Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase
Identifieur interne : 001134 ( Istex/Corpus ); précédent : 001133; suivant : 001135Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase
Auteurs : Hernando Duque ; Ann C. PalmenbergSource :
- Virus Genes [ 0920-8569 ] ; 1996-01-01.
English descriptors
- KwdEn :
- Teeft :
- Antibody, Antibody binding, Antibody samples, Ascitic fluid, Cell extracts, Convalescent animals, Different epitopes, Duqueand palmenberg, Elisa, Epitope, Epitope mapping, Fmdv, Horseradish peroxidase, Inclusion bodies, Mabs, Mengo, Molecular mass, Monoclonal, Monoclonal antibodies, Native protein, Natural mengo, Other antibodies, Other hand, Other picornaviruses, Other viruses, Palmenberg, Picornavirus, Polymerase, Recombinant, Recombinant mengo, Rm3d, Rm3d mabs, Rm3d protein, Sodium deoxycholate, Strong reactivity, Urea, Vaccinated animals, Vaccine, Viral, Weak reactivity, Western analysis, Western blots.
Abstract
Abstract: The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol (rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpol in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpol from poliovirus type 1 and 3 or rhinovirus type 14 and 16.
Url:
DOI: 10.1007/BF00568908
Links to Exploration step
ISTEX:C268589417FDBA670305090FC9E7EA4610B36647Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase</title>
<author><name sortKey="Duque, Hernando" sort="Duque, Hernando" uniqKey="Duque H" first="Hernando" last="Duque">Hernando Duque</name>
<affiliation><mods:affiliation>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Palmenberg, Ann C" sort="Palmenberg, Ann C" uniqKey="Palmenberg A" first="Ann C." last="Palmenberg">Ann C. Palmenberg</name>
<affiliation><mods:affiliation>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:C268589417FDBA670305090FC9E7EA4610B36647</idno>
<date when="1996" year="1996">1996</date>
<idno type="doi">10.1007/BF00568908</idno>
<idno type="url">https://api.istex.fr/ark:/67375/1BB-NDD1GGVD-Q/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001134</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001134</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase</title>
<author><name sortKey="Duque, Hernando" sort="Duque, Hernando" uniqKey="Duque H" first="Hernando" last="Duque">Hernando Duque</name>
<affiliation><mods:affiliation>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Palmenberg, Ann C" sort="Palmenberg, Ann C" uniqKey="Palmenberg A" first="Ann C." last="Palmenberg">Ann C. Palmenberg</name>
<affiliation><mods:affiliation>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virus Genes</title>
<title level="j" type="abbrev">Virus Genes</title>
<idno type="ISSN">0920-8569</idno>
<idno type="eISSN">1572-994X</idno>
<imprint><publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="1996-01-01">1996-01-01</date>
<biblScope unit="volume">13</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="159">159</biblScope>
<biblScope unit="page" to="168">168</biblScope>
</imprint>
<idno type="ISSN">0920-8569</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0920-8569</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>3D polymerase</term>
<term>Mengo virus</term>
<term>mapping</term>
<term>monoclonal antibody</term>
<term>picornavirus</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Antibody</term>
<term>Antibody binding</term>
<term>Antibody samples</term>
<term>Ascitic fluid</term>
<term>Cell extracts</term>
<term>Convalescent animals</term>
<term>Different epitopes</term>
<term>Duqueand palmenberg</term>
<term>Elisa</term>
<term>Epitope</term>
<term>Epitope mapping</term>
<term>Fmdv</term>
<term>Horseradish peroxidase</term>
<term>Inclusion bodies</term>
<term>Mabs</term>
<term>Mengo</term>
<term>Molecular mass</term>
<term>Monoclonal</term>
<term>Monoclonal antibodies</term>
<term>Native protein</term>
<term>Natural mengo</term>
<term>Other antibodies</term>
<term>Other hand</term>
<term>Other picornaviruses</term>
<term>Other viruses</term>
<term>Palmenberg</term>
<term>Picornavirus</term>
<term>Polymerase</term>
<term>Recombinant</term>
<term>Recombinant mengo</term>
<term>Rm3d</term>
<term>Rm3d mabs</term>
<term>Rm3d protein</term>
<term>Sodium deoxycholate</term>
<term>Strong reactivity</term>
<term>Urea</term>
<term>Vaccinated animals</term>
<term>Vaccine</term>
<term>Viral</term>
<term>Weak reactivity</term>
<term>Western analysis</term>
