Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: A Model of Protective Immunity to HIV
Identifieur interne : 001098 ( Istex/Corpus ); précédent : 001097; suivant : 001099Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: A Model of Protective Immunity to HIV
Auteurs : Jennifer L. Kelschenbach ; Manisha Saini ; Eran Hadas ; Chao-Jiang Gu ; Wei Chao ; Galina Bentsman ; Jessie P. Hong ; Tomas Hanke ; Leroy R. Sharer ; Mary Jane Potash ; David J. VolskySource :
- Journal of Neuroimmune Pharmacology [ 1557-1890 ] ; 2012-06-01.
English descriptors
- KwdEn :
Abstract
Abstract: Infection by some viruses induces immunity to reinfection, providing a means to identify protective epitopes. To investigate resistance to reinfection in an animal model of HIV disease and its control, we employed infection of mice with chimeric HIV, EcoHIV. When immunocompetent mice were infected by intraperitoneal (IP) injection of EcoHIV, they resisted subsequent secondary infection by IP injection, consistent with a systemic antiviral immune response. To investigate the potential role of these responses in restricting neurotropic HIV infection, we established a protocol for efficient EcoHIV expression in the brain following intracranial (IC) inoculation of virus. When mice were inoculated by IP injection and secondarily by IC injection, they also controlled EcoHIV replication in the brain. To investigate their role in EcoHIV antiviral responses, CD8+ T lymphocytes were isolated from spleens of EcoHIV infected and uninfected mice and adoptively transferred to isogenic recipients. Recipients of EcoHIV primed CD8+ cells resisted subsequent EcoHIV infection compared to recipients of cells from uninfected donors. CD8+ spleen cells from EcoHIV-infected mice also mounted modest but significant interferon-γ responses to two HIV Gag peptide pools. These findings suggest EcoHIV-infected mice may serve as a useful system to investigate the induction of anti-HIV protective immunity for eventual translation to human beings.
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DOI: 10.1007/s11481-011-9316-1
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Kelschenbach</name><affiliation><mods:affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: jlk2152@columbia.edu</mods:affiliation></affiliation></author><author><name sortKey="Saini, Manisha" sort="Saini, Manisha" uniqKey="Saini M" first="Manisha" last="Saini">Manisha Saini</name><affiliation><mods:affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: manisaini@gmail.com</mods:affiliation></affiliation></author><author><name sortKey="Hadas, Eran" sort="Hadas, Eran" uniqKey="Hadas E" first="Eran" last="Hadas">Eran Hadas</name><affiliation><mods:affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: eran.hadas@gmail.com</mods:affiliation></affiliation></author><author><name sortKey="Gu, Chao Jiang" sort="Gu, Chao Jiang" uniqKey="Gu C" first="Chao-Jiang" last="Gu">Chao-Jiang Gu</name><affiliation><mods:affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: cg2447@columbia.edu</mods:affiliation></affiliation></author><author><name sortKey="Chao, Wei" sort="Chao, Wei" uniqKey="Chao W" first="Wei" last="Chao">Wei Chao</name><affiliation><mods:affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: wei616@verizon.net</mods:affiliation></affiliation></author><author><name sortKey="Bentsman, Galina" sort="Bentsman, Galina" uniqKey="Bentsman G" first="Galina" last="Bentsman">Galina Bentsman</name><affiliation><mods:affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: galina_bentsman@yahoo.com</mods:affiliation></affiliation></author><author><name sortKey="Hong, Jessie P" sort="Hong, Jessie P" uniqKey="Hong J" first="Jessie P." last="Hong">Jessie P. Hong</name><affiliation><mods:affiliation>Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, OX3 9DS, Oxford, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: jessie.hong@gmail.com</mods:affiliation></affiliation></author><author><name sortKey="Hanke, Tomas" sort="Hanke, Tomas" uniqKey="Hanke T" first="Tomas" last="Hanke">Tomas Hanke</name><affiliation><mods:affiliation>Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, OX3 9DS, Oxford, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: tomas.hanke@molecular-medicine.oxford.ac.uk</mods:affiliation></affiliation></author><author><name sortKey="Sharer, Leroy R" sort="Sharer, Leroy R" uniqKey="Sharer L" first="Leroy R." last="Sharer">Leroy R. Sharer</name><affiliation><mods:affiliation>Department of Pathology, New Jersey Medical School, 07103, Newark, NJ, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: sharer@umdnj.edu</mods:affiliation></affiliation></author><author><name sortKey="Potash, Mary Jane" sort="Potash, Mary Jane" uniqKey="Potash M" first="Mary