Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection
Identifieur interne : 001328 ( Istex/Corpus ); précédent : 001327; suivant : 001329Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection
Auteurs : Pascal Bouffard ; Jean-Pierre Lamelin ; Fabien Zoulim ; Daniel Lepot ; Christian TrepoSource :
- Journal of Medical Virology [ 0146-6615 ] ; 1992-08.
English descriptors
- Teeft :
- Assay, Blot assay, Bouffard, Cell proliferation, Chronic hepatitis, Cona, Hbsag, Hepatitis, Human leukocytes, Human pbmc, Hybridization, Korba, Level increase, Lymphocyte, Mitogen, Mitogen exposure, Nylon membrane, Pbmc, Pbmc culture, Pbmc proliferation, Peripheral blood, Replicative, Replicative forms, Sodium acetate, Southern blot analysis, Southern blot assay, Trepo, Unstimulated, Unstimulated cultures, Viral, Viral genome, Virology, Virus infection, Woodchuck.
Abstract
Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response. By contrast, both bacterial lipopolysaccharide (LPS) and interleukin 2 (IL2) failed to do so. Dot blot assays were used to monitor HBV DNA level increase within PBMC. Following mitogen exposure HBV DNA levels increased above pre‐stimulation levels in 19/25 PHA cultures, 6/15 ConA cultures, 1/15 LPS cultures, and 1/15 IL2 cultures. In 15 patients, Southern blot analysis was carried out before and after PHA exposure. In 13/15 cases, a single 3.2 Kb band was observed in un‐stimulated cultures as well as in PHA cultures even though PHA induced a HBV DNA increase. One case exhibited bands migrating faster than the 3.2 Kb signal, compatible with replicating intermediates and one case provided evidence of viral concatemers within PBMC after PHA stimulation. No HBV DNA was detected in the culture supernatants. The increase of HBV DNA level in PBMC induced by mitogen was strongly associated with an increase in HBV DNA expression (HBV RNA and HBs antigen). These studies indicate that HBV DNA present in human PBMC does represent a potential reservoir for infection with endogenous reactivation following PBMC activation. © 1992 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.1890370404
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ISTEX:D35D1D0F4B79F61611224364E4B919A615C55CACLe document en format XML
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France</mods:affiliation></affiliation></author><author><name sortKey="Lepot, Daniel" sort="Lepot, Daniel" uniqKey="Lepot D" first="Daniel" last="Lepot">Daniel Lepot</name><affiliation><mods:affiliation>INSERM U271, Lyon, France</mods:affiliation></affiliation></author><author><name sortKey="Trepo, Christian" sort="Trepo, Christian" uniqKey="Trepo C" first="Christian" last="Trepo">Christian Trepo</name><affiliation><mods:affiliation>INSERM U271, Lyon, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: INSERM U271,151 Cours Albert Thomas, 69424 Lyon cedex 03, France===</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">37</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="255">255</biblScope><biblScope unit="page" to="262">262</biblScope><biblScope unit="page-count">8</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="1992-08">1992-08</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Blot assay</term><term>Bouffard</term><term>Cell proliferation</term><term>Chronic hepatitis</term><term>Cona</term><term>Hbsag</term><term>Hepatitis</term><term>Human leukocytes</term><term>Human pbmc</term><term>Hybridization</term><term>Korba</term><term>Level increase</term><term>Lymphocyte</term><term>Mitogen</term><term>Mitogen exposure</term><term>Nylon membrane</term><term>Pbmc</term><term>Pbmc culture</term><term>Pbmc proliferation</term><term>Peripheral blood</term><term>Replicative</term><term>Replicative forms</term><term>Sodium acetate</term><term>Southern blot analysis</term><term>Southern blot assay</term><term>Trepo</term><term>Unstimulated</term><term>Unstimulated cultures</term><term>Viral</term><term>Viral genome</term><term>Virology</term><term>Virus infection</term><term>Woodchuck</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response. By contrast, both bacterial lipopolysaccharide (LPS) and interleukin 2 (IL2) failed to do so. Dot blot assays were used to monitor HBV DNA level increase within PBMC. Following mitogen exposure HBV DNA levels increased above pre‐stimulation levels in 19/25 PHA cultures, 6/15 ConA cultures, 1/15 LPS cultures, and 1/15 IL2 cultures. In 15 patients, Southern blot analysis was carried out before and after PHA exposure. In 13/15 