Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces
Identifieur interne : 001323 ( Istex/Corpus ); précédent : 001322; suivant : 001324Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces
Auteurs : Asya Levina ; Inna Pyshnaya ; Marina Repkova ; Vera Rar ; Valentina ZarytovaSource :
- Biotechnology Journal [ 1860-6768 ] ; 2007-07.
English descriptors
- Teeft :
- Babesia, Biotechnol, Biotin label, Chips, Chromogenic substrates, Derivative, Epoxy glass slides, Fragment, Glass slides, Gmbh, Hybridization, Immobilization, Kgaa, Lavsan, Ligation, Ligation product, Nucleic, Nucleic acids, Nucleosides nucleotides, Nucleotide, Nylon, Nylon membranes, Oligonucleotide, Oligonucleotides, Positive control, Potassium phosphate buffer, Room temperature, Rrna gene, Seite, Short oligonucleotides, Solid support, Solid surfaces, Surface capacity, Tandem, Unmodified, Unmodified glass slides, Verlag, Verlag gmbh, Weinheim, Weinheim seite.
Abstract
Various materials, such as glass, plastic, metals, etc., are utilized for preparing DNA chips. In each particular case special approaches are used for immobilization of different oligonucleotide derivatives on the solid supports. We describe a general technique for DNA chips preparation on various unmodified surfaces using one type of oligonucleotide derivative, polylysine‐oligonucleotide conjugates (PL‐oligo). A long polyamine spacer in the PL‐oligo conjugates provides a durable irreversible non‐covalent immobilization onto a variety of solid supports and enough distance between oligonucleotides and the surface. The resulting DNA chips were shown to be useful for the detection of PCR DNA fragments and to be sensitive to single nucleotide discrepancies. They represent a promising instrument for revealing genetic diseases, genotyping viruses and bacteria, and for displaying their drug‐resistant strains.
Url:
DOI: 10.1002/biot.200700027
Links to Exploration step
ISTEX:5BAFE9301BDD14B0C63EA4BEAEAC69564E0B6E0DLe document en format XML
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KGaA, Weinheim</copyright><eventGroup><event type="manuscriptReceived" date="2007-02-02"></event><event type="manuscriptRevised" date="2007-04-09"></event><event type="manuscriptAccepted" date="2007-04-10"></event><event type="firstOnline" date="2007-05-25"></event><event type="publishedOnlineFinalForm" date="2007-07-06"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-07"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst">879</numbering><numbering type="pageLast">885</numbering></numberingGroup><correspondenceTo>Lavrentyev pr. 8, Novosibirsk, 630090, Russia, <b>Fax:</b> +7‐3833‐333677</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:BIOT.BIOT200700027.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="30"></count></countGroup><titleGroup><title type="main" xml:lang="en">Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Asya</givenNames><familyName>Levina</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Inna</givenNames><familyName>Pyshnaya</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Marina</givenNames><familyName>Repkova</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#a1"><personName><givenNames>Vera</givenNames><familyName>Rar</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#a1" corresponding="yes"><personName><givenNames>Valentina</givenNames><familyName>Zarytova</familyName><degrees>Professor</degrees></personName><contactDetails><email>Zarytova@niboch.nsc.ru</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="RU" type="organization"><unparsedAffiliation>Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">DNA chips</keyword><keyword xml:id="kwd2">Polyamine‐oligonucleotide</keyword><keyword xml:id="kwd3">Immobilization</keyword></keywordGroup><fundingInfo><fundingAgency>INCO</fundingAgency><fundingNumber>IC15‐CT98‐03039</fundingNumber></fundingInfo><fundingInfo><fundingAgency>SB RAS Integration</fundingAgency><fundingNumber>126</fundingNumber><fundingNumber>73</fundingNumber></fundingInfo><fundingInfo><fundingAgency>RFBR</fundingAgency><fundingNumber>05‐04‐08040‐ofi‐a</fundingNumber></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Various materials, such as glass, plastic, metals, etc., are utilized for preparing DNA chips. In each particular case special approaches are used for immobilization of different