Jane" last="Potash">Mary Jane Potash</name><affiliation><mods:affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</mods:affiliation></affiliation></author><author><name sortKey="Volsky, David J" sort="Volsky, David J" uniqKey="Volsky D" first="David J." last="Volsky">David J. Volsky</name><affiliation><mods:affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: djv4@columbia.edu</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Neuroimmune Pharmacology</title><title level="j" type="abbrev">J Neuroimmune Pharmacol</title><idno type="ISSN">1557-1890</idno><idno type="eISSN">1557-1904</idno><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2012-06-01">2012-06-01</date><biblScope unit="volume">7</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="380">380</biblScope><biblScope unit="page" to="387">387</biblScope></imprint><idno type="ISSN">1557-1890</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1557-1890</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptive immune responses</term><term>Brain</term><term>Chimeric HIV</term><term>HIV neuropathogenesis</term><term>Mouse model</term><term>Superinfection</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Infection by some viruses induces immunity to reinfection, providing a means to identify protective epitopes. To investigate resistance to reinfection in an animal model of HIV disease and its control, we employed infection of mice with chimeric HIV, EcoHIV. When immunocompetent mice were infected by intraperitoneal (IP) injection of EcoHIV, they resisted subsequent secondary infection by IP injection, consistent with a systemic antiviral immune response. To investigate the potential role of these responses in restricting neurotropic HIV infection, we established a protocol for efficient EcoHIV expression in the brain following intracranial (IC) inoculation of virus. When mice were inoculated by IP injection and secondarily by IC injection, they also controlled EcoHIV replication in the brain. To investigate their role in EcoHIV antiviral responses, CD8+ T lymphocytes were isolated from spleens of EcoHIV infected and uninfected mice and adoptively transferred to isogenic recipients. Recipients of EcoHIV primed CD8+ cells resisted subsequent EcoHIV infection compared to recipients of cells from uninfected donors. CD8+ spleen cells from EcoHIV-infected mice also mounted modest but significant interferon-γ responses to two HIV Gag peptide pools. These findings suggest EcoHIV-infected mice may serve as a useful system to investigate the induction of anti-HIV protective immunity for eventual translation to human beings.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Jennifer L. Kelschenbach</name><affiliations><json:string>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</json:string><json:string>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</json:string><json:string>E-mail: jlk2152@columbia.edu</json:string></affiliations></json:item><json:item><name>Manisha Saini</name><affiliations><json:string>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</json:string><json:string>E-mail: manisaini@gmail.com</json:string></affiliations></json:item><json:item><name>Eran Hadas</name><affiliations><json:string>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</json:string><json:string>E-mail: eran.hadas@gmail.com</json:string></affiliations></json:item><json:item><name>Chao-jiang Gu</name><affiliations><json:string>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</json:string><json:string>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</json:string><json:string>E-mail: cg2447@columbia.edu</json:string></affiliations></json:item><json:item><name>Wei Chao</name><affiliations><json:string>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</json:string><json:string>E-mail: wei616@verizon.net</json:string></affiliations></json:item><json:item><name>Galina Bentsman</name><affiliations><json:string>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</json:string><json:string>E-mail: galina_bentsman@yahoo.com</json:string></affiliations></json:item><json:item><name>Jessie P. Hong</name><affiliations><json:string>Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, OX3 9DS, Oxford, UK</json:string><json:string>E-mail: jessie.hong@gmail.com</json:string></affiliations></json:item><json:item><name>Tomas Hanke</name><affiliations><json:string>Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, OX3 9DS, Oxford, UK</json:string><json:string>E-mail: tomas.hanke@molecular-medicine.oxford.ac.uk</json:string></affiliations></json:item><json:item><name>Leroy R. Sharer</name><affiliations><json:string>Department of Pathology, New Jersey Medical School, 07103, Newark, NJ, USA</json:string><json:string>E-mail: sharer@umdnj.edu</json:string></affiliations></json:item><json:item><name>Mary Jane Potash</name><affiliations><json:string>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</json:string><json:string>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</json:string></affiliations></json:item><json:item><name>David J. Volsky</name><affiliations><json:string>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</json:string><json:string>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</json:string><json:string>E-mail: djv4@columbia.edu</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Mouse model</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Chimeric HIV</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Superinfection</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Adaptive immune responses</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>HIV neuropathogenesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Brain</value></json:item></subject><articleId><json:string>9316</json:string><json:string>s11481-011-9316-1</json:string></articleId><arkIstex>ark:/67375/VQC-VNSFD39P-R</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: Infection by some viruses induces immunity to reinfection, providing a means to identify protective epitopes. To investigate resistance to reinfection in an animal model of HIV disease and its control, we employed infection of mice with chimeric HIV, EcoHIV. When immunocompetent mice were infected by intraperitoneal (IP) injection of EcoHIV, they resisted subsequent secondary infection by IP injection, consistent with a systemic antiviral immune response. To investigate the potential role of these responses in restricting neurotropic HIV infection, we established a protocol for efficient EcoHIV expression in the brain following intracranial (IC) inoculation of virus. When mice were inoculated by IP injection and secondarily by IC injection, they also controlled EcoHIV replication in the brain. To investigate their role in EcoHIV antiviral responses, CD8+ T lymphocytes were isolated from spleens of EcoHIV infected and uninfected mice and adoptively transferred to isogenic recipients. Recipients of EcoHIV primed CD8+ cells resisted subsequent EcoHIV infection compared to recipients of cells from uninfected donors. CD8+ spleen cells from EcoHIV-infected mice also mounted modest but significant interferon-γ responses to two HIV Gag peptide pools. These findings suggest EcoHIV-infected mice may serve as a useful system to investigate the induction of anti-HIV protective immunity for eventual translation to human beings.</abstract><qualityIndicators><score>8.697</score><pdfWordCount>4237</pdfWordCount><pdfCharCount>26890</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>595.276 x 790.866 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>205</abstractWordCount><abstractCharCount>1448</abstractCharCount><keywordCount>6</keywordCount></qualityIndicators><title>Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: A Model of Protective Immunity to HIV</title><genre><json:string>research-article</json:string></genre><host><title>Journal of Neuroimmune Pharmacology</title><language><json:string>unknown</json:string></language><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><issn><json:string>1557-1890</json:string></issn><eissn><json:string>1557-1904</json:string></eissn><journalId><json:string>11481</json:string></journalId><volume>7</volume><issue>2</issue><pages><first>380</first><last>387</last></pages><genre><json:string>journal</json:string></genre><editor><json:item><name>Howard Fox</name></json:item><json:item><name>Howard E. Gendelman</name></json:item></editor><subject><json:item><value>Virology</value></json:item><json:item><value>Pharmacology/Toxicology</value></json:item><json:item><value>Cell Biology</value></json:item><json:item><value>Immunology</value></json:item><json:item><value>Neurosciences</value></json:item></subject></host><ark><json:string>ark:/67375/VQC-VNSFD39P-R</json:string></ark><publicationDate>2012</publicationDate><copyrightDate>2011</copyrightDate><doi><json:string>10.1007/s11481-011-9316-1</json:string></doi><id>4965E275D19BC1D93D36047D12435B604366B022</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-VNSFD39P-R/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-VNSFD39P-R/bundle.zip</uri></json:item><istex:fulltextTEI 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ARTICLE</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: A Model of Protective Immunity to HIV</title><author xml:id="author-0000"><persName><forename