cases, a single 3.2 Kb band was observed in un‐stimulated cultures as well as in PHA cultures even though PHA induced a HBV DNA increase. One case exhibited bands migrating faster than the 3.2 Kb signal, compatible with replicating intermediates and one case provided evidence of viral concatemers within PBMC after PHA stimulation. No HBV DNA was detected in the culture supernatants. The increase of HBV DNA level in PBMC induced by mitogen was strongly associated with an increase in HBV DNA expression (HBV RNA and HBs antigen). These studies indicate that HBV DNA present in human PBMC does represent a potential reservoir for infection with endogenous reactivation following PBMC activation. © 1992 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>pbmc</json:string><json:string>cona</json:string><json:string>mitogen</json:string><json:string>bouffard</json:string><json:string>viral</json:string><json:string>peripheral blood</json:string><json:string>assay</json:string><json:string>virology</json:string><json:string>hbsag</json:string><json:string>trepo</json:string><json:string>lymphocyte</json:string><json:string>chronic hepatitis</json:string><json:string>mitogen exposure</json:string><json:string>woodchuck</json:string><json:string>unstimulated</json:string><json:string>replicative</json:string><json:string>hybridization</json:string><json:string>korba</json:string><json:string>pbmc proliferation</json:string><json:string>southern blot assay</json:string><json:string>hepatitis</json:string><json:string>blot assay</json:string><json:string>level increase</json:string><json:string>southern blot analysis</json:string><json:string>pbmc culture</json:string><json:string>human pbmc</json:string><json:string>cell proliferation</json:string><json:string>nylon membrane</json:string><json:string>viral genome</json:string><json:string>human leukocytes</json:string><json:string>virus infection</json:string><json:string>unstimulated cultures</json:string><json:string>replicative forms</json:string><json:string>sodium acetate</json:string></teeft></keywords><author><json:item><name>Pascal Bouffard</name><affiliations><json:string>INSERM U271, Lyon, France</json:string></affiliations></json:item><json:item><name>Jean‐Pierre Lamelin</name><affiliations><json:string>INSERM U271, Lyon, France</json:string></affiliations></json:item><json:item><name>Fabien Zoulim</name><affiliations><json:string>INSERM U271, Lyon, France</json:string></affiliations></json:item><json:item><name>Daniel Lepot</name><affiliations><json:string>INSERM U271, Lyon, France</json:string></affiliations></json:item><json:item><name>Christian Trepo</name><affiliations><json:string>INSERM U271, Lyon, France</json:string><json:string>Correspondence address: INSERM U271,151 Cours Albert Thomas, 69424 Lyon cedex 03, France===</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>leukocytes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HBV DNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LPS</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IL2</value></json:item></subject><articleId><json:string>JMV1890370404</json:string></articleId><arkIstex>ark:/67375/WNG-5DJDTQNG-8</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response. By contrast, both bacterial lipopolysaccharide (LPS) and interleukin 2 (IL2) failed to do so. Dot blot assays were used to monitor HBV DNA level increase within PBMC. Following mitogen exposure HBV DNA levels increased above pre‐stimulation levels in 19/25 PHA cultures, 6/15 ConA cultures, 1/15 LPS cultures, and 1/15 IL2 cultures. In 15 patients, Southern blot analysis was carried out before and after PHA exposure. In 13/15 cases, a single 3.2 Kb band was observed in un‐stimulated cultures as well as in PHA cultures even though PHA induced a HBV DNA increase. One case exhibited bands migrating faster than the 3.2 Kb signal, compatible with replicating intermediates and one case provided evidence of viral concatemers within PBMC after PHA stimulation. No HBV DNA was detected in the culture supernatants. The increase of HBV DNA level in PBMC induced by mitogen was strongly associated with an increase in HBV DNA expression (HBV RNA and HBs antigen). These studies indicate that HBV DNA present in human PBMC does represent a potential reservoir for infection with endogenous reactivation following PBMC activation. © 1992 Wiley‐Liss, Inc.