<term>Western blots</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol (rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpol in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpol from poliovirus type 1 and 3 or rhinovirus type 14 and 16.</div>
</front>
</TEI>
<istex><corpusName>springer-journals</corpusName>
<keywords><teeft><json:string>epitope</json:string>
<json:string>mengo</json:string>
<json:string>rm3d</json:string>
<json:string>mabs</json:string>
<json:string>recombinant</json:string>
<json:string>palmenberg</json:string>
<json:string>picornavirus</json:string>
<json:string>viral</json:string>
<json:string>monoclonal</json:string>
<json:string>recombinant mengo</json:string>
<json:string>polymerase</json:string>
<json:string>fmdv</json:string>
<json:string>elisa</json:string>
<json:string>vaccine</json:string>
<json:string>monoclonal antibodies</json:string>
<json:string>urea</json:string>
<json:string>western blots</json:string>
<json:string>sodium deoxycholate</json:string>
<json:string>horseradish peroxidase</json:string>
<json:string>cell extracts</json:string>
<json:string>strong reactivity</json:string>
<json:string>epitope mapping</json:string>
<json:string>antibody binding</json:string>
<json:string>vaccinated animals</json:string>
<json:string>inclusion bodies</json:string>
<json:string>convalescent animals</json:string>
<json:string>different epitopes</json:string>
<json:string>rm3d mabs</json:string>
<json:string>antibody samples</json:string>
<json:string>ascitic fluid</json:string>
<json:string>natural mengo</json:string>
<json:string>duqueand palmenberg</json:string>
<json:string>rm3d protein</json:string>
<json:string>western analysis</json:string>
<json:string>other hand</json:string>
<json:string>native protein</json:string>
<json:string>other antibodies</json:string>
<json:string>weak reactivity</json:string>
<json:string>other picornaviruses</json:string>
<json:string>molecular mass</json:string>
<json:string>other viruses</json:string>
<json:string>antibody</json:string>
</teeft>
</keywords>
<author><json:item><name>Hernando Duque</name>
<affiliations><json:string>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</json:string>
<json:string>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</json:string>
</affiliations>
</json:item>
<json:item><name>Ann C. Palmenberg</name>
<affiliations><json:string>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</json:string>
<json:string>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</json:string>
</affiliations>
</json:item>
</author>
<subject><json:item><lang><json:string>eng</json:string>
</lang>
<value>picornavirus</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Mengo virus</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>3D polymerase</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>mapping</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>monoclonal antibody</value>
</json:item>
</subject>
<articleId><json:string>BF00568908</json:string>
<json:string>Art6</json:string>
</articleId>
<arkIstex>ark:/67375/1BB-NDD1GGVD-Q</arkIstex>
<language><json:string>eng</json:string>
</language>
<originalGenre><json:string>OriginalPaper</json:string>
</originalGenre>
<abstract>Abstract: The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol (rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpol in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpol from poliovirus type 1 and 3 or rhinovirus type 14 and 16.</abstract>
<qualityIndicators><refBibsNative>false</refBibsNative>
<abstractWordCount>106</abstractWordCount>
<abstractCharCount>682</abstractCharCount>
<keywordCount>5</keywordCount>
<score>7.923</score>
<pdfWordCount>4651</pdfWordCount>
<pdfCharCount>27579</pdfCharCount>
<pdfVersion>1.3</pdfVersion>
<pdfPageCount>10</pdfPageCount>
<pdfPageSize>555 x 738 pts</pdfPageSize>
</qualityIndicators>
<title>Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase</title>
<pmid><json:string>8972569</json:string>
</pmid>
<genre><json:string>research-article</json:string>
</genre>
<host><title>Virus Genes</title>
<language><json:string>unknown</json:string>
</language>
<publicationDate>1996</publicationDate>
<copyrightDate>1996</copyrightDate>
<issn><json:string>0920-8569</json:string>
</issn>
<eissn><json:string>1572-994X</json:string>
</eissn>
<journalId><json:string>11262</json:string>
</journalId>
<volume>13</volume>
<issue>2</issue>
<pages><first>159</first>
<last>168</last>
</pages>
<genre><json:string>journal</json:string>
</genre>