type="first">Jennifer</forename><surname>Kelschenbach</surname></persName><email>jlk2152@columbia.edu</email><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Manisha</forename><surname>Saini</surname></persName><email>manisaini@gmail.com</email><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Eran</forename><surname>Hadas</surname></persName><email>eran.hadas@gmail.com</email><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Chao-jiang</forename><surname>Gu</surname></persName><email>cg2447@columbia.edu</email><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Wei</forename><surname>Chao</surname></persName><email>wei616@verizon.net</email><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Galina</forename><surname>Bentsman</surname></persName><email>galina_bentsman@yahoo.com</email><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation></author><author xml:id="author-0006"><persName><forename type="first">Jessie</forename><surname>Hong</surname></persName><email>jessie.hong@gmail.com</email><affiliation>Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, OX3 9DS, Oxford, UK</affiliation></author><author xml:id="author-0007"><persName><forename type="first">Tomas</forename><surname>Hanke</surname></persName><email>tomas.hanke@molecular-medicine.oxford.ac.uk</email><affiliation>Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, OX3 9DS, Oxford, UK</affiliation></author><author xml:id="author-0008"><persName><forename type="first">Leroy</forename><surname>Sharer</surname></persName><email>sharer@umdnj.edu</email><affiliation>Department of Pathology, New Jersey Medical School, 07103, Newark, NJ, USA</affiliation></author><author xml:id="author-0009"><persName><forename type="first">Mary</forename><surname>Potash</surname></persName><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</affiliation></author><author xml:id="author-0010" corresp="yes"><persName><forename type="first">David</forename><surname>Volsky</surname></persName><email>djv4@columbia.edu</email><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</affiliation></author><idno type="istex">4965E275D19BC1D93D36047D12435B604366B022</idno><idno type="ark">ark:/67375/VQC-VNSFD39P-R</idno><idno type="DOI">10.1007/s11481-011-9316-1</idno><idno type="article-id">9316</idno><idno type="article-id">s11481-011-9316-1</idno></analytic><monogr><title level="j">Journal of Neuroimmune Pharmacology</title><title level="j" type="abbrev">J Neuroimmune Pharmacol</title><idno type="pISSN">1557-1890</idno><idno type="eISSN">1557-1904</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">19</idno><idno type="volume-issue-count">4</idno><editor xml:id="book-author-0000"><persName><forename type="first">Howard</forename><surname>Fox</surname></persName></editor><editor xml:id="book-author-0001"><persName><forename type="first">Howard E.</forename><surname>Gendelman</surname></persName></editor><imprint><publisher>Springer US; http://www.springer-ny.com</publisher><pubPlace>Boston</pubPlace><date type="published" when="2012-06-01"></date><biblScope unit="volume">7</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="380">380</biblScope><biblScope unit="page" to="387">387</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2011-07-04</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Infection by some viruses induces immunity to reinfection, providing a means to identify protective epitopes. To investigate resistance to reinfection in an animal model of HIV disease and its control, we employed infection of mice with chimeric HIV, EcoHIV. When immunocompetent mice were infected by intraperitoneal (IP) injection of EcoHIV, they resisted subsequent secondary infection by IP injection, consistent with a systemic antiviral immune response. To investigate the potential role of these responses in restricting neurotropic HIV infection, we established a protocol for efficient EcoHIV expression in the brain following intracranial (IC) inoculation of virus. When mice were inoculated by IP injection and secondarily by IC injection, they also controlled EcoHIV replication in the brain. To investigate their role in EcoHIV antiviral responses, CD8+ T lymphocytes were isolated from spleens of EcoHIV infected and uninfected mice and adoptively transferred to isogenic recipients. Recipients of EcoHIV primed CD8+ cells resisted subsequent EcoHIV infection compared to recipients of cells from uninfected donors. CD8+ spleen cells from EcoHIV-infected mice also mounted modest but significant interferon-γ responses to two HIV Gag peptide pools. These findings suggest EcoHIV-infected mice may serve as a useful system to investigate the induction of anti-HIV protective immunity for eventual translation to human beings.