A polymerase chain reaction assay adapted to plum pox potyvirus detection
Identifieur interne : 000F88 ( Istex/Corpus ); précédent : 000F87; suivant : 000F89A polymerase chain reaction assay adapted to plum pox potyvirus detection
Auteurs : T. Wetzel ; T. Candresse ; M. Ravelonandro ; J. DunezSource :
- Journal of Virological Methods [ 0166-0934 ] ; 1991.
English descriptors
- Teeft :
- Alui restriction site, Amar, Amar strain, Amplification, Annealing temperatures, Assay, Chemical denaturation, Clone, Clone pbppv1, Complete nucleotide sequence, Crude plant extracts, Detection limit, Dunez, Elisa test, Enzymatic amplification, Ethidium bromide staining, Field indexing trial, Fragment, Healthy peach extracts, Healthy sample, Hybridization, Hybridization detection, Lower amounts, Lower detection level, Methyl mercury hydroxide, Molecular hybridization, Molecular hybridization assay, Molecular weight standards, Nucleic acids, Nucleotide sequence, Pbppv1, Peach, Peach seedlings, Plant extracts, Plum, Polymerase, Polymerase chain reaction, Potyvirus, Primer, Probe pbppv1, Protein genes, Restriction analysis, Restriction endonucleases, Restriction profile, Restriction sites, Room temperature, Rsai, Silver staining, Small strips, Southern france, Sterile water, Target copies, Target sequence, Teycheney, Transcription mixture, Varveri, Viral, Viral particles, Virol, Wetzel.
Abstract
Abstract: A sensitive, polyvalent assay based on the polymerase chain reaction (PCR) was developed for plum pox potyvirus (PPV) detection. This technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral RNA followed by the PCR reaction yielding a 243-base-pair product. As few as 10 fg of purified viral RNA, corresponding to approximately 2000 viral particles, were detected in plant extracts. All PPV isolates tested were amplified, and the amplified fragments were analysed by restriction endonuclease digestion. An Rsal restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. In a field indexing trial, the PCR assay proved to be more sensitive than molecular hybridization using 32P-labelled RNA probes for PPV detection.
Url:
DOI: 10.1016/0166-0934(91)90035-X
Links to Exploration step
ISTEX:BB7D398E9B016DABC9E669D31290DFD69A67F639Le document en format XML
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Wetzel</name><affiliation><mods:affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Candresse, T" sort="Candresse, T" uniqKey="Candresse T" first="T." last="Candresse">T. Candresse</name><affiliation><mods:affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Ravelonandro, M" sort="Ravelonandro, M" uniqKey="Ravelonandro M" first="M." last="Ravelonandro">M. Ravelonandro</name><affiliation><mods:affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Dunez, J" sort="Dunez, J" uniqKey="Dunez J" first="J." last="Dunez">J. 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This technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral RNA followed by the PCR reaction yielding a 243-base-pair product. As few as 10 fg of purified viral RNA, corresponding to approximately 2000 viral particles, were detected in plant extracts. All PPV isolates tested were amplified, and the amplified fragments were analysed by restriction endonuclease digestion. An Rsal restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. 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Wetzel</name><affiliations><json:string>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</json:string></affiliations></json:item><json:item><name>T. Candresse</name><affiliations><json:string>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</json:string></affiliations></json:item><json:item><name>M. Ravelonandro</name><affiliations><json:string>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</json:string></affiliations></json:item><json:item><name>J. Dunez</name><affiliations><json:string>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Polymerase chain reaction, PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Plum pox potyvirus, PPV</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Polymorphism</value></json:item></subject><arkIstex>ark:/67375/6H6-00P36L1Z-W</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A sensitive, polyvalent assay based on the polymerase chain reaction (PCR) was developed for plum pox potyvirus (PPV) detection. This technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral RNA followed by the PCR reaction yielding a 243-base-pair product. As few as 10 fg of purified viral RNA, corresponding to approximately 2000 viral particles, were detected in plant extracts. All PPV isolates tested were amplified, and the amplified fragments were analysed by restriction endonuclease digestion. An Rsal restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. In a field indexing trial, the PCR assay proved to be more sensitive than molecular hybridization using 32P-labelled RNA probes for PPV detection.