><identifier type="istex">39B3571A73E359FBDFC46A607183F760AA92FF5E</identifier><identifier type="ark">ark:/67375/WNG-Q9D17XVZ-H</identifier><identifier type="DOI">10.1111/imb.12228</identifier><identifier type="ArticleID">IMB12228</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2016 The Royal Entomological Society© 2016 The Royal Entomological Society</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-15</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-Q9D17XVZ-H/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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