Antisense effect of oligodeoxynucleotides complementary to the mini-exon sequence of the protozoan parasite Leishmania amazonensis
Identifieur interne : 001055 ( Istex/Corpus ); précédent : 001054; suivant : 001056Antisense effect of oligodeoxynucleotides complementary to the mini-exon sequence of the protozoan parasite Leishmania amazonensis
Auteurs : E. Pascolo ; C. Blonski ; D. Shire ; J. J. ToulméSource :
- Biochimie [ 0300-9084 ] ; 1993.
English descriptors
- Teeft :
- Acridine, Acridine residue, Amazonensis, Amazonensis mrna, Antisense, Antisense oligodeoxynucleotides, Antisense oligomers, Antisense oligonucleotides, Base pairs, Duplex, Gene expression, Hybrid, Hybrid stability, Intercalating, Intercalating agent, Intercalating agents, Leishmania, Leishmania amazonensis, Mrna, Nucleic, Nucleic acids, Oligodeoxynucleotides, Oligodeoxynucleotides covalently, Oligomer, Oligomers, Oligonucleotide, Oligonucleotides, Parasite, Protein synthesis, Target sequences, Thuong, Toulm6, Unmodified, Wheat germ.
Abstract
Abstract: We have targeted the mini-exon of Leishmania amazonensis, the sequence present at the 5′ end of every mRNA of this protozoan parasite, with a complementary 12-mer, either unmodified (12 Le II) or linked to an acridine derivative (12 Le II Acr). Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited in vitro translation of L amazonensis mRNAs, in a wheat germ extract, to the same extent. Those results indicated that the intercalating agent did not stabilize the duplex formed by the antisense oligomer and its target sequence.
Url:
DOI: 10.1016/0300-9084(93)90023-L
Links to Exploration step
ISTEX:E0FF439C419FF267B3E862AE84E501EEBEC289C2Le document en format XML
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Blonski</name><affiliation><mods:affiliation>Sanofi Elf BioRecherche, 31676 Labège France</mods:affiliation></affiliation><affiliation><mods:affiliation>∗∗Present address: Université Paul Sabatier, 31062 Toulouse, France.</mods:affiliation></affiliation></author><author><name sortKey="Shire, D" sort="Shire, D" uniqKey="Shire D" first="D." last="Shire">D. Shire</name><affiliation><mods:affiliation>Sanofi Elf BioRecherche, 31676 Labège France</mods:affiliation></affiliation></author><author><name sortKey="Toulme, J J" sort="Toulme, J J" uniqKey="Toulme J" first="J. J." last="Toulmé">J. J. 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Pascolo</name><affiliation><mods:affiliation>Laboratoire de Biophysique Moléculaire, INSERM CJF 90-13, Université de Bordeaux II, 146, rue Léo-Saignat, 33706 Bordeaux, France</mods:affiliation></affiliation></author><author><name sortKey="Blonski, C" sort="Blonski, C" uniqKey="Blonski C" first="C." last="Blonski">C. Blonski</name><affiliation><mods:affiliation>Sanofi Elf BioRecherche, 31676 Labège France</mods:affiliation></affiliation><affiliation><mods:affiliation>∗∗Present address: Université Paul Sabatier, 31062 Toulouse, France.</mods:affiliation></affiliation></author><author><name sortKey="Shire, D" sort="Shire, D" uniqKey="Shire D" first="D." last="Shire">D. Shire</name><affiliation><mods:affiliation>Sanofi Elf BioRecherche, 31676 Labège France</mods:affiliation></affiliation></author><author><name sortKey="Toulme, J J" sort="Toulme, J J" uniqKey="Toulme J" first="J. J." last="Toulmé">J. J. 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Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited in vitro translation of L amazonensis mRNAs, in a wheat germ extract, to the same extent. 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Pascolo</name><affiliations><json:string>Laboratoire de Biophysique Moléculaire, INSERM CJF 90-13, Université de Bordeaux II, 146, rue Léo-Saignat, 33706 Bordeaux, France</json:string></affiliations></json:item><json:item><name>C. Blonski</name><affiliations><json:string>Sanofi Elf BioRecherche, 31676 Labège France</json:string><json:string>∗∗Present address: Université Paul Sabatier, 31062 Toulouse, France.</json:string></affiliations></json:item><json:item><name>D. Shire</name><affiliations><json:string>Sanofi Elf BioRecherche, 31676 Labège France</json:string></affiliations></json:item><json:item><name>J.J. 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Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited in vitro translation of L amazonensis mRNAs, in a wheat germ extract, to the same extent. 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Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited in vitro translation of L amazonensis mRNAs, in a wheat germ extract, to the same extent. Those results indicated that the intercalating agent did not stabilize the duplex formed by the antisense oligomer and its target sequence.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>in vitro translation</term></item><item><term>intercalating agent</term></item><item><term>acridine derivative</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-SP49TT70-9/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>BIOCHI</jid><aid>9390023L</aid><ce:pii>0300-9084(93)90023-L</ce:pii><ce:doi>10.1016/0300-9084(93)90023-L</ce:doi><ce:copyright type="unknown" year="1993"></ce:copyright></item-info><head><ce:title>Antisense effect of oligodeoxynucleotides complementary to the mini-exon sequence of the protozoan parasite <ce:italic>Leishmania amazonensis</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Pascolo</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>C.