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Mouse model</term></item><item><term>Chimeric HIV</term></item><item><term>Superinfection</term></item><item><term>Adaptive immune responses</term></item><item><term>HIV neuropathogenesis</term></item><item><term>Brain</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Virology</term></item><item><term>Pharmacology/Toxicology</term></item><item><term>Cell Biology</term></item><item><term>Immunology</term></item><item><term>Neurosciences</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2011-07-04">Created</change><change 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TocLevels="0"><IssueIDStart>2</IssueIDStart><IssueIDEnd>2</IssueIDEnd><IssueTitle Language="En">Special Issue on Animal Models of NeuroAIDS</IssueTitle><IssueArticleCount>19</IssueArticleCount><IssueHistory><OnlineDate><Year>2012</Year><Month>5</Month><Day>16</Day></OnlineDate><PrintDate><Year>2012</Year><Month>5</Month><Day>15</Day></PrintDate><CoverDate><Year>2012</Year><Month>6</Month></CoverDate><PricelistYear>2012</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Springer Science+Business Media, LLC</CopyrightHolderName><CopyrightYear>2012</CopyrightYear></IssueCopyright></IssueInfo><IssueHeader><EditorGroup><Editor><EditorName DisplayOrder="Western"><GivenName>Howard</GivenName><FamilyName>Fox</FamilyName></EditorName></Editor><Editor><EditorName DisplayOrder="Western"><GivenName>Howard E.</GivenName><FamilyName>Gendelman</FamilyName></EditorName></Editor></EditorGroup></IssueHeader><Article ID="s11481-011-9316-1" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>9316</ArticleID><ArticleDOI>10.1007/s11481-011-9316-1</ArticleDOI><ArticleSequenceNumber>9</ArticleSequenceNumber><ArticleTitle Language="En">Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: A Model of Protective Immunity to HIV</ArticleTitle><ArticleCategory>ORIGINAL ARTICLE</ArticleCategory><ArticleFirstPage>380</ArticleFirstPage><ArticleLastPage>387</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2011</Year><Month>9</Month><Day>12</Day></RegistrationDate><Received><Year>2011</Year><Month>7</Month><Day>4</Day></Received><Accepted><Year>2011</Year><Month>9</Month><Day>11</Day></Accepted><OnlineDate><Year>2011</Year><Month>10</Month><Day>11</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer Science+Business Media, LLC</CopyrightHolderName><CopyrightYear>2011</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Jennifer</GivenName><GivenName>L.</GivenName><FamilyName>Kelschenbach</FamilyName></AuthorName><Contact><Email>jlk2152@columbia.edu</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Manisha</GivenName><FamilyName>Saini</FamilyName></AuthorName><Contact><Email>manisaini@gmail.com</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Eran</GivenName><FamilyName>Hadas</FamilyName></AuthorName><Contact><Email>eran.hadas@gmail.com</Email></Contact></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Chao-jiang</GivenName><FamilyName>Gu</FamilyName></AuthorName><Contact><Email>cg2447@columbia.edu</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Wei</GivenName><FamilyName>Chao</FamilyName></AuthorName><Contact><Email>wei616@verizon.net</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Galina</GivenName><FamilyName>Bentsman</FamilyName></AuthorName><Contact><Email>galina_bentsman@yahoo.com</Email></Contact></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Jessie</GivenName><GivenName>P.</GivenName><FamilyName>Hong</FamilyName></AuthorName><Contact><Email>jessie.hong@gmail.com</Email></Contact></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Tomas</GivenName><FamilyName>Hanke</FamilyName></AuthorName><Contact><Email>tomas.hanke@molecular-medicine.oxford.ac.uk</Email></Contact></Author><Author AffiliationIDS="Aff4"><AuthorName DisplayOrder="Western"><GivenName>Leroy</GivenName><GivenName>R.</GivenName><FamilyName>Sharer</FamilyName></AuthorName><Contact><Email>sharer@umdnj.edu</Email></Contact></Author><Author AffiliationIDS="Aff1 Aff2"><AuthorName DisplayOrder="Western"><GivenName>Mary</GivenName><GivenName>Jane</GivenName><FamilyName>Potash</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1 Aff2" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>David</GivenName><GivenName>J.</GivenName><FamilyName>Volsky</FamilyName></AuthorName><Contact><Phone>+1-212-5824451</Phone><Fax>+1-212-5825027</Fax><Email>djv4@columbia.edu</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Molecular Virology Division</OrgDivision><OrgName>St. Luke’s-Roosevelt Hospital Center</OrgName><OrgAddress><Street>432 West 58th Street, Antenucci Research Building, Room 709</Street><City>New York</City><State>NY</State><Postcode>10019</Postcode><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Pathology & Cell Biology</OrgDivision><OrgName>Columbia University</OrgName><OrgAddress><City>New York</City><State>NY</State><Postcode>10032</Postcode><Country Code="US">USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Weatherall