</abstract><qualityIndicators><score>7.223</score><pdfWordCount>3747</pdfWordCount><pdfCharCount>22032</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>11</pdfPageCount><pdfPageSize>432 x 648 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>123</abstractWordCount><abstractCharCount>828</abstractCharCount><keywordCount>3</keywordCount></qualityIndicators><title>A polymerase chain reaction assay adapted to plum pox potyvirus detection</title><pmid><json:string>1783677</json:string></pmid><pii><json:string>0166-0934(91)90035-X</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>European Handbook of Plant diseases</title><language><json:string>unknown</json:string></language><pages><first>35</first></pages></serie><host><title>Journal of Virological Methods</title><language><json:string>unknown</json:string></language><publicationDate>1991</publicationDate><issn><json:string>0166-0934</json:string></issn><pii><json:string>S0166-0934(00)X0112-7</json:string></pii><volume>33</volume><issue>3</issue><pages><first>355</first><last>365</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>2000</json:string><json:string>1991</json:string></date><geogName></geogName><orgName><json:string>Cyprus and Turkey</json:string><json:string>Italy and Germany</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>T. Candresse</json:string><json:string>M. Ravelonandro</json:string><json:string>T. Wetzel</json:string><json:string>Europe</json:string><json:string>J. Dunez</json:string><json:string>El Amar</json:string><json:string>D. 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(1988)</json:string><json:string>Maiss et al., 1989</json:string><json:string>Varveri et al.</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-00P36L1Z-W</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string><json:string>2 - biotechnology & applied microbiology</json:string><json:string>2 - biochemical research methods</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1991</publicationDate><copyrightDate>1991</copyrightDate><doi><json:string>10.1016/0166-0934(91)90035-X</json:string></doi><id>BB7D398E9B016DABC9E669D31290DFD69A67F639</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-00P36L1Z-W/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-00P36L1Z-W/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-00P36L1Z-W/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">A polymerase chain reaction assay adapted to plum pox potyvirus detection</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1991</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">A polymerase chain reaction assay adapted to plum pox potyvirus detection</title><author xml:id="author-0000"><persName><forename type="first">T.</forename><surname>Wetzel</surname></persName><note type="correspondence"><p>Correspondence to: T. Wetzel, Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France.</p></note><affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</affiliation></author><author xml:id="author-0001"><persName><forename type="first">T.</forename><surname>Candresse</surname></persName><affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</affiliation></author><author xml:id="author-0002"><persName><forename type="first">M.</forename><surname>Ravelonandro</surname></persName><affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</affiliation></author><author xml:id="author-0003"><persName><forename type="first">J.</forename><surname>Dunez</surname></persName><affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</affiliation></author><idno type="istex">BB7D398E9B016DABC9E669D31290DFD69A67F639</idno><idno type="ark">ark:/67375/6H6-00P36L1Z-W</idno><idno type="DOI">10.1016/0166-0934(91)90035-X</idno><idno type="PII">0166-0934(91)90035-X</idno></analytic><monogr><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="pISSN">0166-0934</idno><idno type="PII">S0166-0934(00)X0112-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991"></date><biblScope unit="volume">33</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="355">355</biblScope><biblScope unit="page" to="365">365</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1991</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A sensitive, polyvalent assay based on the polymerase chain reaction (PCR) was developed for plum pox potyvirus (PPV) detection. This technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral RNA followed by the PCR reaction yielding a 243-base-pair product. As few as 10 fg of purified viral RNA, corresponding to approximately 2000 viral particles, were detected in plant extracts. All PPV isolates tested were amplified, and the amplified fragments were analysed by restriction endonuclease digestion. An Rsal restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. In a field indexing trial, the PCR assay proved to be more sensitive than molecular hybridization using 32P-labelled RNA probes for PPV detection.