</abstract><qualityIndicators><score>9.495</score><pdfWordCount>4591</pdfWordCount><pdfCharCount>26182</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>594 x 792 pts</pdfPageSize><pdfWordsPerPage>574</pdfWordsPerPage><pdfText>true</pdfText><refBibsNative>true</refBibsNative><abstractWordCount>242</abstractWordCount><abstractCharCount>1520</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection</title><pmid><json:string>1402824</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Journal of Medical Virology</title><language><json:string>unknown</json:string></language><doi><json:string>10.1002/(ISSN)1096-9071</json:string></doi><issn><json:string>0146-6615</json:string></issn><eissn><json:string>1096-9071</json:string></eissn><publisherId><json:string>JMV</json:string></publisherId><volume>37</volume><issue>4</issue><pages><first>255</first><last>262</last><total>8</total></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Article</value></json:item><json:item><value>Articles</value></json:item></subject></host><namedEntities><unitex><date><json:string>1992</json:string></date><geogName></geogName><orgName><json:string>Wiley-Liss, Inc</json:string><json:string>LISS, INC.</json:string><json:string>Abbott Laboratories</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Jean-Pierre Lamelin</json:string><json:string>Albert Thomas</json:string><json:string>I. Correlation</json:string><json:string>Christian Trepo</json:string><json:string>Galibert</json:string><json:string>Daniel Lepot</json:string><json:string>Fabien Zoulim</json:string><json:string>C. Trepo</json:string><json:string>P.H. Hayashi</json:string><json:string>P. Bouffard</json:string><json:string>Dry L. Cova</json:string></persName><placeName><json:string>France</json:string><json:string>Lyon</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Lobbiani et al., 1990</json:string><json:string>Lamelin and Trepo, 19901</json:string><json:string>Peavy et al., 19701</json:string><json:string>Sugai and Okamoto, 19891</json:string><json:string>Injo et al., 1983</json:string><json:string>Pontisso et al., 19911</json:string><json:string>Zoulim et al., 19901</json:string><json:string>Neurath et al., 1990</json:string><json:string>Korba et al.</json:string><json:string>Pezzella et al., 19891</json:string><json:string>Bouffard et al., 1990</json:string><json:string>Anastassakos et al., 1987</json:string><json:string>Hadchouel et al., 1988</json:string><json:string>Elfassi et al., 19841</json:string><json:string>Feray et al., 19901</json:string><json:string>Bouffard et al.</json:string><json:string>Baginski et al., 19911</json:string><json:string>Colucci et al., 19881</json:string><json:string>Parvaz et al., 1987</json:string><json:string>Korba et al., 1988</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-5DJDTQNG-8</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Infectious Diseases</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences 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Leukocytes</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><availability><licence>Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company</licence></availability><date type="published" when="1992-08"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection</title><title level="a" type="short" xml:lang="en">Mitogens Activate HBV in Human Leukocytes</title><author xml:id="author-0000"><persName><forename type="first">Pascal</forename><surname>Bouffard</surname></persName><affiliation><address><addrLine>INSERM U271</addrLine><addrLine>Lyon, France</addrLine><country key="FR" xml:lang="en">FRANCE</country></address></affiliation></author><author xml:id="author-0001"><persName><forename type="first">Jean‐Pierre</forename><surname>Lamelin</surname></persName><affiliation><address><addrLine>INSERM U271</addrLine><addrLine>Lyon, France</addrLine><country key="FR" xml:lang="en">FRANCE</country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">Fabien</forename><surname>Zoulim</surname></persName><affiliation><address><addrLine>INSERM U271</addrLine><addrLine>Lyon, France</addrLine><country key="FR" xml:lang="en">FRANCE</country></address></affiliation></author><author xml:id="author-0003"><persName><forename 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<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract xml:lang="en" style="main"><head>Abstract</head><p>Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response. By contrast, both bacterial lipopolysaccharide (LPS) and interleukin 2 (IL2) failed to do so. Dot blot assays were used to monitor HBV DNA level increase within PBMC. Following mitogen exposure HBV DNA levels increased above pre‐stimulation levels in 19/25 PHA cultures, 6/15 ConA cultures, 1/15 LPS cultures, and 1/15 IL2 cultures.