Institute of Molecular Medicine</OrgDivision><OrgName>University of Oxford</OrgName><OrgAddress><Street>The John Radcliffe</Street><City>Oxford</City><Postcode>OX3 9DS</Postcode><Country Code="GB">UK</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgDivision>Department of Pathology</OrgDivision><OrgName>New Jersey Medical School</OrgName><OrgAddress><City>Newark</City><State>NJ</State><Postcode>07103</Postcode><Country Code="US">USA</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En" OutputMedium="All"><Heading>Abstract</Heading><Para>Infection by some viruses induces immunity to reinfection, providing a means to identify protective epitopes. To investigate resistance to reinfection in an animal model of HIV disease and its control, we employed infection of mice with chimeric HIV, EcoHIV. When immunocompetent mice were infected by intraperitoneal (IP) injection of EcoHIV, they resisted subsequent secondary infection by IP injection, consistent with a systemic antiviral immune response. To investigate the potential role of these responses in restricting neurotropic HIV infection, we established a protocol for efficient EcoHIV expression in the brain following intracranial (IC) inoculation of virus. When mice were inoculated by IP injection and secondarily by IC injection, they also controlled EcoHIV replication in the brain. To investigate their role in EcoHIV antiviral responses, CD8+ T lymphocytes were isolated from spleens of EcoHIV infected and uninfected mice and adoptively transferred to isogenic recipients. Recipients of EcoHIV primed CD8+ cells resisted subsequent EcoHIV infection compared to recipients of cells from uninfected donors. CD8+ spleen cells from EcoHIV-infected mice also mounted modest but significant interferon-γ responses to two HIV Gag peptide pools. These findings suggest EcoHIV-infected mice may serve as a useful system to investigate the induction of anti-HIV protective immunity for eventual translation to human beings.</Para></Abstract><KeywordGroup Language="En" OutputMedium="All"><Heading>Keywords</Heading><Keyword>Mouse model</Keyword><Keyword>Chimeric HIV</Keyword><Keyword>Superinfection</Keyword><Keyword>Adaptive immune responses</Keyword><Keyword>HIV neuropathogenesis</Keyword><Keyword>Brain</Keyword></KeywordGroup><ArticleNote Type="Misc"><SimplePara>Jennifer L. Kelschenbach and Manisha Saini contributed equally to this work.</SimplePara></ArticleNote><ArticleNote Type="Misc"><SimplePara>Work in this manuscript was supported by grants AI 079792, DA 017618, MH 083627, and NS 061646-01A1 from the National Institutes of Health, Public Health Service.</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: A Model of Protective Immunity to HIV</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: A Model of Protective Immunity to HIV</title></titleInfo><name type="personal"><namePart type="given">Jennifer</namePart><namePart type="given">L.</namePart><namePart type="family">Kelschenbach</namePart><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</affiliation><affiliation>E-mail: jlk2152@columbia.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Manisha</namePart><namePart type="family">Saini</namePart><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>E-mail: manisaini@gmail.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Eran</namePart><namePart type="family">Hadas</namePart><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>E-mail: eran.hadas@gmail.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Chao-jiang</namePart><namePart type="family">Gu</namePart><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</affiliation><affiliation>E-mail: cg2447@columbia.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wei</namePart><namePart type="family">Chao</namePart><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>E-mail: wei616@verizon.net</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Galina</namePart><namePart type="family">Bentsman</namePart><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>E-mail: galina_bentsman@yahoo.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jessie</namePart><namePart type="given">P.</namePart><namePart type="family">Hong</namePart><affiliation>Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, OX3 9DS, Oxford, UK</affiliation><affiliation>E-mail: jessie.hong@gmail.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tomas</namePart><namePart type="family">Hanke</namePart><affiliation>Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, OX3 9DS, Oxford, UK</affiliation><affiliation>E-mail: tomas.hanke@molecular-medicine.oxford.ac.uk</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Leroy</namePart><namePart type="given">R.