Cloning of the Rat Insulin-like Growth Factor Binding Protein-5 Gene and DNA Sequence Analysis of Its Promoter Region
Identifieur interne : 001086 ( Istex/Corpus ); précédent : 001085; suivant : 001087Cloning of the Rat Insulin-like Growth Factor Binding Protein-5 Gene and DNA Sequence Analysis of Its Promoter Region
Auteurs : X. Zhu ; N. Ling ; S. ShimasakiSource :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 1993.
Abstract
Abstract: To understand the regulation of the insulin-like growth factor binding protein-5 (IGFBP-5) gene expression, we have cloned the IGFBP-5 gene from rat genomic libraries and determined its genomic organization as well as the DNA sequence at the 5′ flanking region of the gene. The rat IGFBP-5 gene spans at least 17 kilobases (kb) of the genome and contains 4 exons interrupted by 3 introns of approximately 10, 0.6 and 0.7 kb, respectively. Southern blot analysis of the rat chromosome DNA revealed a single copy gene for IGFBP-5 in the haploid genome. Primer extension experiments demonstrated a single transcriptional start site located at 772 nucleotides 5 of the ATG translational start codon. In addition to a TATA box and a CAAT box, multiple putative cis-regulatory elements, including an AP- 1, an AP-2 and a binding site for progesterone receptor are present in the promoter region. This finding suggests that the IGFBP-5 gene is one of those that are expressed in a tissue-specific manner.
Url:
DOI: 10.1006/bbrc.1993.1154
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The rat IGFBP-5 gene spans at least 17 kilobases (kb) of the genome and contains 4 exons interrupted by 3 introns of approximately 10, 0.6 and 0.7 kb, respectively. Southern blot analysis of the rat chromosome DNA revealed a single copy gene for IGFBP-5 in the haploid genome. Primer extension experiments demonstrated a single transcriptional start site located at 772 nucleotides 5 of the ATG translational start codon. In addition to a TATA box and a CAAT box, multiple putative cis-regulatory elements, including an AP- 1, an AP-2 and a binding site for progesterone receptor are present in the promoter region. This finding suggests that the IGFBP-5 gene is one of those that are expressed in a tissue-specific manner.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>X. Zhu</name><affiliations><json:string>Whittier Inst Diabet & Endocrinol, Dept Molec Endocrinol, La Jolla, CA 92037, USA</json:string></affiliations></json:item><json:item><name>N. 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The rat IGFBP-5 gene spans at least 17 kilobases (kb) of the genome and contains 4 exons interrupted by 3 introns of approximately 10, 0.6 and 0.7 kb, respectively. Southern blot analysis of the rat chromosome DNA revealed a single copy gene for IGFBP-5 in the haploid genome. Primer extension experiments demonstrated a single transcriptional start site located at 772 nucleotides 5 of the ATG translational start codon. In addition to a TATA box and a CAAT box, multiple putative cis-regulatory elements, including an AP- 1, an AP-2 and a binding site for progesterone receptor are present in the promoter region. 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The rat IGFBP-5 gene spans at least 17 kilobases (kb) of the genome and contains 4 exons interrupted by 3 introns of approximately 10, 0.6 and 0.7 kb, respectively. Southern blot analysis of the rat chromosome DNA revealed a single copy gene for IGFBP-5 in the haploid genome. Primer extension experiments demonstrated a single transcriptional start site located at 772 nucleotides 5 of the ATG translational start codon. In addition to a TATA box and a CAAT box, multiple putative cis-regulatory elements, including an AP- 1, an AP-2 and a binding site for progesterone receptor are present in the promoter region. 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