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Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase

Identifieur interne : 001134 ( Istex/Corpus ); précédent : 001133; suivant : 001135

Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase

Auteurs : Hernando Duque ; Ann C. Palmenberg

Source :

RBID : ISTEX:C268589417FDBA670305090FC9E7EA4610B36647

English descriptors

Abstract

Abstract: The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol (rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpol in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpol from poliovirus type 1 and 3 or rhinovirus type 14 and 16.

Url:
DOI: 10.1007/BF00568908

Links to Exploration step

ISTEX:C268589417FDBA670305090FC9E7EA4610B36647

Le document en format XML

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<Superscript>pol</Superscript>
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<abstract lang="en">Abstract: The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol (rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpol in western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpol from poliovirus type 1 and 3 or rhinovirus type 14 and 16.</abstract>
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