</namePart><namePart type="family">Sharer</namePart><affiliation>Department of Pathology, New Jersey Medical School, 07103, Newark, NJ, USA</affiliation><affiliation>E-mail: sharer@umdnj.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mary</namePart><namePart type="given">Jane</namePart><namePart type="family">Potash</namePart><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">David</namePart><namePart type="given">J.</namePart><namePart type="family">Volsky</namePart><affiliation>Molecular Virology Division, St. Luke’s-Roosevelt Hospital Center, 432 West 58th Street, Antenucci Research Building, Room 709, 10019, New York, NY, USA</affiliation><affiliation>Department of Pathology & Cell Biology, Columbia University, 10032, New York, NY, USA</affiliation><affiliation>E-mail: djv4@columbia.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Springer US; http://www.springer-ny.com</publisher><place><placeTerm type="text">Boston</placeTerm></place><dateCreated encoding="w3cdtf">2011-07-04</dateCreated><dateIssued encoding="w3cdtf">2012-06-01</dateIssued><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Abstract: Infection by some viruses induces immunity to reinfection, providing a means to identify protective epitopes. To investigate resistance to reinfection in an animal model of HIV disease and its control, we employed infection of mice with chimeric HIV, EcoHIV. When immunocompetent mice were infected by intraperitoneal (IP) injection of EcoHIV, they resisted subsequent secondary infection by IP injection, consistent with a systemic antiviral immune response. To investigate the potential role of these responses in restricting neurotropic HIV infection, we established a protocol for efficient EcoHIV expression in the brain following intracranial (IC) inoculation of virus. When mice were inoculated by IP injection and secondarily by IC injection, they also controlled EcoHIV replication in the brain. To investigate their role in EcoHIV antiviral responses, CD8+ T lymphocytes were isolated from spleens of EcoHIV infected and uninfected mice and adoptively transferred to isogenic recipients. Recipients of EcoHIV primed CD8+ cells resisted subsequent EcoHIV infection compared to recipients of cells from uninfected donors. CD8+ spleen cells from EcoHIV-infected mice also mounted modest but significant interferon-γ responses to two HIV Gag peptide pools. These findings suggest EcoHIV-infected mice may serve as a useful system to investigate the induction of anti-HIV protective immunity for eventual translation to human beings.</abstract><note>ORIGINAL ARTICLE</note><subject lang="en"><genre>Keywords</genre><topic>Mouse model</topic><topic>Chimeric HIV</topic><topic>Superinfection</topic><topic>Adaptive immune responses</topic><topic>HIV neuropathogenesis</topic><topic>Brain</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Neuroimmune Pharmacology</title></titleInfo><titleInfo type="abbreviated"><title>J Neuroimmune Pharmacol</title></titleInfo><name type="personal"><namePart type="given">Howard</namePart><namePart type="family">Fox</namePart><role><roleTerm type="text">editor</roleTerm></role></name><name type="personal"><namePart type="given">Howard E.</namePart><namePart type="family">Gendelman</namePart><role><roleTerm type="text">editor</roleTerm></role></name><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2012-05-16</dateIssued><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Virology</topic><topic>Pharmacology/Toxicology</topic><topic>Cell Biology</topic><topic>Immunology</topic><topic>Neurosciences</topic></subject><identifier type="ISSN">1557-1890</identifier><identifier type="eISSN">1557-1904</identifier><identifier type="JournalID">11481</identifier><identifier type="IssueArticleCount">19</identifier><identifier type="VolumeIssueCount">4</identifier><part><date>2012</date><detail type="issue"><title>Special Issue on Animal Models of NeuroAIDS</title></detail><detail type="volume"><number>7</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="pages"><start>380</start><end>387</end></extent></part><recordInfo><recordOrigin>Springer Science+Business Media, LLC, 2012</recordOrigin></recordInfo></relatedItem><identifier type="istex">4965E275D19BC1D93D36047D12435B604366B022</identifier><identifier type="ark">ark:/67375/VQC-VNSFD39P-R</identifier><identifier type="DOI">10.1007/s11481-011-9316-1</identifier><identifier type="ArticleID">9316</identifier><identifier type="ArticleID">s11481-011-9316-1</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer Science+Business Media, LLC, 2011</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Springer Science+Business Media, LLC, 2011</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-VNSFD39P-R/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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