<subject><json:item><value>Medical Microbiology</value>
</json:item>
<json:item><value>Virology</value>
</json:item>
<json:item><value>Plant Sciences</value>
</json:item>
</subject>
</host>
<namedEntities><unitex><date></date>
<geogName></geogName>
<orgName></orgName>
<orgName_funder></orgName_funder>
<orgName_provider></orgName_provider>
<persName></persName>
<placeName></placeName>
<ref_url></ref_url>
<ref_bibl></ref_bibl>
<bibl></bibl>
</unitex>
</namedEntities>
<ark><json:string>ark:/67375/1BB-NDD1GGVD-Q</json:string>
</ark>
<categories><wos><json:string>1 - science</json:string>
<json:string>2 - virology</json:string>
<json:string>2 - genetics & heredity</json:string>
</wos>
<scienceMetrix><json:string>1 - health sciences</json:string>
<json:string>2 - biomedical research</json:string>
<json:string>3 - virology</json:string>
</scienceMetrix>
<scopus><json:string>1 - Life Sciences</json:string>
<json:string>2 - Immunology and Microbiology</json:string>
<json:string>3 - Virology</json:string>
<json:string>1 - Life Sciences</json:string>
<json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string>
<json:string>3 - Genetics</json:string>
<json:string>1 - Life Sciences</json:string>
<json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string>
<json:string>3 - Molecular Biology</json:string>
<json:string>1 - Health Sciences</json:string>
<json:string>2 - Medicine</json:string>
<json:string>3 - General Medicine</json:string>
</scopus>
<inist><json:string>1 - sciences appliquees, technologies et medecines</json:string>
<json:string>2 - sciences biologiques et medicales</json:string>
<json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string>
<json:string>4 - psychologie. psychophysiologie</json:string>
</inist>
</categories>
<publicationDate>1996</publicationDate>
<copyrightDate>1996</copyrightDate>
<doi><json:string>10.1007/BF00568908</json:string>
</doi>
<id>C268589417FDBA670305090FC9E7EA4610B36647</id>
<score>1</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/ark:/67375/1BB-NDD1GGVD-Q/fulltext.pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/ark:/67375/1BB-NDD1GGVD-Q/bundle.zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/1BB-NDD1GGVD-Q/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher scheme="https://scientific-publisher.data.istex.fr">Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<availability><licence><p>Kluwer Academic Publishers, 1996</p>
</licence>
<p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p>
</availability>
<date>1996-06-12</date>
</publicationStmt>
<notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note>
<note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note>
</notesStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase</title>
<author xml:id="author-0000"><persName><forename type="first">Hernando</forename>
<surname>Duque</surname>
</persName>
<affiliation>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</affiliation>
<affiliation>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</affiliation>
</author>
<author xml:id="author-0001"><persName><forename type="first">Ann</forename>
<surname>Palmenberg</surname>
</persName>
<affiliation>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</affiliation>
<affiliation>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</affiliation>
</author>
<idno type="istex">C268589417FDBA670305090FC9E7EA4610B36647</idno>
<idno type="ark">ark:/67375/1BB-NDD1GGVD-Q</idno>
<idno type="DOI">10.1007/BF00568908</idno>
<idno type="article-id">BF00568908</idno>
<idno type="article-id">Art6</idno>
</analytic>
<monogr><title level="j">Virus Genes</title>
<title level="j" type="abbrev">Virus Genes</title>
<idno type="pISSN">0920-8569</idno>
<idno type="eISSN">1572-994X</idno>
<idno type="journal-ID">true</idno>
<idno type="issue-article-count">9</idno>
<idno type="volume-issue-count">3</idno>
<imprint><publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="1996-01-01"></date>
<biblScope unit="volume">13</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="159">159</biblScope>
<biblScope unit="page" to="168">168</biblScope>
</imprint>
</monogr>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>1996-06-12</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>Abstract: The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol (rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpol in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpol from poliovirus type 1 and 3 or rhinovirus type 14 and 16.</p>