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Polymerase chain reaction, PCR</term></item><item><term>Plum pox potyvirus, PPV</term></item><item><term>Polymorphism</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1991">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-00P36L1Z-W/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VIRMET</jid><aid>9190035X</aid><ce:pii>0166-0934(91)90035-X</ce:pii><ce:doi>10.1016/0166-0934(91)90035-X</ce:doi><ce:copyright type="unknown" year="1991"></ce:copyright></item-info><head><ce:title>A polymerase chain reaction assay adapted to plum pox potyvirus detection</ce:title><ce:author-group><ce:author><ce:given-name>T.</ce:given-name><ce:surname>Wetzel</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>T.</ce:given-name><ce:surname>Candresse</ce:surname></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Ravelonandro</ce:surname></ce:author><ce:author><ce:given-name>J.</ce:given-name><ce:surname>Dunez</ce:surname></ce:author><ce:affiliation><ce:textfn>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: T. Wetzel, Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="22" month="3" year="1991"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A sensitive, polyvalent assay based on the polymerase chain reaction (PCR) was developed for plum pox potyvirus (PPV) detection. This technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral RNA followed by the PCR reaction yielding a 243-base-pair product. As few as 10 fg of purified viral RNA, corresponding to approximately 2000 viral particles, were detected in plant extracts. All PPV isolates tested were amplified, and the amplified fragments were analysed by restriction endonuclease digestion. An <ce:italic>Rsal</ce:italic> restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. In a field indexing trial, the PCR assay proved to be more sensitive than molecular hybridization using <ce:sup loc="pre">32</ce:sup>P-labelled RNA probes for PPV detection.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Polymerase chain reaction, PCR</ce:text></ce:keyword><ce:keyword><ce:text>Plum pox potyvirus, PPV</ce:text></ce:keyword><ce:keyword><ce:text>Polymorphism</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>A polymerase chain reaction assay adapted to plum pox potyvirus detection</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>A polymerase chain reaction assay adapted to plum pox potyvirus detection</title></titleInfo><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Wetzel</namePart><affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</affiliation><description>Correspondence to: T. Wetzel, Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Candresse</namePart><affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Ravelonandro</namePart><affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Dunez</namePart><affiliation>Station de Pathologie Végétale, INRA, BP 81, 33883 Villenave d'Ornon Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1991</dateIssued><copyrightDate encoding="w3cdtf">1991</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A sensitive, polyvalent assay based on the polymerase chain reaction (PCR) was developed for plum pox potyvirus (PPV) detection. This technique was adapted for a single tube, the chemical denaturation and reverse transcription of the viral RNA followed by the PCR reaction yielding a 243-base-pair product. As few as 10 fg of purified viral RNA, corresponding to approximately 2000 viral particles, were detected in plant extracts. All PPV isolates tested were amplified, and the amplified fragments were analysed by restriction endonuclease digestion. An Rsal restriction site polymorphism in the amplified fragments allowed the discrimination of two groups of isolates. In a field indexing trial, the PCR assay proved to be more sensitive than molecular hybridization using 32P-labelled RNA probes for PPV detection.</abstract><subject><genre>Keywords</genre><topic>Polymerase chain reaction, PCR</topic><topic>Plum pox potyvirus, PPV</topic><topic>Polymorphism</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Virological Methods</title></titleInfo><titleInfo type="abbreviated"><title>VIRMET</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1991</dateIssued></originInfo><identifier type="ISSN">0166-0934</identifier><identifier type="PII">S0166-0934(00)X0112-7</identifier><part><date>1991</date><detail type="volume"><number>33</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>233</start><end>405</end></extent><extent unit="pages"><start>355</start><end>365</end></extent></part></relatedItem><identifier type="istex">BB7D398E9B016DABC9E669D31290DFD69A67F639</identifier><identifier type="ark">ark:/67375/6H6-00P36L1Z-W</identifier><identifier type="DOI">10.1016/0166-0934(91)90035-X</identifier><identifier type="PII">0166-0934(91)90035-X</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-00P36L1Z-W/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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