</ce:given-name><ce:surname>Blonski</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>∗∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>D.</ce:given-name><ce:surname>Shire</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.J.</ce:given-name><ce:surname>Toulmé</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Laboratoire de Biophysique Moléculaire, INSERM CJF 90-13, Université de Bordeaux II, 146, rue Léo-Saignat, 33706 Bordeaux, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Sanofi Elf BioRecherche, 31676 Labège France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence and reprints</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>∗∗</ce:label><ce:note-para>Present address: Université Paul Sabatier, 31062 Toulouse, France.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="30" month="9" year="1992"></ce:date-received><ce:date-accepted day="17" month="11" year="1992"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>We have targeted the mini-exon of <ce:italic>Leishmania amazonensis</ce:italic>, the sequence present at the 5′ end of every mRNA of this protozoan parasite, with a complementary 12-mer, either unmodified (12 Le II) or linked to an acridine derivative (12 Le II Acr). Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited <ce:italic>in vitro</ce:italic> translation of <ce:italic>L amazonensis</ce:italic> mRNAs, in a wheat germ extract, to the same extent. Those results indicated that the intercalating agent did not stabilize the duplex formed by the antisense oligomer and its target sequence.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>in vitro</ce:italic> translation</ce:text></ce:keyword><ce:keyword><ce:text>intercalating agent</ce:text></ce:keyword><ce:keyword><ce:text>acridine derivative</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Antisense effect of oligodeoxynucleotides complementary to the mini-exon sequence of the protozoan parasite Leishmania amazonensis</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Antisense effect of oligodeoxynucleotides complementary to the mini-exon sequence of the protozoan parasite</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Pascolo</namePart><affiliation>Laboratoire de Biophysique Moléculaire, INSERM CJF 90-13, Université de Bordeaux II, 146, rue Léo-Saignat, 33706 Bordeaux, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Blonski</namePart><affiliation>Sanofi Elf BioRecherche, 31676 Labège France</affiliation><affiliation>∗∗Present address: Université Paul Sabatier, 31062 Toulouse, France.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Shire</namePart><affiliation>Sanofi Elf BioRecherche, 31676 Labège France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.J.</namePart><namePart type="family">Toulmé</namePart><affiliation>Correspondence and reprints</affiliation><affiliation>Laboratoire de Biophysique Moléculaire, INSERM CJF 90-13, Université de Bordeaux II, 146, rue Léo-Saignat, 33706 Bordeaux, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: We have targeted the mini-exon of Leishmania amazonensis, the sequence present at the 5′ end of every mRNA of this protozoan parasite, with a complementary 12-mer, either unmodified (12 Le II) or linked to an acridine derivative (12 Le II Acr). Physical measurements performed either in solution or on nitrocellulose filters showed that the two oligomers exhibited the same affinity for both DNA and RNA target sequences. Furthermore, the two oligomers 12 Le II and 12 Le II Acr inhibited in vitro translation of L amazonensis mRNAs, in a wheat germ extract, to the same extent. Those results indicated that the intercalating agent did not stabilize the duplex formed by the antisense oligomer and its target sequence.</abstract><subject><genre>Keywords</genre><topic>in vitro translation</topic><topic>intercalating agent</topic><topic>acridine derivative</topic></subject><relatedItem type="host"><titleInfo><title>Biochimie</title></titleInfo><titleInfo type="abbreviated"><title>BIOCHI</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued></originInfo><identifier type="ISSN">0300-9084</identifier><identifier type="PII">S0300-9084(00)X0088-0</identifier><part><date>1993</date><detail type="volume"><number>75</number><caption>vol.</caption></detail><detail type="issue"><number>1–2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>3</start><end>139</end></extent><extent unit="pages"><start>43</start><end>47</end></extent></part></relatedItem><identifier type="istex">E0FF439C419FF267B3E862AE84E501EEBEC289C2</identifier><identifier type="ark">ark:/67375/6H6-SP49TT70-9</identifier><identifier type="DOI">10.1016/0300-9084(93)90023-L</identifier><identifier type="PII">0300-9084(93)90023-L</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-SP49TT70-9/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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