oligonucleotide derivatives on the solid supports. We describe a general technique for DNA chips preparation on various unmodified surfaces using one type of oligonucleotide derivative, polylysine‐oligonucleotide conjugates (PL‐oligo). A long polyamine spacer in the PL‐oligo conjugates provides a durable irreversible non‐covalent immobilization onto a variety of solid supports and enough distance between oligonucleotides and the surface. The resulting DNA chips were shown to be useful for the detection of PCR DNA fragments and to be sensitive to single nucleotide discrepancies. They represent a promising instrument for revealing genetic diseases, genotyping viruses and bacteria, and for displaying their drug‐resistant strains.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces</title></titleInfo><name type="personal"><namePart type="given">Asya</namePart><namePart type="family">Levina</namePart><affiliation>Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Inna</namePart><namePart type="family">Pyshnaya</namePart><affiliation>Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marina</namePart><namePart type="family">Repkova</namePart><affiliation>Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Vera</namePart><namePart type="family">Rar</namePart><affiliation>Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Valentina</namePart><namePart type="family">Zarytova</namePart><namePart type="termsOfAddress">Professor</namePart><affiliation>Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia</affiliation><affiliation>E-mail: Zarytova@niboch.nsc.ru</affiliation><affiliation>Correspondence address: Lavrentyev pr. 8, Novosibirsk, 630090, Russia, +7‐3833‐333677</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Weinheim</placeTerm></place><dateIssued encoding="w3cdtf">2007-07</dateIssued><dateCaptured encoding="w3cdtf">2007-02-02</dateCaptured><dateValid encoding="w3cdtf">2007-04-10</dateValid><copyrightDate encoding="w3cdtf">2007</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">5</extent><extent unit="tables">1</extent><extent unit="references">30</extent></physicalDescription><abstract lang="en">Various materials, such as glass, plastic, metals, etc., are utilized for preparing DNA chips. In each particular case special approaches are used for immobilization of different oligonucleotide derivatives on the solid supports. We describe a general technique for DNA chips preparation on various unmodified surfaces using one type of oligonucleotide derivative, polylysine‐oligonucleotide conjugates (PL‐oligo). A long polyamine spacer in the PL‐oligo conjugates provides a durable irreversible non‐covalent immobilization onto a variety of solid supports and enough distance between oligonucleotides and the surface. The resulting DNA chips were shown to be useful for the detection of PCR DNA fragments and to be sensitive to single nucleotide discrepancies. They represent a promising instrument for revealing genetic diseases, genotyping viruses and bacteria, and for displaying their drug‐resistant strains.</abstract><note type="funding">INCO - No. IC15‐CT98‐03039; </note><note type="funding">SB RAS Integration - No. 126; No. 73; </note><note type="funding">RFBR - No. 05‐04‐08040‐ofi‐a; </note><subject lang="en"><genre>keywords</genre><topic>DNA chips</topic><topic>Polyamine‐oligonucleotide</topic><topic>Immobilization</topic></subject><relatedItem type="host"><titleInfo><title>Biotechnology Journal</title><subTitle>Healthcare Nutrition Technology</subTitle></titleInfo><titleInfo type="abbreviated"><title>Biotechnology Journal</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic><topic>Research Articles</topic></subject><identifier type="ISSN">1860-6768</identifier><identifier type="eISSN">1860-7314</identifier><identifier type="DOI">10.1002/(ISSN)1860-7314</identifier><identifier type="PublisherID">BIOT</identifier><part><date>2007</date><detail type="title"><title>Biotech in Russia</title></detail><detail type="volume"><caption>vol.</caption><number>2</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>879</start><end>885</end><total>7</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>Affinity sorbents containing nucleic acids and their fragments.</title></titleInfo><name type="personal"><namePart type="given">I. 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