</p><p>In 15 patients, Southern blot analysis was carried out before and after PHA exposure. In 13/15 cases, a single 3.2 Kb band was observed in un‐stimulated cultures as well as in PHA cultures even though PHA induced a HBV DNA increase. One case exhibited bands migrating faster than the 3.2 Kb signal, compatible with replicating intermediates and one case provided evidence of viral concatemers within PBMC after PHA stimulation. No HBV DNA was detected in the culture supernatants. The increase of HBV DNA level in PBMC induced by mitogen was strongly associated with an increase in HBV DNA expression (HBV RNA and HBs antigen). These studies indicate that HBV DNA present in human PBMC does represent a potential reservoir for infection with endogenous reactivation following PBMC activation. © 1992 Wiley‐Liss, Inc.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="kwd1">leukocytes</term><term xml:id="kwd2">HBV DNA</term><term xml:id="kwd3">LPS</term><term xml:id="kwd4">IL2</term></keywords><keywords rend="articleCategory"><term>Article</term></keywords><keywords rend="tocHeading1"><term>Articles</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-5DJDTQNG-8/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>New York</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1096-9071</doi><issn type="print">0146-6615</issn><issn type="electronic">1096-9071</issn><idGroup><id type="product" value="JMV"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF MEDICAL VIROLOGY">Journal of Medical Virology</title><title type="short">J. Med. Virol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="40"><doi origin="wiley" registered="yes">10.1002/jmv.v37:4</doi><numberingGroup><numbering type="journalVolume" number="37">37</numbering><numbering type="journalIssue">4</numbering></numberingGroup><coverDate startDate="1992-08">August 1992</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="4" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jmv.1890370404</doi><idGroup><id type="unit" value="JMV1890370404"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><titleGroup><title type="articleCategory">Article</title><title type="tocHeading1">Articles</title></titleGroup><copyright ownership="publisher">Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company</copyright><eventGroup><event type="manuscriptAccepted" date="1992-01-20"></event><event type="firstOnline" date="2005-12-07"></event><event type="publishedOnlineFinalForm" date="2005-12-07"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-15"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst">255</numbering><numbering type="pageLast">262</numbering></numberingGroup><correspondenceTo>INSERM U271,151 Cours Albert Thomas, 69424 Lyon cedex 03, France===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JMV.JMV1890370404.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="6"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="32"></count></countGroup><titleGroup><title type="main" xml:lang="en">Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection</title><title type="short" xml:lang="en">Mitogens Activate HBV in Human Leukocytes</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Pascal</givenNames><familyName>Bouffard</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Jean‐Pierre</givenNames><familyName>Lamelin</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Fabien</givenNames><familyName>Zoulim</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Daniel</givenNames><familyName>Lepot</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Christian</givenNames><familyName>Trepo</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="FR" type="organization"><unparsedAffiliation>INSERM U271, Lyon, France</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">leukocytes</keyword><keyword xml:id="kwd2">HBV DNA</keyword><keyword xml:id="kwd3">LPS</keyword><keyword xml:id="kwd4">IL2</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response. By contrast, both bacterial lipopolysaccharide (LPS) and interleukin 2 (IL2) failed to do so. Dot blot assays were used to monitor HBV DNA level increase within PBMC. Following mitogen exposure HBV DNA levels increased above pre‐stimulation levels in 19/25 PHA cultures, 6/15 ConA cultures, 1/15 LPS cultures, and 1/15 IL2 cultures.</p><p>In 15 patients, Southern blot analysis was carried out before and after PHA exposure. In 13/15 cases, a single 3.2 Kb band was observed in un‐stimulated cultures as well as in PHA cultures even though PHA induced a HBV DNA increase. One case exhibited bands migrating faster than the 3.2 Kb signal, compatible with replicating intermediates and one case provided evidence of viral concatemers within PBMC after PHA stimulation. No HBV DNA was detected in the culture supernatants. The increase of HBV DNA level in PBMC induced by mitogen was strongly associated with an increase in HBV DNA expression (HBV RNA and HBs antigen). These studies indicate that HBV DNA present in human PBMC does represent a potential reservoir for infection with endogenous reactivation following PBMC activation. © 1992 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Mitogens Activate HBV in Human Leukocytes</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection</title></titleInfo><name type="personal"><namePart type="given">Pascal</namePart><namePart type="family">Bouffard</namePart><affiliation>INSERM U271, Lyon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean‐Pierre</namePart><namePart type="family">Lamelin</namePart><affiliation>INSERM U271, Lyon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Fabien</namePart><namePart type="family">Zoulim</namePart><affiliation>INSERM U271, Lyon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Daniel</namePart><namePart type="family">Lepot</namePart><affiliation>INSERM U271, Lyon, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christian</namePart><namePart type="family">Trepo</namePart><affiliation>INSERM U271, Lyon, France</affiliation><affiliation>Correspondence address: INSERM U271,151 Cours Albert Thomas, 69424 Lyon cedex 03, France===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">1992-08</dateIssued><dateValid encoding="w3cdtf">1992-01-20</dateValid><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">6</extent><extent unit="tables">1</extent><extent unit="references">32</extent></physicalDescription><abstract lang="en">Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response. By contrast, both bacterial lipopolysaccharide (LPS) and interleukin 2 (IL2) failed to do so. Dot blot assays were used to monitor HBV DNA level increase within PBMC. Following mitogen exposure HBV DNA levels increased above pre‐stimulation levels in 19/25 PHA cultures, 6/15 ConA cultures, 1/15 LPS cultures, and 1/15 IL2 cultures. In 15 patients, Southern blot analysis was carried out before and after PHA exposure. In 13/15 cases, a single 3.2 Kb band was observed in un‐stimulated cultures as well as in PHA cultures even though PHA induced a HBV DNA increase. One case exhibited bands migrating faster than the 3.2 Kb signal, compatible with replicating intermediates and one case provided evidence of viral concatemers within PBMC after PHA stimulation. No HBV DNA was detected in the culture supernatants. The increase of HBV DNA level in PBMC induced by mitogen was strongly associated with an increase in HBV DNA expression (HBV RNA and HBs antigen). These studies indicate that HBV DNA present in human PBMC does represent a potential reservoir for infection with endogenous reactivation following PBMC activation. © 1992 Wiley‐Liss, Inc.</abstract><subject lang="en"><genre>keywords</genre><topic>leukocytes</topic><topic>HBV DNA</topic><topic>LPS</topic><topic>IL2</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Medical Virology</title></titleInfo><titleInfo type="abbreviated"><title>J. Med. 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Journal of Hepatology 12: 150–156.</note><part><date>1990</date><detail type="volume"><caption>vol.</caption><number>12</number></detail><extent unit="pages"><start>150</start><end>156</end></extent></part><relatedItem type="host"><titleInfo><title>Journal of Hepatology</title></titleInfo><part><date>1990</date><detail type="volume"><caption>vol.</caption><number>12</number></detail><extent unit="pages"><start>150</start><end>156</end></extent></part></relatedItem></relatedItem><identifier type="istex">D35D1D0F4B79F61611224364E4B919A615C55CAC</identifier><identifier type="ark">ark:/67375/WNG-5DJDTQNG-8</identifier><identifier type="DOI">10.1002/jmv.1890370404</identifier><identifier type="ArticleID">JMV1890370404</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-15</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-5DJDTQNG-8/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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