</abstract>
<textClass xml:lang="en"><keywords scheme="keyword"><list><head>Key words</head>
<item><term>picornavirus</term>
</item>
<item><term>Mengo virus</term>
</item>
<item><term>3D polymerase</term>
</item>
<item><term>mapping</term>
</item>
<item><term>monoclonal antibody</term>
</item>
</list>
</keywords>
</textClass>
<textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head>
<item><term>Medical Microbiology</term>
</item>
<item><term>Virology</term>
</item>
<item><term>Plant Sciences</term>
</item>
</list>
</keywords>
</textClass>
</profileDesc>
<revisionDesc><change when="1996-06-12">Created</change>
<change when="1996-01-01">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/ark:/67375/1BB-NDD1GGVD-Q/fulltext.txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType>
<istex:document><Publisher><PublisherInfo><PublisherName>Kluwer Academic Publishers</PublisherName>
<PublisherLocation>Dordrecht</PublisherLocation>
</PublisherInfo>
<Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11262</JournalID>
<JournalPrintISSN>0920-8569</JournalPrintISSN>
<JournalElectronicISSN>1572-994X</JournalElectronicISSN>
<JournalTitle>Virus Genes</JournalTitle>
<JournalAbbreviatedTitle>Virus Genes</JournalAbbreviatedTitle>
<JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject>
<JournalSubject Type="Secondary">Medical Microbiology</JournalSubject>
<JournalSubject Type="Secondary">Virology</JournalSubject>
<JournalSubject Type="Secondary">Plant Sciences</JournalSubject>
</JournalSubjectGroup>
</JournalInfo>
<Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>13</VolumeIDStart>
<VolumeIDEnd>13</VolumeIDEnd>
<VolumeIssueCount>3</VolumeIssueCount>
</VolumeInfo>
<Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>2</IssueIDStart>
<IssueIDEnd>2</IssueIDEnd>
<IssueArticleCount>9</IssueArticleCount>
<IssueHistory><CoverDate><DateString>1996</DateString>
<Year>1996</Year>
<Month>1</Month>
</CoverDate>
</IssueHistory>
<IssueCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName>
<CopyrightYear>1996</CopyrightYear>
</IssueCopyright>
</IssueInfo>
<Article ID="Art6"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00568908</ArticleID>
<ArticleDOI>10.1007/BF00568908</ArticleDOI>
<ArticleSequenceNumber>6</ArticleSequenceNumber>
<ArticleTitle Language="En">Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase</ArticleTitle>
<ArticleFirstPage>159</ArticleFirstPage>
<ArticleLastPage>168</ArticleLastPage>
<ArticleHistory><RegistrationDate><Year>2004</Year>
<Month>10</Month>
<Day>16</Day>
</RegistrationDate>
<Received><Year>1996</Year>
<Month>6</Month>
<Day>12</Day>
</Received>
<Accepted><Year>1996</Year>
<Month>6</Month>
<Day>12</Day>
</Accepted>
</ArticleHistory>
<ArticleCopyright><CopyrightHolderName>Kluwer Academic Publishers</CopyrightHolderName>
<CopyrightYear>1996</CopyrightYear>
</ArticleCopyright>
<ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant>
<AbstractGrant Grant="OpenAccess"></AbstractGrant>
<BodyPDFGrant Grant="Restricted"></BodyPDFGrant>
<BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant>
<BibliographyGrant Grant="Restricted"></BibliographyGrant>
<ESMGrant Grant="Restricted"></ESMGrant>
</ArticleGrants>
<ArticleContext><JournalID>11262</JournalID>
<VolumeIDStart>13</VolumeIDStart>
<VolumeIDEnd>13</VolumeIDEnd>
<IssueIDStart>2</IssueIDStart>
<IssueIDEnd>2</IssueIDEnd>
</ArticleContext>
</ArticleInfo>
<ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Hernando</GivenName>
<FamilyName>Duque</FamilyName>
</AuthorName>
</Author>
<Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Ann</GivenName>
<GivenName>C.</GivenName>
<FamilyName>Palmenberg</FamilyName>
</AuthorName>
</Author>
<Affiliation ID="Aff1"><OrgDivision>Institute for Molecular Virology</OrgDivision>
<OrgName>University of Wisconsin-Madison</OrgName>
<OrgAddress><Street>1655 Linden Drive</Street>
<Postcode>53706</Postcode>
<City>Madison</City>
<State>Wisconsin</State>
<Country>USA</Country>
</OrgAddress>
</Affiliation>
<Affiliation ID="Aff2"><OrgDivision>Department of Animal Health & Biomedical Sciences</OrgDivision>
<OrgName>University of Wisconsin-Madison</OrgName>
<OrgAddress><Street>1655 Linden Drive</Street>
<Postcode>53706</Postcode>
<City>Madison</City>
<State>Wisconsin</State>
<Country>USA</Country>
</OrgAddress>
</Affiliation>
</AuthorGroup>
<Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading>
<Para>The cDNA coding sequence of the RNA-dependent RNA polymerase (3D<Superscript>pol</Superscript>
) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3D<Superscript>pol</Superscript>
(rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3D<Superscript>pol</Superscript>
in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3D<Superscript>pol</Superscript>
from poliovirus type 1 and 3 or rhinovirus type 14 and 16.</Para>
</Abstract>
<KeywordGroup Language="En"><Heading>Key words</Heading>
<Keyword>picornavirus</Keyword>
<Keyword>Mengo virus</Keyword>
<Keyword>3D polymerase</Keyword>
<Keyword>mapping</Keyword>
<Keyword>monoclonal antibody</Keyword>
</KeywordGroup>
</ArticleHeader>
<NoBody></NoBody>
</Article>
</Issue>
</Volume>
</Journal>
</Publisher>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo lang="en"><title>Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA"><title>Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase</title>
</titleInfo>
<name type="personal"><namePart type="given">Hernando</namePart>
<namePart type="family">Duque</namePart>
<affiliation>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</affiliation>
<affiliation>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Ann</namePart>
<namePart type="given">C.</namePart>
<namePart type="family">Palmenberg</namePart>
<affiliation>Institute for Molecular Virology, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</affiliation>
<affiliation>Department of Animal Health & Biomedical Sciences, University of Wisconsin-Madison, 1655 Linden Drive, 53706, Madison, Wisconsin, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre>
<originInfo><publisher>Kluwer Academic Publishers</publisher>
<place><placeTerm type="text">Dordrecht</placeTerm>
</place>
<dateCreated encoding="w3cdtf">1996-06-12</dateCreated>
<dateIssued encoding="w3cdtf">1996-01-01</dateIssued>
<copyrightDate encoding="w3cdtf">1996</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
</language>
<abstract lang="en">Abstract: The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol (rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpol in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpol from poliovirus type 1 and 3 or rhinovirus type 14 and 16.</abstract>
<subject lang="en"><genre>Key words</genre>
<topic>picornavirus</topic>
<topic>Mengo virus</topic>
<topic>3D polymerase</topic>
<topic>mapping</topic>
<topic>monoclonal antibody</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Virus Genes</title>
</titleInfo>
<titleInfo type="abbreviated"><title>Virus Genes</title>
</titleInfo>
<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<originInfo><publisher>Springer</publisher>
<dateIssued encoding="w3cdtf">1996-01-01</dateIssued>
<copyrightDate encoding="w3cdtf">1996</copyrightDate>
</originInfo>
<subject><genre>Biomedicine</genre>
<topic>Medical Microbiology</topic>
<topic>Virology</topic>
<topic>Plant Sciences</topic>
</subject>
<identifier type="ISSN">0920-8569</identifier>
<identifier type="eISSN">1572-994X</identifier>
<identifier type="JournalID">11262</identifier>
<identifier type="IssueArticleCount">9</identifier>
<identifier type="VolumeIssueCount">3</identifier>
<part><date>1996</date>
<detail type="volume"><number>13</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>2</number>
<caption>no.</caption>
</detail>
<extent unit="pages"><start>159</start>
<end>168</end>
</extent>
</part>
<recordInfo><recordOrigin>Kluwer Academic Publishers, 1996</recordOrigin>
</recordInfo>
</relatedItem>
<identifier type="istex">C268589417FDBA670305090FC9E7EA4610B36647</identifier>
<identifier type="ark">ark:/67375/1BB-NDD1GGVD-Q</identifier>
<identifier type="DOI">10.1007/BF00568908</identifier>
<identifier type="ArticleID">BF00568908</identifier>
<identifier type="ArticleID">Art6</identifier>
<accessCondition type="use and reproduction" contentType="copyright">Kluwer Academic Publishers, 1996</accessCondition>
<recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource>
<recordOrigin>Kluwer Academic Publishers, 1996</recordOrigin>
</recordInfo>
</mods>
<json:item><extension>json</extension>
<original>false</original>
<mimetype>application/json</mimetype>
<uri>https://api.istex.fr/ark:/67375/1BB-NDD1GGVD-Q/record.json</uri>
</json:item>
</metadata>
<serie></serie>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001134 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001134 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:C268589417FDBA670305090FC9E7EA4610B36647 |texte= Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |